##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34344-1.34100000035857.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4870057 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66864720474524 33.0 33.0 33.0 33.0 33.0 2 32.7164010606036 33.0 33.0 33.0 33.0 33.0 3 32.748094324152675 33.0 33.0 33.0 33.0 33.0 4 36.669866286985965 37.0 37.0 37.0 37.0 37.0 5 36.67403872275006 37.0 37.0 37.0 37.0 37.0 6 36.56141868565399 37.0 37.0 37.0 37.0 37.0 7 36.50752773530166 37.0 37.0 37.0 37.0 37.0 8 36.45325034183378 37.0 37.0 37.0 37.0 37.0 9 36.468319159303476 37.0 37.0 37.0 37.0 37.0 10-11 36.41958513011244 37.0 37.0 37.0 37.0 37.0 12-13 36.38816003590923 37.0 37.0 37.0 37.0 37.0 14-15 38.262149498455564 40.0 37.0 40.0 37.0 40.0 16-17 38.25397433746669 40.0 37.0 40.0 37.0 40.0 18-19 38.27262114180594 40.0 37.0 40.0 37.0 40.0 20-21 38.231459097911994 40.0 37.0 40.0 37.0 40.0 22-23 38.11187969668528 40.0 37.0 40.0 37.0 40.0 24-25 38.10514959065161 40.0 37.0 40.0 37.0 40.0 26-27 38.018316520730664 40.0 37.0 40.0 37.0 40.0 28-29 37.89962540479506 40.0 37.0 40.0 33.0 40.0 30-31 37.83240524700224 40.0 37.0 40.0 33.0 40.0 32-33 37.70391013903944 40.0 37.0 40.0 33.0 40.0 34-35 37.6197581465679 40.0 37.0 40.0 33.0 40.0 36-37 37.55180288444262 40.0 37.0 40.0 33.0 40.0 38-39 37.431797615510455 40.0 37.0 40.0 33.0 40.0 40-41 37.29843120932671 40.0 37.0 40.0 33.0 40.0 42-43 37.14789303287415 40.0 37.0 40.0 33.0 40.0 44-45 37.05810845745748 40.0 37.0 40.0 33.0 40.0 46-47 36.938206472737384 38.5 37.0 40.0 33.0 40.0 48-49 36.83492000196301 37.0 37.0 40.0 33.0 40.0 50-51 36.69329465753686 37.0 37.0 40.0 33.0 40.0 52-53 36.53666989934615 37.0 37.0 40.0 33.0 40.0 54-55 36.38512495849638 37.0 37.0 40.0 33.0 40.0 56-57 36.18735129794169 37.0 37.0 40.0 33.0 40.0 58-59 35.930973395177915 37.0 37.0 40.0 30.0 40.0 60-61 35.699027239311576 37.0 37.0 40.0 27.0 40.0 62-63 35.4490785220789 37.0 33.0 40.0 27.0 40.0 64-65 35.15522005183923 37.0 33.0 38.5 27.0 40.0 66-67 34.78464656573835 37.0 33.0 37.0 27.0 40.0 68-69 34.480675585521894 37.0 33.0 37.0 27.0 40.0 70-71 34.12184446711815 37.0 33.0 37.0 27.0 40.0 72-73 33.68167980374768 37.0 33.0 37.0 27.0 37.0 74-75 33.22286895204718 37.0 33.0 37.0 27.0 37.0 76-77 30.480733695724712 33.0 30.0 35.0 22.0 37.0 78-79 32.88708540782993 35.0 33.0 37.0 27.0 37.0 80-81 33.24063178315983 37.0 33.0 37.0 27.0 37.0 82-83 33.123373299326886 37.0 33.0 37.0 27.0 37.0 84-85 32.98932137755267 37.0 33.0 37.0 27.0 37.0 86-87 32.89899645938436 37.0 33.0 37.0 27.0 37.0 88-89 32.6445345506223 37.0 33.0 37.0 24.5 37.0 90-91 32.62068236573001 35.0 33.0 37.0 27.0 37.0 92-93 32.45744762330297 33.0 33.0 37.0 22.0 37.0 94-95 32.274564445549615 33.0 33.0 37.0 22.0 37.0 96-97 32.16336420703084 33.0 33.0 37.0 22.0 37.0 98-99 32.034598465685306 33.0 33.0 37.0 22.0 37.0 100-101 30.44362622449799 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.12663338599194418 2306 2 0.11592561675254842 2306 3 0.14288709568087654 2306 4 0.16460884078536964 2306 5 0.17326959915335038 2306 6 0.1864510774627277 2306 7 0.22734972566593115 2306 8 0.20081495172009767 2306 9 0.2057776581372721 2306 10-11 0.17770861708577712 2306 12-13 0.21606071409247107 2306 14-15 0.4175742016444772 2306 16-17 0.3988497951157939 2306 18-19 0.46315720001970817 2306 20-21 0.4263476966977038 2306 22-23 0.45841596669880147 2306 