##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_35008-1.341000000357b4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2434739 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66441207866634 33.0 33.0 33.0 33.0 33.0 2 32.71226073924146 33.0 33.0 33.0 33.0 33.0 3 32.74331047393581 33.0 33.0 33.0 33.0 33.0 4 36.66543395411171 37.0 37.0 37.0 37.0 37.0 5 36.674777050024666 37.0 37.0 37.0 37.0 37.0 6 36.55966532757721 37.0 37.0 37.0 37.0 37.0 7 36.50877773757269 37.0 37.0 37.0 37.0 37.0 8 36.45218481323871 37.0 37.0 37.0 37.0 37.0 9 36.46908600880834 37.0 37.0 37.0 37.0 37.0 10-11 36.42423150900363 37.0 37.0 37.0 37.0 37.0 12-13 36.392315767727055 37.0 37.0 37.0 37.0 37.0 14-15 38.25908629220627 40.0 37.0 40.0 37.0 40.0 16-17 38.24422371350687 40.0 37.0 40.0 37.0 40.0 18-19 38.268331225646776 40.0 37.0 40.0 37.0 40.0 20-21 38.231783981773816 40.0 37.0 40.0 37.0 40.0 22-23 38.144632135107706 40.0 37.0 40.0 37.0 40.0 24-25 38.11071350974375 40.0 37.0 40.0 37.0 40.0 26-27 38.00197618718064 40.0 37.0 40.0 37.0 40.0 28-29 37.908929047425616 40.0 37.0 40.0 33.0 40.0 30-31 37.862581985173776 40.0 37.0 40.0 33.0 40.0 32-33 37.75411881930671 40.0 37.0 40.0 33.0 40.0 34-35 37.679095582729815 40.0 37.0 40.0 33.0 40.0 36-37 37.60274099195027 40.0 37.0 40.0 33.0 40.0 38-39 37.497122689536745 40.0 37.0 40.0 33.0 40.0 40-41 37.41298245931083 40.0 37.0 40.0 33.0 40.0 42-43 37.26553564879028 40.0 37.0 40.0 33.0 40.0 44-45 37.19282621258377 40.0 37.0 40.0 33.0 40.0 46-47 37.035327811317764 40.0 37.0 40.0 33.0 40.0 48-49 36.94609278448327 37.0 37.0 40.0 33.0 40.0 50-51 36.779514354516024 37.0 37.0 40.0 33.0 40.0 52-53 36.63357694602995 37.0 37.0 40.0 33.0 40.0 54-55 36.510062474868974 37.0 37.0 40.0 33.0 40.0 56-57 36.31130585249589 37.0 37.0 40.0 33.0 40.0 58-59 36.01393948180893 37.0 37.0 40.0 33.0 40.0 60-61 35.78550144389193 37.0 37.0 40.0 30.0 40.0 62-63 35.53649795727591 37.0 33.0 40.0 27.0 40.0 64-65 35.24483158153708 37.0 33.0 37.0 27.0 40.0 66-67 34.927188704826264 37.0 33.0 37.0 27.0 40.0 68-69 34.6232039655996 37.0 33.0 37.0 27.0 40.0 70-71 34.267833431016626 37.0 33.0 37.0 27.0 40.0 72-73 33.90349150360675 37.0 33.0 37.0 27.0 37.0 74-75 33.47787339833962 37.0 33.0 37.0 27.0 37.0 76-77 30.74656831800041 33.0 30.0 35.0 24.5 37.0 78-79 33.18751024237095 35.0 33.0 37.0 27.0 37.0 80-81 33.580080452155244 37.0 33.0 37.0 27.0 37.0 82-83 33.483707083182225 37.0 33.0 37.0 27.0 37.0 84-85 33.35380630120929 37.0 33.0 37.0 27.0 37.0 86-87 33.26818891059781 37.0 33.0 37.0 27.0 37.0 88-89 33.00168252120659 37.0 33.0 37.0 27.0 37.0 90-91 32.98844742701374 37.0 33.0 37.0 27.0 37.0 92-93 32.82826927239429 35.0 33.0 37.0 27.0 37.0 94-95 32.653950587722136 33.0 33.0 37.0 27.0 37.0 96-97 32.54153627965872 33.0 33.0 37.0 27.0 37.0 98-99 32.42325337541314 33.0 33.0 37.0 24.5 37.0 100-101 30.83289748921753 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.13967654603446533 2306 2 0.1361915566459473 2306 3 0.176370192450932 2306 4 0.21365271899578886 2306 5 0.18067017039916777 2306 6 0.22990427433348515 2306 7 0.254300887595889 2306 8 0.24761663914960508 2306 9 0.20448417737099334 2306 10-11 0.2353254093278423 2306 12-13 0.21766482115756958 2306 14-15 0.4164046611000245 2306 16-17 0.3904741934191307 