##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_35005-1.3410000003578e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2665757 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.680168522487236 33.0 33.0 33.0 33.0 33.0 2 32.722207988199976 33.0 33.0 33.0 33.0 33.0 3 32.749336867538936 33.0 33.0 33.0 33.0 33.0 4 36.68120462592802 37.0 37.0 37.0 37.0 37.0 5 36.67953568160939 37.0 37.0 37.0 37.0 37.0 6 36.57934575432044 37.0 37.0 37.0 37.0 37.0 7 36.53836640023828 37.0 37.0 37.0 37.0 37.0 8 36.477743470241286 37.0 37.0 37.0 37.0 37.0 9 36.48244119775358 37.0 37.0 37.0 37.0 37.0 10-11 36.43949767364393 37.0 37.0 37.0 37.0 37.0 12-13 36.41736080970621 37.0 37.0 37.0 37.0 37.0 14-15 38.305544353817695 40.0 37.0 40.0 37.0 40.0 16-17 38.29851914484328 40.0 37.0 40.0 37.0 40.0 18-19 38.31388476143925 40.0 37.0 40.0 37.0 40.0 20-21 38.25904743005458 40.0 37.0 40.0 37.0 40.0 22-23 38.171772783490766 40.0 37.0 40.0 37.0 40.0 24-25 38.14547500015943 40.0 37.0 40.0 37.0 40.0 26-27 38.05021800561717 40.0 37.0 40.0 37.0 40.0 28-29 37.95811283624126 40.0 37.0 40.0 33.0 40.0 30-31 37.906850661932054 40.0 37.0 40.0 33.0 40.0 32-33 37.79453903712904 40.0 37.0 40.0 33.0 40.0 34-35 37.72199922948716 40.0 37.0 40.0 33.0 40.0 36-37 37.64645089556175 40.0 37.0 40.0 33.0 40.0 38-39 37.55006889225087 40.0 37.0 40.0 33.0 40.0 40-41 37.47139274134889 40.0 37.0 40.0 33.0 40.0 42-43 37.32435458295711 40.0 37.0 40.0 33.0 40.0 44-45 37.24113750803242 40.0 37.0 40.0 33.0 40.0 46-47 37.07327449576237 40.0 37.0 40.0 33.0 40.0 48-49 36.98942758098356 37.0 37.0 40.0 33.0 40.0 50-51 36.837015339357635 37.0 37.0 40.0 33.0 40.0 52-53 36.69070324114313 37.0 37.0 40.0 33.0 40.0 54-55 36.555472235466326 37.0 37.0 40.0 33.0 40.0 56-57 36.34685532102138 37.0 37.0 40.0 33.0 40.0 58-59 36.06019397116842 37.0 37.0 40.0 33.0 40.0 60-61 35.83463140113671 37.0 37.0 40.0 33.0 40.0 62-63 35.567523784050834 37.0 35.0 40.0 27.0 40.0 64-65 35.28645709267574 37.0 33.0 37.0 27.0 40.0 66-67 34.99800638242721 37.0 33.0 37.0 27.0 40.0 68-69 34.682687694339734 37.0 33.0 37.0 27.0 40.0 70-71 34.3663118956454 37.0 33.0 37.0 27.0 40.0 72-73 34.05456798950542 37.0 33.0 37.0 27.0 37.0 74-75 33.67943739808242 37.0 33.0 37.0 27.0 37.0 76-77 30.901025487319362 33.0 30.0 35.0 24.5 37.0 78-79 33.373764375372545 35.0 33.0 37.0 27.0 37.0 80-81 33.77224518213775 37.0 33.0 37.0 27.0 37.0 82-83 33.667312136852686 37.0 33.0 37.0 27.0 37.0 84-85 33.545463071090126 37.0 33.0 37.0 27.0 37.0 86-87 33.450993282583525 37.0 33.0 37.0 27.0 37.0 88-89 33.18303750116759 37.0 33.0 37.0 27.0 37.0 90-91 33.18500786080652 37.0 33.0 37.0 27.0 37.0 92-93 33.04282404585264 37.0 33.0 37.0 27.0 37.0 94-95 32.861915771017394 35.0 33.0 37.0 27.0 37.0 96-97 32.74729204499885 33.0 33.0 37.0 27.0 37.0 98-99 32.6277955942721 33.0 33.0 37.0 27.0 37.0 100-101 30.997275820714343 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.12633035389128366 2306 2 0.11687878890558778 2306 3 0.09011215857783128 2306 4 0.1481898777187851 2306 5 0.1866570366185556 2306 6 0.17512995759839356 2306 7 0.1838413857687442 2306 8 0.22626545668144615 2306 9 0.15900406154245417 2306 10-11 0.15997508626142576 2306 12-13 0.20214625526296714 