##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34302-1.341000000358ea.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8398502 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65863936211481 33.0 33.0 33.0 33.0 33.0 2 32.69923981681495 33.0 33.0 33.0 33.0 33.0 3 32.73481485150566 33.0 33.0 33.0 33.0 33.0 4 36.65286130788562 37.0 37.0 37.0 37.0 37.0 5 36.66681546304329 37.0 37.0 37.0 37.0 37.0 6 36.56032170975252 37.0 37.0 37.0 37.0 37.0 7 36.468229334231275 37.0 37.0 37.0 37.0 37.0 8 36.422029309512574 37.0 37.0 37.0 37.0 37.0 9 36.47241198489921 37.0 37.0 37.0 37.0 37.0 10-11 36.40468026321837 37.0 37.0 37.0 37.0 37.0 12-13 36.37758215691322 37.0 37.0 37.0 37.0 37.0 14-15 38.265055661116705 40.0 37.0 40.0 37.0 40.0 16-17 38.275529671839095 40.0 37.0 40.0 37.0 40.0 18-19 38.255622133566206 40.0 37.0 40.0 37.0 40.0 20-21 38.26184318346296 40.0 37.0 40.0 37.0 40.0 22-23 38.09838700996916 40.0 37.0 40.0 37.0 40.0 24-25 38.12654280489544 40.0 37.0 40.0 37.0 40.0 26-27 38.059619798864134 40.0 37.0 40.0 37.0 40.0 28-29 37.91544301590927 40.0 37.0 40.0 33.0 40.0 30-31 37.89903693539634 40.0 37.0 40.0 33.0 40.0 32-33 37.77310727555938 40.0 37.0 40.0 33.0 40.0 34-35 37.61682666742236 40.0 37.0 40.0 33.0 40.0 36-37 37.343214420857436 40.0 37.0 40.0 33.0 40.0 38-39 37.47529541577772 40.0 37.0 40.0 33.0 40.0 40-41 37.276750663392114 40.0 37.0 40.0 33.0 40.0 42-43 37.081944553921645 40.0 37.0 40.0 33.0 40.0 44-45 37.12333961461223 40.0 37.0 40.0 33.0 40.0 46-47 37.03747096803692 40.0 37.0 40.0 33.0 40.0 48-49 36.845436245654284 37.0 37.0 40.0 33.0 40.0 50-51 36.74784461562312 37.0 37.0 40.0 33.0 40.0 52-53 36.64701211001676 37.0 37.0 40.0 33.0 40.0 54-55 36.43102942643819 37.0 37.0 40.0 33.0 40.0 56-57 36.27679209935296 37.0 37.0 40.0 33.0 40.0 58-59 35.969255826812926 37.0 37.0 40.0 33.0 40.0 60-61 35.72413491120202 37.0 37.0 40.0 30.0 40.0 62-63 35.50227082162986 37.0 33.0 40.0 27.0 40.0 64-65 35.21992195751099 37.0 33.0 37.0 27.0 40.0 66-67 34.70557219608925 37.0 33.0 37.0 27.0 40.0 68-69 34.484180274053635 37.0 33.0 37.0 27.0 40.0 70-71 34.0509146750218 37.0 33.0 37.0 27.0 40.0 72-73 33.37219845872514 37.0 33.0 37.0 24.5 37.0 74-75 32.61341266573491 37.0 33.0 37.0 22.0 37.0 76-77 29.975607792913543 33.0 30.0 35.0 22.0 37.0 78-79 32.24006025122099 35.0 33.0 37.0 22.0 37.0 80-81 32.54917162608284 37.0 33.0 37.0 22.0 37.0 82-83 32.41168311920387 37.0 33.0 37.0 22.0 37.0 84-85 32.27980823246813 37.0 33.0 37.0 22.0 37.0 86-87 32.187608397307045 37.0 33.0 37.0 22.0 37.0 88-89 31.933498378639428 37.0 33.0 37.0 15.0 37.0 90-91 31.90884832795182 33.0 33.0 37.0 15.0 37.0 92-93 31.742506699409013 33.0 33.0 37.0 15.0 37.0 94-95 31.56117430227438 33.0 33.0 37.0 15.0 37.0 96-97 31.455676024129065 33.0 33.0 37.0 15.0 37.0 98-99 31.340508283501034 33.0 33.0 37.0 15.0 37.0 100-101 29.806003737333157 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1570390261705512 2306 2 0.1315600583446752 2306 3 0.11838280210398722 2306 4 0.16844801437176216 2306 5 0.1557505730409403 2306 6 0.1665373139439268 2306 7 0.1929683038925134 2306 8 0.24187913201756572 2306 9 0.21529700073827485 2306 10-11 0.21805236776959447 2306 12-13 0.2326709668721989 2306 14-15 0.4732449876450957 2306 16-17 0.3970954240288407 2306 18-19 0.43022441654965604 2306 20-21 0.4320572218985248 2306 22-23 