##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34311-1.34100000035764.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2663197 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.671237989529125 33.0 33.0 33.0 33.0 33.0 2 32.71740055279425 33.0 33.0 33.0 33.0 33.0 3 32.74243512590319 33.0 33.0 33.0 33.0 33.0 4 36.67801555799289 37.0 37.0 37.0 37.0 37.0 5 36.67934065711248 37.0 37.0 37.0 37.0 37.0 6 36.578576425251306 37.0 37.0 37.0 37.0 37.0 7 36.539686324368795 37.0 37.0 37.0 37.0 37.0 8 36.47487099151884 37.0 37.0 37.0 37.0 37.0 9 36.48272283274576 37.0 37.0 37.0 37.0 37.0 10-11 36.44276953601255 37.0 37.0 37.0 37.0 37.0 12-13 36.41785418052063 37.0 37.0 37.0 37.0 37.0 14-15 38.309852969945524 40.0 37.0 40.0 37.0 40.0 16-17 38.305251169928475 40.0 37.0 40.0 37.0 40.0 18-19 38.329434322733164 40.0 37.0 40.0 37.0 40.0 20-21 38.27964754391057 40.0 37.0 40.0 37.0 40.0 22-23 38.193319345132934 40.0 37.0 40.0 37.0 40.0 24-25 38.17001859043849 40.0 37.0 40.0 37.0 40.0 26-27 38.07461933908757 40.0 37.0 40.0 37.0 40.0 28-29 37.98345616189865 40.0 37.0 40.0 37.0 40.0 30-31 37.931763966390776 40.0 37.0 40.0 33.0 40.0 32-33 37.8271147421689 40.0 37.0 40.0 33.0 40.0 34-35 37.76162165247257 40.0 37.0 40.0 33.0 40.0 36-37 37.68952221709472 40.0 37.0 40.0 33.0 40.0 38-39 37.590196669641784 40.0 37.0 40.0 33.0 40.0 40-41 37.51148619497543 40.0 37.0 40.0 33.0 40.0 42-43 37.378641722711464 40.0 37.0 40.0 33.0 40.0 44-45 37.29737811359806 40.0 37.0 40.0 33.0 40.0 46-47 37.158379571620124 40.0 37.0 40.0 33.0 40.0 48-49 37.07725001192176 40.0 37.0 40.0 33.0 40.0 50-51 36.910134511265966 37.0 37.0 40.0 33.0 40.0 52-53 36.7774580701315 37.0 37.0 40.0 33.0 40.0 54-55 36.65843796009082 37.0 37.0 40.0 33.0 40.0 56-57 36.453010610931145 37.0 37.0 40.0 33.0 40.0 58-59 36.16063419266393 37.0 37.0 40.0 33.0 40.0 60-61 35.93411640220381 37.0 37.0 40.0 33.0 40.0 62-63 35.68866816837057 37.0 37.0 40.0 33.0 40.0 64-65 35.40732886076396 37.0 33.0 37.0 27.0 40.0 66-67 35.126550157573774 37.0 33.0 37.0 27.0 40.0 68-69 34.83939791160774 37.0 33.0 37.0 27.0 40.0 70-71 34.493724647481955 37.0 33.0 37.0 27.0 40.0 72-73 34.200788751263985 37.0 33.0 37.0 27.0 37.0 74-75 33.83683689190097 37.0 33.0 37.0 27.0 37.0 76-77 31.071830585570652 33.0 30.0 35.0 24.5 37.0 78-79 33.51946889396466 35.0 33.0 37.0 27.0 37.0 80-81 33.92888866276134 37.0 33.0 37.0 27.0 37.0 82-83 33.85118975426903 37.0 33.0 37.0 27.0 37.0 84-85 33.7392153115222 37.0 33.0 37.0 27.0 37.0 86-87 33.67474899528649 37.0 33.0 37.0 27.0 37.0 88-89 33.43341743025394 37.0 33.0 37.0 27.0 37.0 90-91 33.44408487242964 37.0 33.0 37.0 27.0 37.0 92-93 33.30521531077123 37.0 33.0 37.0 27.0 37.0 94-95 33.14430963988019 37.0 33.0 37.0 27.0 37.0 96-97 33.04904425020004 37.0 33.0 37.0 27.0 37.0 98-99 32.93549106581301 33.0 33.0 37.0 27.0 37.0 100-101 31.365361067919494 33.