##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34298-1.341000000357c1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5348509 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.657664033097824 33.0 33.0 33.0 33.0 33.0 2 32.701723040944685 33.0 33.0 33.0 33.0 33.0 3 32.72892127506937 33.0 33.0 33.0 33.0 33.0 4 36.65508742716896 37.0 37.0 37.0 37.0 37.0 5 36.65878097989552 37.0 37.0 37.0 37.0 37.0 6 36.55166832476116 37.0 37.0 37.0 37.0 37.0 7 36.49014931077053 37.0 37.0 37.0 37.0 37.0 8 36.4280379821741 37.0 37.0 37.0 37.0 37.0 9 36.444122090848126 37.0 37.0 37.0 37.0 37.0 10-11 36.39000467233018 37.0 37.0 37.0 37.0 37.0 12-13 36.36606744047734 37.0 37.0 37.0 37.0 37.0 14-15 38.22387117606047 40.0 37.0 40.0 37.0 40.0 16-17 38.20179268652254 40.0 37.0 40.0 37.0 40.0 18-19 38.201785488254764 40.0 37.0 40.0 37.0 40.0 20-21 38.160107798266765 40.0 37.0 40.0 37.0 40.0 22-23 38.05265074808699 40.0 37.0 40.0 35.0 40.0 24-25 38.01558396928938 40.0 37.0 40.0 35.0 40.0 26-27 37.89711011049995 40.0 37.0 40.0 33.0 40.0 28-29 37.78751910111771 40.0 37.0 40.0 33.0 40.0 30-31 37.72225427684613 40.0 37.0 40.0 33.0 40.0 32-33 37.59694393334666 40.0 37.0 40.0 33.0 40.0 34-35 37.516143564496204 40.0 37.0 40.0 33.0 40.0 36-37 37.437057598669085 40.0 37.0 40.0 33.0 40.0 38-39 37.311793716716195 40.0 37.0 40.0 33.0 40.0 40-41 37.206032653212326 40.0 37.0 40.0 33.0 40.0 42-43 37.0361161400308 40.0 37.0 40.0 33.0 40.0 44-45 36.96517038673768 38.5 37.0 40.0 33.0 40.0 46-47 36.80660750500747 37.0 37.0 40.0 33.0 40.0 48-49 36.67418854488233 37.0 37.0 40.0 33.0 40.0 50-51 36.51373429492219 37.0 37.0 40.0 33.0 40.0 52-53 36.36866508030556 37.0 37.0 40.0 33.0 40.0 54-55 36.215735170306345 37.0 37.0 40.0 33.0 40.0 56-57 36.00923687330432 37.0 37.0 40.0 33.0 40.0 58-59 35.66597541483056 37.0 37.0 40.0 27.0 40.0 60-61 35.43529626667918 37.0 33.0 40.0 27.0 40.0 62-63 35.172183687079894 37.0 33.0 38.5 27.0 40.0 64-65 34.87343369899911 37.0 33.0 37.0 27.0 40.0 66-67 34.50990958414766 37.0 33.0 37.0 27.0 40.0 68-69 34.19739791033352 37.0 33.0 37.0 27.0 40.0 70-71 33.82239798044651 37.0 33.0 37.0 27.0 38.5 72-73 33.39620443753577 37.0 33.0 37.0 27.0 37.0 74-75 32.90915412127006 37.0 33.0 37.0 24.5 37.0 76-77 30.201144094550465 33.0 30.0 35.0 22.0 37.0 78-79 32.60546621497692 35.0 33.0 37.0 27.0 37.0 80-81 32.999353464675856 37.0 33.0 37.0 27.0 37.0 82-83 32.906621359335844 37.0 33.0 37.0 27.0 37.0 84-85 32.775872023399415 37.0 33.0 37.0 27.0 37.0 86-87 32.692609473032576 37.0 33.0 37.0 27.0 37.0 88-89 32.426911032588706 37.0 33.0 37.0 22.0 37.0 90-91 32.39783928567756 35.0 33.0 37.0 22.0 37.0 92-93 32.21216211845208 33.0 33.0 37.0 22.0 37.0 94-95 32.03315353867779 33.0 33.0 37.0 22.0 37.0 96-97 31.918819899153206 33.0 33.0 37.0 22.0 37.0 98-99 31.79265959915184 33.0 33.0 37.0 22.0 37.0 100-101 30.193901889292885 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.10050052605733129 2306 2 0.08993180783207322 2306 3 0.11483699152163496 2306 4 0.1637316854104256 2306 5 0.1694155106953943 2306 6 0.12957171420482183 2306 7 0.16310476158995613 2306 8 0.2251967906834338 2306 9 0.20506607628443874 2306 10-11 0.18936201137905329 2306 12-13 0.18530194031531977 2306 14-15 0.4072645089633582 2306 16-17 0.3664375636894377 2306 