##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34310-1.34100000035813.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5805783 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.659430950140575 33.0 33.0 33.0 33.0 33.0 2 32.706790625829456 33.0 33.0 33.0 33.0 33.0 3 32.73697535715682 33.0 33.0 33.0 33.0 33.0 4 36.660643706456135 37.0 37.0 37.0 37.0 37.0 5 36.665584469829476 37.0 37.0 37.0 37.0 37.0 6 36.55240542059529 37.0 37.0 37.0 37.0 37.0 7 36.51164209892102 37.0 37.0 37.0 37.0 37.0 8 36.44309044964305 37.0 37.0 37.0 37.0 37.0 9 36.450548530663305 37.0 37.0 37.0 37.0 37.0 10-11 36.40337289561115 37.0 37.0 37.0 37.0 37.0 12-13 36.37248136900742 37.0 37.0 37.0 37.0 37.0 14-15 38.21929996350191 40.0 37.0 40.0 37.0 40.0 16-17 38.173857772500284 40.0 37.0 40.0 37.0 40.0 18-19 38.21506367013717 40.0 37.0 40.0 37.0 40.0 20-21 38.153852546676305 40.0 37.0 40.0 37.0 40.0 22-23 38.06282382583021 40.0 37.0 40.0 37.0 40.0 24-25 38.032628846100515 40.0 37.0 40.0 35.0 40.0 26-27 37.91431655299552 40.0 37.0 40.0 33.0 40.0 28-29 37.81626345662592 40.0 37.0 40.0 33.0 40.0 30-31 37.77431106191878 40.0 37.0 40.0 33.0 40.0 32-33 37.664679510067806 40.0 37.0 40.0 33.0 40.0 34-35 37.56236066694191 40.0 37.0 40.0 33.0 40.0 36-37 37.439071181268744 40.0 37.0 40.0 33.0 40.0 38-39 37.33418050588526 40.0 37.0 40.0 33.0 40.0 40-41 37.24300624050193 40.0 37.0 40.0 33.0 40.0 42-43 37.08795153039651 38.5 37.0 40.0 33.0 40.0 44-45 36.98691812973375 37.0 37.0 40.0 33.0 40.0 46-47 36.80560210397116 37.0 37.0 40.0 33.0 40.0 48-49 36.69621444687134 37.0 37.0 40.0 33.0 40.0 50-51 36.519463007832016 37.0 37.0 40.0 33.0 40.0 52-53 36.347380792564934 37.0 37.0 40.0 33.0 40.0 54-55 36.20182514572109 37.0 37.0 40.0 33.0 40.0 56-57 35.95513370031226 37.0 37.0 40.0 33.0 40.0 58-59 35.66239248004963 37.0 35.0 40.0 27.0 40.0 60-61 35.42215976725275 37.0 33.0 40.0 27.0 40.0 62-63 35.16147813309591 37.0 33.0 37.0 27.0 40.0 64-65 34.872498507091976 37.0 33.0 37.0 27.0 40.0 66-67 34.54375921387348 37.0 33.0 37.0 27.0 40.0 68-69 34.211835509525585 37.0 33.0 37.0 27.0 40.0 70-71 33.887762425843334 37.0 33.0 37.0 27.0 37.0 72-73 33.569288242430005 37.0 33.0 37.0 27.0 37.0 74-75 33.17175194112491 37.0 33.0 37.0 27.0 37.0 76-77 30.350338102543617 33.0 30.0 33.0 24.5 37.0 78-79 32.90349863231195 33.0 33.0 37.0 27.0 37.0 80-81 33.393608493462466 37.0 33.0 37.0 27.0 37.0 82-83 33.27030720920847 37.0 33.0 37.0 27.0 37.0 84-85 33.13139597191283 37.0 33.0 37.0 27.0 37.0 86-87 33.02274499408607 37.0 33.0 37.0 27.0 37.0 88-89 32.77825273524691 35.0 33.0 37.0 27.0 37.0 90-91 32.76638629104808 33.0 33.0 37.0 27.0 37.0 92-93 32.57150783968329 33.0 33.0 37.0 27.0 37.0 94-95 32.357416217588565 33.0 33.0 37.0 22.0 37.0 96-97 32.23772943287753 33.0 33.0 37.0 22.0 37.0 98-99 32.099107045509626 33.0 33.0 37.0 22.0 37.0 100-101 30.394796102437862 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.14765666082081452 2306 2 0.11819951751988356 2306 3 0.12079524664876828 2306 4 0.17453446364156378 2306 5 0.15546292254826 2306 6 0.1403624628018605 2306 7 0.20041312323905203 2306 8 0.21812111264662093 2306 9 0.1707038072538012 2306 10-11 0.2318555547326966 2306 12-13 0.2457123340583891 2306 14-15 0.4583616196747755 2306 16-17 0.4285042111571755 2306 18-19 0.47738991005988396 2306 20-21 0.4279905955480885 