0.44643978876808177 2306 24-25 0.451465402650328 2306 26-27 0.39821598782960166 2306 28-29 0.4160675887873495 2306 30-31 0.4810547469707771 2306 32-33 0.5321072332244228 2306 34-35 0.46404061882247305 2306 36-37 0.41835560779708203 2306 38-39 0.45538237181244057 2306 40-41 0.6039283190812199 2306 42-43 0.5566555898022898 2306 44-45 0.5230075626672956 2306 46-47 0.6732214270211756 2306 48-49 0.6241058384736036 2306 50-51 0.5692628828558668 2306 52-53 0.5348822333714338 2306 54-55 0.5571333674738881 2306 56-57 0.582160252088876 2306 58-59 0.6585591375153186 2306 60-61 0.595449268278962 2306 62-63 0.6010906522058264 2306 64-65 0.5389064739664491 2306 66-67 0.4858778771946035 2306 68-69 0.4411787991609515 2306 70-71 0.38346544894515233 2306 72-73 0.38915869837686046 2306 74-75 0.48886728719443084 2306 76-77 0.05312816934139519 2306 78-79 0.4312587123092584 2306 80-81 0.5749741486514779 2306 82-83 0.6193764415613074 2306 84-85 0.6583856164800395 2306 86-87 0.6983815917016258 2306 88-89 0.9351349082115661 2306 90-91 0.7187529385187048 2306 92-93 0.6799881197602318 2306 94-95 0.628724621099277 2306 96-97 0.6020794502838811 2306 98-99 0.5861147565567002 2306 100-101 0.4704653059513326 2307 1 0.14173181416273906 2307 2 0.11199230812385963 2307 3 0.0944237666105181 2307 4 0.17453102132169107 2307 5 0.15726542300142654 2307 6 0.15372373891254654 2307 7 0.19836924701463943 2307 8 0.20360946765979548 2307 9 0.18801335806708153 2307 10-11 0.20931682984713262 2307 12-13 0.20608718415638094 2307 14-15 0.3847998679970104 2307 16-17 0.40808707510271347 2307 18-19 0.3288139100064953 2307 20-21 0.3445609263985432 2307 22-23 0.3537114317646868 2307 24-25 0.3748054960908931 2307 26-27 0.4108886465855903 2307 28-29 0.4160688599393154 2307 30-31 0.3745298512778774 2307 32-33 0.3441872082953168 2307 34-35 0.42843147553531935 2307 36-37 0.3922683007216321 2307 38-39 0.40935196149910524 2307 40-41 0.4219836163709729 2307 42-43 0.4208311860088827 2307 44-45 0.48049424825838827 2307 46-47 0.5759372613617444 2307 48-49 0.4340168650995153 2307 50-51 0.5257602490289841 2307 52-53 0.4679221242993279 2307 54-55 0.3755411370452677 2307 56-57 0.35572214338866814 2307 58-59 0.517032345990053 2307 60-61 0.4201238170563286 2307 62-63 0.45772286944352203 2307 64-65 0.4924722005133333 2307 66-67 0.3362434968194492 2307 68-69 0.3718696012505802 2307 70-71 0.2995218416047223 2307 72-73 0.2999677757093835 2307 74-75 0.2536569587578086 2307 76-77 -0.09151821070834387 2307 78-79 0.2944847579951855 2307 80-81 0.4473032767719758 2307 82-83 0.5466830739238233 2307 84-85 0.48175469218548983 2307 86-87 0.4740287170096096 2307 88-89 0.6538266168141433 2307 90-91 0.5160476002615297 2307 92-93 0.4991796453169286 2307 94-95 0.5284143873816411 2307 96-97 0.5528065128913795 2307 98-99 0.39404097017996875 2307 100-101 0.43977915264605016 2201 1 0.14439013728568284 2201 2 0.15021360904571424 2201 3 0.11886959182284329 2201 4 0.17308114112621809 2201 5 0.1756671117367219 2201 6 0.1646201016880724 2201 7 0.17270118105157906 2201 8 0.23747868873719113 2201 9 0.19714522563793935 2201 10-11 0.2134990609512144 2201 12-13 0.23750157536487393 2201 14-15 0.3811828055890629 2201 16-17 0.36179063507908893 2201 18-19 0.3219272316274484 2201 20-21 0.37542549898928 2201 22-23 0.38430729174753964 2201 24-25 0.4036580257443845 2201 