24-25 0.43357123910050177 2306 26-27 0.40087720794801385 2306 28-29 0.2945266409205587 2306 30-31 0.3897389686853856 2306 32-33 0.43852646364913284 2306 34-35 0.48012318850144453 2306 36-37 0.26379655593847673 2306 38-39 0.4516032549488713 2306 40-41 0.5002915513442119 2306 42-43 0.466077275264162 2306 44-45 0.4384811422186914 2306 46-47 0.5062821957260581 2306 48-49 0.44822829486333404 2306 50-51 0.4288197630162429 2306 52-53 0.4933393907145316 2306 54-55 0.31419266125615053 2306 56-57 0.3220559004859709 2306 58-59 0.5629162534377272 2306 60-61 0.5370302655765542 2306 62-63 0.42293680808964496 2306 64-65 0.4023470720374078 2306 66-67 0.4457773076602791 2306 68-69 0.430807459296247 2306 70-71 0.36310960038186124 2306 72-73 0.4223955938955797 2306 74-75 0.36668810213741665 2306 76-77 0.016281078696447793 2306 78-79 0.26527784237428875 2306 80-81 0.45884172367998133 2306 82-83 0.4910335924835678 2306 84-85 0.44935949879939585 2306 86-87 0.4020408005194369 2306 88-89 0.7846701819694033 2306 90-91 0.5326482037256497 2306 92-93 0.4117778795153768 2306 94-95 0.39926498127337595 2306 96-97 0.48799760540696013 2306 98-99 0.4147847666204072 2306 100-101 0.390408339183935 2307 1 0.1620794851831988 2307 2 0.12332343715175398 2307 3 0.10669261177412892 2307 4 0.16363073099024206 2307 5 0.17410929718501933 2307 6 0.1338131799374196 2307 7 0.18124572844954656 2307 8 0.20466539396490901 2307 9 0.19002438095041896 2307 10-11 0.17515516034810474 2307 12-13 0.1868373771002041 2307 14-15 0.34886294972929477 2307 16-17 0.3574940903403885 2307 18-19 0.24620680525782035 2307 20-21 0.27753770144599343 2307 22-23 0.26273503849413515 2307 24-25 0.2690086394968958 2307 26-27 0.31820333297612535 2307 28-29 0.2684930222411168 2307 30-31 0.32717406414145245 2307 32-33 0.39234557100860457 2307 34-35 0.3842893345220162 2307 36-37 0.35307065352261446 2307 38-39 0.47856288677358094 2307 40-41 0.37775114760479056 2307 42-43 0.3680711736122575 2307 44-45 0.3866210899682514 2307 46-47 0.45544985081932055 2307 48-49 0.30956407411195386 2307 50-51 0.39765971864758853 2307 52-53 0.29196464470972927 2307 54-55 0.2796367707783034 2307 56-57 0.294340146343707 2307 58-59 0.3064845759832693 2307 60-61 0.32897595049370665 2307 62-63 0.40510721027193597 2307 64-65 0.2974746415460672 2307 66-67 0.2021196903034408 2307 68-69 0.14115455562891555 2307 70-71 0.11464087663444644 2307 72-73 0.20572750145958452 2307 74-75 0.16457338185413306 2307 76-77 -0.21754637181654246 2307 78-79 0.13678265698903402 2307 80-81 0.271643370011013 2307 82-83 0.40986331141157706 2307 84-85 0.3289899970625214 2307 86-87 0.3533924298373563 2307 88-89 0.6295090091603939 2307 90-91 0.3569652762399542 2307 92-93 0.24821443982926183 2307 94-95 0.29643590249977336 2307 96-97 0.4134430702109668 2307 98-99 0.308176902525382 2307 100-101 0.2629549426169291 2201 1 0.10275221043009708 2201 2 0.10495682350327229 2201 3 0.06972420507902655 2201 4 0.156225384154844 2201 5 0.13478647924976883 2201 6 0.10786960205489748 2201 7 0.15228389032625245 2201 8 0.15120620039468946 2201 9 0.15226799240862476 2201 10-11 0.15382085563786774 2201 12-13 0.1882963119356873 2201 14-15 0.31482718152270905 2201 16-17 0.3187175317066888 2201 18-19 0.2877519563372246 2201 20-21 0.36649300016122766 2201 22-23 0.335467258520346 2201 24-25 0.35978424170945544 2201 26-27 0.3695276846250408 