2306 22-23 0.4369351230137468 2306 24-25 0.45992119480914795 2306 26-27 0.49230283168454747 2306 28-29 0.4459643088789207 2306 30-31 0.47369440642334126 2306 32-33 0.4578620047369384 2306 34-35 0.5147763971442814 2306 36-37 0.44519510024969833 2306 38-39 0.4961527925539144 2306 40-41 0.6342599286241395 2306 42-43 0.5361148865505143 2306 44-45 0.46888457947625284 2306 46-47 0.6655095154334134 2306 48-49 0.5783620470134636 2306 50-51 0.6130099107328064 2306 52-53 0.5264062939933893 2306 54-55 0.594298818948154 2306 56-57 0.5711209218449653 2306 58-59 0.6298677352929047 2306 60-61 0.5655269753907035 2306 62-63 0.48001571056256154 2306 64-65 0.3579716905040229 2306 66-67 0.4442610974966854 2306 68-69 0.4552427904185876 2306 70-71 0.34060251970252153 2306 72-73 0.3810225683257755 2306 74-75 0.4028489411649261 2306 76-77 -0.11603632553389787 2306 78-79 0.31115881256643974 2306 80-81 0.381473629311742 2306 82-83 0.5690185429625956 2306 84-85 0.5048233545840404 2306 86-87 0.415617776555969 2306 88-89 0.7418055088848305 2306 90-91 0.5183121361100831 2306 92-93 0.5541810193942638 2306 94-95 0.4683780724061464 2306 96-97 0.4605030566462105 2306 98-99 0.4244246701666867 2306 100-101 0.3320167701083001 2307 1 0.16068705379913695 2307 2 0.14399228116803897 2307 3 0.10011995567548126 2307 4 0.1553433148904375 2307 5 0.15379968130689292 2307 6 0.15865811645691963 2307 7 0.18296161461161375 2307 8 0.1902004522596954 2307 9 0.14125767410182277 2307 10-11 0.21127670169283874 2307 12-13 0.23691331196408782 2307 14-15 0.45751371732507096 2307 16-17 0.41547444439920156 2307 18-19 0.37634236866359316 2307 20-21 0.3519831009429737 2307 22-23 0.3701725193899321 2307 24-25 0.3923526455988693 2307 26-27 0.4198961040741196 2307 28-29 0.45024890849241217 2307 30-31 0.39408050884042467 2307 32-33 0.43632628700700593 2307 34-35 0.5067488785173495 2307 36-37 0.33695917549132304 2307 38-39 0.46985153798407 2307 40-41 0.45250448269889176 2307 42-43 0.34973918690583616 2307 44-45 0.4062274764467162 2307 46-47 0.40493421932512064 2307 48-49 0.3814000924044123 2307 50-51 0.433211403391617 2307 52-53 0.3546459510250344 2307 54-55 0.42618370461909905 2307 56-57 0.4225576591868574 2307 58-59 0.4012215360120521 2307 60-61 0.44695653624741993 2307 62-63 0.3642094080305043 2307 64-65 0.3671345466198801 2307 66-67 0.3458017247941285 2307 68-69 0.27240899305598276 2307 70-71 0.22561280850282373 2307 72-73 0.17058184697003753 2307 74-75 0.13610850574139022 2307 76-77 -0.18354287892982768 2307 78-79 0.15034480126190175 2307 80-81 0.33740086751719645 2307 82-83 0.43772706369237113 2307 84-85 0.4884207650376666 2307 86-87 0.37165773442386296 2307 88-89 0.6378692130282815 2307 90-91 0.44870711833168997 2307 92-93 0.4482671196821997 2307 94-95 0.48988874132219706 2307 96-97 0.5525374085895578 2307 98-99 0.6062270826179557 2307 100-101 0.37162708571083414 2201 1 0.12043641125319482 2201 2 0.12060722887104447 2201 3 0.09586589867368644 2201 4 0.15374351854334378 2201 5 0.14831249660981882 2201 6 0.12814988334440613 2201 7 0.14987502359078775 2201 8 0.1898769897865904 2201 9 0.19557951809482432 2201 10-11 0.21276848259314107 2201 12-13 0.1464127158355879 2201 14-15 0.3383300572012189 2201 16-17 0.33202054247255575 2201 18-19 0.29833127752073807 2201 20-21 0.3476362090436709 2201 22-23 0.3441840028915948 