2306 14-15 0.4409550114858618 2306 16-17 0.38944926665261903 2306 18-19 0.38400987974085155 2306 20-21 0.33414912099729577 2306 22-23 0.3070543679528228 2306 24-25 0.28576911698727514 2306 26-27 0.2842399239991309 2306 28-29 0.30713461343898274 2306 30-31 0.35176901910928393 2306 32-33 0.3481921970099293 2306 34-35 0.4098319820945875 2306 36-37 0.3843024049031527 2306 38-39 0.3668921895617743 2306 40-41 0.45222294579226485 2306 42-43 0.4086936616434187 2306 44-45 0.32420321684936937 2306 46-47 0.5147579550440895 2306 48-49 0.49406517633322267 2306 50-51 0.44645134946803466 2306 52-53 0.3067586058233971 2306 54-55 0.42667886995780435 2306 56-57 0.4200700250626994 2306 58-59 0.478408820049367 2306 60-61 0.4522187674346725 2306 62-63 0.5028102709210245 2306 64-65 0.43910738870231825 2306 66-67 0.34146235443840567 2306 68-69 0.3439338048341156 2306 70-71 0.2856238858689508 2306 72-73 0.29454535984391583 2306 74-75 0.5274824078919025 2306 76-77 0.04331292941269638 2306 78-79 0.19843312131276747 2306 80-81 0.3646322530926156 2306 82-83 0.37535108674367024 2306 84-85 0.5175603102630717 2306 86-87 0.4505067511490921 2306 88-89 0.7217272555988572 2306 90-91 0.5836375929052906 2306 92-93 0.48557754251786633 2306 94-95 0.3618707502809997 2306 96-97 0.43634134037334604 2306 98-99 0.36532782798776964 2306 100-101 0.30946192904532666 2307 1 0.16243126402795838 2307 2 0.12099983792016644 2307 3 0.10331365058077324 2307 4 0.13366507470427536 2307 5 0.15924413289478423 2307 6 0.11489140823740485 2307 7 0.18484200604171974 2307 8 0.19154644405931265 2307 9 0.15524522590735756 2307 10-11 0.17727344872216122 2307 12-13 0.23569051550607156 2307 14-15 0.4063661910925447 2307 16-17 0.36009812612219605 2307 18-19 0.26678037966970436 2307 20-21 0.29297885095071763 2307 22-23 0.3482283577773799 2307 24-25 0.4128067416114618 2307 26-27 0.4619179084002383 2307 28-29 0.4364873348750393 2307 30-31 0.4211900728665867 2307 32-33 0.40998886836661796 2307 34-35 0.4360761780785367 2307 36-37 0.4461255796778545 2307 38-39 0.5837784936182899 2307 40-41 0.49508920210542584 2307 42-43 0.3942420917576257 2307 44-45 0.28035470666694096 2307 46-47 0.4625341322840626 2307 48-49 0.33969392575316704 2307 50-51 0.4860776791724106 2307 52-53 0.3846833044981324 2307 54-55 0.2871379091577424 2307 56-57 0.2392734718089642 2307 58-59 0.4254133758474268 2307 60-61 0.515287602855885 2307 62-63 0.64600591357312 2307 64-65 0.554524659445434 2307 66-67 0.40552274317380466 2307 68-69 0.42385090958213567 2307 70-71 0.29955422186719005 2307 72-73 0.35319021683239527 2307 74-75 0.4393531958245731 2307 76-77 -0.09870796086036293 2307 78-79 0.3863986166523503 2307 80-81 0.41482368216620813 2307 82-83 0.5949563160075328 2307 84-85 0.5930455320501906 2307 86-87 0.4555491426687368 2307 88-89 0.7600344590418899 2307 90-91 0.6445859223995001 2307 92-93 0.5562777349233841 2307 94-95 0.5632518573427205 2307 96-97 0.48734321607138753 2307 98-99 0.6316485863017078 2307 100-101 0.3917117995505528 2201 1 0.09943337314942369 2201 2 0.10324936802326334 2201 3 0.07126331032137756 2201 4 0.10352692121936258 2201 5 0.10886278962153284 2201 6 0.13270841616078854 2201 7 0.09700354965248437 2201 