2201 28-29 0.40481276003665556 2201 30-31 0.3768855300531513 2201 32-33 0.463961249734858 2201 34-35 0.4487117578494164 2201 36-37 0.3775879188311464 2201 38-39 0.46164941260180825 2201 40-41 0.3766245111637261 2201 42-43 0.48060432867287517 2201 44-45 0.5505855303945779 2201 46-47 0.5353288699006384 2201 48-49 0.5273444030939061 2201 50-51 0.4116448541131632 2201 52-53 0.4245318876967872 2201 54-55 0.4687906446578367 2201 56-57 0.43881872108925535 2201 58-59 0.6327319615286697 2201 60-61 0.4841537857514666 2201 62-63 0.5882367152286463 2201 64-65 0.5857738308688454 2201 66-67 0.3711464700955105 2201 68-69 0.5350504163967287 2201 70-71 0.336886342607464 2201 72-73 0.40759489692066353 2201 74-75 0.30905934100844945 2201 76-77 -0.05426269909952097 2201 78-79 0.19298146621105872 2201 80-81 0.39027342945269794 2201 82-83 0.5060899159220824 2201 84-85 0.4493893234548878 2201 86-87 0.3468341401187729 2201 88-89 0.6076364838720636 2201 90-91 0.31842866415434656 2201 92-93 0.3777703396166672 2201 94-95 0.4770716271850901 2201 96-97 0.46467030286358835 2201 98-99 0.5698785731590661 2201 100-101 0.48869332740930815 1101 1 0.14298317845215536 1101 2 0.12397493117094172 1101 3 0.14402615526498863 1101 4 0.14537357919443394 1101 5 0.15256667509308386 1101 6 0.14552211470767418 1101 7 0.1877555622219731 1101 8 0.2200851637743284 1101 9 0.14830059330802214 1101 10-11 0.17461485861318238 1101 12-13 0.21902126675524158 1101 14-15 0.29406187710885945 1101 16-17 0.33340727984744944 1101 18-19 0.33845831582181773 1101 20-21 0.310395851240429 1101 22-23 0.30532985364004617 1101 24-25 0.32285502444127445 1101 26-27 0.3077494284545992 1101 28-29 0.35530836628272766 1101 30-31 0.3736359282092394 1101 32-33 0.440898769176151 1101 34-35 0.3985802026439629 1101 36-37 0.400386864123611 1101 38-39 0.4374711036493366 1101 40-41 0.4517420169863229 1101 42-43 0.3997966770116008 1101 44-45 0.4132715358097343 1101 46-47 0.5437730779811147 1101 48-49 0.4723598336731243 1101 50-51 0.38217901223232076 1101 52-53 0.37106755063688013 1101 54-55 0.3996366499263715 1101 56-57 0.4347392351542183 1101 58-59 0.4561941143323125 1101 60-61 0.4125502500886924 1101 62-63 0.40294655652768796 1101 64-65 0.43878267302476104 1101 66-67 0.4279606665282927 1101 68-69 0.37661423891516677 1101 70-71 0.23598165898556545 1101 72-73 0.3520373559300438 1101 74-75 0.2542963211500435 1101 76-77 -0.0664772067263435 1101 78-79 0.3036427860316522 1101 80-81 0.5324351067349227 1101 82-83 0.5711901243011255 1101 84-85 0.5809704367524802 1101 86-87 0.473256010138698 1101 88-89 0.7028450653500684 1101 90-91 0.619058341415915 1101 92-93 0.5890125783010589 1101 94-95 0.586239080667184 1101 96-97 0.6606048371546578 1101 98-99 0.6285072350996685 1101 100-101 0.507820522932569 2304 1 0.10185812447132747 2304 2 0.08909874699706677 2304 3 0.09402033497078577 2304 4 0.1087929056857746 2304 5 0.1131458971192103 2304 6 0.04809095861947554 2304 7 0.14618109761971 2304 8 0.08654030878996366 2304 9 0.11274943989068476 2304 10-11 0.07311952299055946 2304 12-13 0.16439778788041792 2304 14-15 0.2712738786763893 2304 16-17 0.26839590911874467 2304 18-19 0.2552357885170977 2304 20-21 0.26659904400423784 2304 22-23 0.2544390784116999 2304 24-25 0.16450248982580717 2304 26-27 0.1901817966003989 2304 28-29 0.24327349827612466 2304 30-31 0.23522972315696933 