2306 18-19 0.46244283749672377 2306 20-21 0.4258823476796536 2306 22-23 0.39375967759789177 2306 24-25 0.4060096525314094 2306 26-27 0.3137580802971769 2306 28-29 0.44862437487959994 2306 30-31 0.3174801700979728 2306 32-33 0.4130242063866305 2306 34-35 0.43143127997024067 2306 36-37 0.35745137324745 2306 38-39 0.3945788538111046 2306 40-41 0.5375507157194406 2306 42-43 0.5017305872440474 2306 44-45 0.3105477531996712 2306 46-47 0.4050128290582151 2306 48-49 0.4356866259301384 2306 50-51 0.41674243666850685 2306 52-53 0.6078945083157166 2306 54-55 0.5521458195855615 2306 56-57 0.530760161255948 2306 58-59 0.6125287029982687 2306 60-61 0.5212410683327207 2306 62-63 0.3928618303419782 2306 64-65 0.4851874435791572 2306 66-67 0.3154633844374999 2306 68-69 0.35086460074172265 2306 70-71 0.11722761480390176 2306 72-73 0.20162351517404886 2306 74-75 0.23228518541983334 2306 76-77 -0.1778513655556857 2306 78-79 0.249940458163465 2306 80-81 0.1980885794862317 2306 82-83 0.36204866847526773 2306 84-85 0.4284487310159335 2306 86-87 0.44263012489996356 2306 88-89 0.6841280214936489 2306 90-91 0.44273396426867606 2306 92-93 0.454557163310767 2306 94-95 0.3727654137499812 2306 96-97 0.40781165090013616 2306 98-99 0.3303571362992628 2306 100-101 0.32989542275858597 2307 1 0.16555308984312234 2307 2 0.11403555828947987 2307 3 0.09267376537056293 2307 4 0.16081028124347796 2307 5 0.14433553460393966 2307 6 0.13478965006282806 2307 7 0.21062464366853817 2307 8 0.2371852779910455 2307 9 0.23390655173541575 2307 10-11 0.17249335801788135 2307 12-13 0.26378418009169025 2307 14-15 0.35384212267445747 2307 16-17 0.3709193526453092 2307 18-19 0.36584561678633776 2307 20-21 0.3391488025022156 2307 22-23 0.42904836640668265 2307 24-25 0.33913118768560935 2307 26-27 0.3421054344550214 2307 28-29 0.3996554270077439 2307 30-31 0.34613520435339495 2307 32-33 0.398706265509162 2307 34-35 0.3141209744644371 2307 36-37 0.34812548339333915 2307 38-39 0.3896197340655405 2307 40-41 0.43383965006604797 2307 42-43 0.41884476563346595 2307 44-45 0.3643646260841109 2307 46-47 0.45365425539314685 2307 48-49 0.39613216545057384 2307 50-51 0.3568403045680668 2307 52-53 0.41367451249895026 2307 54-55 0.3297786752454215 2307 56-57 0.26281530171818446 2307 58-59 0.3986366090126765 2307 60-61 0.3975755773631988 2307 62-63 0.32439012547457224 2307 64-65 0.2999051894593663 2307 66-67 0.4151141199899726 2307 68-69 0.2537140243341227 2307 70-71 0.19120876378307372 2307 72-73 0.022864823176846016 2307 74-75 0.11419500111767178 2307 76-77 -0.1922805011661346 2307 78-79 0.1690415362425597 2307 80-81 0.21011304287863197 2307 82-83 0.30683311190498586 2307 84-85 0.3096180752205342 2307 86-87 0.17127405346085567 2307 88-89 0.4084481697312441 2307 90-91 0.3233485748983398 2307 92-93 0.21978222999316444 2307 94-95 0.2247719757548836 2307 96-97 0.18567268311243623 2307 98-99 0.07051998491265721 2307 100-101 0.02483907996024115 2201 1 0.1327613608517595 2201 2 0.10526902714470054 2201 3 0.14951652263702186 2201 4 0.18194563400674468 2201 5 0.17423504632976972 2201 6 0.1736462582376319 2201 7 0.17079491363605825 2201 8 0.2684184832704659 2201 9 0.1647808985616308 2201 10-11 0.2222521202659209 2201 12-13 0.28399701755108 2201 14-15 