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.11229928085512597 2306 2 0.16626754185590897 2306 3 0.14222276658252042 2306 4 0.1955699674742135 2306 5 0.18478262653314204 2306 6 0.14664026584870982 2306 7 0.20308574444296568 2306 8 0.23077634594829277 2306 9 0.1380281132324086 2306 10-11 0.22966455641180517 2306 12-13 0.25170264622585137 2306 14-15 0.4652958783816459 2306 16-17 0.4722285027507951 2306 18-19 0.46900800883800997 2306 20-21 0.4653433082350702 2306 22-23 0.4559458795562108 2306 24-25 0.5574627682535365 2306 26-27 0.48637310385949917 2306 28-29 0.5085816088542856 2306 30-31 0.5528223498244458 2306 32-33 0.5059983973290301 2306 34-35 0.5496487312714962 2306 36-37 0.5710321575117376 2306 38-39 0.5448313536557734 2306 40-41 0.7083071864140749 2306 42-43 0.5555301127281922 2306 44-45 0.6609919200803844 2306 46-47 0.7552360853012772 2306 48-49 0.6247268939927366 2306 50-51 0.5917234204659607 2306 52-53 0.7009888543796592 2306 54-55 0.6468546370807005 2306 56-57 0.6677667947067576 2306 58-59 0.7686030809975151 2306 60-61 0.7388748969385546 2306 62-63 0.6947374671078919 2306 64-65 0.5147268858053309 2306 66-67 0.4895057365849951 2306 68-69 0.6198462638906861 2306 70-71 0.6198430494173266 2306 72-73 0.5317798001233882 2306 74-75 0.5464763858857111 2306 76-77 0.09167978521305642 2306 78-79 0.5455159764784554 2306 80-81 0.6604112059160272 2306 82-83 0.6196108188219256 2306 84-85 0.6180405147925399 2306 86-87 0.5975273222087623 2306 88-89 0.7836818430027535 2306 90-91 0.7186397591076457 2306 92-93 0.5354224025599947 2306 94-95 0.6182188810715488 2306 96-97 0.498861321729386 2306 98-99 0.5157750385633406 2306 100-101 0.3713292811546296 2307 1 0.13196799985931307 2307 2 0.08973553544058888 2307 3 0.11728408338419882 2307 4 0.15903154906148131 2307 5 0.15185303308866338 2307 6 0.16026991319715478 2307 7 0.16872674069460203 2307 8 0.19872798686314752 2307 9 0.18618287825180602 2307 10-11 0.1526686768676555 2307 12-13 0.22933872882765627 2307 14-15 0.4325008372144552 2307 16-17 0.2990277449390817 2307 18-19 0.3285246339346628 2307 20-21 0.3317154549169814 2307 22-23 0.2869760433528441 2307 24-25 0.4285554573306314 2307 26-27 0.3919651629495391 2307 28-29 0.3884513423161877 2307 30-31 0.4462208164268304 2307 32-33 0.3632704080466951 2307 34-35 0.38265689660136815 2307 36-37 0.3791672546426952 2307 38-39 0.5090180225151428 2307 40-41 0.43808260869642623 2307 42-43 0.41781959447766326 2307 44-45 0.32643349656795095 2307 46-47 0.4844226337280588 2307 48-49 0.415957407791403 2307 50-51 0.4240820803585095 2307 52-53 0.28930622831787645 2307 54-55 0.4328670972860351 2307 56-57 0.4632278148289757 2307 58-59 0.49630955437402946 2307 60-61 0.5786584338860266 2307 62-63 0.5027143407275858 2307 64-65 0.6110850041664833 2307 66-67 0.5613201075987817 2307 68-69 0.3603580442747898 2307 70-71 0.3925875753406558 2307 72-73 0.37519583327307515 2307 74-75 0.3894057052422113 2307 76-77 0.09640904858917665 2307 78-79 0.3138730184213898 2307 80-81 0.4200709381570036 2307 82-83 0.429993022040712 2307 84-85 0.4697614135020842 2307 86-87 0.5085072863779914 2307 88-89 0.7168737303310024 2307 90-91 0.48633898991712954 2307 92-93 0.5116934440029297 2307 94-95 0.4997767472717598 2307 96-97 0.5138987845717864 2307 98-99 0.41303357744188673 2307 100-101 0.5064849672757319 2201 1 0.09665696427237691 2201 2 0.052121151847451586 2201 3 0.06664386968701308 2201 4 0.09515063758779974 2201 5 0.10851163176684508 2201 6 0.14170601514643266 2201 7 0.16411837256023176 2201 8 0.12870955171848664 2201 9 0.18857568478311748 2201 10-11 0.08304068220171956 2201 12-13 0.12080296422898584 2201 14-15 0.2859497977816403 2201 16-17 0.39343700515051694 2201 18-19 0.24355283009128925 2201 20-21 0.3260565761583649 2201 22-23 0.3656452014812359 2201 24-25 0.3962881017497111 2201 26-27 