2201 24-25 0.3617825225546767 2201 26-27 0.40335339243098645 2201 28-29 0.4626514172533476 2201 30-31 0.41750176090877034 2201 32-33 0.44145380945962387 2201 34-35 0.41494100354902486 2201 36-37 0.3990248512184351 2201 38-39 0.44983831922885287 2201 40-41 0.41198526120416545 2201 42-43 0.43831710808142077 2201 44-45 0.4488147558470672 2201 46-47 0.4831998766020362 2201 48-49 0.3803724859916855 2201 50-51 0.31656581433376374 2201 52-53 0.32367938209775104 2201 54-55 0.39307434277436215 2201 56-57 0.362443267909363 2201 58-59 0.4336559472811601 2201 60-61 0.3531757737401122 2201 62-63 0.47931008426558463 2201 64-65 0.5076550526226242 2201 66-67 0.41777329279306485 2201 68-69 0.3603861148004839 2201 70-71 0.18423179890287855 2201 72-73 0.29537121684879253 2201 74-75 0.33233637279247574 2201 76-77 -0.05345549735270794 2201 78-79 0.2678515945577047 2201 80-81 0.37344474631102287 2201 82-83 0.5076463382022425 2201 84-85 0.4396631762226946 2201 86-87 0.39404670914124296 2201 88-89 0.5535272489688126 2201 90-91 0.2876712677129589 2201 92-93 0.3655267035716676 2201 94-95 0.4878084222036989 2201 96-97 0.35459355560266914 2201 98-99 0.335434109110345 2201 100-101 0.34600056692606884 1101 1 0.12435987696891004 1101 2 0.11660520803692975 1101 3 0.08012099717456067 1101 4 0.13413994820581365 1101 5 0.11378715222847546 1101 6 0.0770381656871848 1101 7 0.14228905492555555 1101 8 0.09544781994850382 1101 9 0.14082319799528875 1101 10-11 0.10763658470295212 1101 12-13 0.22877834922876872 1101 14-15 0.3387659567423924 1101 16-17 0.2990412989243296 1101 18-19 0.27837849935462344 1101 20-21 0.26265318123944326 1101 22-23 0.28802863240657217 1101 24-25 0.2623990544867425 1101 26-27 0.336985782585117 1101 28-29 0.34140129612904246 1101 30-31 0.39467136525578894 1101 32-33 0.448575326535007 1101 34-35 0.3185521719861484 1101 36-37 0.4092320606847011 1101 38-39 0.3515433055556798 1101 40-41 0.42974189863087275 1101 42-43 0.4595615335897847 1101 44-45 0.37212040256946466 1101 46-47 0.4271233571115616 1101 48-49 0.30516002842217205 1101 50-51 0.3187319236437318 1101 52-53 0.17700499306351247 1101 54-55 0.2399548095485713 1101 56-57 0.37046506214673514 1101 58-59 0.3554112769352784 1101 60-61 0.2728820131024392 1101 62-63 0.37422412114803194 1101 64-65 0.28740802562317924 1101 66-67 0.31256158231084186 1101 68-69 0.33668347625093276 1101 70-71 0.19456564336226734 1101 72-73 0.363054218098128 1101 74-75 0.1541193668668157 1101 76-77 -0.15368768616311712 1101 78-79 0.21746211058790976 1101 80-81 0.35261802396045994 1101 82-83 0.35143179248679246 1101 84-85 0.3457594084618023 1101 86-87 0.42704030268286175 1101 88-89 0.6094763937757861 1101 90-91 0.4230509544836778 1101 92-93 0.4710404296362114 1101 94-95 0.4822495010569128 1101 96-97 0.5429422486067494 1101 98-99 0.46672892972434 1101 100-101 0.3654652973791954 2304 1 0.11789735653137257 2304 2 0.12015833910133722 2304 3 0.08042485896982754 2304 4 0.07197052342579724 2304 5 0.09820157431258991 2304 6 0.0857159084655521 2304 7 0.07341589757884037 2304 8 0.07068352021678237 2304 9 0.1083248297243955 2304 10-11 0.09973585058857992 2304 12-13 0.12094113794658767 2304 14-15 0.23326424642833388 2304 16-17 0.23380837095834295 2304 18-19 0.2520956546668529 2304 20-21 0.2449866302672845 2304 22-23 0.2834273746108309 2304 24-25 0.1889371911066675 