2304 32-33 0.14732337844667853 2304 34-35 0.212877725132806 2304 36-37 0.1854982600036621 2304 38-39 0.2008233812047635 2304 40-41 0.19847296359370858 2304 42-43 0.27502055567698136 2304 44-45 0.21152545465968586 2304 46-47 0.2668565994518204 2304 48-49 0.27825348420503104 2304 50-51 0.23031843448226397 2304 52-53 0.16117003545807052 2304 54-55 0.24806399363298937 2304 56-57 0.1977174068110088 2304 58-59 0.3192710709946951 2304 60-61 0.3018298370405148 2304 62-63 0.23553011365969212 2304 64-65 0.0608791835290603 2304 66-67 0.1866544332243052 2304 68-69 0.15920242881645663 2304 70-71 0.04064551015157036 2304 72-73 0.04332105826006938 2304 74-75 1.278065310756915E-4 2304 76-77 -0.05619238776735713 2304 78-79 0.1644791960855727 2304 80-81 0.23677063215388472 2304 82-83 0.37362238862738906 2304 84-85 0.3869206902677149 2304 86-87 0.33761563591333044 2304 88-89 0.5630034129070367 2304 90-91 0.2585768022015955 2304 92-93 0.494325315828263 2304 94-95 0.43795501503986145 2304 96-97 0.30658812661712176 2304 98-99 0.3469298279955879 2304 100-101 0.3626706697341078 2202 1 0.05144984085876558 2202 2 0.07205463115985822 2202 3 0.045598202846640845 2202 4 0.04657413120459353 2202 5 0.042717428526799495 2202 6 0.021828856675178088 2202 7 0.061910433615025795 2202 8 0.0458706176301078 2202 9 0.09176766436657147 2202 10-11 0.07245364450658798 2202 12-13 0.05853650688639789 2202 14-15 0.009878006740194678 2202 16-17 0.009735995788211937 2202 18-19 -0.011901682373441247 2202 20-21 0.06236393052257938 2202 22-23 0.08920139960400064 2202 24-25 0.11629395942286891 2202 26-27 0.08167929969963694 2202 28-29 0.03356713748099338 2202 30-31 0.0459187858492669 2202 32-33 0.07596844109840362 2202 34-35 0.11054938981178708 2202 36-37 0.11527143067048229 2202 38-39 0.08339774895994623 2202 40-41 0.20944234983519294 2202 42-43 0.19567210983047545 2202 44-45 0.2511640772960746 2202 46-47 0.30385046529271165 2202 48-49 0.22886660344665444 2202 50-51 0.25160080275114893 2202 52-53 0.13555878714549863 2202 54-55 0.0357133545408459 2202 56-57 0.1415039796460391 2202 58-59 0.19906249159351574 2202 60-61 0.1352576259736864 2202 62-63 0.1184719682470643 2202 64-65 0.18270173830133984 2202 66-67 0.1212444566841242 2202 68-69 0.05026602949455139 2202 70-71 -0.035878072071788836 2202 72-73 -0.04905029790223381 2202 74-75 -0.004868387492017234 2202 76-77 -0.33978283747362426 2202 78-79 -0.016015406738681293 2202 80-81 0.11338463144992517 2202 82-83 0.18749930388933223 2202 84-85 0.3453957309257305 2202 86-87 0.24900518209962996 2202 88-89 0.4411530095232479 2202 90-91 0.11431620963291067 2202 92-93 0.24908412675571157 2202 94-95 0.13445299950856793 2202 96-97 0.19159872153045399 2202 98-99 0.21502014247818124 2202 100-101 0.185230992601916 1102 1 0.10663469526994618 1102 2 0.07182170254210263 1102 3 0.07880006054091382 1102 4 0.08321881217587901 1102 5 0.06944349958099139 1102 6 0.015816059937868943 1102 7 0.019871625109843194 1102 8 0.03994856354040621 1102 9 0.06434449412543586 1102 10-11 0.0693460461591826 1102 12-13 0.046704542704446794 1102 14-15 0.08478758034251399 1102 16-17 0.1892400484256953 1102 18-19 0.12854272046656234 1102 20-21 0.13835819923045278 1102 22-23 0.0892170856007013 1102 24-25 0.0384957167605009 1102 26-27 0.06712632108909844 1102 28-29 0.07159092116266663 1102 30-31 0.12125382102890825 1102 