##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34274-2.3410000003583d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4635548 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.678340079748935 33.0 33.0 33.0 33.0 33.0 2 32.72543958125339 33.0 33.0 33.0 33.0 33.0 3 32.75133403860773 33.0 33.0 33.0 33.0 33.0 4 36.68253375868398 37.0 37.0 37.0 37.0 37.0 5 36.686846085942804 37.0 37.0 37.0 37.0 37.0 6 36.582707157816074 37.0 37.0 37.0 37.0 37.0 7 36.53505497084703 37.0 37.0 37.0 37.0 37.0 8 36.48866153473117 37.0 37.0 37.0 37.0 37.0 9 36.49567041480317 37.0 37.0 37.0 37.0 37.0 10-11 36.45917969137629 37.0 37.0 37.0 37.0 37.0 12-13 36.43607562687303 37.0 37.0 37.0 37.0 37.0 14-15 38.38220162966709 40.0 37.0 40.0 37.0 40.0 16-17 38.36276282760959 40.0 37.0 40.0 37.0 40.0 18-19 38.36347029520566 40.0 37.0 40.0 37.0 40.0 20-21 38.30628859845697 40.0 37.0 40.0 37.0 40.0 22-23 38.21302033761704 40.0 37.0 40.0 37.0 40.0 24-25 38.17994905888149 40.0 37.0 40.0 37.0 40.0 26-27 38.10002787156988 40.0 37.0 40.0 37.0 40.0 28-29 37.99084520319927 40.0 37.0 40.0 35.0 40.0 30-31 37.939647912177804 40.0 37.0 40.0 33.0 40.0 32-33 37.82858499146164 40.0 37.0 40.0 33.0 40.0 34-35 37.743742271679636 40.0 37.0 40.0 33.0 40.0 36-37 37.65387436393712 40.0 37.0 40.0 33.0 40.0 38-39 37.58168484071355 40.0 37.0 40.0 33.0 40.0 40-41 37.51002308680657 40.0 37.0 40.0 33.0 40.0 42-43 37.35329080833593 40.0 37.0 40.0 33.0 40.0 44-45 37.27766900482963 40.0 37.0 40.0 33.0 40.0 46-47 37.12650014626102 40.0 37.0 40.0 33.0 40.0 48-49 37.0407944217167 40.0 37.0 40.0 33.0 40.0 50-51 36.9091584209677 37.0 37.0 40.0 33.0 40.0 52-53 36.76426940245253 37.0 37.0 40.0 33.0 40.0 54-55 36.62155811998926 37.0 37.0 40.0 33.0 40.0 56-57 36.42657200400039 37.0 37.0 40.0 33.0 40.0 58-59 36.18120554463032 37.0 37.0 40.0 33.0 40.0 60-61 35.941452121734045 37.0 37.0 40.0 33.0 40.0 62-63 35.68799395454432 37.0 37.0 40.0 27.0 40.0 64-65 35.428427663784305 37.0 33.0 40.0 27.0 40.0 66-67 35.15440838925625 37.0 33.0 37.0 27.0 40.0 68-69 34.863440309538376 37.0 33.0 37.0 27.0 40.0 70-71 34.54888397229411 37.0 33.0 37.0 27.0 40.0 72-73 34.25662154722592 37.0 33.0 37.0 27.0 40.0 74-75 33.877671097354614 37.0 33.0 37.0 27.0 37.0 76-77 31.113430709810363 33.0 30.0 35.0 24.5 37.0 78-79 33.530379148268985 35.0 33.0 37.0 27.0 37.0 80-81 33.89079511203422 37.0 33.0 37.0 27.0 37.0 82-83 33.76725081910489 37.0 33.0 37.0 27.0 37.0 84-85 33.62270577286655 37.0 33.0 37.0 27.0 37.0 86-87 33.519376781342785 37.0 33.0 37.0 27.0 37.0 88-89 33.25638338768145 37.0 33.0 37.0 27.0 37.0 90-91 33.22888502071384 37.0 33.0 37.0 27.0 37.0 92-93 33.07630780654197 37.0 33.0 37.0 27.0 37.0 94-95 32.896406530576314 35.0 33.0 37.0 27.0 37.0 96-97 32.79466775017754 33.0 33.0 37.0 27.0 37.0 98-99 32.66833781033009 33.0 33.0 37.0 27.0 37.0 100-101 31.060676105608227 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.10510229784831182 2306 2 0.08385082884910844 2306 3 0.10753490678332867 2306 4 0.15235260007958829 2306 5 0.15344436374492432 2306 6 0.12552492556211092 2306 7 0.20117773410991902 2306 8 0.16247455648577613 2306 9 0.1554087260300463 2306 10-11 0.19313496215241344 2306 12-13 0.17180071394589191 2306 14-15 0.39496620575074104 2306 16-17 0.45576793956357875 2306 18-19 0.4651830533997767 