26-27 0.39513870236821447 2201 28-29 0.41862551035215034 2201 30-31 0.41289614021926724 2201 32-33 0.4757223624879643 2201 34-35 0.4413862148313612 2201 36-37 0.4842431368429061 2201 38-39 0.5493039849893151 2201 40-41 0.4824742590814708 2201 42-43 0.4650945044563386 2201 44-45 0.48274902200957115 2201 46-47 0.5655149523709255 2201 48-49 0.48177685994701136 2201 50-51 0.45953061116665594 2201 52-53 0.48345868271616155 2201 54-55 0.4336923957812573 2201 56-57 0.4764683853544014 2201 58-59 0.5648753372083348 2201 60-61 0.38547350366834365 2201 62-63 0.45988079972272544 2201 64-65 0.48503962111276877 2201 66-67 0.4339327876783443 2201 68-69 0.4292294316280305 2201 70-71 0.29104315313118434 2201 72-73 0.19329549825645387 2201 74-75 0.24071652238391295 2201 76-77 0.06368972100977643 2201 78-79 0.23185314893640196 2201 80-81 0.4087382129392978 2201 82-83 0.4956355259349934 2201 84-85 0.4912093795425818 2201 86-87 0.407318779383651 2201 88-89 0.6742029588495626 2201 90-91 0.3938969123611997 2201 92-93 0.5337851650572745 2201 94-95 0.5146903578416833 2201 96-97 0.43385460392310904 2201 98-99 0.4534039734654236 2201 100-101 0.42418966972144645 1101 1 0.14973841354162687 1101 2 0.10614374883358835 1101 3 0.09402767484406382 1101 4 0.1155898988653945 1101 5 0.11799884868982247 1101 6 0.1217131502926847 1101 7 0.09144032472467245 1101 8 0.13089614319957832 1101 9 0.1464692503788072 1101 10-11 0.15186971666587823 1101 12-13 0.17371980866550274 1101 14-15 0.250702786552516 1101 16-17 0.3038517850170237 1101 18-19 0.2958402484960416 1101 20-21 0.26731941717108754 1101 22-23 0.2980998320344028 1101 24-25 0.23051479982960643 1101 26-27 0.22682709283149904 1101 28-29 0.23160278377037002 1101 30-31 0.30479494792673734 1101 32-33 0.2968316155793218 1101 34-35 0.2552500224493528 1101 36-37 0.25820143005385887 1101 38-39 0.2711294903916368 1101 40-41 0.42078351299772976 1101 42-43 0.3552859031088147 1101 44-45 0.3250889946535054 1101 46-47 0.393970168939326 1101 48-49 0.39082795735281906 1101 50-51 0.38026907190273107 1101 52-53 0.2686875654036669 1101 54-55 0.35951962203052545 1101 56-57 0.4121671647624865 1101 58-59 0.400667137347412 1101 60-61 0.3650032882970393 1101 62-63 0.45558590025635937 1101 64-65 0.37897310347351976 1101 66-67 0.45099593500414414 1101 68-69 0.45708928358649104 1101 70-71 0.2589863917919928 1101 72-73 0.15029338405216208 1101 74-75 0.16698051299655248 1101 76-77 -0.17761282733782124 1101 78-79 0.2501357838758338 1101 80-81 0.3636856578561378 1101 82-83 0.44757684391029073 1101 84-85 0.4724221588600628 1101 86-87 0.39952038510387666 1101 88-89 0.6264812882124033 1101 90-91 0.40982329486459435 1101 92-93 0.5330946110287726 1101 94-95 0.48616928900867507 1101 96-97 0.4021938941622274 1101 98-99 0.2711504968630045 1101 100-101 0.26264073983903913 2304 1 0.12290539457169558 2304 2 0.1022724022698327 2304 3 0.10428830841370029 2304 4 0.14919629241078525 2304 5 0.15819275839399438 2304 6 0.13713069574750847 2304 7 0.15712703083416102 2304 8 0.12924623590674855 2304 9 0.09218757006329525 2304 10-11 0.12364223421101173 2304 12-13 0.1500251800510597 2304 14-15 0.24834756887135967 2304 16-17 0.2859657622400462 2304 18-19 0.2704098212191397 2304 20-21 0.27988830323902647 2304 22-23 0.2340469254882933 2304 24-25 0.23404418673155902 2304 26-27 0.22660116829640486 2304 