18-19 0.42690569819503565 2306 20-21 0.41295248169401333 2306 22-23 0.41855413026101473 2306 24-25 0.4335855920785008 2306 26-27 0.40979629917402605 2306 28-29 0.4181274012083591 2306 30-31 0.4489298956989174 2306 32-33 0.42954146974966534 2306 34-35 0.487842048046609 2306 36-37 0.32125137825509853 2306 38-39 0.5052147807966065 2306 40-41 0.46051268042570825 2306 42-43 0.5820442727361694 2306 44-45 0.4932995623476728 2306 46-47 0.6271019044214228 2306 48-49 0.4595556229534381 2306 50-51 0.5728613313742628 2306 52-53 0.5090868880874098 2306 54-55 0.5289501452526366 2306 56-57 0.532176496846752 2306 58-59 0.4826004697930344 2306 60-61 0.538673131660012 2306 62-63 0.42236837736417954 2306 64-65 0.5395950429990464 2306 66-67 0.552272577481844 2306 68-69 0.46523110718985805 2306 70-71 0.30419684567012695 2306 72-73 0.4306223952230894 2306 74-75 0.39980213275989485 2306 76-77 -0.03212457893426546 2306 78-79 0.35563634674150535 2306 80-81 0.4167754631388547 2306 82-83 0.3926327781657548 2306 84-85 0.4364020529410624 2306 86-87 0.4250540685795272 2306 88-89 0.6633316353490599 2306 90-91 0.45636493096801445 2306 92-93 0.4794167849939228 2306 94-95 0.368160797447473 2306 96-97 0.35307132261591434 2306 98-99 0.4750727710426048 2306 100-101 0.279070308114175 2307 1 0.1723468063671234 2307 2 0.1449878650309273 2307 3 0.10611729457574626 2307 4 0.18471514322801852 2307 5 0.16141793767827295 2307 6 0.14940279287435487 2307 7 0.19279489702242358 2307 8 0.21893353846352426 2307 9 0.21982601328883078 2307 10-11 0.19529419660193525 2307 12-13 0.25097279277173357 2307 14-15 0.4599095768591681 2307 16-17 0.47476134390157654 2307 18-19 0.3390884025076204 2307 20-21 0.3821359496577017 2307 22-23 0.43493806045585615 2307 24-25 0.3731859693248367 2307 26-27 0.46007496336373066 2307 28-29 0.46917145615532263 2307 30-31 0.4858214888924124 2307 32-33 0.4620924348187998 2307 34-35 0.5128417730693542 2307 36-37 0.4135029065674303 2307 38-39 0.5912443595943131 2307 40-41 0.4826104134010265 2307 42-43 0.5007507842423422 2307 44-45 0.4726470592914964 2307 46-47 0.5903516719814661 2307 48-49 0.5395978246659681 2307 50-51 0.572446648846153 2307 52-53 0.46869323514596317 2307 54-55 0.4531233167212676 2307 56-57 0.5149428588910041 2307 58-59 0.5825915414500855 2307 60-61 0.5266331124811927 2307 62-63 0.5681833134100813 2307 64-65 0.4538181065643485 2307 66-67 0.49634285021886626 2307 68-69 0.4082724880111286 2307 70-71 0.45336103267800354 2307 72-73 0.44251462815957865 2307 74-75 0.3525037449571258 2307 76-77 0.09556321144732038 2307 78-79 0.417952532867659 2307 80-81 0.561699108129524 2307 82-83 0.5425023098783726 2307 84-85 0.4586821407272623 2307 86-87 0.38168238396339405 2307 88-89 0.624217692857691 2307 90-91 0.5965618012106191 2307 92-93 0.5081345391413663 2307 94-95 0.3549753296696423 2307 96-97 0.5011077100748018 2307 98-99 0.39205683066755626 2307 100-101 0.3639333099588775 2201 1 0.14822173242802705 2201 2 0.11649569713119234 2201 3 0.1257031757550351 2201 4 0.16027015918046317 2201 5 0.1463194644786867 2201 6 0.1736346461740652 2201 7 0.1550917253490809 2201 8 0.2257636192793413 2201 9 0.183183573660763 2201 10-11 0.1783932599768221 2201 12-13 0.18521655389603353 2201 14-15 0.3672376100630572 2201 16-17 0.3533045824008241 