0.35576790272137515 2201 28-29 0.4037219961576781 2201 30-31 0.3859684096162752 2201 32-33 0.45467820933172476 2201 34-35 0.3956935954981802 2201 36-37 0.3009855200669804 2201 38-39 0.4268612359991337 2201 40-41 0.34930923556660076 2201 42-43 0.45854955932130537 2201 44-45 0.4845146530075084 2201 46-47 0.4703500552741815 2201 48-49 0.29672633307681195 2201 50-51 0.37262378099014626 2201 52-53 0.41341404674214743 2201 54-55 0.3681242069396191 2201 56-57 0.4497116349588097 2201 58-59 0.4814548408712227 2201 60-61 0.42917783677127375 2201 62-63 0.5118340939223813 2201 64-65 0.42657261583288175 2201 66-67 0.3015287884199509 2201 68-69 0.23329015194271818 2201 70-71 0.3425773335936242 2201 72-73 0.3111987093228592 2201 74-75 0.2814087819466238 2201 76-77 0.1666285564000738 2201 78-79 0.290175287908923 2201 80-81 0.39338763387679165 2201 82-83 0.4982168522868946 2201 84-85 0.5828955135033382 2201 86-87 0.4815710736506347 2201 88-89 0.5184952706061452 2201 90-91 0.33468421621793 2201 92-93 0.6643000731304554 2201 94-95 0.5013113372047542 2201 96-97 0.5697823547193863 2201 98-99 0.5836811484757121 2201 100-101 0.39408618290839925 1101 1 0.12984982901339492 1101 2 0.08701464119638302 1101 3 0.1248510540418053 1101 4 0.1179870121343427 1101 5 0.12786629450584996 1101 6 0.05730212021148162 1101 7 0.03815486711877725 1101 8 0.07398953426219634 1101 9 0.09518316371282509 1101 10-11 0.09068394652815215 1101 12-13 0.08493301359756344 1101 14-15 0.14592852292688008 1101 16-17 0.23127419796070825 1101 18-19 0.1587902629254856 1101 20-21 0.16736252493506498 1101 22-23 0.21837888394039595 1101 24-25 0.13430599444807712 1101 26-27 0.08842957797981654 1101 28-29 0.1410402734399483 1101 30-31 0.1824676049903502 1101 32-33 0.27898541277934186 1101 34-35 0.14320130081416238 1101 36-37 0.10659267169123154 1101 38-39 0.17609547760510935 1101 40-41 0.24024560193050348 1101 42-43 0.14529096073417236 1101 44-45 0.09831057805455856 1101 46-47 0.19540324241107498 1101 48-49 0.2226830196545606 1101 50-51 0.1875570925031198 1101 52-53 0.16070491219232963 1101 54-55 0.2686110738081169 1101 56-57 0.2846658121947172 1101 58-59 0.2672661492747892 1101 60-61 0.25341003271813634 1101 62-63 0.3509518693362921 1101 64-65 0.2085387669934491 1101 66-67 0.21384092872489902 1101 68-69 0.18645819601985636 1101 70-71 0.13425286478310738 1101 72-73 0.14805262198685654 1101 74-75 -0.022527066183641864 1101 76-77 -0.26635155979336744 1101 78-79 0.08411195430284124 1101 80-81 0.36224792899721336 1101 82-83 0.44150436285612926 1101 84-85 0.4262962172100728 1101 86-87 0.5059594215763354 1101 88-89 0.6095700599032625 1101 90-91 0.4745063802525209 1101 92-93 0.4713050918141448 1101 94-95 0.42028687112178176 1101 96-97 0.5213125077346845 1101 98-99 0.4739363103555476 1101 100-101 0.5217571318214738 2304 1 0.13962338279765873 2304 2 0.15347585975953848 2304 3 0.09575806720657454 2304 4 0.18147552093205377 2304 5 0.1104840689106723 2304 6 0.11735862209560821 2304 7 0.12584772147288703 2304 8 0.1237769941778808 2304 9 0.08375798264797396 2304 10-11 0.1737497220910953 2304 12-13 0.14642487390455017 2304 14-15 0.29809932706977094 2304 16-17 0.272583852851767 2304 18-19 0.31325163063957007 2304 20-21 0.28107695353343587 2304 22-23 0.36852024832051455 2304 24-25 0.2642552831297138 2304 26-27 0.20612754294689495 2304 28-29 