28-29 0.2420880036944979 2304 30-31 0.316818411406949 2304 32-33 0.25588017670608565 2304 34-35 0.34186809353358427 2304 36-37 0.3134873504788658 2304 38-39 0.3180621603997551 2304 40-41 0.40271773787257814 2304 42-43 0.34250079679323875 2304 44-45 0.3397050294035395 2304 46-47 0.4069497376772233 2304 48-49 0.3649325370984897 2304 50-51 0.34143468430116286 2304 52-53 0.3184633834770807 2304 54-55 0.36306004148809023 2304 56-57 0.2838635190153269 2304 58-59 0.4968866245002914 2304 60-61 0.29738479458563916 2304 62-63 0.27483703377462376 2304 64-65 0.27066341232204394 2304 66-67 0.31227201569798524 2304 68-69 0.3541202414728133 2304 70-71 0.24584216736558773 2304 72-73 -0.016388052163343048 2304 74-75 0.09897050729134804 2304 76-77 0.10923139825032635 2304 78-79 0.263993248649669 2304 80-81 0.2811754741035202 2304 82-83 0.4114344296160013 2304 84-85 0.30034204613093607 2304 86-87 0.3132764990527974 2304 88-89 0.603113829886027 2304 90-91 0.4196341708907454 2304 92-93 0.36266751810143205 2304 94-95 0.3796092838605425 2304 96-97 0.30169552993437065 2304 98-99 0.40457950317053104 2304 100-101 0.4039620181278316 2202 1 0.09252746176359494 2202 2 0.08395163062608191 2202 3 0.056923594335124506 2202 4 0.08435201710371842 2202 5 0.0775830744558661 2202 6 0.07670678482890025 2202 7 0.10845920250203989 2202 8 0.08340368176129687 2202 9 0.030144410563849533 2202 10-11 0.07578301067478321 2202 12-13 0.04897210957206255 2202 14-15 0.015856577106731606 2202 16-17 0.041935275858506316 2202 18-19 0.03361980311910173 2202 20-21 0.15759159763725705 2202 22-23 0.15804030481806564 2202 24-25 0.12454153657375855 2202 26-27 0.10669217347466997 2202 28-29 0.1409288693749673 2202 30-31 0.09497208935540868 2202 32-33 0.10978186051415406 2202 34-35 0.11995455766479779 2202 36-37 0.1471249331393878 2202 38-39 0.17298738029470684 2202 40-41 0.1522764879009273 2202 42-43 0.21750899371122756 2202 44-45 0.18068870725987551 2202 46-47 0.25183777005316443 2202 48-49 0.19480184044984838 2202 50-51 0.146165737823857 2202 52-53 0.05874540697416819 2202 54-55 0.062175904891994094 2202 56-57 0.1615648654508135 2202 58-59 0.20702510790243878 2202 60-61 0.1556964566817598 2202 62-63 0.09150122513287329 2202 64-65 0.13430488253363393 2202 66-67 0.04721575653140775 2202 68-69 0.02286279620447118 2202 70-71 0.06782608112567345 2202 72-73 0.05610920439510636 2202 74-75 0.05518179767611997 2202 76-77 -0.23153298815542556 2202 78-79 0.19493125169511671 2202 80-81 0.24078722473909409 2202 82-83 0.30849359208450977 2202 84-85 0.3156658133772723 2202 86-87 0.30124685513783334 2202 88-89 0.45078144124786945 2202 90-91 0.14624055836617345 2202 92-93 0.2467489340598945 2202 94-95 0.33268485487487354 2202 96-97 0.2389922932468096 2202 98-99 0.26777698351895296 2202 100-101 0.25036299857570654 1102 1 0.08602395418648712 1102 2 0.08704882345760723 1102 3 0.05268841337402108 1102 4 0.06885033882013403 1102 5 0.08599359990686395 1102 6 0.06625377146769296 1102 7 0.07233569584388988 1102 8 0.10716131624207037 1102 9 0.08472210886056075 1102 10-11 0.09480934086268888 1102 12-13 0.10319964171968365 1102 14-15 0.17860636927621698 1102 16-17 0.18515802638179224 1102 18-19 0.1383003244013068 1102 20-21 0.12470389003469506 1102 22-23 0.09684456171671485 1102 24-25 0.032690802650236606 1102 26-27 0.11064358752116732 1102 