32-33 0.15646551852734802 1102 34-35 0.08753794831086026 1102 36-37 0.07272830893786875 1102 38-39 0.03878365399557282 1102 40-41 0.021380658328148172 1102 42-43 0.04421976363547486 1102 44-45 -0.014469421920892955 1102 46-47 0.09715068936633031 1102 48-49 0.018502376289809774 1102 50-51 0.05805204839639089 1102 52-53 0.07983984984647918 1102 54-55 0.034947017701774996 1102 56-57 0.018766958754149243 1102 58-59 0.061716494769505914 1102 60-61 0.003569719961483031 1102 62-63 0.04549531423882058 1102 64-65 -0.011692457542544332 1102 66-67 -0.023161840990546523 1102 68-69 -0.05128604084617905 1102 70-71 -0.23832441289138018 1102 72-73 -0.09558238546382825 1102 74-75 -0.07158067848244087 1102 76-77 -0.15370584621645733 1102 78-79 -0.03822500707466503 1102 80-81 -0.07722923918782953 1102 82-83 0.12237770789819535 1102 84-85 0.08563410747566991 1102 86-87 0.017975244615918484 1102 88-89 0.2799804897028082 1102 90-91 0.03583098778147331 1102 92-93 0.03972388772136526 1102 94-95 0.13776890114555584 1102 96-97 -0.0017364165358273453 1102 98-99 -0.06308507345305259 1102 100-101 -0.012823100473390525 2305 1 0.08209932067853032 2305 2 0.055633869634277744 2305 3 0.0318955040836002 2305 4 0.03895814553490595 2305 5 0.06265437917424066 2305 6 0.03755330380455746 2305 7 0.012749263291603086 2305 8 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-1.3534548347164304 1213 84-85 -1.344327540868484 1213 86-87 -1.0602962083235816 1213 88-89 -0.7826830969742034 1213 90-91 -0.8333558627175783 1213 92-93 -1.0076109225795342 1213 94-95 -0.8693720442115307 1213 96-97 -0.9213690625842368 1213 98-99 -1.038933200205193 1213 100-101 -0.8315945178701227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 8.0 9 48.0 10 64.0 11 46.0 12 80.0 13 176.0 14 509.0 15 1147.0 16 2215.0 17 3618.0 18 5487.0 19 7631.0 20 10043.0 21 13034.0 22 16452.0 23 20604.0 24 26000.0 25 32146.0 26 41344.0 27 53644.0 28 73777.0 29 104332.0 30 112473.0 31 112548.0 32 143686.0 33 200104.0 34 303975.0 35 525494.0 36 1039160.0 37 1608060.0 38 411923.0 39 228.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.084617321165894 24.953763274547423 23.936760993974417 15.02485841031227 2 28.853029851601324 23.762884089447002 33.706299536124526 13.677786522827146 3 37.03707506243789 19.112885954301394 27.445488629557175 16.404550353703534 4 28.03495126887811 19.145914051260355 34.492597654785875 18.326537025075655 5 33.568190269641605 18.05237597835097 32.115578934702405 16.263854817305013 6 26.883709985324604 19.385214587837474 37.48103974963743 16.250035677200493 7 31.991206673761724 21.508126085587907 29.15594211730992 17.344725123340446 8 27.88665512539176 28.603505051378246 26.41045063743607 17.099389185793925 9 31.764659017337994 23.853724915334666 25.759164625793908 18.622451441533435 10-11 24.973976041017394 28.802842445680277 28.928129109637172 17.29505240366515 12-13 23.99761440163842 27.077475684576175 28.848173645606202 20.0767362681792 14-15 22.97403295279706 27.54435933706731 29.596583777150865 19.88502393298477 16-17 23.127881098066496 27.125840850193626 30.478923342142032 19.26735470959784 18-19 21.203909933703034 27.37741057240192 31.672473237992904 19.746206255902138 20-21 22.480373433000885 27.50985255408715 31.188413195163832 18.82136081774813 22-23 21.35116590967402 29.85496931903105 29.875195031825726 18.918669739469205 