8 0.1546727667258736 2201 9 0.1775865963495562 2201 10-11 0.08762942269820684 2201 12-13 0.1446286489849271 2201 14-15 0.2579876200882083 2201 16-17 0.3252754053063711 2201 18-19 0.23478105934001547 2201 20-21 0.278035928520211 2201 22-23 0.3074436734913846 2201 24-25 0.31873257805920474 2201 26-27 0.23409405018607288 2201 28-29 0.25023411347444124 2201 30-31 0.2270707393721949 2201 32-33 0.26596831481489147 2201 34-35 0.3288635453206581 2201 36-37 0.28219999687842545 2201 38-39 0.42395069313769085 2201 40-41 0.16670082009141396 2201 42-43 0.27104213516363984 2201 44-45 0.26807297037277067 2201 46-47 0.3382751493155638 2201 48-49 0.3442715410213637 2201 50-51 0.23870302855287662 2201 52-53 0.3730687347245265 2201 54-55 0.3745905343061722 2201 56-57 0.3968586282023523 2201 58-59 0.3285461781566994 2201 60-61 0.2808698932019311 2201 62-63 0.3662635273765886 2201 64-65 0.36195780547495815 2201 66-67 0.3477705272119138 2201 68-69 0.22953196200828785 2201 70-71 0.27036010454323645 2201 72-73 0.3334333351705041 2201 74-75 0.256664782685057 2201 76-77 0.03789404930254037 2201 78-79 0.2742203045657945 2201 80-81 0.3200437243129173 2201 82-83 0.35718044555394357 2201 84-85 0.46736982499434276 2201 86-87 0.36182809954897266 2201 88-89 0.5751322051890426 2201 90-91 0.46879223315173135 2201 92-93 0.4738625742563727 2201 94-95 0.4963681903740209 2201 96-97 0.5959272466770926 2201 98-99 0.5010275185762652 2201 100-101 0.27297156534563527 1101 1 0.11727921682061293 1101 2 0.09450131314962817 1101 3 0.12067013375506974 1101 4 0.10645126163632312 1101 5 0.10630353131120529 1101 6 0.11741303117516111 1101 7 0.13914822694363949 1101 8 0.11318867521959675 1101 9 0.1272605190913083 1101 10-11 0.14240145920969383 1101 12-13 0.21491155896763559 1101 14-15 0.1449205252004262 1101 16-17 0.30528357872528744 1101 18-19 0.24610863212519263 1101 20-21 0.22753713304559398 1101 22-23 0.27148820204840973 1101 24-25 0.2938810052183669 1101 26-27 0.27765239742827674 1101 28-29 0.3135525297384447 1101 30-31 0.31396212253404343 1101 32-33 0.344719021854516 1101 34-35 0.27850718098181204 1101 36-37 0.32030329301322524 1101 38-39 0.28134213237157724 1101 40-41 0.3405077342226335 1101 42-43 0.27567084011263177 1101 44-45 0.2055895975707358 1101 46-47 0.37868364017582934 1101 48-49 0.25369567311233254 1101 50-51 0.3632526105502336 1101 52-53 0.30327839908637344 1101 54-55 0.4593450411465554 1101 56-57 0.409263336451815 1101 58-59 0.37394231679949286 1101 60-61 0.20738463605233193 1101 62-63 0.28911496597199005 1101 64-65 0.4494092949854007 1101 66-67 0.21601957787564885 1101 68-69 0.3408823114966424 1101 70-71 0.2803433811633269 1101 72-73 0.1993545781195465 1101 74-75 0.10414774210936883 1101 76-77 -0.35950805589513024 1101 78-79 0.061177500735205115 1101 80-81 0.29176418601899456 1101 82-83 0.35473108023199273 1101 84-85 0.2405008665506685 1101 86-87 0.1963683457583798 1101 88-89 0.4680015827404276 1101 90-91 0.2263693240039757 1101 92-93 0.20394032610368384 1101 94-95 0.38637155060283135 1101 96-97 0.32670896850580533 1101 98-99 0.18871410131771427 1101 100-101 0.16704597988408665 2304 1 0.1456996791794225 2304 2 0.14072223055006816 2304 3 0.11749075293190714 