28-29 0.12086698326401546 1102 30-31 0.15204462501414895 1102 32-33 0.2087095348638357 1102 34-35 0.1245489897633334 1102 36-37 0.08356943276303497 1102 38-39 0.17282789104262974 1102 40-41 0.16862257229335142 1102 42-43 0.1682641835751184 1102 44-45 0.11969723949928124 1102 46-47 0.20177677949805428 1102 48-49 0.11225326343198816 1102 50-51 0.06150018428812842 1102 52-53 0.07503802683242355 1102 54-55 0.07982211334304168 1102 56-57 0.04832782834777305 1102 58-59 0.0984430777837133 1102 60-61 0.11451896685689888 1102 62-63 0.11119002935243572 1102 64-65 0.0041094743074339135 1102 66-67 0.06562647297458568 1102 68-69 0.028123004458294076 1102 70-71 -0.16031554891438304 1102 72-73 -0.16536775286358107 1102 74-75 -0.16918258459636348 1102 76-77 -0.09184693647991438 1102 78-79 0.08960658399703192 1102 80-81 0.15185581699849138 1102 82-83 0.22615012838350168 1102 84-85 0.17799218203408884 1102 86-87 0.19985724850720743 1102 88-89 0.3484637055701256 1102 90-91 0.17571445092227123 1102 92-93 0.21078548913254025 1102 94-95 0.22361822566988465 1102 96-97 0.13395435441843517 1102 98-99 0.04231876035251503 1102 100-101 0.20265380867109428 2305 1 0.09545709589210816 2305 2 0.07982599903873933 2305 3 0.07706689997363725 2305 4 0.10307450267644214 2305 5 0.07574656733378049 2305 6 0.03235196097222115 2305 7 0.0663629123121936 2305 8 0.034809273616971836 2305 9 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-0.8676149882666095 1213 90-91 -0.8507591798983825 1213 92-93 -0.9389814955246791 1213 94-95 -0.9124990991016055 1213 96-97 -0.9515820030267221 1213 98-99 -0.9956478726811682 1213 100-101 -0.9222920672206492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 20.0 8 166.0 9 568.0 10 435.0 11 206.0 12 185.0 13 295.0 14 710.0 15 1614.0 16 3058.0 17 5146.0 18 7837.0 19 11492.0 20 15481.0 21 21062.0 22 27926.0 23 37193.0 24 48425.0 25 63284.0 26 85942.0 27 125658.0 28 207008.0 29 279161.0 30 204323.0 31 173275.0 32 226329.0 33 319241.0 34 491901.0 35 861740.0 36 1746225.0 37 2749445.0 38 682963.0 39 187.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.83530814872238 24.400775216911654 23.41927904827216 14.3446375860938 2 27.514763942426875 26.877947995964043 32.618578884663 12.988709176946077 3 34.948970540152374 18.331193660949683 30.809960657175907 15.90987514172204 4 26.500585053012056 18.317726957396825 33.60540928403207 21.576278705559044 5 35.63859364443802 17.333043440365913 31.480840273658327 15.547522641537743 6 29.010625942578805 18.47902161599771 36.95236364770766 15.557988793715833 7 30.345030578072134 24.570596042008443 28.528956711565947 16.555416668353477 8 26.405816180075924 31.37529764236527 25.926766463828905 16.2921197137299 9 34.328526682496474 23.35652238934991 24.59436218506586 17.720588743087756 10-11 26.115905409092537 29.391011602662175 27.52341336843818 16.969669619807107 12-13 23.156593878289247 27.766743402573457 27.175953521235098 21.900709197902195 14-15 22.368364024917778 28.380501665654183 27.650812013856758 21.60032229557128 16-17 24.396559417513103 26.286567441061866 28.517741496940456 20.79913164448457 18-19 20.41098519712206 26.553396069918183 31.746345955504925 21.289272777454837 20-21 23.567583838165426 26.83449381806422 31.302350109579063 18.295572234191287 22-23 20.681901489874953 32.914976698396906 28.097127132824944 