0.500808035733975 2201 16-17 0.39555999998746927 2201 18-19 0.27180295704292945 2201 20-21 0.3211845302020535 2201 22-23 0.3558942206888034 2201 24-25 0.3602861768460599 2201 26-27 0.332732020028196 2201 28-29 0.3961565900359645 2201 30-31 0.3627805433448188 2201 32-33 0.3771632927694881 2201 34-35 0.411235610169733 2201 36-37 0.3411904087025519 2201 38-39 0.46182131683822547 2201 40-41 0.2700714386255427 2201 42-43 0.31508129855738076 2201 44-45 0.33467544784755887 2201 46-47 0.5126213018286165 2201 48-49 0.36627785697462656 2201 50-51 0.3196824424313931 2201 52-53 0.21610165026343253 2201 54-55 0.3459688188283394 2201 56-57 0.27673961592689267 2201 58-59 0.32603729035612616 2201 60-61 0.19698029141639495 2201 62-63 0.36007802536848743 2201 64-65 0.4523592778495171 2201 66-67 0.26074660307170205 2201 68-69 0.25422127596335287 2201 70-71 0.18904928724462167 2201 72-73 0.27276262165997167 2201 74-75 0.17770912548591156 2201 76-77 -0.14772129874651085 2201 78-79 0.30095331742192855 2201 80-81 0.24401442599265266 2201 82-83 0.41750845900136113 2201 84-85 0.3761459252986157 2201 86-87 0.41625906258717293 2201 88-89 0.6256317305085304 2201 90-91 0.42793677065608193 2201 92-93 0.4423700172084182 2201 94-95 0.4521735796136426 2201 96-97 0.4441298421606632 2201 98-99 0.48389105351485284 2201 100-101 0.4000989327531599 1101 1 0.16030851244882172 1101 2 0.1171199762101196 1101 3 0.12274479308144493 1101 4 0.15111313530393744 1101 5 0.14465437275276116 1101 6 0.07501754614293077 1101 7 0.141983628626825 1101 8 0.17351218311721794 1101 9 0.24031830006517652 1101 10-11 0.16228892445556653 1101 12-13 0.23506108097495115 1101 14-15 0.2882294497388713 1101 16-17 0.3443133636490643 1101 18-19 0.32615647033467354 1101 20-21 0.2956606436796321 1101 22-23 0.28236668994825465 1101 24-25 0.3195512997479142 1101 26-27 0.37672207686352266 1101 28-29 0.32309568815231415 1101 30-31 0.319538395910854 1101 32-33 0.3530862332432392 1101 34-35 0.28202116844705216 1101 36-37 0.30897153841016944 1101 38-39 0.30944521422090787 1101 40-41 0.2948291946560033 1101 42-43 0.25268021735889334 1101 44-45 0.39798046407760523 1101 46-47 0.3748129008198191 1101 48-49 0.41646175725337997 1101 50-51 0.32107026205465417 1101 52-53 0.2326905655655338 1101 54-55 0.2820230476900676 1101 56-57 0.35412000231054463 1101 58-59 0.4164145031326072 1101 60-61 0.45342597205257107 1101 62-63 0.5268192255470794 1101 64-65 0.5127987560747087 1101 66-67 0.4144739624615923 1101 68-69 0.4146770445675685 1101 70-71 0.3384221679869768 1101 72-73 0.30351149641712283 1101 74-75 0.2971995382961765 1101 76-77 -0.03308759093557612 1101 78-79 0.2116223089871383 1101 80-81 0.37149315799781135 1101 82-83 0.4990252742925989 1101 84-85 0.5430191378820268 1101 86-87 0.35692383072078115 1101 88-89 0.5479279499339071 1101 90-91 0.2863064053895883 1101 92-93 0.2620590519732957 1101 94-95 0.5465062170863391 1101 96-97 0.524241702763284 1101 98-99 0.4353267789193964 1101 100-101 0.501818593430599 2304 1 0.0631488848652495 2304 2 0.0740639708182016 2304 3 0.09716334589194275 2304 4 0.13425946055245674 2304 5 0.1010441398242179 2304 6 0.13240260621411437 2304 7 0.13223241472427816 2304 8 0.11546274934281797 2304 9 0.11914418563817009 2304 10-11 0.11862853568330678 