2304 4 0.09253253916458704 2304 5 0.1435249941331378 2304 6 0.14272724707378615 2304 7 0.13011379506109932 2304 8 0.1022295563932758 2304 9 0.10816066655711865 2304 10-11 0.13874651798278137 2304 12-13 0.1833411752797005 2304 14-15 0.26491016668118306 2304 16-17 0.3573168075382043 2304 18-19 0.31643340106924 2304 20-21 0.3446074789505573 2304 22-23 0.35588058081183505 2304 24-25 0.3264183149661477 2304 26-27 0.3304406309883845 2304 28-29 0.3815394306445583 2304 30-31 0.36712369672969913 2304 32-33 0.24670494713700464 2304 34-35 0.3488029580428389 2304 36-37 0.3188032903945981 2304 38-39 0.4284418966208037 2304 40-41 0.4009290629711302 2304 42-43 0.412535815000048 2304 44-45 0.41637339967475384 2304 46-47 0.5368336138773842 2304 48-49 0.5397532521142736 2304 50-51 0.356529579922082 2304 52-53 0.40985415094877453 2304 54-55 0.526532327214511 2304 56-57 0.41331817862000264 2304 58-59 0.5420543691180626 2304 60-61 0.5129214871672332 2304 62-63 0.5073273302058041 2304 64-65 0.3839463713017608 2304 66-67 0.5313179960036933 2304 68-69 0.4784934065219346 2304 70-71 0.4047941252633862 2304 72-73 0.4301016297385658 2304 74-75 0.3479224366471527 2304 76-77 0.10798848939467476 2304 78-79 0.5018700214279335 2304 80-81 0.4991878288808991 2304 82-83 0.6338688578900644 2304 84-85 0.5757436427086446 2304 86-87 0.5127369384587084 2304 88-89 0.7256976183716333 2304 90-91 0.5506300683958187 2304 92-93 0.5476683717664628 2304 94-95 0.6672089072613474 2304 96-97 0.5985008576617972 2304 98-99 0.6048347853615468 2304 100-101 0.7346342891597644 2202 1 0.10198205021777085 2202 2 0.09919403207416622 2202 3 0.05872589005078055 2202 4 0.09499869171799702 2202 5 0.10748237344828482 2202 6 0.013919468269016022 2202 7 0.09515080192274183 2202 8 0.11162850543234981 2202 9 0.1260292996992689 2202 10-11 0.10292926976256922 2202 12-13 0.023183820893542872 2202 14-15 0.13287490673474878 2202 16-17 0.17923904177914807 2202 18-19 0.06565925651161564 2202 20-21 0.2514622137525677 2202 22-23 0.1794994093653699 2202 24-25 0.07484657991363974 2202 26-27 0.08578831923610863 2202 28-29 0.15870589954239023 2202 30-31 0.20180737888886568 2202 32-33 0.12514560628417115 2202 34-35 0.14768790870006399 2202 36-37 0.13914337392900222 2202 38-39 0.26296638643807313 2202 40-41 0.23770427704068453 2202 42-43 0.22883805379129996 2202 44-45 0.2428244288699659 2202 46-47 0.42311353145055364 2202 48-49 0.3122830833189525 2202 50-51 0.16139765944864592 2202 52-53 0.15274360254016273 2202 54-55 0.22450896425957723 2202 56-57 0.24193172047901612 2202 58-59 0.43030811742477226 2202 60-61 0.19787975682791625 2202 62-63 0.20285847455442507 2202 64-65 0.061542273757751786 2202 66-67 2.8766236157906633E-4 2202 68-69 0.16361901245154087 2202 70-71 0.07440769335441644 2202 72-73 0.1308952940033734 2202 74-75 0.014324554273919432 2202 76-77 -0.1476707254930396 2202 78-79 0.15778044646034317 2202 80-81 0.033202097383941975 2202 82-83 0.16888260055259963 2202 84-85 0.28244611179301415 2202 86-87 0.19894208620926435 2202 88-89 0.5193446523448415 2202 90-91 0.27669519546594046 2202 92-93 0.3303525655356907 2202 94-95 0.2875251419484073 2202 96-97 0.2124547993824848 2202 98-99 0.20704295545201035 