18.3059946789032 24-25 20.821350045520024 26.14821667006807 31.519359047601586 21.511074236810327 26-27 20.57135903521842 26.249449008882774 28.334296997250224 24.844894958648577 28-29 23.076996350063382 29.70273746437162 28.00229731444965 19.21796887111535 30-31 19.49099375102846 27.14785922537138 31.079768749236468 22.28137827436369 32-33 19.436406635373785 30.06957074011532 28.513989756744714 21.98003286776618 34-35 22.807805487216648 30.286579678137837 28.574452920294597 18.331161914350915 36-37 19.426077898919583 30.80351880388959 31.720572031269683 18.049831265921146 38-39 19.99811750596808 27.38015532183206 30.752796384754156 21.868930787445706 40-41 23.093714007759512 26.958931969846674 30.62536038754108 19.32199363485274 42-43 20.16353685997369 30.184596061860187 31.056166722769763 18.595700355396357 44-45 19.565397290866862 27.500909101084183 31.260158177635137 21.673535430413818 46-47 22.954044044101256 27.3217257464997 28.172933301326204 21.55129690807285 48-49 20.30351118033786 30.478025584062006 30.562547262866406 18.655915972733723 50-51 23.112618630203603 27.59299747048452 30.02731824324477 19.267065656067103 52-53 19.660189102677638 27.414321686498443 27.4149051346113 25.510584076212613 54-55 22.94682573195684 27.3329639402312 30.391239929512842 19.328970398299123 56-57 19.916200992571344 27.08170388337808 30.056166711971205 22.945928412079372 58-59 19.857180236543037 26.896084535903952 30.632653238654505 22.614081988898505 60-61 22.555897455439226 27.106800579218625 30.875794282121387 19.46150768322076 62-63 19.690895343783673 27.20281123302514 30.685031166465244 22.421262256725942 64-65 22.993950115320253 27.28381583652063 30.41722203085706 19.305012017302055 66-67 20.2366696656791 33.16743178507306 27.035528321788416 19.56037022745942 68-69 20.221911045032932 33.057319060574116 27.06324132513528 19.657528569257675 70-71 19.812303608186728 34.074390915512744 27.056906925981135 19.056398550319393 72-73 19.78890967354303 34.155877203884835 26.88377263884466 19.171440483727473 74-75 20.607356578613402 32.95706222804567 26.835848417705648 19.599732775635275 76-77 20.853298872348937 31.798232905741493 27.41599971269278 19.93246850921679 78-79 20.704902107019098 31.52611586029741 27.91973731258677 19.849244720096724 80-81 20.11727763879004 31.848971294588157 27.73055330602297 20.30319776059883 82-83 19.390346876080372 31.57045455452009 28.10893915131653 20.930259418083008 84-85 19.491771155908328 31.083447105986895 28.347479248522937 21.07730248958184 86-87 19.389879123423302 31.080623209407925 28.91192859449896 20.617569072669813 88-89 19.566806265076597 31.406039631021933 28.560536543799024 20.466617560102446 90-91 20.10277812134681 30.632581019383508 28.16734602025085 21.097294839018836 92-93 19.689167652489676 31.383567800580526 27.856493394102362 21.07077115282744 94-95 19.456719993844757 31.885148022401843 27.681377793054722 20.976754190698674 96-97 19.907698634899543 32.20420902848037 27.12940265249702 20.758689684123066 98-99 20.184199444061726 31.197049225961994 27.114971234396062 21.50378009558022 100-101 20.235884634665705 31.366971066014603 26.79019131589228 21.606952983427412 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 115.0 1 85.5 2 129.5 3 379.5 4 444.5 5 271.5 6 226.0 7 231.5 8 229.5 9 226.0 10 226.5 11 231.0 12 217.0 13 