2304 12-13 0.08234593425982695 2304 14-15 0.1683342546905564 2304 16-17 0.15238192002183126 2304 18-19 0.2801229124692526 2304 20-21 0.14396434900541522 2304 22-23 0.15935680607815073 2304 24-25 0.19715661718523592 2304 26-27 0.10452117724907595 2304 28-29 0.15689681487766904 2304 30-31 0.2198117507226911 2304 32-33 0.08362554733857763 2304 34-35 0.16075406355599142 2304 36-37 0.24750768274360269 2304 38-39 0.16143280616782363 2304 40-41 0.1863234191942098 2304 42-43 0.14903529712962893 2304 44-45 0.15338942761529495 2304 46-47 0.20170202777595136 2304 48-49 0.07915059564404459 2304 50-51 0.10465763091188052 2304 52-53 0.14568951786569073 2304 54-55 0.1514000749586728 2304 56-57 0.025905078291735606 2304 58-59 0.15667530914903693 2304 60-61 -0.13123960539089552 2304 62-63 -0.07515700211281029 2304 64-65 -0.11271411201345671 2304 66-67 -0.036850829298778365 2304 68-69 0.005454422992649199 2304 70-71 -0.26298223934578147 2304 72-73 -0.19600759755198993 2304 74-75 -0.14725062471577388 2304 76-77 -0.3348573819507088 2304 78-79 -0.1575101675663717 2304 80-81 0.021347782541447202 2304 82-83 0.1427373924252393 2304 84-85 0.21278049659618858 2304 86-87 0.1477782240448704 2304 88-89 0.4250202353750723 2304 90-91 0.2604912254120606 2304 92-93 0.23612563970187495 2304 94-95 0.0804933028853938 2304 96-97 0.057042859647701505 2304 98-99 0.24090601007292634 2304 100-101 0.2964264796338725 2202 1 0.061249622715365604 2202 2 0.10264258427401529 2202 3 0.017084693049106647 2202 4 0.0918681232723415 2202 5 0.030018239325919183 2202 6 0.024542371548285757 2202 7 0.10518625920010294 2202 8 0.12457528915905414 2202 9 0.07644542764209916 2202 10-11 0.09088926965531385 2202 12-13 0.08925492197309381 2202 14-15 0.06948031588787984 2202 16-17 0.09454622649725763 2202 18-19 0.0203915925194309 2202 20-21 0.08832220934107227 2202 22-23 0.1912173679591973 2202 24-25 0.20204606085877685 2202 26-27 0.10320544890234373 2202 28-29 0.29174907316762244 2202 30-31 0.274640800970424 2202 32-33 0.18399315949563544 2202 34-35 0.27780339023999545 2202 36-37 0.31188242900032037 2202 38-39 0.2945776877744848 2202 40-41 0.19734239161723366 2202 42-43 0.24610399414651596 2202 44-45 0.257568996054772 2202 46-47 0.39408508368329365 2202 48-49 0.3504450348032151 2202 50-51 0.25920622540133564 2202 52-53 0.20866117739219447 2202 54-55 0.3283058511151893 2202 56-57 0.4605080874598215 2202 58-59 0.39090373768785724 2202 60-61 0.3432207419823925 2202 62-63 0.32151671381993907 2202 64-65 0.2687809585498968 2202 66-67 0.2921562947016554 2202 68-69 0.277797364738241 2202 70-71 0.22681430146420922 2202 72-73 0.18103340736229967 2202 74-75 0.22000098964695525 2202 76-77 -0.15462309989522893 2202 78-79 0.15637628067403853 2202 80-81 0.27879778627815455 2202 82-83 0.42171522864392585 2202 84-85 0.46116795173033154 2202 86-87 0.3926400362112119 2202 88-89 0.543023131835696 2202 90-91 0.45611108616380847 2202 92-93 0.5069799541960975 2202 94-95 0.5087435902086312 2202 96-97 0.36209671886840766 2202 98-99 0.39783963991980187 2202 100-101 0.3887054124275018 1102 1 0.12215771455051083 1102 2 0.12996790334368313 1102 3 0.07954820855373157 1102 4 0.10171065253950928 1102 5 0.08458724955000463 1102 6 0.07951315586898744 1102 7 