2202 100-101 0.3403440906931223 1102 1 0.06332007832087783 1102 2 0.048685274302883386 1102 3 -0.001807503095427876 1102 4 0.0289776338849137 1102 5 0.08054864313716337 1102 6 0.05187345000008747 1102 7 0.013388778334302742 1102 8 0.04790876659166088 1102 9 0.025301437767176083 1102 10-11 0.029583266748431924 1102 12-13 0.05423329971981872 1102 14-15 0.12803245053452628 1102 16-17 0.06234867996013804 1102 18-19 0.03161536455288427 1102 20-21 0.0726235703110163 1102 22-23 0.11079581906441405 1102 24-25 0.025445275364944564 1102 26-27 0.04706691921558104 1102 28-29 0.027627190190209205 1102 30-31 0.009953976371413376 1102 32-33 0.08702652043533732 1102 34-35 -0.008454806768995127 1102 36-37 0.050788884079672414 1102 38-39 0.1431139771969825 1102 40-41 0.047915650866038106 1102 42-43 -0.007067810189106183 1102 44-45 -0.05740270822619209 1102 46-47 0.1283613467261091 1102 48-49 0.056200747447419985 1102 50-51 0.0342946291758679 1102 52-53 -0.0541618830344035 1102 54-55 -0.01791948724317649 1102 56-57 0.04191295616596591 1102 58-59 0.20192388855692656 1102 60-61 0.08532016102650886 1102 62-63 0.05669578437273515 1102 64-65 0.018945723334155673 1102 66-67 0.06828240461042157 1102 68-69 -0.053114964494092476 1102 70-71 -0.1424920116262811 1102 72-73 -0.21582694081850917 1102 74-75 -0.14111297865721895 1102 76-77 -0.17946173004046173 1102 78-79 0.024374447557811152 1102 80-81 0.041750232448315217 1102 82-83 0.11447747918976603 1102 84-85 0.14350159894296866 1102 86-87 0.16799937523240516 1102 88-89 0.32372489500500734 1102 90-91 -0.027131320582924445 1102 92-93 0.0962461570626445 1102 94-95 0.3174948538826641 1102 96-97 0.15959777284135868 1102 98-99 0.08646539831912037 1102 100-101 0.151994841749179 2305 1 0.10665932373115083 2305 2 0.1051434031017564 2305 3 0.07929518173946093 2305 4 0.06429615518278098 2305 5 0.03953145130849123 2305 6 0.044831901642105265 2305 7 -0.011258367352546372 2305 8 0.066021647745103 2305 9 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-0.8131336039932648 1213 94-95 -0.8193628620416362 1213 96-97 -0.7323791842892646 1213 98-99 -0.8242995510758249 1213 100-101 -0.8354283273122292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 20.0 10 31.0 11 23.0 12 38.0 13 95.0 14 260.0 15 654.0 16 1289.0 17 2077.0 18 2873.0 19 3660.0 20 4749.0 21 5925.0 22 7479.0 23 9439.0 24 11912.0 25 14848.0 26 18661.0 27 23972.0 28 31825.0 29 42595.0 30 48216.0 31 56790.0 32 76255.0 33 107191.0 34 162982.0 35 284003.0 36 576987.0 37 937641.0 38 232752.0 39 510.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.402453248201624 26.076396090915377 21.856146347182268 14.665004313700736 2 32.599820613806884 22.63769728448617 31.186938644445085 13.575543457261857 3 37.72950371977588 18.847139568872123 26.27337426737371 17.149982443978285 4 31.805470768798987 18.66393414636068 31.667919238566355 17.86267584627397 5 31.258250470691817 17.216648029058913 33.91295605713499 17.612145443114283 6 27.02451873895483 17.91768717103622 37.25785958735173 17.79993450265722 7 33.85083486604368 18.772491266083144 27.70320025418671 19.67347361368647 8 31.01242911488181 26.708435915201573 23.4161628385483 18.862972131368313 9 