FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34344-1.34100000035857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34344-1.34100000035857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4870057
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1035202.125642471946427TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC81210.1667536950799549No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA75230.15447457801828604No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG60950.12515253928239445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA171950.066.157654
TCGGAAG172950.065.994843
GATCGGA175250.065.4252551
ATCGGAA177400.064.473262
GAAGAGC182700.062.2547346
GGAAGAG208000.054.8650555
TTGTGTT587300.046.271611
GCCGTCT130100.043.7782950-51
ATGCCGT130550.043.5481348-49
TGCCGTC130250.043.10787650-51
TCGTATG132600.043.0163844-45
CGTCTTC132650.042.9548452-53
TATGCCG131000.042.8728248-49
TCTCGTA130500.042.7443942-43
CGTATGC131600.042.63929446-47
AATCTCG130800.042.35717840-41
CTCGTAT130950.042.25273544-45
GTATGCC135850.042.00454746-47
ATCTCGT131400.041.83716642-43
CCGTCTT135200.041.5649452-53
Produced by FastQC (version 0.11.2)