24-25 21.39656681636375 27.134702940848538 31.154922827391957 20.313807415395754 26-27 21.311711546702636 27.362708485752833 29.63593649930586 21.689643468238668 28-29 21.986292973573 28.826099981991998 29.427561114787775 19.76004592964723 30-31 20.46970908143375 28.17419796113269 30.50453002911465 20.85156292831891 32-33 20.380582814533792 29.20526392196231 29.832987581048847 20.58116568245505 34-35 20.6381157345797 29.361257989382878 31.072336525013977 18.928289751023446 36-37 20.46530399615361 28.477087519446787 31.09402722226734 19.963581262132262 38-39 22.21571415415141 29.345474410955102 29.297107087881606 19.141704347011878 40-41 20.87910542174458 30.375883078356708 29.088834907944854 19.65617659195386 42-43 20.922811809952528 29.36143885220044 30.547939340556535 19.167809997290504 44-45 20.529213595389834 28.26930739585486 30.822346978764042 20.37913202999126 46-47 21.894732302915685 28.105616782378174 29.506752746993246 20.492898167712895 48-49 20.84665804603189 29.60214682144382 30.1326300472451 19.418565085279187 50-51 22.000134090689045 28.411660879513768 29.81355541872692 19.774649611070263 52-53 20.579140879051177 28.174588466771848 28.954508012799618 22.291762641377357 54-55 22.061153283039165 28.36437367268293 29.83535133273595 19.73912171154196 56-57 20.86596902380247 28.403268080436085 29.58975658625167 21.141006309509773 58-59 20.81668829866514 28.33142788955594 29.947599525934216 20.904284285844703 60-61 21.72645130049252 28.64966889450409 29.93579141929888 19.688088385704503 62-63 20.566023207249238 28.620275439781683 29.852506042057236 20.961195310911844 64-65 21.94838022435443 28.41669090822091 29.723270464791973 19.91165840263269 66-67 21.045898586539703 30.418502397190707 28.45273618249255 20.082862833777043 68-69 21.245025651020637 30.406371438526325 28.358838878894467 19.989764031558575 70-71 20.976955094895043 31.03216020296204 28.25024425880125 19.740640443341665 72-73 20.64163011451922 31.491789759510663 28.07685553283634 19.789724593133776 74-75 21.098676450078862 30.78909570190827 28.024417040538353 20.08781080747452 76-77 21.190169977624308 30.141597693945787 28.389687502375093 20.278544826054816 78-79 20.9686551703176 29.943229520074528 28.864019472379354 20.22409583722852 80-81 20.59346353472505 30.094299557940662 28.803299817105803 20.50893709022849 82-83 19.912983948118498 30.1307838302956 28.995840059005907 20.960392162579996 84-85 19.996792321944092 29.893937159902954 29.061943097187715 21.047327420965235 86-87 20.01536641885013 30.003908352722775 29.463382965784557 20.517342262642536 88-89 20.130596156626666 30.23823303736905 29.259870907214868 20.371299898789417 90-91 20.3327896071205 29.87758766301517 29.01472866815969 20.77489406170464 92-93 19.97070448412848 30.578460616265822 28.781678562599005 20.66915633700669 94-95 19.679791436498984 30.9313634573923 28.766303266168013 20.622541839940702 96-97 20.056179452188722 31.08375897881234 28.50160330799863 20.358458261000305 98-99 20.369342556815017 30.44714385866604 28.226450347441684 20.95706323707726 100-101 20.399581743985465 30.46318167222928 27.77596995358541 21.361266630199847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 56.0 1 46.0 2 75.5 3 203.0 4 249.5 5 174.5 6 119.0 7 104.0 8 114.5 9 117.0 10 117.5 11 124.5 12 123.0 13 127.5 14 145.0 15 157.5 16 184.0 17 253.5 18 