2201 18-19 0.3625240113166157 2201 20-21 0.3901887236982944 2201 22-23 0.38421746293652603 2201 24-25 0.4326374379716995 2201 26-27 0.41383078025766906 2201 28-29 0.36038094158633527 2201 30-31 0.36907074952826235 2201 32-33 0.4501007006968365 2201 34-35 0.42840446900426343 2201 36-37 0.44158052874300324 2201 38-39 0.5440223910354831 2201 40-41 0.3946310678247613 2201 42-43 0.42286013561603397 2201 44-45 0.5083095201031824 2201 46-47 0.49493377442321673 2201 48-49 0.37949014442137496 2201 50-51 0.43768382019669616 2201 52-53 0.5377266802498895 2201 54-55 0.5293860080715191 2201 56-57 0.5646543486551323 2201 58-59 0.6564721329157308 2201 60-61 0.5047031140556015 2201 62-63 0.5806665214729279 2201 64-65 0.6070548612827977 2201 66-67 0.4596183199450792 2201 68-69 0.5382688967794209 2201 70-71 0.4119495785800993 2201 72-73 0.54628442640918 2201 74-75 0.4266845330069913 2201 76-77 0.1703773466166183 2201 78-79 0.4310839066285368 2201 80-81 0.5456722804036431 2201 82-83 0.5784164457912695 2201 84-85 0.6404200775349622 2201 86-87 0.5751714803516137 2201 88-89 0.7899050761647288 2201 90-91 0.4729752033899004 2201 92-93 0.5888215926414091 2201 94-95 0.5847563287317925 2201 96-97 0.5501544234237308 2201 98-99 0.5610200560376484 2201 100-101 0.614502715179114 1101 1 0.1285025268340334 1101 2 0.12008707850439038 1101 3 0.12088750453018804 1101 4 0.14194156391524615 1101 5 0.11771485049685992 1101 6 0.1503927610716147 1101 7 0.153472928440209 1101 8 0.148275685997163 1101 9 0.16618067357654098 1101 10-11 0.1629631752384526 1101 12-13 0.1552415909800331 1101 14-15 0.25983862086076215 1101 16-17 0.2661183752173386 1101 18-19 0.24281123330582233 1101 20-21 0.2768918244834353 1101 22-23 0.2949188824193456 1101 24-25 0.28477300125838667 1101 26-27 0.25313448759618495 1101 28-29 0.30354674613558075 1101 30-31 0.3742395198145516 1101 32-33 0.3933659680388075 1101 34-35 0.29388636537441215 1101 36-37 0.31827639394806795 1101 38-39 0.35343001719819966 1101 40-41 0.4127921131592913 1101 42-43 0.45605734876940573 1101 44-45 0.4232064744369026 1101 46-47 0.45741879414635633 1101 48-49 0.4388548011822806 1101 50-51 0.40753722556420513 1101 52-53 0.31163838394449783 1101 54-55 0.3431383263906156 1101 56-57 0.38814964380685524 1101 58-59 0.5380448223655421 1101 60-61 0.42198122485955736 1101 62-63 0.49107429826829474 1101 64-65 0.40351341539525976 1101 66-67 0.41324699556246003 1101 68-69 0.3970723768411588 1101 70-71 0.23846069871704145 1101 72-73 0.15407993897254357 1101 74-75 0.15400090588097726 1101 76-77 -0.13947413686909016 1101 78-79 0.2138165953446176 1101 80-81 0.4125353940773877 1101 82-83 0.5643664472592391 1101 84-85 0.44592795078341396 1101 86-87 0.42362005995654073 1101 88-89 0.673605064514426 1101 90-91 0.4472987051765642 1101 92-93 0.44296438639364055 1101 94-95 0.5723511669349648 1101 96-97 0.44744833221411184 1101 98-99 0.4443003827103382 1101 100-101 0.40752056974626427 2304 1 0.13863001045645973 2304 2 0.06696699654778371 2304 3 0.06769441360069806 2304 4 0.085346245880217 2304 5 0.08799495157392556 2304 6 0.1340116194328118 2304 7 0.11013497255063243 2304 8 0.09866532654412197 2304 9 0.1152082124445073 2304 10-11 0.09402243453192938 2304 12-13 0.11866815411089249 2304 14-15 0.2764506062882006 