2306 20-21 0.3712441328032625 2306 22-23 0.34073542901155207 2306 24-25 0.3363091024920877 2306 26-27 0.4220088280444685 2306 28-29 0.37992718707256046 2306 30-31 0.39057522959426905 2306 32-33 0.4084851296291063 2306 34-35 0.41621388941977955 2306 36-37 0.3708558974599896 2306 38-39 0.4394728106169197 2306 40-41 0.5143044737727536 2306 42-43 0.5241463471832333 2306 44-45 0.4920474239450101 2306 46-47 0.6257761347500477 2306 48-49 0.5068035972790454 2306 50-51 0.5636902918697686 2306 52-53 0.46446641873757954 2306 54-55 0.5503949978623552 2306 56-57 0.4720650418498522 2306 58-59 0.5764905210920404 2306 60-61 0.587248945782818 2306 62-63 0.5392001989803319 2306 64-65 0.4976448985588391 2306 66-67 0.6388367018216456 2306 68-69 0.44126321161563453 2306 70-71 0.4085688380283372 2306 72-73 0.47039063047615315 2306 74-75 0.5207392650524127 2306 76-77 -0.03410663981560802 2306 78-79 0.24815010525230718 2306 80-81 0.43885603876556445 2306 82-83 0.6254593011604328 2306 84-85 0.5784554283754062 2306 86-87 0.44919272457944004 2306 88-89 0.7865551644046462 2306 90-91 0.4665103310072354 2306 92-93 0.5675012019010381 2306 94-95 0.45636731124099583 2306 96-97 0.5075149719000365 2306 98-99 0.3735543299164732 2306 100-101 0.37769135000424825 2307 1 0.13324087161582554 2307 2 0.12043268127091267 2307 3 0.11820570123050089 2307 4 0.1447199203449614 2307 5 0.130110257456586 2307 6 0.17616773301499933 2307 7 0.19152523259663212 2307 8 0.2267749769933758 2307 9 0.16072635532684387 2307 10-11 0.1747668910164606 2307 12-13 0.2204736907361493 2307 14-15 0.37031636942646884 2307 16-17 0.41100832355156314 2307 18-19 0.32164330092606974 2307 20-21 0.342810782103534 2307 22-23 0.3488077394543794 2307 24-25 0.3434050804156854 2307 26-27 0.34875261955943415 2307 28-29 0.3741204880622462 2307 30-31 0.3459893778831784 2307 32-33 0.3624881833033058 2307 34-35 0.4085664532713835 2307 36-37 0.31403336491808176 2307 38-39 0.4125291048296873 2307 40-41 0.3751741786545111 2307 42-43 0.4113669415153609 2307 44-45 0.4081942532735354 2307 46-47 0.4773692251345878 2307 48-49 0.3610072177199868 2307 50-51 0.42358978764210775 2307 52-53 0.35729806411786313 2307 54-55 0.3237382390735064 2307 56-57 0.3315586860516362 2307 58-59 0.5112297290841994 2307 60-61 0.37853166963611073 2307 62-63 0.3866096452935821 2307 64-65 0.36179198286212255 2307 66-67 0.41946414051978564 2307 68-69 0.20745717112283302 2307 70-71 0.2524456290002277 2307 72-73 0.26434582117017413 2307 74-75 0.28248642444276584 2307 76-77 -0.064626467115378 2307 78-79 0.24365517111912283 2307 80-81 0.3594193523132603 2307 82-83 0.47369149282918954 2307 84-85 0.4067774534773747 2307 86-87 0.45950484782661505 2307 88-89 0.645887843235819 2307 90-91 0.42748137330193003 2307 92-93 0.3775542848370961 2307 94-95 0.42186541047436776 2307 96-97 0.45263687292766264 2307 98-99 0.47717421875149313 2307 100-101 0.33765187910701044 2201 1 0.13413187835871554 2201 2 0.0906209938314575 2201 3 0.09774702694926418 2201 4 0.10023939735829401 2201 5 0.1102746058574624 2201 6 0.17254156481195082 2201 7 0.1956862321913846 2201 8 0.16806136489978485 2201 9 0.1330754965937615 2201 10-11 0.11992843832554456 2201 12-13 0.16406318803443298 2201 14-15 0.37115300259181794 2201 16-17 0.3044783600159633 2201 18-19 0.3203658060361434 