225.0 14 253.0 15 289.0 16 350.0 17 444.5 18 578.0 19 809.5 20 1250.5 21 1928.0 22 2897.5 23 4339.0 24 6295.0 25 8908.5 26 12513.0 27 17307.0 28 22786.5 29 28855.5 30 35832.0 31 43688.0 32 55334.0 33 80648.5 34 124092.5 35 206351.5 36 320526.0 37 444137.5 38 582348.5 39 652284.0 40 655549.0 41 610606.5 42 546037.0 43 537599.5 44 514065.5 45 471921.5 46 460846.5 47 408459.0 48 311791.0 49 252310.0 50 208581.5 51 171193.5 52 148779.0 53 123734.5 54 91867.5 55 61341.0 56 42221.0 57 30902.5 58 23792.5 59 18269.0 60 13773.0 61 10260.5 62 7365.0 63 5203.0 64 3814.5 65 2833.0 66 2049.5 67 1537.0 68 1168.5 69 763.0 70 482.0 71 333.5 72 236.0 73 160.0 74 104.5 75 79.5 76 68.0 77 55.0 78 35.5 79 21.0 80 16.5 81 13.5 82 9.5 83 4.5 84 3.0 85 3.0 86 4.0 87 3.0 88 0.5 89 0.5 90 1.0 91 2.0 92 1.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16374348663606914 2 0.0 3 2.3813770598613893E-4 4 0.021753879441833796 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.941357399212384E-4 12-13 0.0 14-15 0.0 16-17 0.0011728282019817344 18-19 0.0 20-21 0.0 22-23 5.417632811184661E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.525508239445557E-4 38-39 8.215750856521793E-4 40-41 0.0050544728095558 42-43 0.0032505796867107967 44-45 0.001143060988733467 46-47 0.004453175101940798 48-49 0.007816870198995012 50-51 0.005370005269987433 52-53 0.0018396137787429234 54-55 0.01124009972254576 56-57 0.01934273516872414 58-59 0.023516098466131223 60-61 0.011692561363919423 62-63 6.191580355639613E-4 64-65 0.014699049901994426 66-67 0.035250333928598214 68-69 0.02040840140301211 70-71 0.037334038855976935 72-73 0.031166272270935935 74-75 0.030493533251525096 76-77 0.039382023127457734 78-79 0.01725903024134542 80-81 0.012841575795302543 82-83 8.394354136011399E-4 84-85 0.010799544966471402 86-87 0.02188485518012617 88-89 0.021396672882854587 90-91 0.025748639459751272 92-93 0.043519665768966896 94-95 0.02867177980073113 96-97 0.030082745708699003 98-99 0.03745310770897001 100-101 0.01754479548852879 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8398502.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.91242135944122 #Duplication Level Percentage of deduplicated Percentage of total 1 89.53396613549103 31.25847551704204 2 4.762720437216456 3.32556205442646 3 1.5283439626344153 1.6007456521695238 4 0.882814670377465 1.2328479101805707 5 0.5008015423879153 0.8742097232652482 6 0.3572727667943315 0.748395442474625 7 0.24672963645568521 0.6029750320861842 8 0.2020844291346403 0.5644205392104562 9 0.19833503210699008 0.6231920590131788 >10 1.3672378578694406 9.65917656611328 >50 0.19073277702455455 4.667365453327761 >100 0.1706407861424167 13.006562360668298 >500 0.031101387355051795 7.477833837642511 >1k 0.026363459153704104 16.578648211226273 >5k 6.158387288252697E-4 1.3303829839938803 >10k+ 2.3928112705829134E-4 6.449206657159672 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 462805 5.5105660509457515 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16677 0.19857112613654196 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 14155 0.16854196141168984 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13598 0.16190982629997588 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 11703 0.1393462786577892 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 10872 