2304 16-17 0.2583892677462032 2304 18-19 0.2393630168257843 2304 20-21 0.26047725029593494 2304 22-23 0.2729210277530285 2304 24-25 0.26445417358517176 2304 26-27 0.25314020543650173 2304 28-29 0.2419170827154602 2304 30-31 0.3175670445695502 2304 32-33 0.24886867629948028 2304 34-35 0.28350805915656707 2304 36-37 0.28683939394237257 2304 38-39 0.36759606749662765 2304 40-41 0.22176030129318036 2304 42-43 0.37594684063008543 2304 44-45 0.2942463935278923 2304 46-47 0.44065103681023743 2304 48-49 0.40492284958113345 2304 50-51 0.3296688732094495 2304 52-53 0.3480508019456323 2304 54-55 0.39407840885039036 2304 56-57 0.2888912768462646 2304 58-59 0.45040483786246455 2304 60-61 0.402728426851219 2304 62-63 0.4430574448554623 2304 64-65 0.31469504005926296 2304 66-67 0.3560962173688793 2304 68-69 0.3232847910662642 2304 70-71 0.2428922716177766 2304 72-73 0.09341824986152147 2304 74-75 0.16664977770175682 2304 76-77 0.08065809637269084 2304 78-79 0.23380385554792582 2304 80-81 0.29928269141122854 2304 82-83 0.4740681603107433 2304 84-85 0.43884785182306985 2304 86-87 0.4440917221381042 2304 88-89 0.6418252407414045 2304 90-91 0.4542939782940252 2304 92-93 0.4552857717128873 2304 94-95 0.42665599885983596 2304 96-97 0.4040618531619735 2304 98-99 0.4496573936727657 2304 100-101 0.4499295896012505 2202 1 0.08674758518900916 2202 2 0.08683571096329246 2202 3 0.04915875448649132 2202 4 0.06071655390142183 2202 5 0.036288737892974154 2202 6 0.053542275477340695 2202 7 0.06042204257133932 2202 8 0.10069598719275064 2202 9 0.04885550934203309 2202 10-11 0.08693611278682312 2202 12-13 0.06817834449809368 2202 14-15 0.060999315439971724 2202 16-17 0.08230182460896884 2202 18-19 0.07238431055199612 2202 20-21 0.11460938270057142 2202 22-23 0.14278927338712322 2202 24-25 0.11710357547868 2202 26-27 0.15937789469235497 2202 28-29 0.17828553017670146 2202 30-31 0.15556999889701473 2202 32-33 0.21018196660426725 2202 34-35 0.16399591997804208 2202 36-37 0.06255925385039518 2202 38-39 0.06505759937719091 2202 40-41 0.11177943008636504 2202 42-43 0.2316048565495663 2202 44-45 0.21359091823627097 2202 46-47 0.37752493109299934 2202 48-49 0.28754862572829865 2202 50-51 0.22925380074839552 2202 52-53 0.17758400615200287 2202 54-55 0.05819204447965376 2202 56-57 0.14844566160162742 2202 58-59 0.264241189283112 2202 60-61 0.11947575182539794 2202 62-63 0.1742758010633807 2202 64-65 0.20708712364700688 2202 66-67 0.06612653245863243 2202 68-69 0.07862709944307511 2202 70-71 4.7380651863448975E-4 2202 72-73 -0.00895258286002587 2202 74-75 0.05171271495552787 2202 76-77 -0.2470214540257487 2202 78-79 0.08204815612855754 2202 80-81 0.03431977453270463 2202 82-83 0.2426853748349984 2202 84-85 0.3594007024912429 2202 86-87 0.20477041634077153 2202 88-89 0.3796295154587028 2202 90-91 0.19897655300229644 2202 92-93 0.2809302247814287 2202 94-95 0.18136953323231353 2202 96-97 0.13340261422525046 2202 98-99 0.25946542684507534 2202 100-101 0.19399534004579877 1102 1 0.1297223349055372 1102 2 0.08199847179716357 1102 3 0.031014152629808223 1102 4 0.08773566862249282 1102 5 0.07232267181472452 1102 6 0.034529082608067085 1102 7 0.05045410386178162 1102 8 0.12642821627108702 1102 9 0.06233020555256985 1102 10-11 0.03849625109079824 