2304 26-27 0.11179908876298583 2304 28-29 0.16181548245831578 2304 30-31 0.16567668502011657 2304 32-33 0.13574502417942114 2304 34-35 0.1976943081432978 2304 36-37 0.21408118850093416 2304 38-39 0.25254231669509863 2304 40-41 0.18159191925942508 2304 42-43 0.26375022041349183 2304 44-45 0.28145596684212393 2304 46-47 0.26349225231635387 2304 48-49 0.28682343163062995 2304 50-51 0.14169106165417844 2304 52-53 0.16727486686918525 2304 54-55 0.26696674791178765 2304 56-57 0.17409219403628384 2304 58-59 0.254012355731966 2304 60-61 0.2628424586790885 2304 62-63 0.13162998838749473 2304 64-65 0.14409833326142518 2304 66-67 0.17577902467642303 2304 68-69 0.1696289055539424 2304 70-71 0.18191990648649892 2304 72-73 0.07597958641049019 2304 74-75 0.00700605392191278 2304 76-77 -0.1169245564739434 2304 78-79 0.08399136274298513 2304 80-81 0.1158872014161858 2304 82-83 0.2485610407126302 2304 84-85 0.24270671070605232 2304 86-87 0.20653323331199402 2304 88-89 0.4149514725244501 2304 90-91 0.28354160833226416 2304 92-93 0.27631249727165397 2304 94-95 0.22326876964934428 2304 96-97 0.16157332065822771 2304 98-99 0.18553758192700798 2304 100-101 0.34519267310037804 2202 1 0.08169741684464071 2202 2 0.09226716543112445 2202 3 0.052885091140360885 2202 4 0.060423495870225 2202 5 0.10050438725883737 2202 6 0.10954830712759644 2202 7 0.1403543033401462 2202 8 0.10456139888118088 2202 9 0.08476099508078505 2202 10-11 0.07687836500388556 2202 12-13 0.10318391823829387 2202 14-15 0.10050131355906444 2202 16-17 0.08314699994989638 2202 18-19 0.06112974115198 2202 20-21 0.10041603033992885 2202 22-23 0.21276478231789042 2202 24-25 0.10780038471425968 2202 26-27 0.05517583368803258 2202 28-29 0.1448080879561502 2202 30-31 0.12934815474708472 2202 32-33 0.10885451332283935 2202 34-35 0.16885486041684317 2202 36-37 0.14753857377429824 2202 38-39 0.18442703139078276 2202 40-41 0.15818841278957763 2202 42-43 0.18892024179189804 2202 44-45 0.22852603023429907 2202 46-47 0.30083059515580857 2202 48-49 0.19553658510182004 2202 50-51 0.16597243058367184 2202 52-53 0.18917372316984427 2202 54-55 0.10078267306641209 2202 56-57 0.19619637632307985 2202 58-59 0.20258021191919084 2202 60-61 0.12558792599431 2202 62-63 0.20005456695541568 2202 64-65 0.17363480243703577 2202 66-67 0.1493957223626552 2202 68-69 0.09339217714192927 2202 70-71 0.05914447224871111 2202 72-73 0.13202154563539636 2202 74-75 0.143292096141991 2202 76-77 -0.16134528226474742 2202 78-79 0.2076076651915244 2202 80-81 0.26965269028767125 2202 82-83 0.3416795570851505 2202 84-85 0.4554363487513271 2202 86-87 0.3901102965614882 2202 88-89 0.4871436347236724 2202 90-91 0.3060419966533061 2202 92-93 0.46309126257278876 2202 94-95 0.4293961595035185 2202 96-97 0.3348451536876951 2202 98-99 0.32836929406116155 2202 100-101 0.27892856262037213 1102 1 0.09791349964191909 1102 2 0.06151924086300653 1102 3 0.07599338741268014 1102 4 0.08376362881590893 1102 5 0.06279560323878997 1102 6 0.04080896598946282 1102 7 0.07269283639458024 1102 8 0.12602398029543593 1102 9 0.07146485243737999 1102 10-11 0.05971417001568824 1102 12-13 0.0754403730259483 1102 14-15 0.19210166090269354 1102 16-17 0.17868527974038528 1102 18-19 0.1744006468299375 1102 20-21 0.12487494246753528 1102 22-23 0.130535880716117 1102 24-25 0.07263865618781296 