2201 20-21 0.34355462969534756 2201 22-23 0.39861360494249 2201 24-25 0.3750866410851401 2201 26-27 0.3223298553317875 2201 28-29 0.3448794390045862 2201 30-31 0.3434667849038888 2201 32-33 0.3973593605518815 2201 34-35 0.4699967549651731 2201 36-37 0.4922133050844124 2201 38-39 0.4715719896681634 2201 40-41 0.3738100081139706 2201 42-43 0.3874376889285003 2201 44-45 0.3052576822304758 2201 46-47 0.4454909154746005 2201 48-49 0.31868875489702475 2201 50-51 0.2775166871981938 2201 52-53 0.2740303769330339 2201 54-55 0.3367620844449064 2201 56-57 0.36622411519841336 2201 58-59 0.3627055549136955 2201 60-61 0.33926319507641267 2201 62-63 0.3340829695596028 2201 64-65 0.32477972758821494 2201 66-67 0.21909371815455359 2201 68-69 0.2521059301264401 2201 70-71 0.06853330232073773 2201 72-73 0.2259275731702033 2201 74-75 0.20777656052637639 2201 76-77 -0.0016869197346771614 2201 78-79 0.2047360515315404 2201 80-81 0.3204010056830242 2201 82-83 0.4749983532400819 2201 84-85 0.3417988969386059 2201 86-87 0.29954270850863196 2201 88-89 0.5539043746979431 2201 90-91 0.3242058199465774 2201 92-93 0.3948782844816847 2201 94-95 0.43120809833649787 2201 96-97 0.40634800221381795 2201 98-99 0.48316701112646143 2201 100-101 0.39337627062026215 1101 1 0.14718705300437307 1101 2 0.11584846470859134 1101 3 0.09997854960769104 1101 4 0.1411880542828996 1101 5 0.11313073787015782 1101 6 0.11249125362612489 1101 7 0.15159698281264866 1101 8 0.14813548735712345 1101 9 0.13996806802290962 1101 10-11 0.09484591004520837 1101 12-13 0.1740397310670474 1101 14-15 0.24263426676927935 1101 16-17 0.2997858646350551 1101 18-19 0.22491559653052207 1101 20-21 0.2726865998449668 1101 22-23 0.2201176446028441 1101 24-25 0.2775027239834884 1101 26-27 0.28203886484493523 1101 28-29 0.23533691004954704 1101 30-31 0.29565013745835955 1101 32-33 0.31031599118406916 1101 34-35 0.2995681215636452 1101 36-37 0.2755818880052203 1101 38-39 0.28720954129442333 1101 40-41 0.3927426050598015 1101 42-43 0.4377986298992269 1101 44-45 0.43474414298146513 1101 46-47 0.47464840738790315 1101 48-49 0.39060838142995635 1101 50-51 0.32091614543497116 1101 52-53 0.2673989140824986 1101 54-55 0.3531778930542231 1101 56-57 0.41704501581915565 1101 58-59 0.412536523026823 1101 60-61 0.3668491617172336 1101 62-63 0.5231137138339008 1101 64-65 0.39052234140672937 1101 66-67 0.30775400271330966 1101 68-69 0.3196277778919594 1101 70-71 0.30141291164365214 1101 72-73 0.31882420773872866 1101 74-75 0.23781892275504646 1101 76-77 -0.07250142253834113 1101 78-79 0.15217740784164135 1101 80-81 0.25998999085937413 1101 82-83 0.4208735242681101 1101 84-85 0.425779781807222 1101 86-87 0.3598526449424 1101 88-89 0.6480075928134923 1101 90-91 0.4665291938305032 1101 92-93 0.5187904309594842 1101 94-95 0.5581542493646481 1101 96-97 0.5433306228336079 1101 98-99 0.33520935402945184 1101 100-101 0.2158220981128025 2304 1 0.10413661962687115 2304 2 0.09585480379172395 2304 3 0.10873216352015191 2304 4 0.13102895088795918 2304 5 0.10502862461372331 2304 6 0.11089670120816919 2304 7 0.1501725870366215 2304 8 0.1084016850503815 2304 9 0.10191685284461727 2304 10-11 0.13577920353176864 2304 12-13 0.1900746039079806 2304 14-15 0.2554153591909838 2304 16-17 0.20408103874158456 2304 18-19 0.19572909711325792 