1102 12-13 0.06644856557105783 1102 14-15 0.1808869473776724 1102 16-17 0.16177255557912673 1102 18-19 0.097544640506122 1102 20-21 0.0622130742534992 1102 22-23 0.17842116815878484 1102 24-25 0.13607552251542643 1102 26-27 0.10046638983913425 1102 28-29 0.15173519999852658 1102 30-31 0.1713262202805126 1102 32-33 0.20510318667243865 1102 34-35 0.11152965421199212 1102 36-37 0.12201588524802531 1102 38-39 0.23500304206914535 1102 40-41 0.18384293049364686 1102 42-43 0.20130260489531082 1102 44-45 0.0787044723610606 1102 46-47 0.22438506956932258 1102 48-49 0.08182109154595452 1102 50-51 0.13701527151602733 1102 52-53 0.11176478401158363 1102 54-55 0.20077551891578338 1102 56-57 0.18493106700076822 1102 58-59 0.23953904843246931 1102 60-61 0.1927608221814623 1102 62-63 0.13691048457035748 1102 64-65 0.15041184405666996 1102 66-67 0.21604906236597543 1102 68-69 0.15300684907891338 1102 70-71 0.09012301404244027 1102 72-73 0.03388744222120721 1102 74-75 -0.03941916861356276 1102 76-77 -0.029374990583654892 1102 78-79 0.11649588814048428 1102 80-81 0.17851344240042266 1102 82-83 0.26670308163564727 1102 84-85 0.206904009600116 1102 86-87 0.277175438508543 1102 88-89 0.5487506682174441 1102 90-91 0.20928458377348846 1102 92-93 0.24127108378836226 1102 94-95 0.3971802437683536 1102 96-97 0.28182110094425283 1102 98-99 0.17734581643762226 1102 100-101 0.12059168302499401 2305 1 0.05190516361694364 2305 2 0.06391898251548156 2305 3 0.026347361325527174 2305 4 0.01517656058808825 2305 5 0.02577248284053013 2305 6 6.879714526846215E-4 2305 7 -0.003512374527495865 2305 8 0.03747825648288483 2305 9 0.01648483540844836 2305 10-11 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#Quality Count 7 7.0 8 112.0 9 330.0 10 279.0 11 132.0 12 132.0 13 323.0 14 883.0 15 2335.0 16 4724.0 17 7225.0 18 9408.0 19 10813.0 20 12910.0 21 15949.0 22 19953.0 23 24439.0 24 30074.0 25 38404.0 26 48529.0 27 62954.0 28 89226.0 29 122444.0 30 119633.0 31 125168.0 32 162584.0 33 227436.0 34 344730.0 35 594687.0 36 1171246.0 37 1734320.0 38 367070.0 39 50.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.45488801854943 24.982250805713228 24.306180202967255 14.256680972770086 2 30.85370147082112 23.121770945884172 32.871777910441956 13.152749672852751 3 36.78580115931721 18.02374986748604 28.670393697813573 16.52005527538318 4 29.906807016861435 17.669152473654417 33.854469487447005 18.56957102203714 5 31.33888341592021 16.632616678779076 35.62263800995754 16.40586189534317 6 26.741153469125695 17.491192405210498 39.55954827784715 16.208105847816654 7 33.12257677793942 20.06108618308392 29.32290101783506 17.49343602114159 8 29.724545663099754 28.535915336404965 24.596892330180243 17.142646670315035 9 30.40340775345054 25.183803560955027 25.4586091189152 18.954179566679237 10-11 24.925219415121735 27.285394421836255 29.44615765169192 18.343228511350095 12-13 24.002633257231125 26.140518787572386 28.85008700555613 21.00676094964036 14-15 23.273588957221534 26.845182461130758 29.606035999939422 20.275192581708286 16-17 23.13416894544022 26.837743501417094 30.64526397298509 19.382823580157595 18-19 20.94974505979143 27.06063502931378 31.84865165226421 20.140968258630583 20-21 22.367654237844604 27.31743556942692 