FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34302-1.341000000358ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34302-1.341000000358ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8398502
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4628055.5105660509457515TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC166770.19857112613654196No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA141550.16854196141168984No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG135980.16190982629997588No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA117030.1393462786577892No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC108720.12945165697406516No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC105910.1261058222049599No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA99180.1180924883985263No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG91250.10865032835617591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG615200.082.014713
GATCGGA618550.081.728761
CGGAAGA616350.081.65364
ATCGGAA627050.080.517822
GAAGAGC626100.080.278526
GGAAGAG658100.076.5409555
GAGCACA923050.054.4422079
AGAGCAC923600.054.4200748
AAGAGCA932550.053.8621337
TTGTGTT958350.047.1655731
GCCGTCT547700.045.51943650-51
ATGCCGT545950.045.4587348-49
TCGTATG548400.045.3545644-45
CGTCTTC549900.045.2618752-53
TGCCGTC547400.045.23632450-51
TCTCGTA543250.045.22883642-43
TATGCCG545700.045.2271648-49
CGTATGC546650.045.0611446-47
CTCGTAT544150.045.0280144-45
CGTATCT542700.044.99780338-39
Produced by FastQC (version 0.11.2)