FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34298-1.341000000357c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34298-1.341000000357c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5348509
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1166282.180570323430324TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161700.3023272467149256No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC131730.24629293883585124No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG122080.2282505273899698No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA91070.17027175237061395No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA81740.15282763850635758No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA60500.11311563652599257No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT55040.10290718403951457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA186950.066.8193744
TCGGAAG187950.066.7165763
GAAGAGC190700.065.518686
GATCGGA193500.064.9158861
ATCGGAA196150.063.9274982
GGAAGAG203800.061.4237335
TCGCCGG1000.052.2474142
CGATCGC405.345205E-447.5803951
CACGCCG1052.0008883E-1145.3146631
CGAGTGG1700.044.781551
GCCGTCT145100.042.9998850-51
CGTCTTC144650.042.9878952-53
ATGCCGT145700.042.58722748-49
TGCCGTC145550.042.5732250-51
TATGCCG145050.042.4670148-49
CTGCTTG146750.042.35555658-59
TCGTATG146350.042.349244-45
CGACGGG2250.042.2936861
CGTATGC145700.042.17957346-47
TCTCGTA146300.041.94165842-43