31.133760829419938 19.181149363308542 22-23 21.073557347134138 29.746900053847074 30.178676932511667 19.00086566650712 24-25 21.557708886719645 26.699038928419117 31.550549882219514 20.192702302641727 26-27 21.276929701342933 26.750305552444615 30.28089697521309 21.691867770999355 28-29 21.93153269443877 28.33944936803883 29.845055883798643 19.883962053723756 30-31 20.198030890478076 27.81749081846922 30.716953079820936 21.267525211231767 32-33 19.91294209283372 29.24233650910936 30.21122335215291 20.63349804590401 34-35 21.500580816074162 28.060147229816756 30.359320700404542 20.079951253704536 36-37 19.91445961722123 28.879970862684416 31.695618259284714 19.50995126080964 38-39 20.495593696445006 29.871417529560766 29.519784521715913 20.11320425227831 40-41 20.507483993588554 27.862341823186004 32.16359190557281 19.466582277652634 42-43 20.39760953754321 29.342426501693282 31.362054096249537 18.897909864513974 44-45 20.018263214654848 28.505366782488256 31.511122088318572 19.965247914538324 46-47 21.64295260100584 28.06030177062011 30.32955999302578 19.96718563534827 48-49 20.918076473526074 30.083480535886476 30.177383517390282 18.82105947319717 50-51 21.543793439138717 29.242950288745845 29.790029964200208 19.42322630791523 52-53 19.945084670403787 28.81416000682819 29.20089136679826 22.039863955969764 54-55 21.696491788543387 28.65847302070177 30.12990366636627 19.515131524388572 56-57 20.57389993707381 28.439589455702485 29.58264882791791 21.403861779305792 58-59 20.393317166958433 28.304776623324653 30.28114307129581 21.02076313842111 60-61 21.151648673022923 28.404158860653332 30.52373573437234 19.920456731951397 62-63 20.182554567341803 28.390349188732582 30.03375910515187 21.39333713877374 64-65 21.621932689928002 28.20843034075961 29.839501464496195 20.33013550481619 66-67 20.93798168994894 30.789731416487687 28.27030624574565 20.001980647817717 68-69 21.47414490901473 30.733083664633842 28.059322823909888 19.733448602441538 70-71 20.747630322224555 31.56779815557999 28.26328556040993 19.421285961785525 72-73 20.27592960352022 31.671290336245374 28.31838413852699 19.73439592170742 74-75 21.359480319440312 30.77187352778963 27.838122363346617 20.030523789423444 76-77 21.747669512131253 29.50015150041522 28.483611769898925 20.268567217554594 78-79 21.533745296875267 29.449964063597005 29.171037362919712 19.845253276608016 80-81 20.50191745944127 30.082838249484862 29.091885187482063 20.3233591035918 82-83 19.58851706846457 29.920014359273512 29.21497029051353 21.276498281748392 84-85 19.406812883050424 29.30737929724923 29.88707898169791 21.398728838002434 86-87 19.66994776523003 29.33033199710663 30.503706850960725 20.49601338670261 88-89 19.751612079195997 29.967150648852293 30.06166312790302 20.21957414404869 90-91 20.21020187601233 29.6081701184154 29.44033439600927 20.741293609562998 92-93 19.561720042661392 30.354586342743357 29.286499076181112 20.797194538414143 94-95 19.46530222787933 30.743317166187463 29.326668983205344 20.46471162272786 96-97 20.139790941978607 31.086605917784127 28.543957813979077 20.22964532625819 98-99 20.431142849501192 30.392376352418076 28.318143382734572 20.858337415346163 100-101 20.23597134302435 30.303490435940546 28.38257210222495 21.077966118810153 