0.060896749697747055 1102 8 0.12094415747038312 1102 9 0.12120560819705872 1102 10-11 0.1642861516936165 1102 12-13 0.10392987998901759 1102 14-15 0.23359623501633564 1102 16-17 0.12031256471765772 1102 18-19 0.09390595510089383 1102 20-21 0.15966070120916953 1102 22-23 0.12482588347317858 1102 24-25 0.0321821136248559 1102 26-27 0.10831880124474225 1102 28-29 0.03553233981944004 1102 30-31 0.1472386280952307 1102 32-33 0.21452480809869456 1102 34-35 0.12262554061134523 1102 36-37 0.12858694530812187 1102 38-39 0.278606137724033 1102 40-41 0.2523531474056071 1102 42-43 0.17215425148039287 1102 44-45 0.0602468971689234 1102 46-47 0.1050266878964976 1102 48-49 -0.008754393801375215 1102 50-51 0.07120740114286406 1102 52-53 0.061294052098965324 1102 54-55 0.15651232106357327 1102 56-57 0.2330678153168435 1102 58-59 0.24136111612124012 1102 60-61 0.2718188761479823 1102 62-63 0.2984100227647488 1102 64-65 0.2434983031713429 1102 66-67 0.13780567659485854 1102 68-69 0.17467219507012288 1102 70-71 0.05749872157838354 1102 72-73 -0.028395178508951346 1102 74-75 -0.005854652508247682 1102 76-77 4.672243885117666E-4 1102 78-79 0.15935438904071475 1102 80-81 0.1712868011452926 1102 82-83 0.1477470448088738 1102 84-85 0.12650716626759362 1102 86-87 0.1647985516315913 1102 88-89 0.38294891783201024 1102 90-91 0.14782519749604717 1102 92-93 0.14153617729492396 1102 94-95 0.21476149592839278 1102 96-97 0.05419079316853015 1102 98-99 0.023457887128820687 1102 100-101 0.09075734350808418 2305 1 0.06389585000369635 2305 2 0.057019023271315916 2305 3 0.09231228153677051 2305 4 0.0770910925899102 2305 5 0.08959834418288182 2305 6 0.020347733739754403 2305 7 -0.02980458334012326 2305 8 0.00501517075012714 2305 9 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>>Per sequence quality scores pass #Quality Count 8 6.0 9 33.0 10 37.0 11 22.0 12 33.0 13 72.0 14 184.0 15 402.0 16 933.0 17 1547.0 18 2362.0 19 3046.0 20 4002.0 21 5343.0 22 6925.0 23 8800.0 24 11254.0 25 14488.0 26 18074.0 27 24070.0 28 33556.0 29 45080.0 30 47060.0 31 53474.0 32 70498.0 33 99697.0 34 151869.0 35 265253.0 36 539576.0 37 837695.0 38 189294.0 39 54.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.59871347396587 25.558638303194265 24.734318715514505 15.108329507325365 2 28.73281283948711 23.362997019393045 34.01165381587102 13.892536325248825 3 36.79205334461451 19.503231357827445 26.829079961474246 16.875635336083803 4 27.86748871720842 19.316886845082525 34.74602729889189 18.06959713881716 5 32.20197318891265 18.20700288614098 32.76125284886799 16.82977107607838 6 25.75241124407996 19.402038575798063 38.10806825700825 16.73748192311373 7 31.539602396807215 20.806542302891604 29.659195503090885 17.994659797210296 8 27.53186275818476 28.107530211657185 26.66421328939159 17.696393740766464 9 30.252606131499103 24.575570523164906 26.047761176865365 19.124062168470623 10-11 24.2573509177609 28.747499722761866 29.021595823766905 17.973553535710327 12-13 23.71059074504495 26.88255291429595 29.009084752000113 20.39777158865899 14-15 23.00640848978063 27.403101523407642 29.808246386984393 19.782243599827332 16-17 22.700220806403383 27.62579311228597 30.611649344973863 19.062336736336782 18-19 20.936391950020106 27.94568534861437 