29.077068915133676 25.55037837282243 25.634069421931553 19.73848329011234 10-11 24.08405844454852 28.233730927672816 30.547834644820078 17.13437598295858 12-13 23.786357871328857 27.065013802833494 29.995588495125396 19.153039830712252 14-15 22.7824028971883 27.768510032985 30.74076144224699 18.708325627579708 16-17 22.449931969675767 27.985522126642564 31.344366987867833 18.220178915813833 18-19 21.57655405200099 27.921656024911496 31.652097321698864 18.84969260138865 20-21 22.081776395973076 28.13127753204812 31.17988623869317 18.60705983328563 22-23 21.419993018838447 28.668565697663183 30.91643594634221 18.995005337156154 24-25 21.71319441344429 27.436859398662367 31.385775222572796 19.464170965320545 26-27 21.54196725357938 27.688176379167345 30.996936329905537 19.772920037347742 28-29 21.429803991886732 28.70310384629957 30.46059712119297 19.40649504062073 30-31 20.565434133718867 29.002005809231672 30.663878965712176 19.76868109133728 32-33 20.332892307888528 29.61712189070497 30.91099451300325 19.138991288403258 34-35 21.0864868778362 28.94995680401477 31.052942935158757 18.91061338299027 36-37 20.451868379867143 29.189768513864113 31.76054457087209 18.597818535396655 38-39 21.08951980955339 29.24867972573408 30.397950433164 19.263850031548536 40-41 21.024685073148113 28.570005690985845 30.83632732209667 19.568981913769374 42-43 20.91773209652061 29.08048598681982 30.78711197826887 19.2146699383907 44-45 20.506844849453827 28.52415119777832 31.375455926282026 19.593548026485834 46-47 21.162216203238245 28.44335694242283 30.671677345778125 19.722749508560796 48-49 20.756325203855944 29.913884451087235 30.05237387530099 19.277416469755828 50-51 21.482922327492904 29.142374273854248 29.59311453454456 19.781588864108283 52-53 20.878690918066837 28.908373755563936 29.445415311573946 20.767520014795277 54-55 21.765886563670062 28.961079888649614 29.68680828074704 19.586225266933287 56-57 21.202127298346483 29.174918680495722 29.529949909173915 20.093004111983884 58-59 21.02507781531407 29.321050480005717 29.965410621530065 19.68846108315015 60-61 21.046206277777912 29.531698124462686 29.940259607244478 19.48183599051492 62-63 20.620567129790278 29.33149119249859 29.698950272325987 20.348991405385146 64-65 21.086655682577714 29.006267608949678 29.713730229999918 20.193346478472694 66-67 21.207827906147685 29.165517435478876 29.463341870479297 20.16331278789414 68-69 21.276540262443675 29.653824009617217 29.251511929880447 19.81812379805866 70-71 20.560392738746323 30.734238625864705 29.021804475726647 19.68356415966232 72-73 20.40510275320001 30.493346319175924 28.98206291404334 20.119488013580725 74-75 20.886726088435456 29.7289584592342 29.066096852092176 20.318218600238165 76-77 20.786711131517624 29.26463560143977 29.350798290181 20.5978549768616 78-79 20.631252688483695 29.34033591296451 29.534591463973992 20.493819934577807 80-81 20.069561739655033 29.72112649423989 29.48840362541936 20.72090814068572 82-83 19.545749705615677 29.523249327670854 29.637026663960718 21.29397430275275 84-85 19.715075227665757 29.297493073937463 29.965947914359 21.02148378403778 86-87 20.01443054773609 29.348923777929404 30.53559807134942 