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5119.0 1 3080.0 2 1158.5 3 1827.0 4 1923.0 5 1304.0 6 1127.5 7 1199.5 8 1282.5 9 1265.0 10 1164.0 11 1084.0 12 1057.0 13 1035.5 14 1024.5 15 1026.0 16 1081.5 17 1148.5 18 1275.5 19 1425.0 20 1636.0 21 1951.5 22 2356.0 23 3002.0 24 3930.0 25 5020.0 26 6541.5 27 8446.0 28 10704.5 29 13298.0 30 16633.0 31 21924.5 32 33530.0 33 55048.0 34 84691.0 35 126620.0 36 181880.0 37 272188.0 38 382057.0 39 443324.0 40 440796.0 41 386112.0 42 337351.5 43 329838.5 44 321557.0 45 309177.5 46 304902.5 47 271474.0 48 212100.0 49 169183.5 50 140477.0 51 116416.5 52 93344.0 53 66933.5 54 44201.5 55 30561.0 56 21126.5 57 14654.0 58 10230.5 59 7024.5 60 5030.5 61 3850.0 62 2790.5 63 1818.0 64 1295.5 65 943.5 66 658.5 67 471.5 68 355.5 69 263.5 70 182.5 71 143.5 72 117.0 73 82.5 74 62.0 75 42.5 76 27.0 77 19.5 78 14.5 79 10.0 80 10.5 81 11.5 82 5.0 83 2.0 84 2.5 85 3.5 86 3.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1650927389296718 2 0.0 3 2.9914879081254235E-4 4 0.022772701700604785 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.32858783634841E-4 12-13 0.0 14-15 0.0 16-17 0.0010189755687052223 18-19 0.0 20-21 0.0 22-23 5.42207183347733E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.665687764571397E-4 38-39 6.543879799024363E-4 40-41 0.004889213049842489 42-43 0.003206501101521938 44-45 9.909303695665465E-4 46-47 0.004515277061326811 48-49 0.008151804549641779 50-51 0.005487510631467574 52-53 0.0017481507463107944 54-55 0.010928279264370688 56-57 0.018210682640713516 58-59 0.02236137211323754 60-61 0.011255473254321906 62-63 4.8611678507038124E-4 64-65 0.014312399960437571 66-67 0.033158773781627744 68-69 0.019117477412864032 70-71 0.03544913171128627 72-73 0.02926983950106469 74-75 0.028643496720300927 76-77 0.03718793405788417 78-79 0.01621947350186753 80-81 0.012667081610968588 82-83 9.161431718634109E-4 84-85 0.010610433674132362 86-87 0.020809537760897476 88-89 0.019949484987311417 90-91 0.024604988044331606 92-93 0.0415629851235176 94-95 0.027129055966812435 96-97 0.02850327072460755 98-99 0.0351873765193253 100-101 0.017023435877176238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5348509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.616598366830424 #Duplication Level Percentage of deduplicated Percentage of total 1 85.38890104674893 27.85095490426836 2 6.615108866277955 4.3152469808849405 3 2.124194455514409 2.0785199222568456 4 1.0890149844344252 1.4207985745103096 5 0.7536768890479563 1.2291188194219702 6 0.5818894722691709 1.1387553126534273 7 0.3610476114964147 0.824330145483739 8 0.35915257291640884 0.9371468178582629 9 0.27091730925668633 0.7952760959982955 >10 1.8932946723857373 12.723153457387248 >50 0.2408667982614113 5.503419845690061 >100 0.25911053332964645 17.859686993621317 >500 0.043536055728087504 9.773542384722964 >1k 0.018826626599802533 10.02664783259577 >5k 2.3105424900473517E-4 0.5432646873025119 >10k+ 2.3105148397308128E-4 2.9801372253442167 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 116628 2.180570323430324 TruSeq Adapter, Index 2 (97% over 37bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16170 0.3023272467149256 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 13173 