2304 20-21 0.21195484014790367 2304 22-23 0.2444845721082345 2304 24-25 0.2248987725827405 2304 26-27 0.16449004317421156 2304 28-29 0.1911712243445578 2304 30-31 0.27403834600455923 2304 32-33 0.19979322997756555 2304 34-35 0.3372192751906411 2304 36-37 0.33683663666028707 2304 38-39 0.24451033429299684 2304 40-41 0.30807551423031754 2304 42-43 0.34821849415057926 2304 44-45 0.27148304239575083 2304 46-47 0.2734015336785873 2304 48-49 0.33786832661484567 2304 50-51 0.2906438115910035 2304 52-53 0.2818854782775375 2304 54-55 0.3263794264057296 2304 56-57 0.13375022991212404 2304 58-59 0.24372722825493298 2304 60-61 0.25670759226711937 2304 62-63 0.2992423940096458 2304 64-65 0.25199260720877703 2304 66-67 0.2293839850873951 2304 68-69 0.21334707067686765 2304 70-71 0.20219837689395348 2304 72-73 0.17746152239683255 2304 74-75 0.05568123851175244 2304 76-77 -0.07693706529611077 2304 78-79 0.25560877602513443 2304 80-81 0.34060288617793333 2304 82-83 0.3732463444053167 2304 84-85 0.33848238026193656 2304 86-87 0.3056778652372145 2304 88-89 0.565679261593921 2304 90-91 0.3396683291135716 2304 92-93 0.3702909482911565 2304 94-95 0.3753177622328252 2304 96-97 0.2830246854252678 2304 98-99 0.32779998488793893 2304 100-101 0.37528456663820364 2202 1 0.13981082466089134 2202 2 0.11241506129205447 2202 3 0.06016039427106534 2202 4 0.04268440157038356 2202 5 0.05141965303928231 2202 6 0.055804834970842876 2202 7 0.10813858916989716 2202 8 0.10218699119590013 2202 9 0.07495800851607015 2202 10-11 0.15159950822662438 2202 12-13 0.11963957663775915 2202 14-15 0.059196249986946725 2202 16-17 0.09908103711709515 2202 18-19 0.04182901787088866 2202 20-21 0.09715939039467969 2202 22-23 0.10739576575038967 2202 24-25 0.16678110855282569 2202 26-27 0.08272836093577496 2202 28-29 0.15741569825355128 2202 30-31 0.1883276387981212 2202 32-33 0.17813985538665378 2202 34-35 0.09012692420706259 2202 36-37 0.10776683937323384 2202 38-39 0.15955000908414263 2202 40-41 0.19157250357272204 2202 42-43 0.3382885386842034 2202 44-45 0.3234141486471245 2202 46-47 0.38854296619472706 2202 48-49 0.2618707711395061 2202 50-51 0.1385518163695849 2202 52-53 0.2222183481995259 2202 54-55 0.16876291592244286 2202 56-57 0.2598356212781212 2202 58-59 0.20629708516057121 2202 60-61 0.16308034848816533 2202 62-63 0.1781726318114707 2202 64-65 0.23249654837123757 2202 66-67 0.1849361003452401 2202 68-69 0.1741862989946199 2202 70-71 0.10142148330518097 2202 72-73 0.17376601451953633 2202 74-75 0.24810854038229024 2202 76-77 -0.1434456154467192 2202 78-79 0.18234470855573193 2202 80-81 0.23762670014973963 2202 82-83 0.26720004464043967 2202 84-85 0.28187019123298285 2202 86-87 0.2903391877822443 2202 88-89 0.4559840116848122 2202 90-91 0.307404729820675 2202 92-93 0.45648013123705056 2202 94-95 0.27132666310969 2202 96-97 0.22274957568319564 2202 98-99 0.37356412464691147 2202 100-101 0.29053743880847094 1102 1 0.061834395858255675 1102 2 0.09210084064607571 1102 3 0.0900012442334912 1102 4 0.07384734687175154 1102 5 0.07843715448771604 1102 6 -0.01918797372775316 1102 7 0.07329921814581297 1102 8 0.09332800520716944 1102 9 0.037093467646521106 1102 10-11 0.0742530374267929 1102 12-13 0.021871233589145334 1102 14-15 0.12134309897658824 1102 16-17 