1102 26-27 0.061809131279431995 1102 28-29 0.14824044045807483 1102 30-31 0.12811701651701668 1102 32-33 0.11426323679920358 1102 34-35 0.05266995269452224 1102 36-37 0.16379239576158255 1102 38-39 0.2355932707341566 1102 40-41 0.2722819433802286 1102 42-43 0.22758786952609 1102 44-45 0.11432793883570014 1102 46-47 0.2923912358635192 1102 48-49 0.1635387779097286 1102 50-51 0.0579564656542928 1102 52-53 0.10815410326251396 1102 54-55 0.1360426486879689 1102 56-57 0.16004843869826857 1102 58-59 0.3085739107264729 1102 60-61 0.17432762484988729 1102 62-63 0.14751537230257128 1102 64-65 0.10500272287352175 1102 66-67 0.16459935748729038 1102 68-69 0.1222297296034256 1102 70-71 -0.01847024997027802 1102 72-73 -0.061070823267868946 1102 74-75 -0.00951688900468639 1102 76-77 0.03256853108366187 1102 78-79 0.0706309677999144 1102 80-81 0.14607444272753156 1102 82-83 0.22299767431094608 1102 84-85 0.231427463050089 1102 86-87 0.12361949571262443 1102 88-89 0.31859577973160214 1102 90-91 0.11131723786665049 1102 92-93 0.2910090161787977 1102 94-95 0.3525250603004224 1102 96-97 0.19178167941070257 1102 98-99 0.1121489365404642 1102 100-101 0.0851366414899104 2305 1 0.08349754882296878 2305 2 0.0694103834514479 2305 3 0.0527353698373787 2305 4 0.11196610243597149 2305 5 0.08649424956227136 2305 6 0.03565292009103871 2305 7 0.05639122373414551 2305 8 0.03547427076207299 2305 9 0.0303944395684681 2305 10-11 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82-83 -1.3558417068772712 1213 84-85 -1.2470623050127898 1213 86-87 -1.0602411803965524 1213 88-89 -0.8052015407943642 1213 90-91 -0.7866165317749108 1213 92-93 -0.9153311718129373 1213 94-95 -0.8643836543768337 1213 96-97 -0.8101064963165747 1213 98-99 -0.919744093041988 1213 100-101 -0.8035126573078841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 19.0 9 70.0 10 79.0 11 69.0 12 83.0 13 165.0 14 542.0 15 1204.0 16 2621.0 17 4304.0 18 6298.0 19 8377.0 20 11099.0 21 14138.0 22 18124.0 23 23003.0 24 29374.0 25 36811.0 26 47070.0 27 61142.0 28 80137.0 29 104185.0 30 118016.0 31 141504.0 32 190212.0 33 269427.0 34 411799.0 35 719499.0 36 1379317.0 37 1819801.0 38 307209.0 39 85.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.94241384004291 24.74274809542237 22.90648285323732 15.408355211297398 2 31.669733436471876 21.953249027736653 32.50994740933307 13.867070126458394 3 39.51353247879309 18.760344229786455 24.84909416885572 16.87702912256473 4 31.266696092102304 18.254451458540284 32.78115848698131 17.6976939623761 5 34.31754166492272 16.79924998919181 31.35434100792262 17.528867337962854 6 28.05692186566394 17.778893906299977 36.41619054656366 17.747993681472423 7 34.53578957394722 18.61387172066197 27.499891056899646 19.35044764849117 8 30.10599948361832 25.95587881944606 24.935172396212536 19.002949300723092 9 31.634372142396643 23.656171785958932 24.827658904922902 19.881797166721526 10-11 26.171027107915968 27.967390304590715 28.37942091282482 17.482161674668493 12-13 25.877207260416036 26.226565822387784 27.964315924312018 19.931910992884163 14-15 25.4961906085708 26.71358023543078 27.73507036001862 20.055158795979803 16-17 24.871058108939533 27.081956816351333 28.542448511088036 19.504536563621095 18-19 23.410468493224773 27.18455891306995 29.219900227066702 20.185072366638572 20-21 