31.472921738223274 19.64500096314225 20-21 21.793259975709923 28.087363779033396 31.020388633032127 19.098987612224555 22-23 21.1551114991995 29.493975913511417 30.25241854730265 19.09849403998643 24-25 21.508424516960545 27.39718713176238 31.190345248505075 19.904043102772 26-27 21.37641858121137 27.637870013993286 30.232850420517355 20.752860984277987 28-29 21.39138938506345 28.733942324002697 30.04950427951415 19.825164011419705 30-31 20.165796005239166 28.77622611704992 30.497211405411424 20.560766472299495 32-33 19.998529616521523 29.487205815489876 30.401780231885223 20.11248433610338 34-35 21.044596566613507 28.95189176334712 30.60578567148265 19.397725998556727 36-37 20.139104519787935 29.39371573522333 31.28792023381782 19.179259511170912 38-39 20.796005290136033 29.76808421502267 29.726847440362754 19.70906305447854 40-41 20.684851608981297 28.59343263719315 31.03289431799278 19.68882143583277 42-43 20.68567175710319 29.435176565467874 30.738457285780974 19.140694391647965 44-45 20.145746160994367 28.904295681728335 31.162714283513964 19.78724387376333 46-47 21.093128434047596 28.886067557781953 30.245023159605143 19.7757808485653 48-49 20.714078723804956 30.131550005452755 30.02912859237407 19.125242678368217 50-51 21.342767025643152 29.393101227426026 29.654395966166202 19.609735780764616 52-53 20.220808171873863 28.999945578343056 29.445792435800417 21.33345381398267 54-55 21.417254783079827 29.117605619628446 29.80755130383776 19.65758829345397 56-57 20.708126341008327 29.00340923702352 29.462232809931855 20.826231612036302 58-59 20.597822458807784 28.800318794824104 30.20934261919116 20.39251612717695 60-61 20.800653124935817 29.18618168374787 30.369508513216537 19.643656678099777 62-63 20.224246999457023 29.311224343108833 29.914002643418602 20.550526014015542 64-65 21.179936386188338 29.01627397892767 29.760139863299727 20.04364977158427 66-67 20.851663866285083 30.14132288807095 28.941846082482208 20.065167163161757 68-69 21.07085080283719 30.276077727686896 28.733359296240884 19.919712173235034 70-71 20.558639156264395 31.166636467512486 28.769398847830363 19.505325528392753 72-73 20.221953480690154 31.42564735062368 28.685072608488532 19.667326560197633 74-75 20.930414572549477 30.509087711960674 28.60222736145137 19.958270354038472 76-77 21.269488825800494 29.593000406566116 29.129486274233468 20.008024493399926 78-79 21.126597982188045 29.467387196424465 29.675987544776365 19.73002727661113 80-81 20.31947578597086 30.0571120373164 29.45423879268435 20.169173384028387 82-83 19.58430855034543 29.95002083406494 29.648301679724664 20.817368935864966 84-85 19.704696448273513 29.476257333194223 30.09068063050888 20.728365588023387 86-87 19.713659871424035 29.66962903258274 30.718083085100133 19.898628010893095 88-89 19.930996688481905 30.186555836319563 30.230779248148593 19.65166822704994 90-91 20.371888909065063 29.609952533036658 29.681949982622015 20.33620857527626 92-93 19.686871905561098 30.43336680088614 29.621722146993847 20.25803914655891 94-95 19.32970260542251 30.951229622861426 29.55065633705951 20.16841143465655 96-97 19.83233785346109 31.377090076343407 29.024709100485534 19.765862969709968 98-99 20.3097262684912 30.42303960450755 28.716364950418612 