20.10104760298509 88-89 20.188845327381053 29.948349033861287 29.83933270223552 20.02347293652214 90-91 20.447630677768537 29.63233888408081 29.399793964638537 20.52023647351211 92-93 19.887296954567134 30.08767126783514 29.600020715953875 20.42501106164385 94-95 19.604500249154974 30.463904798784846 29.595033110594787 20.336561841465393 96-97 19.8669894212931 30.95056015513836 29.22547895365662 19.956971469911924 98-99 20.4117382496199 30.217648728757823 28.81837513141351 20.55223789020876 100-101 20.44017736009893 30.07049456123711 28.238682750837423 21.250645327826536 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 366.0 1 233.5 2 109.0 3 174.0 4 198.5 5 149.0 6 136.0 7 139.0 8 155.0 9 159.0 10 149.0 11 156.0 12 154.0 13 156.0 14 152.0 15 159.0 16 196.5 17 212.0 18 224.0 19 275.5 20 373.5 21 510.0 22 722.5 23 1104.0 24 1659.0 25 2528.0 26 4283.0 27 7086.5 28 10081.5 29 13075.5 30 16452.5 31 19818.0 32 25668.0 33 37056.0 34 54253.0 35 78885.5 36 114645.5 37 154950.0 38 188847.0 39 206363.5 40 206265.0 41 191511.0 42 172274.0 43 163120.5 44 150492.0 45 134770.5 46 126006.0 47 112359.5 48 93818.0 49 78055.5 50 64157.0 51 52527.5 52 42688.5 53 34194.5 54 27974.5 55 21977.5 56 15600.0 57 10314.5 58 7037.5 59 5040.0 60 3707.0 61 2782.0 62 1995.0 63 1385.5 64 1017.5 65 748.5 66 518.0 67 395.0 68 331.0 69 263.0 70 184.0 71 124.0 72 85.0 73 55.5 74 42.0 75 30.5 76 20.0 77 15.0 78 10.0 79 7.5 80 5.5 81 3.5 82 3.0 83 3.0 84 2.0 85 1.5 86 0.5 87 1.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16764468779412378 2 0.0 3 1.8756398276362026E-4 4 0.021532345221263603 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.189611431199468E-4 12-13 0.0 14-15 0.0 16-17 0.0012754350827926177 18-19 0.0 20-21 0.0 22-23 4.313971603563265E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.378199138181012E-4 38-39 8.62794320712653E-4 40-41 0.005176765924275919 42-43 0.003301126096639716 44-45 0.0011253838965817216 46-47 0.004388997196668714 48-49 0.007896443674348412 50-51 0.005326817110486814 52-53 0.0015567810569380482 54-55 0.011141300576159042 56-57 0.018700129081532936 58-59 0.023726843819597963 60-61 0.011535184939962645 62-63 6.752303379490329E-4 64-65 0.014498695867627844 66-67 0.03496192638713881 68-69 0.020163128147089177 70-71 0.036875079011327735 72-73 0.030141532030113775 74-75 0.029878942454244703 76-77 0.03865693684758213 78-79 0.017012053236660354 80-81 0.013317042776217036 82-83 9.00307117265377E-4 84-85 0.01055985222959182 86-87 0.02166364000919814 88-89 0.02100716606952547 90-91 0.02552745805412871 92-93 0.04291463925631631 94-95 0.028097084617990313 96-97 0.029297494107677482 98-99 0.036818809816498654 100-101 0.0166931944659622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2665757.