23.35138085595001 27.86976364772848 28.982171741520478 19.79668375480103 22-23 23.227145334117957 28.434666524508906 28.447033560570446 19.89115458080269 24-25 23.475541541941887 26.77171192929533 29.48377505669778 20.268971472065008 26-27 22.673771995956447 26.646173306856287 29.375340070409106 21.30471462677816 28-29 22.394808762228973 27.88166557379082 28.356760836565886 21.366764827414322 30-31 20.985205613093015 28.47421269448066 28.790328539664678 21.75025315276165 32-33 20.99876967499474 28.082103654235784 28.87181625630858 22.047310414460892 34-35 22.024092185326253 28.076815823119812 29.029701936844692 20.869390054709243 36-37 21.34189176515331 29.321947353277228 29.32185261938774 20.014308262181725 38-39 21.293122313380113 28.559829210357996 28.798437959270817 21.348610516991076 40-41 21.74615188382471 27.829001207991798 27.775052394254214 22.649794513929283 42-43 22.38846486846716 28.440066566722994 28.31001979380783 20.861448771002017 44-45 21.277460078834274 28.64409577893263 28.252370268752898 21.826073873480194 46-47 22.041344287923508 28.39052753386333 28.43985970646668 21.12826847174648 48-49 22.140667041034632 28.28681007486211 28.694787902172823 20.87773498193043 50-51 21.77230448260876 28.29098915318128 27.568878444352844 22.367827919857113 52-53 21.127912541654183 28.412335864672063 27.856226207068087 22.603525386605675 54-55 22.062544604644586 28.454529058499418 28.288048117117693 21.194878219738307 56-57 21.991499341829513 28.27581727788404 27.543043798614477 22.189639581671972 58-59 21.3931811791752 27.923706650605467 28.169190126771394 22.51392204344794 60-61 21.0932276980485 27.84245523486033 28.93450638753889 22.12981067955228 62-63 20.82914531534645 27.903150144524837 28.809880619203433 22.457823920925282 64-65 21.906628124913865 28.43213389928613 28.266155150078554 21.395082825721452 66-67 22.17480735208272 28.68607779798658 27.76813571400511 21.370979135925587 68-69 21.789421375951747 27.845315964754857 28.692204717986424 21.67305794130697 70-71 21.369167666739322 29.244246602840064 28.431900436700786 20.95468529371983 72-73 22.357272814985322 29.504607103968244 27.69870504886218 20.439415032184257 74-75 23.37781957186597 28.44395624691921 27.46106772141223 20.717156459802585 76-77 23.399133971033407 27.194190036505685 27.90344606677578 21.50322992568513 78-79 23.0625199779391 27.39416354333939 28.63384446135945 20.909472017362063 80-81 22.175745479714745 28.457116850248077 28.27345792132166 21.093679748715516 82-83 21.34900967715877 28.436400709436686 28.56481688014244 21.649772733262104 84-85 21.569687861853176 27.908356424755382 28.756744400610074 21.765211312781364 86-87 20.785594829602303 28.538831173473717 29.27192438043563 21.40364961648835 88-89 20.718948680350515 29.153545313020462 29.123681214051857 21.003824792577163 90-91 21.436027656489475 28.22147933137067 28.43153723275225 21.910955779387603 92-93 21.353135794074717 28.561392559327214 27.65171680362836 22.433754842969705 94-95 21.103765013549204 29.589347604340325 27.150861300721864 22.156026081388607 96-97 21.085191850292937 30.398784659557364 26.452253436558593 22.063770053591103 98-99 21.340792538061407 29.042369441876897 27.337737787134568 22.279100232927128 100-101 21.974149771664013 29.086330453142157 27.04127240399923 21.89824737119461 