FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_35005-1.3410000003578e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_35005-1.3410000003578e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665757
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT215270.8075379713904907TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC52730.1978049762225139No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG52610.1973548226638812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGC156.5717596E-495.170961
GTGTCGC250.00498638957.1025731
CGAGGCG1350.052.8727531
TCGGAAG44350.051.1957553
CGGAAGA44650.050.5326234
CGACGGG1100.047.5854761
TTGGCCG502.8277733E-547.5006642
CGCGTTG405.3960766E-447.4881946
GAAGAGC47950.046.9434936
GATCGGA49150.046.8593521
ATCGGAA50250.045.3737752
AACCGAG1155.4569682E-1141.3786771
GCCGTCT27550.040.68984650-51
TATGCCG27450.040.48667548-49
CGTATGC27800.040.14816746-47
ATGCCGT27900.040.00389548-49
TGCCGTC27800.039.982250-51
TCGTATG28700.039.5511144-45
CTCGTAT28650.039.03992544-45
TCTCGTA29100.038.59942242-43
Produced by FastQC (version 0.11.2)