0.25897611221656547 2304 30-31 0.29045924376433163 2304 32-33 0.22047339077519013 2304 34-35 0.3020592213869051 2304 36-37 0.24072268305302202 2304 38-39 0.31225043226761073 2304 40-41 0.28274045841659046 2304 42-43 0.4650144173509929 2304 44-45 0.4439147928845486 2304 46-47 0.4403749914690138 2304 48-49 0.36291319464370275 2304 50-51 0.3500794103086733 2304 52-53 0.36901272901743454 2304 54-55 0.4348536107778145 2304 56-57 0.32332540589002434 2304 58-59 0.5106221330787477 2304 60-61 0.4938989187799834 2304 62-63 0.46781151830517587 2304 64-65 0.35854611487046384 2304 66-67 0.37643562860250057 2304 68-69 0.3704635128120586 2304 70-71 0.2976981636771896 2304 72-73 0.2694297896105766 2304 74-75 0.25180098691409114 2304 76-77 0.1604743328091871 2304 78-79 0.2062248966559963 2304 80-81 0.3642786847976467 2304 82-83 0.48380817453991654 2304 84-85 0.6146036611578367 2304 86-87 0.49573490554919175 2304 88-89 0.6393479346778861 2304 90-91 0.4165094773278426 2304 92-93 0.5021711104053708 2304 94-95 0.4504627376229209 2304 96-97 0.39351055385742484 2304 98-99 0.5022865035937372 2304 100-101 0.521800999743931 2202 1 0.09828513408236006 2202 2 0.11552441525071089 2202 3 0.08806083655851893 2202 4 0.051514980315779724 2202 5 0.12205263621694229 2202 6 0.0955097871320234 2202 7 0.11912296392846144 2202 8 0.0739345287616473 2202 9 0.07091304439320112 2202 10-11 0.12661100077204424 2202 12-13 0.07848467645615642 2202 14-15 0.08562133836227304 2202 16-17 0.024271574621451464 2202 18-19 -0.023631248456418064 2202 20-21 0.0897264921010077 2202 22-23 0.14960719907959685 2202 24-25 0.08940458122983586 2202 26-27 0.016478152067989527 2202 28-29 0.00919631981381741 2202 30-31 0.11405345588313764 2202 32-33 0.08441614816057097 2202 34-35 0.1761749827977539 2202 36-37 0.09058607103116856 2202 38-39 0.2264868628974881 2202 40-41 0.23281562816389823 2202 42-43 0.239437106117947 2202 44-45 0.13843497510964653 2202 46-47 0.3519404345918318 2202 48-49 0.17519634791046457 2202 50-51 0.18439004502914003 2202 52-53 0.1680460309195837 2202 54-55 0.14008860617673946 2202 56-57 0.16356697154142097 2202 58-59 0.24656927189474231 2202 60-61 0.14572618741053134 2202 62-63 0.39669317423601314 2202 64-65 0.21814568922413002 2202 66-67 0.08683111235864516 2202 68-69 0.05697101653267822 2202 70-71 -0.061666150195321734 2202 72-73 0.058558672591914274 2202 74-75 0.15867797739764455 2202 76-77 -0.2285098910111003 2202 78-79 0.21771819954274463 2202 80-81 0.3030683571938724 2202 82-83 0.48792688972420706 2202 84-85 0.3862860623484323 2202 86-87 0.43462786534379205 2202 88-89 0.6021252769634202 2202 90-91 0.2812318604928521 2202 92-93 0.28626447236758423 2202 94-95 0.31779258323145854 2202 96-97 0.5107302956673294 2202 98-99 0.40715540149542306 2202 100-101 0.2507154122649027 1102 1 0.15111799333980258 1102 2 0.1333564900660278 1102 3 0.06741227345185763 1102 4 0.10393242630864563 1102 5 0.1029865998324695 1102 6 0.06413483061739811 1102 7 0.1145916169390162 1102 8 0.103867134951237 1102 9 0.06493457221361609 1102 10-11 0.12290621703932203 1102 12-13 0.0604551268623581 1102 14-15 0.10113385191947799 1102 16-17 0.17415919476047037 1102 18-19 0.12433789909373161 1102 20-21 0.14030271600125133 1102 22-23 0.1599130632960737 1102 24-25 0.06395523231679334 1102 26-27 0.043382641123322685 1102 28-29 0.08860334977951112 