FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34274-2.3410000003583d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34274-2.3410000003583d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4635548
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT294340.6349626840235502TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG255.2382413E-576.113191
CGCGGCT352.7892969E-454.2838674
CGGGTCG451.5033887E-552.8563771
CGCGAGG752.3574103E-950.7421261
CGACGGG652.5646877E-643.9114531
ACGCGGC459.629718E-442.220783
CGAGCGG802.2382847E-741.62441
CGAGGCG1051.0131771E-940.774921
CGACGAC608.222235E-539.6422841
GCGCGTT756.9284433E-637.9987074
GCGCACC1551.8189894E-1236.772942
CGAGCGA651.317223E-436.5928761
CGAACGG550.002561665634.5969051
CGCTCGG550.002561665634.5969051
CGAGGTG2850.033.3829771
GTGTTTG587450.032.464231
CGGTGCG600.00392349131.7138291
CGATCGG1051.8813425E-631.7138271
GTATCGC902.3900966E-531.7138251
AACGCGT1201.5097976E-731.6611468
Produced by FastQC (version 0.11.2)