0.078631097001022 1102 18-19 0.07982503606909575 1102 20-21 0.05164836053639732 1102 22-23 0.08484529734243296 1102 24-25 0.07600918990198835 1102 26-27 0.04841478300328106 1102 28-29 0.15313032184484854 1102 30-31 0.1733685115402679 1102 32-33 0.13461353754798466 1102 34-35 0.08008150423796678 1102 36-37 0.08815257206370575 1102 38-39 0.16758352083923 1102 40-41 0.22435597595724488 1102 42-43 0.14241355633558328 1102 44-45 0.08618069895513969 1102 46-47 0.2249690724493334 1102 48-49 0.1291963740901707 1102 50-51 0.13735126128828767 1102 52-53 0.05005955148003949 1102 54-55 0.10922515824866252 1102 56-57 0.13169136638701673 1102 58-59 0.2490489797416089 1102 60-61 0.16462172767226946 1102 62-63 0.12546605840992697 1102 64-65 0.12614992706254924 1102 66-67 0.17422344382910637 1102 68-69 0.09927016845521308 1102 70-71 -0.10684769974555053 1102 72-73 0.02393410210988378 1102 74-75 -0.05407578473078445 1102 76-77 -0.056250519362219364 1102 78-79 0.13740599006597876 1102 80-81 0.17326138552521542 1102 82-83 0.22090203943481868 1102 84-85 0.2181814691112578 1102 86-87 0.29206286282490623 1102 88-89 0.39930487622161337 1102 90-91 0.04107109062700687 1102 92-93 0.21576978174852712 1102 94-95 0.34770807578927077 1102 96-97 0.0658256163589428 1102 98-99 0.1230772081665421 1102 100-101 0.18153959532721942 2305 1 0.06605757770930865 2305 2 0.060193945104288105 2305 3 0.05249071632145785 2305 4 0.03179880116721279 2305 5 0.06844943683499594 2305 6 0.004098233055785272 2305 7 0.008486920608163473 2305 8 0.023142271770005607 2305 9 0.029266475510659973 2305 10-11 0.028013485355316448 2305 12-13 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>>Per sequence quality scores pass #Quality Count 7 4.0 8 42.0 9 165.0 10 282.0 11 214.0 12 164.0 13 261.0 14 509.0 15 1260.0 16 2211.0 17 3706.0 18 5088.0 19 6898.0 20 8820.0 21 11123.0 22 13926.0 23 17332.0 24 21503.0 25 26435.0 26 32538.0 27 41262.0 28 52415.0 29 67497.0 30 79331.0 31 95021.0 32 126083.0 33 178132.0 34 270896.0 35 471441.0 36 953259.0 37 1635862.0 38 511599.0 39 269.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.13307994649922 33.192177131658156 15.775210297387407 17.899532624455215 2 30.1337619629869 25.846199845196296 29.963533976996892 14.05650421481991 3 31.61202821768284 23.70655111289252 25.339502490867826 19.341918178556814 4 27.643815931143557 23.5461758048751 29.306106068092618 19.503902195888724 5 29.667474050532967 21.88222406498649 29.866975813862783 18.583326070617755 6 27.51890391384147 22.198971944633083 31.513469389163912 18.76865475236153 7 30.890091096025756 23.371993990785985 25.059410451579833 20.678504461608423 8 27.221571214449725 26.986151367648443 25.291055124442675 20.501222293459158 9 26.23845120361174 24.1537785823812 28.644358768369997 20.963411445637064 10-11 22.458714978840558 29.215261548922573 31.001954689431756 17.324068782805117 12-13 22.06657120150627 29.026298508827868 30.04592984475622 18.86120044490964 14-15 21.830202168114752 29.003216016747103 30.486201415668656 18.68038039946949 16-17 22.105120891711266 28.39351555277364 30.734453940860423 18.76690961465467 18-19 21.227069593497898 28.369860478200202 31.25262644244003 19.150443485861864 20-21 21.78305563872923 28.76243542295323 30.769986633727015 18.684522304590526 22-23 