20.550869176582637 100-101 20.233708311963543 30.30723408398209 28.328937506688224 21.130120097366138 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 149.0 1 95.0 2 73.0 3 154.0 4 160.0 5 105.0 6 79.5 7 72.0 8 72.5 9 75.0 10 79.5 11 83.0 12 84.0 13 77.5 14 80.0 15 101.0 16 121.5 17 126.0 18 164.0 19 244.5 20 334.0 21 489.5 22 757.5 23 1116.0 24 1639.5 25 2416.0 26 3584.0 27 5295.5 28 7185.0 29 9148.5 30 11423.0 31 13980.0 32 19700.0 33 30681.5 34 46960.0 35 70449.5 36 104253.5 37 146378.0 38 188396.0 39 206807.5 40 203327.0 41 183866.0 42 157404.5 43 147502.0 44 138862.5 45 128271.5 46 125861.0 47 112250.0 48 83697.5 49 62705.5 50 50203.5 51 40991.5 52 34024.5 53 26598.5 54 19434.5 55 13827.5 56 9665.0 57 6417.0 58 4565.5 59 3352.0 60 2482.5 61 1844.0 62 1309.5 63 899.5 64 624.5 65 445.5 66 303.5 67 222.0 68 172.0 69 126.5 70 85.5 71 59.5 72 47.5 73 36.0 74 32.5 75 26.0 76 24.0 77 18.5 78 9.5 79 5.5 80 6.0 81 5.5 82 2.0 83 2.5 84 3.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1645761619623294 2 0.0 3 1.6428865681290686E-4 4 0.02057715426581658 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.696494778290404E-4 12-13 0.0 14-15 0.0 16-17 0.0011089484334871212 18-19 0.0 20-21 0.0 22-23 5.339381346419473E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.446597766742145E-4 38-39 7.803711198613075E-4 40-41 0.005092948361200112 42-43 0.0031009483973436164 44-45 0.0010884123513855077 46-47 0.004600082390761391 48-49 0.007783175116511462 50-51 0.005421525674825926 52-53 0.0019098556354500422 54-55 0.011171628663277665 56-57 0.019303917175516556 58-59 0.023472741842144065 60-61 0.01186985545473252 62-63 5.339381346419473E-4 64-65 0.014868123441568069 66-67 0.03468544266962496 68-69 0.020412865609003676 70-71 0.03680065912609113 72-73 0.031235380876553916 74-75 0.030311257181981313 76-77 0.038628370433134725 78-79 0.017209236801151995 80-81 0.012876123477711575 82-83 0.0010062680229790545 84-85 0.010719834857042171 86-87 0.022014680012929517 88-89 0.02086465941523917 90-91 0.025670102627016696 92-93 0.04265344252505094 94-95 0.028750514942258696 96-97 0.03027018501777809 98-99 0.037478349835444374 100-101 0.017352989375863284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2434739.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.856285807152574 #Duplication Level Percentage of deduplicated Percentage of total 1 87.5681355514274 34.90140638137169 2 5.3798355333378876 4.288405252243799 3 1.8603874381501042 2.2244440034084083 4 0.997775713013642 1.5907053595722864 5 0.7285342660743364 1.4518334964481443 6 0.4977469779159812 1.1903007486879484 7 0.39828315283130633 1.1111861019992861 8 0.25630219406992866 0.8172202799881092 9 0.219729705094802 0.78818489339218 >10 1.6309698315007706 13.021143063651945 >50 0.22441293840111076 6.34436814905641 >100 0.1980291039351295 17.367064250923114 >500 0.031923791284252954 8.591837559622567 >1k 0.007726307664856766 4.7282383579907385 >5k 1.03747649267523E-4 0.20737004744548504 >10k+ 1.0374764915669978E-4 1.3762920541979455 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 33284 1.3670459133401978 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5015 0.20597690347918196 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4076 