0.12945165697406516 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10591 0.1261058222049599 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9918 0.1180924883985263 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 9125 0.10865032835617591 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0019408223037870325 0.0 0.0 2 0.002036077386181488 0.0 0.0 3 0.002286121977466934 0.0 0.0 4 0.0024409114863579245 0.0 0.0 5 0.002750490504139905 0.0 0.0 6 0.0030957901778198064 0.0 0.0 7 0.003488717392696936 0.0 0.0 8 0.004143596084158818 0.0 0.0 9 0.004619871496131096 0.0 0.0 10-11 0.007537058394461298 0.0 1.1906885299306947E-5 12-13 0.010376850538346005 0.0 1.1906885299306947E-5 14-15 0.013496454486764425 0.0 1.1906885299306947E-5 16-17 0.01638387417184636 0.0 1.1906885299306947E-5 18-19 0.020104775827879784 0.0 1.1906885299306947E-5 20-21 0.023926886008957312 0.0 2.976721324826737E-5 22-23 0.02886228996552004 0.0 3.5720655897920846E-5 24-25 0.034482339826792927 0.0 4.762754119722779E-5 26-27 0.040489363460293276 0.0 5.358098384688127E-5 28-29 0.0469607556204666 0.0 5.953442649653474E-5 30-31 0.07185805278131743 0.0 7.144131179584169E-5 32-33 0.07919269412569052 0.0 7.144131179584169E-5 34-35 0.08612845481253681 0.0 7.144131179584169E-5 36-37 0.09361193222315123 0.0 7.144131179584169E-5 38-39 0.10135140766770073 0.0 7.739475444549517E-5 40-41 0.10984697032875625 0.0 8.334819709514864E-5 42-43 0.11863425167964478 0.0 8.334819709514864E-5 44-45 0.12827287532943377 0.0 8.334819709514864E-5 46-47 0.13813772979990957 0.0 8.334819709514864E-5 48-49 0.1488658334545851 0.0 8.334819709514864E-5 50-51 0.1604214656375625 0.0 8.334819709514864E-5 52-53 0.17222714241182535 0.0 8.334819709514864E-5 54-55 0.1852592283719168 0.0 8.93016397448021E-5 56-57 0.19919028417210594 0.0 9.525508239445558E-5 58-59 0.2146751885038546 0.0 9.525508239445558E-5 60-61 0.2312078987419423 0.0 9.525508239445558E-5 62-63 0.24885390275551522 0.0 9.525508239445558E-5 64-65 0.26833356710518136 0.0 9.525508239445558E-5 66-67 0.28856336522870385 0.0 9.525508239445558E-5 68-69 0.31187109320209727 0.0 9.525508239445558E-5 70-71 0.3361909064259317 0.0 1.0120852504410906E-4 72-73 0.3627611209713351 0.0 1.0716196769376254E-4 74-75 0.39279028569618724 0.0 1.1311541034341602E-4 76-77 0.42649867797852525 0.0 1.1906885299306948E-4 78-79 0.4643566197876716 0.0 1.1906885299306948E-4 80-81 0.5070308967003877 0.0 1.1906885299306948E-4 82-83 0.5535570510074297 0.0 1.1906885299306948E-4 84-85 0.6068760833777262 0.0 1.1906885299306948E-4 86-87 0.6657496777401494 0.0 1.1906885299306948E-4 88-89 0.7317554963968574 0.0 1.2502229564272295E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 61520 0.0 82.01471 3 GATCGGA 61855 0.0 81.72876 1 CGGAAGA 61635 0.0 81.6536 4 ATCGGAA 62705 0.0 80.51782 2 GAAGAGC 62610 0.0 80.27852 6 GGAAGAG 65810 0.0 76.540955 5 GAGCACA 92305 0.0 54.442207 9 AGAGCAC 92360 0.0 54.420074 8 AAGAGCA 93255 0.0 53.862133 7 TTGTGTT 95835 0.0 47.165573 1 GCCGTCT 54770 0.0 45.519436 50-51 ATGCCGT 54595 0.0 45.45873 48-49 TCGTATG 54840 0.0 45.35456 44-45 CGTCTTC 54990 0.0 45.26187 52-53 TGCCGTC 54740 0.0 45.236324 50-51 TCTCGTA 54325 0.0 45.228836 42-43 TATGCCG 54570 0.0 45.22716 48-49 CGTATGC 54665 0.0 45.06114 46-47 CTCGTAT 54415 0.0 45.02801 44-45 CGTATCT 54270 0.0 44.997803 38-39 >>END_MODULE