FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34311-1.34100000035764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34311-1.34100000035764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2663197
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT178950.6719367737347256TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58970.22142560238690565No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG55050.20670645093096757No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC54350.20407803102812147No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG52200.1960050270408085No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC52080.19555444077174913No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT42230.15856881785312915No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT40920.15364991774923148No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA37740.14170938161915922No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA37630.14129634420585482No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT34740.130444724892676No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT30440.11429871691804999No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG30330.11388567950474562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG250.005022441556.9987372
CGGAAGA34050.055.2410664
TCGGAAG35000.054.14883
GAAGAGC36000.052.233156
GATCGGA38350.049.7458421
ATCGGAA39000.048.595082
GTCCCGA405.347767E-447.5748941
CGCGGCT405.3900556E-447.4989473
CGCGGAG1151.8189894E-1245.506421
GCGCAAC652.591063E-643.845182
GATTCGC554.974542E-543.167897
GCCGTCT21000.042.3002650-51
TATGCCG21550.042.20270548-49
CGTATGC21400.042.16602746-47
TGCCGTC20950.042.06109650-51
ATGCCGT21200.041.77935448-49
CTCGTAT21350.041.70904544-45
GGAAGAG45100.041.5885775
TCGTATG22150.041.38190544-45
AATCTCG21750.041.15725340-41
Produced by FastQC (version 0.11.2)