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 190.0 1 124.5 2 150.0 3 331.0 4 322.5 5 193.0 6 182.5 7 218.0 8 213.5 9 199.0 10 198.5 11 188.0 12 193.5 13 202.0 14 199.5 15 208.5 16 224.0 17 243.0 18 267.5 19 312.5 20 368.5 21 455.5 22 602.0 23 815.0 24 1149.0 25 1563.0 26 2065.5 27 2845.5 28 3890.0 29 5154.5 30 6918.5 31 9807.5 32 16032.0 33 27980.0 34 47735.0 35 81507.5 36 127500.5 37 193881.5 38 280365.0 39 334957.0 40 354787.0 41 346404.5 42 356098.0 43 400849.5 44 398505.5 45 379018.0 46 392916.0 47 378702.0 48 313896.0 49 254738.0 50 206558.0 51 170911.5 52 153099.0 53 139947.0 54 111875.0 55 70560.0 56 45350.0 57 36068.0 58 31774.5 59 27511.0 60 22319.0 61 17517.0 62 12506.5 63 8425.0 64 5983.5 65 4416.0 66 3626.0 67 3054.5 68 2430.5 69 1810.5 70 1225.0 71 901.5 72 711.5 73 513.0 74 311.5 75 189.5 76 130.5 77 92.5 78 64.0 79 35.5 80 21.0 81 18.5 82 15.5 83 12.5 84 8.5 85 4.0 86 3.0 87 1.5 88 2.0 89 3.0 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1656279609485921 2 0.0 3 1.7224205589495852E-4 4 0.022064207360144188 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.306051397373963E-4 12-13 0.0 14-15 0.0 16-17 0.001154021774496222 18-19 0.0 20-21 0.0 22-23 4.564414481216401E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.009255599115572E-4 38-39 7.664771487325654E-4 40-41 0.004822777565058839 42-43 0.0030917449033145052 44-45 0.0010248402325750033 46-47 0.0041510335470685 48-49 0.007630323076146662 50-51 0.005201710088027747 52-53 0.0016104632226178622 54-55 0.010575662231950453 56-57 0.01818876110250762 58-59 0.02271872717254503 60-61 0.011230182044351295 62-63 5.77010887248111E-4 64-65 0.014175521200155087 66-67 0.03325994099331649 68-69 0.019368619185388087 70-71 0.03510293099139255 72-73 0.029332822118911438 74-75 0.028695526512100094 76-77 0.037316241409642764 78-79 0.01623381376809984 80-81 0.012556445874742478 82-83 8.612102794747926E-4 84-85 0.010282850736929024 86-87 0.02067765881018977 88-89 0.019885345353072962 90-91 0.024647838198568566 92-93 0.04127780869522681 94-95 0.02699894226153475 96-97 0.028385490811489167 98-99 0.035077094683008306 100-101 0.016535237365916017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5805783.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.808299268054544 #Duplication Level Percentage of deduplicated Percentage of total 1 82.0608659023376 24.46094849012572 2 8.168216532909593 4.869612857984801 3 2.5985604257835138 2.3237600051363465 4 1.5708100412260366 1.872927032085232 5 0.8569006771165417 1.2771375913244227 6 0.6051782115699735 1.0823599944590279 7 0.4796067155358765 1.0007382355363479 8 0.3334099239880247 0.7950706234539486 9 0.2850579216217773 0.7647382652788417 >10 2.3503217871613216 13.962276059174542 >50 0.32574958681927285 6.7780783050429925 >100 0.3050327011175964 18.913184377988117 >500 0.03757346614815902 7.789597596827982 >1k 0.021901884220375707 11.077701569668328 >5k 5.234395490725601E-4 1.0256850783290958 >10k+ 2.907828952211429E-4 2.0061839175841434 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 42100 0.7251390553177754 TruSeq Adapter, Index 7 (97% over 38bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 24594 0.423612112268061 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24344 0.41930606087068706 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 