1102 30-31 0.11892347723360785 1102 32-33 0.13323402798403805 1102 34-35 0.15592699676764 1102 36-37 0.08690823118014634 1102 38-39 0.2304227159953527 1102 40-41 0.2061353872378433 1102 42-43 0.16052410416024543 1102 44-45 0.11511658028286575 1102 46-47 0.21379454882024618 1102 48-49 0.12776863595761512 1102 50-51 0.1299519784402392 1102 52-53 0.06802062181596824 1102 54-55 -6.01430044021356E-4 1102 56-57 -0.04798513246878855 1102 58-59 0.182748595364572 1102 60-61 0.15683883329971593 1102 62-63 0.10238822406513748 1102 64-65 0.10311475491945998 1102 66-67 0.11684915452752165 1102 68-69 0.003058355636774479 1102 70-71 -0.09944368922984381 1102 72-73 -0.1437047840011516 1102 74-75 -0.148328113965988 1102 76-77 -0.05766982170734636 1102 78-79 0.034310685269311136 1102 80-81 0.13854185328190027 1102 82-83 0.2906371372668559 1102 84-85 0.26732132131665054 1102 86-87 0.19466889507756235 1102 88-89 0.4203523013356971 1102 90-91 0.15348119712766106 1102 92-93 0.1629194747209155 1102 94-95 0.42957507448321763 1102 96-97 0.07642459516051048 1102 98-99 0.18292483690172645 1102 100-101 0.2743864649862324 2305 1 0.0710852937408788 2305 2 0.04617983984670104 2305 3 0.04915166048223085 2305 4 0.09379034507281148 2305 5 0.09376042154881503 2305 6 0.09807883779061655 2305 7 0.06242241930819148 2305 8 0.03509710005543809 2305 9 0.06700355493759247 2305 10-11 0.006052740258162714 2305 12-13 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#Quality Count 7 5.0 8 53.0 9 160.0 10 127.0 11 55.0 12 42.0 13 121.0 14 351.0 15 816.0 16 1676.0 17 2555.0 18 3282.0 19 3759.0 20 4409.0 21 5511.0 22 6855.0 23 8596.0 24 10674.0 25 13412.0 26 16908.0 27 22328.0 28 29159.0 29 36577.0 30 41994.0 31 52823.0 32 70446.0 33 100602.0 34 155360.0 35 278062.0 36 587344.0 37 972244.0 38 236783.0 39 108.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.909667045914 24.353997611682072 25.093962445111 13.642372897292926 2 32.450697413672366 20.99709484503024 33.760101111558775 12.792106629738619 3 38.56728798991584 17.449394278358973 27.977419535316145 16.00589819640904 4 31.727987873716597 17.11650741405354 34.820400458791745 16.33510425343812 5 30.44814183854968 16.046503506875386 37.33749324589957 16.167861408675364 6 25.33203514422703 16.88767297349764 41.67333471763448 16.106957164640843 7 34.33741476879104 17.796467929334554 30.15139323151836 17.71472407035604 8 30.79862285816633 27.688864173397608 24.465970786239247 17.04654218219681 9 29.164947241980222 26.493346155015946 25.426207674460432 18.9154989285434 10-11 24.639392635985434 27.431392652507032 30.171856545209756 17.75735816629778 12-13 24.362523688634376 26.009566697469243 29.649684195348673 19.97822541854771 14-15 23.204967563420954 26.910870656583047 30.793647634778804 19.090514145217195 16-17 22.276124110647004 27.876719941483312 31.593885036340126 18.253270911529558 18-19 20.985041662332904 28.12645853836573 31.91680900812069 18.971690791180677 20-21 21.546134213879036 28.079034333547238 31.541057608580964 18.833773843992766 22-23 20.85873606436512 28.274697011825893 31.8392869580988 19.027279965710186 24-25 21.60185671581937 27.052373519495553 31.880105001620233 19.465664763064844 26-27 21.367120044067338 27.3648550970882 31.7277880682503 19.54023679059416 28-29 21.07827547117243 28.59931127888774 30.991680300030378 19.33073294990945 