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.663498626922 #Duplication Level Percentage of deduplicated Percentage of total 1 87.455028062081 40.80957581892341 2 5.460292607048684 5.095927131432173 3 1.9824074174213862 2.7751819740252857 4 1.0108239132884227 1.8867432115917693 5 0.7333373055081465 1.7110042174325035 6 0.40409699250360565 1.131394767290119 7 0.33321298486333123 1.0884218563149848 8 0.29807513082531184 1.1127382766389229 9 0.22250922269924003 0.9344752927113122 >10 1.7528681149246688 15.634007919225063 >50 0.18321083354077605 6.047195694493722 >100 0.14674059267657036 14.531590873612659 >500 0.014705495238386056 4.368934282323289 >1k 0.002449856988928408 1.6686132094469799 >5k 1.6098026213215015E-4 0.3956518910314723 >10k+ 8.049012965246454E-5 0.8085435835062089 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 21527 0.8075379713904907 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5273 0.1978049762225139 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5261 0.1973548226638812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.1264076207996454E-4 0.0 0.0 2 4.8766635518541265E-4 0.0 0.0 3 5.251791517381367E-4 0.0 0.0 4 5.626919482908607E-4 0.0 0.0 5 5.626919482908607E-4 0.0 0.0 6 6.002047448435847E-4 0.0 0.0 7 6.752303379490329E-4 0.0 0.0 8 9.753327103708253E-4 0.0 0.0 9 0.0012379222862398936 0.0 0.0 10-11 0.0019131526241889265 0.0 0.0 12-13 0.002757190546625218 0.0 0.0 14-15 0.004313971603563265 0.0 0.0 16-17 0.005833239863948589 0.0 0.0 18-19 0.007577584903650257 0.0 0.0 20-21 0.009772083501984614 0.0 0.0 22-23 0.012060364091700781 0.0 0.0 24-25 0.015155169807300516 0.0 0.0 26-27 0.018868936666020197 0.0 0.0 28-29 0.022470165135081703 0.0 0.0 30-31 0.033630222109517105 0.0 0.0 32-33 0.038450616466542145 0.0 0.0 34-35 0.04265204968044724 0.0 0.0 36-37 0.04750995683402501 0.0 0.0 38-39 0.0522553255979446 0.0 0.0 40-41 0.05829488584293317 0.0 0.0 42-43 0.06362170295341998 0.0 0.0 44-45 0.0690985712501177 0.0 0.0 46-47 0.07513813149510626 0.0 0.0 48-49 0.08241561402633474 0.0 0.0 50-51 0.09016200651447226 0.0 0.0 52-53 0.09815223218020247 0.0 0.0 54-55 0.10704276496319808 0.0 0.0 56-57 0.11632718210999726 0.0 0.0 58-59 0.1273371878982218 0.0 0.0 60-61 0.1390786932192244 0.0 0.0 62-63 0.15155169807300517 0.0 0.0 64-65 0.1643060489009313 0.0 0.0 66-67 0.1781482708288865 0.0 0.0 68-69 0.1950852984724414 0.0 3.7512796552724045E-5 70-71 0.2132227356056835 0.0 3.7512796552724045E-5 72-73 0.23413611968382714 0.0 3.7512796552724045E-5 74-75 0.25746907913962147 0.0 3.7512796552724045E-5 76-77 0.28406565189550287 0.0 3.7512796552724045E-5 78-79 0.31323185121524577 0.0 3.7512796552724045E-5 80-81 0.346712022138552 0.0 3.7512796552724045E-5 82-83 0.38461870305507967 0.0 3.7512796552724045E-5 84-85 0.4256014332889307 0.0 3.7512796552724045E-5 86-87 0.4733739796988248 0.0 3.7512796552724045E-5 88-89 0.5281426626658019 0.0 3.7512796552724045E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCGC 15 6.5717596E-4 95.17096 1 GTGTCGC 25 0.004986389 57.102573 1 CGAGGCG 135 0.0 52.872753 1 TCGGAAG 4435 0.0 51.195755 3 CGGAAGA 4465 0.0 50.532623 4 CGACGGG 110 0.0 47.585476 1 TTGGCCG 50 2.8277733E-5 47.500664 2 CGCGTTG 40 5.3960766E-4 47.488194 6 GAAGAGC 4795 0.0 46.943493 6 GATCGGA 4915 0.0 46.859352 1 ATCGGAA 5025 0.0 45.373775 2 AACCGAG 115 5.4569682E-11 41.378677 1 GCCGTCT 2755 0.0 40.689846 50-51 TATGCCG 2745 0.0 40.486675 48-49 CGTATGC 2780 0.0 40.148167 46-47 ATGCCGT 2790 0.0 40.003895 48-49 TGCCGTC 2780 0.0 39.9822 50-51 TCGTATG 2870 0.0 39.55111 44-45 CTCGTAT 2865 0.0 39.039925 44-45 TCTCGTA 2910 0.0 38.599422 42-43 >>END_MODULE