13360 0.23011538667566458 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11329 0.19513302512339853 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8299 0.14294368218722608 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 8225 0.1416690909736034 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7569 0.13037001210689408 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 7004 0.12063833594882895 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6302 0.10854694362500286 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6121 0.10542936241330413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.4782934532689215E-4 0.0 0.0 2 5.683987844533632E-4 0.0 0.0 3 5.856229900428589E-4 0.0 0.0 4 5.856229900428589E-4 0.0 0.0 5 6.545198124008425E-4 0.0 0.0 6 7.923134571168093E-4 0.0 0.0 7 9.128828962432802E-4 0.0 0.0 8 0.0010851249521382387 0.0 0.0 9 0.0012745912136226932 0.0 0.0 10-11 0.002187474109865973 0.0 0.0 12-13 0.0029625633613932865 0.0 0.0 14-15 0.004176869855452745 0.0 0.0 16-17 0.005795945180865354 0.0 0.0 18-19 0.007423632609072713 0.0 0.0 20-21 0.009456088868633222 0.0 0.0 22-23 0.01196221078190487 0.0 0.0 24-25 0.0149506104516824 0.0 0.0 26-27 0.018007906943817913 0.0 0.0 28-29 0.021581929603638302 0.0 0.0 30-31 0.033931685011306834 0.0 0.0 32-33 0.0386166689316497 0.0 0.0 34-35 0.04282798719828144 0.0 0.0 36-37 0.047521583221419056 0.0 0.0 38-39 0.05313667424359471 0.0 0.0 40-41 0.059113473583149764 0.0 0.0 42-43 0.06602038002453761 0.0 0.0 44-45 0.07361625468950528 0.0 0.0 46-47 0.08239198743735342 0.0 0.0 48-49 0.09115049597961206 0.0 0.0 50-51 0.1009338447544457 0.0 0.0 52-53 0.11249989880779215 0.0 8.612102794747927E-6 54-55 0.12661513528838403 0.0 1.7224205589495853E-5 56-57 0.14146240050652945 0.0 1.7224205589495853E-5 58-59 0.15855742455410407 0.0 1.7224205589495853E-5 60-61 0.17765906855285496 0.0 1.7224205589495853E-5 62-63 0.19899124717544558 0.0 1.7224205589495853E-5 64-65 0.22356157644886143 0.0 1.7224205589495853E-5 66-67 0.2515681347373817 0.0 2.583630838424378E-5 68-69 0.28155547666869396 0.0 3.4448411178991706E-5 70-71 0.31518573808218464 0.0 3.4448411178991706E-5 72-73 0.3538540796306028 0.0 5.1672616768487556E-5 74-75 0.39650121267019456 0.0 5.1672616768487556E-5 76-77 0.445478241263926 0.0 6.028471956323549E-5 78-79 0.500277051346907 0.0 9.473313074222719E-5 80-81 0.5593216281076989 0.0 1.0334523353697511E-4 82-83 0.6269007987380858 0.0 1.0334523353697511E-4 84-85 0.7039446703399007 0.0 1.0334523353697511E-4 86-87 0.789893456231485 0.0 1.0334523353697511E-4 88-89 0.8818965503877771 0.0 1.0334523353697511E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTCG 40 5.346927E-4 47.577354 1 TTGTGTT 64805 0.0 46.47244 1 CGACGCC 95 3.8016879E-10 45.073284 1 TCGAGCG 45 9.551503E-4 42.290985 1 CGGAAGA 11480 0.0 41.789623 4 GAAGAGC 11590 0.0 41.42649 6 GCCGTCT 5755 0.0 41.394676 50-51 TATGCCG 5755 0.0 41.26429 48-49 TCGGAAG 11700 0.0 41.247425 3 ATGCCGT 5770 0.0 41.198174 48-49 TGCCGTC 5750 0.0 41.18283 50-51 CGTATGC 5790 0.0 41.09688 46-47 TCGTATG 5815 0.0 41.00205 44-45 TCTCGTA 5730 0.0 40.86428 42-43 CTCGTAT 5740 0.0 40.83446 44-45 CATCTCG 5845 0.0 39.814617 40-41 ATCTCGT 5870 0.0 39.7683 42-43 GTCGCGC 85 3.5979065E-7 39.181355 1 CTTGAAA 5910 0.0 39.02569 62-63 GATCGGA 12500 0.0 38.70894 1 >>END_MODULE