30-31 20.159755361694987 28.9056160697087 30.916638911804124 20.017989656792192 32-33 19.64732612720726 29.77179307426375 31.378039251320878 19.20284154720811 34-35 20.162064616323914 29.487660882766086 31.64144447444181 18.70883002646819 36-37 20.469245121698908 30.220102276571804 31.357374348098567 17.953278253630728 38-39 21.0347621754728 30.26501756169875 30.329339113887073 18.37088114894138 40-41 21.165130498994298 29.519390522166834 29.887068159957703 19.428410818881165 42-43 20.512524445085297 29.703951617506135 30.852983276707853 18.930540660700714 44-45 19.890671766347886 29.234375686446207 31.808767211142495 19.06618533606341 46-47 20.54560703518739 29.046320778965484 31.190236983962684 19.21783520188444 48-49 20.553536310917025 30.610795321193834 29.813833112966755 19.021835254922387 50-51 20.74516062409643 29.7415181840371 29.755355701169712 19.75796549069676 52-53 19.945170213780592 29.52767229496187 29.822135507900228 20.70502198335731 54-55 21.17944721919712 29.34901798790792 29.939595929250068 19.5319388636449 56-57 20.999915498511832 29.467959852404068 29.34135784502427 20.190766804059827 58-59 20.569990959797575 29.502345682768144 30.28080964458521 19.64685371284907 60-61 20.422272205708143 29.84111748203402 30.368253614179686 19.368356698078152 62-63 20.381591966010625 29.833657819490572 29.842387967790447 19.94236224670836 64-65 20.907847517128562 29.655412114447778 29.586556126549308 19.850184241874356 66-67 21.12129972143675 29.96791789746348 29.129805366324195 19.780977014775573 68-69 21.880624100519483 30.055944308072263 28.649881621603758 19.413549969804496 70-71 20.983267071748827 31.040227993941798 28.825742550454965 19.15076238385441 72-73 20.255562973792966 31.191340757204845 28.886015495665024 19.667080773337165 74-75 21.082387373073804 30.10367814589536 28.76267571723582 20.05125876379502 76-77 21.312674988679206 29.195460112582506 29.205358177538983 20.286506721199306 78-79 21.12704936887391 29.25947909681773 29.681882256269432 19.93158927803892 80-81 19.741138302732352 29.79733559957647 30.07172201368421 20.38980408400697 82-83 19.139723817648754 29.698896201816783 30.10604287825627 21.055337102278187 84-85 19.049772090406766 29.22515244669529 30.626388994246117 21.098686468651824 86-87 19.195494335620317 29.284489920393717 31.63021989000242 19.889795853983543 88-89 19.495746465215134 29.954510782469374 31.005341002002172 19.54440175031332 90-91 19.961430761380957 29.740661401033506 30.013752192735772 20.284155644849765 92-93 19.148393068482534 30.655298834773436 30.201544732382185 19.99476336436184 94-95 18.96297061405774 30.859004756024845 30.356415469052973 19.821609160864444 96-97 19.534145964943384 31.51132877775854 29.482635642784143 19.471889614513938 98-99 19.998474932930858 30.68720423653114 28.90640670909308 20.40791412144492 100-101 19.56141121754355 30.750356214430198 28.5469530803441 21.141279487682155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1542.0 1 962.5 2 428.5 3 697.0 4 788.5 5 572.0 6 493.5 7 536.0 8 573.0 9 557.5 10 529.0 11 501.0 12 467.5 13 460.0 14 482.0 15 501.5 16 532.5 17 544.0 18 555.5 19 598.0 20 677.5 21 842.0 22 1044.5 23 1361.0 24 1806.5 25 2426.0 26 3671.0 27 5653.5 28 7739.5 29 9755.5 30 12165.0 31 15584.0 32 23384.0 33 35501.5 34 51885.5 35 78778.5 36 125369.0 37 187257.5 38 236125.0 39 242749.5 40 222615.0 41 195941.0 42 169966.0 43 157652.0 44 140777.5 45 120679.0 46 115603.0 47 103797.0 48 78550.0 49 59960.0 50 48609.0 51 41103.0 52 35210.5 53 28862.5 54 22858.0 55 16640.5 56 11644.0 57 8673.0 58 6958.5 59 5496.0 60 4214.5 61 3298.0 62 2487.5 63 1779.0 64 1277.5 65 907.5 66 618.0 67 451.5 68 341.0 69 242.5 70 178.0 71 130.0 72 100.5 73 77.0 74 47.5 75 28.0 76 23.5 77 18.5 78 13.0 79 13.0 80 8.0 81 5.5 82 5.5 83 2.5 84 2.0 85 1.