326.5 19 403.0 20 576.5 21 877.0 22 1375.5 23 2161.5 24 3271.0 25 4990.5 26 7863.0 27 11716.5 28 15900.5 29 20371.5 30 25974.0 31 32893.5 32 45788.0 33 69124.0 34 102988.5 35 144444.5 36 201199.0 37 270729.5 38 331922.0 39 355155.0 40 348914.5 41 325482.0 42 299126.0 43 297509.5 44 289374.0 45 266441.0 46 249463.0 47 219978.0 48 176130.0 49 144633.0 50 122129.5 51 103026.5 52 87059.0 53 71031.5 54 54819.0 55 40500.0 56 30346.5 57 22670.5 58 17534.5 59 13748.5 60 10346.0 61 7663.0 62 5642.5 63 4143.0 64 2990.0 65 2025.0 66 1438.5 67 1019.5 68 695.0 69 514.5 70 347.0 71 233.5 72 168.5 73 118.5 74 78.5 75 54.5 76 39.5 77 28.0 78 22.5 79 18.0 80 12.5 81 6.5 82 6.5 83 6.5 84 3.0 85 2.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1667536950799549 2 0.0 3 3.696055302843478E-4 4 0.020369371446781834 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.236078345694928E-4 12-13 0.0 14-15 0.0 16-17 0.0012422852545668356 18-19 0.0 20-21 0.0 22-23 3.7987235057002414E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.494778808543719E-4 38-39 6.87876959140314E-4 40-41 0.0047535377922681395 42-43 0.003028711984274517 44-45 7.905451619970771E-4 46-47 0.0038397907868429467 48-49 0.00710463963768802 50-51 0.004486600464840555 52-53 0.0013860207385663042 54-55 0.01040028894939012 56-57 0.018459742873646035 58-59 0.02284367513562983 60-61 0.0110162981665307 62-63 5.544082954265217E-4 64-65 0.013624070519092486 66-67 0.03388050694273188 68-69 0.019558292644213406 70-71 0.03608787330415229 72-73 0.029609509703890532 74-75 0.028911365924464538 76-77 0.037535494964432654 78-79 0.016447446097653477 80-81 0.011806843328527777 82-83 7.392110605686956E-4 84-85 0.010082017520534154 86-87 0.02049257329020995 88-89 0.020554174211924008 90-91 0.024332364077052897 92-93 0.042638104646413794 94-95 0.02718654011647092 96-97 0.028192688504467198 98-99 0.03609814012443797 100-101 0.016231842871654273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4870057.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.40486701604933 #Duplication Level Percentage of deduplicated Percentage of total 1 91.15918356508064 43.21388974194277 2 3.9706852421929812 3.7645961173749583 3 1.4500934387608921 2.0622445987591727 4 0.7466359416704803 1.4157671009716752 5 0.3879360063570246 0.9195027396046006 6 0.3616570819464892 1.0286583525051465 7 0.20903541502403883 0.6936507235601469 8 0.18308008717784643 0.6943109748762022 9 0.12216953092953856 0.5212287330415111 >10 1.1003595535124724 10.123386928885857 >50 0.13386181566721278 4.450881241473711 >100 0.13887557299640052 13.69741430974327 >500 0.0279649569206665 9.233006966183085 >1k 0.008288500040832415 5.609000093621137 >5k 1.299688962832529E-4 0.4464578606666026 >10k+ 4.33228264229276E-5 2.126003516790178 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 103520 2.125642471946427 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8121 0.1667536950799549 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7523 0.15447457801828604 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6095 0.12515253928239445 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014784221211373912 0.0 0.0 2 0.0015400230428514491 0.0 2.0533640571352656E-5 3 0.0016426912457082124 0.0 2.0533640571352656E-5 4 0.0017658930891363285 0.0 2.0533640571352656E-5 5 0.0019096285731357969 0.0 