0.16741014129235207 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3978 0.16338506920043586 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 3840 0.15771711054039056 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3256 0.13373096664570616 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3143 0.12908981209074155 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2986 0.12264148231083496 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2745 0.11274309073785733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.446597766742143E-4 0.0 0.0 2 9.857319408774412E-4 0.0 0.0 3 0.0010268041050806678 0.0 0.0 4 0.0010678762692838946 0.0 0.0 5 0.0013143092545032547 0.0 0.0 6 0.0013553814187064815 0.0 0.0 7 0.001560742239722615 0.0 0.0 8 0.0018893195533484287 0.0 0.0 9 0.0019303917175516555 0.0 0.0 10-11 0.0031009483973436164 0.0 0.0 12-13 0.003963463845611378 0.0 0.0 14-15 0.005113484443301726 0.0 0.0 16-17 0.006284041123093687 0.0 0.0 18-19 0.008234968922746956 0.0 0.0 20-21 0.010452865789721197 0.0 0.0 22-23 0.01310202038082932 0.0 0.0 24-25 0.01651101000969714 0.0 0.0 26-27 0.019919999638564955 0.0 0.0 28-29 0.023801319155769878 0.0 0.0 30-31 0.037704246738562125 0.0 0.0 32-33 0.04308470024918482 0.0 0.0 34-35 0.04710977234110104 0.0 0.0 36-37 0.05134020525403339 0.0 0.0 38-39 0.05633047320472544 0.0 0.0 40-41 0.062450225671006214 0.0 0.0 42-43 0.0687342667940999 0.0 0.0 44-45 0.07569599862654683 0.0 0.0 46-47 0.08237022530957117 0.0 0.0 48-49 0.08894177158208744 0.0 0.0 50-51 0.09621154464605858 0.0 0.0 52-53 0.10341970946372486 0.0 2.0536082101613354E-5 54-55 0.11222968868531699 0.0 4.107216420322671E-5 56-57 0.12130663697423008 0.0 4.107216420322671E-5 58-59 0.13178003884605288 0.0 4.107216420322671E-5 60-61 0.14305434791983862 0.0 4.107216420322671E-5 62-63 0.15504741986718085 0.0 6.160824630484006E-5 64-65 0.166670842336694 0.0 8.214432840645342E-5 66-67 0.1780683679030894 0.0 1.0268041050806679E-4 68-69 0.19310078000147038 0.0 1.2321649260968015E-4 70-71 0.21010465598160624 0.0 1.2321649260968015E-4 72-73 0.22811479998472114 0.0 1.2321649260968015E-4 74-75 0.24766515014545706 0.0 1.2321649260968015E-4 76-77 0.2676878301945301 0.0 1.2321649260968015E-4 78-79 0.29087306688725156 0.0 1.2321649260968015E-4 80-81 0.3162967365290489 0.0 1.2321649260968015E-4 82-83 0.34514993188181564 0.0 1.2321649260968015E-4 84-85 0.3783157044759212 0.0 1.2321649260968015E-4 86-87 0.41523958009462203 0.0 1.2321649260968015E-4 88-89 0.45510011545385354 0.0 1.437525747112935E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGACG 15 6.577498E-4 95.1498 1 CGCGTGG 15 6.577498E-4 95.1498 1 TCGGAAG 5220 0.0 68.424416 3 CGGAAGA 5215 0.0 68.39894 4 GCCGAAC 35 3.3863344E-6 67.96414 1 GATCGGA 5395 0.0 66.31386 1 ATCGGAA 5545 0.0 64.41396 2 GAAGAGC 5600 0.0 63.606476 6 GGAAGAG 6165 0.0 58.162354 5 CGTCCCG 25 0.005023316 56.99608 4 TTGTGTT 27030 0.0 49.176575 1 CGATCGG 50 2.8017217E-5 47.5749 1 CGACGGG 75 1.3468525E-7 44.40324 1 CGAGTCC 55 4.919002E-5 43.249912 1 GCCGTCT 4140 0.0 43.19804 50-51 TCGCCGG 55 4.9669696E-5 43.17885 2 ATGCCGT 4155 0.0 42.754536 48-49 TCGTATG 4155 0.0 42.696495 44-45 TATGCCG 4110 0.0 42.644806 48-49 TGCCGTC 4130 0.0 42.612556 50-51 >>END_MODULE