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16604104014836304 2 0.0 3 3.7548855754944156E-4 4 0.02068941952097423 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.195561199565785E-4 12-13 0.0 14-15 0.0 16-17 9.387213938736039E-4 18-19 0.0 20-21 0.0 22-23 5.820072642016344E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.76270249628548E-4 38-39 5.632328363241623E-4 40-41 0.004900125676020212 42-43 0.0031916527391702533 44-45 9.011725381186598E-4 46-47 0.004749930253000435 48-49 0.007885259708538272 50-51 0.00551968179597679 52-53 0.0017272473647274309 54-55 0.011152010159218413 56-57 0.019600502704080848 58-59 0.02425656081769392 60-61 0.011921761702194767 62-63 4.505862690593299E-4 64-65 0.014756700311693053 66-67 0.03623464580352111 68-69 0.020614321809464337 70-71 0.038337381725797974 72-73 0.03152226440627561 74-75 0.030902708286319035 76-77 0.03982056152811827 78-79 0.017141052652132005 80-81 0.013010678519088147 82-83 0.0010889168168933805 84-85 0.0112271078707283 86-87 0.022266471462681882 88-89 0.02207872718390716 90-91 0.026753559725397707 92-93 0.04440152193022146 94-95 0.02945707733975369 96-97 0.030714964007544317 98-99 0.038262284014288085 100-101 0.017835706483598474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2663197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.88423985021125 #Duplication Level Percentage of deduplicated Percentage of total 1 85.04930941981334 32.22028437155039 2 6.664338454391894 5.049467928983373 3 2.315649861905355 2.6317990433259313 4 1.1847849250301592 1.795387050830284 5 0.8302001998084393 1.5725751746618106 6 0.5481443956127847 1.2459620253566288 7 0.4123515589944865 1.0935137754488964 8 0.3239603784676518 0.9818394143866985 9 0.27938402342720187 0.9525826218449839 >10 1.929545679157368 14.518185971233455 >50 0.21324045714468293 5.64091794286422 >100 0.2087305084316054 17.98471744005638 >500 0.032755658702785906 8.202848004031985 >1k 0.007008594274070236 4.410801021345184 >5k 4.965706986942391E-4 1.0258294541642907 >10k+ 9.931413949859951E-5 0.6732887599154646 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 17895 0.6719367737347256 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5897 0.22142560238690565 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5505 0.20670645093096757 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5435 0.20407803102812147 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5220 0.1960050270408085 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5208 0.19555444077174913 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4223 0.15856881785312915 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 4092 0.15364991774923148 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3774 0.14170938161915922 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3763 0.14129634420585482 