FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34310-1.34100000035813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34310-1.34100000035813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5805783
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT421000.7251390553177754TruSeq Adapter, Index 7 (97% over 38bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA245940.423612112268061No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC243440.41930606087068706No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT133600.23011538667566458No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC113290.19513302512339853No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC82990.14294368218722608No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT82250.1416690909736034No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC75690.13037001210689408No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA70040.12063833594882895No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT63020.10854694362500286No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG61210.10542936241330413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG405.346927E-447.5773541
TTGTGTT648050.046.472441
CGACGCC953.8016879E-1045.0732841
TCGAGCG459.551503E-442.2909851
CGGAAGA114800.041.7896234
GAAGAGC115900.041.426496
GCCGTCT57550.041.39467650-51
TATGCCG57550.041.2642948-49
TCGGAAG117000.041.2474253
ATGCCGT57700.041.19817448-49
TGCCGTC57500.041.1828350-51
CGTATGC57900.041.0968846-47
TCGTATG58150.041.0020544-45
TCTCGTA57300.040.8642842-43
CTCGTAT57400.040.8344644-45
CATCTCG58450.039.81461740-41
ATCTCGT58700.039.768342-43
GTCGCGC853.5979065E-739.1813551
CTTGAAA59100.039.0256962-63
GATCGGA125000.038.708941
Produced by FastQC (version 0.11.2)