0.24629293883585124 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 12208 0.2282505273899698 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 9107 0.17027175237061395 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 8174 0.15282763850635758 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6050 0.11311563652599257 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 5504 0.10290718403951457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013461695586564405 0.0 0.0 2 0.00143965355578536 0.0 0.0 3 0.0015331375529142794 0.0 0.0 4 0.0017388023465979023 0.0 0.0 5 0.0021127383351135804 0.0 0.0 6 0.002393190326500339 0.0 0.0 7 0.002729732716164449 0.0 0.0 8 0.00321584950123483 0.0 0.0 9 0.0037019662863052114 0.0 0.0 10-11 0.006646712195866175 0.0 0.0 12-13 0.009956045694229924 0.0 0.0 14-15 0.01415347716531841 0.0 0.0 16-17 0.018967903017457762 0.0 0.0 18-19 0.024969575633134393 0.0 0.0 20-21 0.030419692665750397 0.0 0.0 22-23 0.036430713681139926 0.0 0.0 24-25 0.04336722626810575 0.0 0.0 26-27 0.05138815322176704 0.0 0.0 28-29 0.060241087749875716 0.0 0.0 30-31 0.08911829446299893 0.0 0.0 32-33 0.0996071989408637 0.0 0.0 34-35 0.1094791090376776 0.0 0.0 36-37 0.12011758791094865 0.0 9.348399712891949E-6 38-39 0.1327005339245012 0.0 1.8696799425783897E-5 40-41 0.14518999594092485 0.0 1.8696799425783897E-5 42-43 0.15819361994155756 0.0 1.8696799425783897E-5 44-45 0.17219752271146968 0.0 1.8696799425783897E-5 46-47 0.187482156242048 0.0 1.8696799425783897E-5 48-49 0.20371097814362843 0.0 1.8696799425783897E-5 50-51 0.22041656843056634 0.0 1.8696799425783897E-5 52-53 0.23740261070889102 0.0 1.8696799425783897E-5 54-55 0.25580026134386236 0.0 1.8696799425783897E-5 56-57 0.2748055579601717 0.0 1.8696799425783897E-5 58-59 0.2953907341279598 0.0 1.8696799425783897E-5 60-61 0.31829431342454506 0.0 1.8696799425783897E-5 62-63 0.34163726750763623 0.0 3.7393598851567794E-5 64-65 0.36674706913646404 0.0 3.7393598851567794E-5 66-67 0.39306281432825485 0.0 3.7393598851567794E-5 68-69 0.4204162318881767 0.0 3.7393598851567794E-5 70-71 0.4496860713892414 0.0 3.7393598851567794E-5 72-73 0.4807321068357555 0.0 3.7393598851567794E-5 74-75 0.5128906018481039 0.0 3.7393598851567794E-5 76-77 0.5466663700107824 0.0 4.674199856445974E-5 78-79 0.5847330536416784 0.0 5.609039827735169E-5 80-81 0.6250900952022329 0.0 5.609039827735169E-5 82-83 0.6687003798628739 0.0 5.609039827735169E-5 84-85 0.716106114806949 0.0 5.609039827735169E-5 86-87 0.7673727388324485 0.0 5.609039827735169E-5 88-89 0.8228835363276008 0.0 5.609039827735169E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 18695 0.0 66.819374 4 TCGGAAG 18795 0.0 66.716576 3 GAAGAGC 19070 0.0 65.51868 6 GATCGGA 19350 0.0 64.915886 1 ATCGGAA 19615 0.0 63.927498 2 GGAAGAG 20380 0.0 61.423733 5 TCGCCGG 100 0.0 52.247414 2 CGATCGC 40 5.345205E-4 47.580395 1 CACGCCG 105 2.0008883E-11 45.314663 1 CGAGTGG 170 0.0 44.78155 1 GCCGTCT 14510 0.0 42.99988 50-51 CGTCTTC 14465 0.0 42.98789 52-53 ATGCCGT 14570 0.0 42.587227 48-49 TGCCGTC 14555 0.0 42.57322 50-51 TATGCCG 14505 0.0 42.46701 48-49 CTGCTTG 14675 0.0 42.355556 58-59 TCGTATG 14635 0.0 42.3492 44-45 CGACGGG 225 0.0 42.293686 1 CGTATGC 14570 0.0 42.179573 46-47 TCTCGTA 14630 0.0 41.941658 42-43 >>END_MODULE