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3474 0.130444724892676 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 3044 0.11429871691804999 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3033 0.11388567950474562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.0039084603955324E-4 0.0 0.0 2 3.0039084603955324E-4 0.0 0.0 3 3.0039084603955324E-4 0.0 0.0 4 3.3793970179449734E-4 0.0 0.0 5 3.754885575494415E-4 0.0 0.0 6 4.88135124814274E-4 0.0 0.0 7 6.007816920791065E-4 0.0 0.0 8 8.260748266087713E-4 0.0 0.0 9 9.76270249628548E-4 0.0 3.7548855754944155E-5 10-11 0.0016521496532175427 0.0 3.7548855754944155E-5 12-13 0.0025533221913362023 0.0 3.7548855754944155E-5 14-15 0.0036046901524746386 0.0 3.7548855754944155E-5 16-17 0.004449539406960882 0.0 7.509771150988831E-5 18-19 0.005669877218996567 0.0 7.509771150988831E-5 20-21 0.006740019608012475 0.0 7.509771150988831E-5 22-23 0.007922808564293216 0.0 7.509771150988831E-5 24-25 0.009650055929020646 0.0 7.509771150988831E-5 26-27 0.011414852149503022 0.0 7.509771150988831E-5 28-29 0.01348003921602495 0.0 7.509771150988831E-5 30-31 0.020407803102812146 0.0 7.509771150988831E-5 32-33 0.02262318559235385 0.0 7.509771150988831E-5 34-35 0.02504508678854775 0.0 7.509771150988831E-5 36-37 0.027053950571437262 0.0 7.509771150988831E-5 38-39 0.029719919330038296 0.0 7.509771150988831E-5 40-41 0.03232956480500691 0.0 7.509771150988831E-5 42-43 0.03484533814058817 0.0 7.509771150988831E-5 44-45 0.0378492466009837 0.0 7.509771150988831E-5 46-47 0.04090947834501166 0.0 7.509771150988831E-5 48-49 0.04411990551205938 0.0 7.509771150988831E-5 50-51 0.047705821236656544 0.0 7.509771150988831E-5 52-53 0.05288756333083884 0.0 7.509771150988831E-5 54-55 0.05765626801171675 0.0 7.509771150988831E-5 56-57 0.0630633032404287 0.0 7.509771150988831E-5 58-59 0.06957802971391151 0.0 7.509771150988831E-5 60-61 0.0763743726055564 0.0 7.509771150988831E-5 62-63 0.08420330903046226 0.0 7.509771150988831E-5 64-65 0.09293341799348678 0.0 7.509771150988831E-5 66-67 0.10224553422071292 0.0 7.509771150988831E-5 68-69 0.11366038637021594 0.0 7.509771150988831E-5 70-71 0.12644577175477445 0.0 7.509771150988831E-5 72-73 0.14142776520099715 0.0 7.509771150988831E-5 74-75 0.15903817855006597 0.0 1.1264656726483246E-4 76-77 0.17820686941296493 0.0 1.1264656726483246E-4 78-79 0.20206916724523194 0.0 1.1264656726483246E-4 80-81 0.22876640368699724 0.0 1.1264656726483246E-4 82-83 0.2612837127707789 0.0 1.1264656726483246E-4 84-85 0.2983632078287862 0.0 1.1264656726483246E-4 86-87 0.34056812169734346 0.0 1.1264656726483246E-4 88-89 0.38998241587085 0.0 1.1264656726483246E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACCG 25 0.0050224415 56.998737 2 CGGAAGA 3405 0.0 55.241066 4 TCGGAAG 3500 0.0 54.1488 3 GAAGAGC 3600 0.0 52.23315 6 GATCGGA 3835 0.0 49.745842 1 ATCGGAA 3900 0.0 48.59508 2 GTCCCGA 40 5.347767E-4 47.574894 1 CGCGGCT 40 5.3900556E-4 47.498947 3 CGCGGAG 115 1.8189894E-12 45.50642 1 GCGCAAC 65 2.591063E-6 43.84518 2 GATTCGC 55 4.974542E-5 43.16789 7 GCCGTCT 2100 0.0 42.30026 50-51 TATGCCG 2155 0.0 42.202705 48-49 CGTATGC 2140 0.0 42.166027 46-47 TGCCGTC 2095 0.0 42.061096 50-51 ATGCCGT 2120 0.0 41.779354 48-49 CTCGTAT 2135 0.0 41.709045 44-45 GGAAGAG 4510 0.0 41.588577 5 TCGTATG 2215 0.0 41.381905 44-45 AATCTCG 2175 0.0 41.157253 40-41 >>END_MODULE