2.0533640571352656E-5 6 0.002073897697706618 0.0 2.0533640571352656E-5 7 0.002484570509133671 0.0 2.0533640571352656E-5 8 0.0029157769611320773 0.0 2.0533640571352656E-5 9 0.003388050694273188 0.0 2.0533640571352656E-5 10-11 0.005574883415122247 0.0 2.0533640571352656E-5 12-13 0.007433177886829662 0.0 2.0533640571352656E-5 14-15 0.009548142865678986 0.0 2.0533640571352656E-5 16-17 0.012320184342811593 0.0 2.0533640571352656E-5 18-19 0.015277028585086377 0.0 2.0533640571352656E-5 20-21 0.01892174978650147 0.0 2.0533640571352656E-5 22-23 0.023357016149913645 0.0 2.0533640571352656E-5 24-25 0.02826455624646693 0.0 2.0533640571352656E-5 26-27 0.03366490371673268 0.0 2.0533640571352656E-5 28-29 0.03953752492013954 0.0 2.0533640571352656E-5 30-31 0.05930115397006647 0.0 2.0533640571352656E-5 32-33 0.06694993508289535 0.0 4.106728114270531E-5 34-35 0.0741675097437258 0.0 4.106728114270531E-5 36-37 0.08186762495798304 0.0 4.106728114270531E-5 38-39 0.09060468902109359 0.0 5.133410142838164E-5 40-41 0.10006043050420149 0.0 8.213456228541063E-5 42-43 0.10968044111188022 0.0 8.213456228541063E-5 44-45 0.12076860702041065 0.0 8.213456228541063E-5 46-47 0.13194917431151215 0.0 8.213456228541063E-5 48-49 0.1433453448286129 1.0266820285676328E-5 1.0266820285676327E-4 50-51 0.1560043342408518 2.0533640571352656E-5 1.0266820285676327E-4 52-53 0.17044975038279842 2.0533640571352656E-5 1.0266820285676327E-4 54-55 0.1859321153735983 2.0533640571352656E-5 1.129350231424396E-4 56-57 0.20190728773811067 2.0533640571352656E-5 1.2320184342811592E-4 58-59 0.2192376803803323 2.0533640571352656E-5 1.437354839994686E-4 60-61 0.2383236992914046 2.0533640571352656E-5 1.437354839994686E-4 62-63 0.2592269453930416 2.0533640571352656E-5 1.437354839994686E-4 64-65 0.2813724767492454 2.0533640571352656E-5 1.437354839994686E-4 66-67 0.30657752055058085 2.0533640571352656E-5 1.437354839994686E-4 68-69 0.33383592840905146 2.0533640571352656E-5 1.437354839994686E-4 70-71 0.36326063534779984 2.0533640571352656E-5 1.437354839994686E-4 72-73 0.3948927086479686 2.0533640571352656E-5 1.437354839994686E-4 74-75 0.42911202066012777 2.0533640571352656E-5 1.540023042851449E-4 76-77 0.46769473129369943 2.0533640571352656E-5 1.6426912457082125E-4 78-79 0.5104149705023986 2.0533640571352656E-5 1.6426912457082125E-4 80-81 0.5573856733093678 2.0533640571352656E-5 1.848027651421739E-4 82-83 0.6084220369494648 2.0533640571352656E-5 1.848027651421739E-4 84-85 0.6644172747875435 2.0533640571352656E-5 1.848027651421739E-4 86-87 0.7240161665458946 2.0533640571352656E-5 1.848027651421739E-4 88-89 0.7910482361910753 2.0533640571352656E-5 1.848027651421739E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 17195 0.0 66.15765 4 TCGGAAG 17295 0.0 65.99484 3 GATCGGA 17525 0.0 65.425255 1 ATCGGAA 17740 0.0 64.47326 2 GAAGAGC 18270 0.0 62.254734 6 GGAAGAG 20800 0.0 54.865055 5 TTGTGTT 58730 0.0 46.27161 1 GCCGTCT 13010 0.0 43.77829 50-51 ATGCCGT 13055 0.0 43.54813 48-49 TGCCGTC 13025 0.0 43.107876 50-51 TCGTATG 13260 0.0 43.01638 44-45 CGTCTTC 13265 0.0 42.95484 52-53 TATGCCG 13100 0.0 42.87282 48-49 TCTCGTA 13050 0.0 42.74439 42-43 CGTATGC 13160 0.0 42.639294 46-47 AATCTCG 13080 0.0 42.357178 40-41 CTCGTAT 13095 0.0 42.252735 44-45 GTATGCC 13585 0.0 42.004547 46-47 ATCTCGT 13140 0.0 41.837166 42-43 CCGTCTT 13520 0.0 41.56494 52-53 >>END_MODULE