21.322658104393263 29.80784933068049 30.170472453090035 18.699020111836212 24-25 21.199586327226037 28.941152157199106 30.63130831565114 19.227953199923718 26-27 21.034568081271082 28.883359637307176 30.474714100684537 19.607358180737208 28-29 21.103653764344582 29.694590585622237 30.297345642845247 18.90441000718793 30-31 20.483640769117265 29.639203390839658 30.725612160633435 19.15154367940964 32-33 20.398656210657297 30.065862763151195 30.29305273076667 19.242428295424833 34-35 20.89939528185233 30.110992271032465 30.38161831136254 18.607994135752666 36-37 20.80954639398323 29.7999497142655 30.74404226762991 18.64646162412136 38-39 20.784471459056554 29.129691279244856 31.086862821744116 18.998974439954473 40-41 20.638934840369302 29.35271622868218 29.87567859334105 20.13267033760747 42-43 20.962770035677565 29.515149266321007 30.329943051374574 19.192137646626854 44-45 20.522616141864138 29.21020156269904 30.54440340972522 19.7227788857116 46-47 20.973017483995555 29.405793970190192 30.01697713022319 19.604211415591067 48-49 20.580931205498107 30.028870408069043 30.19802183122353 19.19217655520932 50-51 21.182760021478565 29.354333542092785 29.85032961054029 19.612576825888357 52-53 20.54788861405255 29.241950035012508 29.639470381314926 20.570690969620014 54-55 21.096649603252427 29.477636010556925 29.838979534905995 19.586734851284653 56-57 20.59986178180642 29.236977746784525 30.077172184354506 20.085988287054548 58-59 20.672089399023953 29.582409330882875 30.07997082750522 19.665530442587958 60-61 20.810411850979907 29.70441805504435 29.87271268812732 19.612457405848424 62-63 20.312581739689165 29.723989102848904 29.665797185582164 20.297631971879767 64-65 20.679190343008404 29.549207199894884 29.811424235382805 19.960178221713907 66-67 20.63018043860341 29.870563148116236 29.52980696741242 19.969449445867934 68-69 20.500439085752262 30.057447951616417 29.732319516336798 19.709793446294526 70-71 20.498117178310697 30.61581686752292 29.424609389625665 19.461456564540715 72-73 20.428365905516177 30.512040022709574 29.34552020006176 19.71407387171249 74-75 20.55194982097802 30.046108207282472 29.377169667089788 20.024772304649716 76-77 20.67126332599594 29.784820233933274 29.420853295351545 20.123063144719236 78-79 20.38667999629325 29.60222456752881 29.867015506748583 20.14407992942936 80-81 20.418634449876695 29.80691695283329 29.61413272068703 20.16031587660299 82-83 20.09091998508469 29.92818796745856 29.496109862475596 20.48478218498116 84-85 20.133052805810994 29.92063562266603 29.56953034437184 20.376781227151135 86-87 20.16308486139308 29.77194702174803 29.97266635322589 20.092301763633003 88-89 20.34096482273709 30.20076325682425 29.54890731976974 19.909364600668926 90-91 20.60509516344636 29.87460067603215 29.183356187808762 20.33694797271273 92-93 20.200311119321686 30.14151871809171 29.228274738043403 20.429895424543204 94-95 19.79322536600944 30.494311674594016 29.171752416371717 20.540710543024822 96-97 19.937132819380718 30.649034480384973 29.056855266410302 20.356977433824007 98-99 20.13976431159899 30.25672372297684 28.9290976391776 20.674414326246566 100-101 20.168743970848194 30.138833120845398 28.854358131455417 20.83806477685099 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 68.0 1 54.5 2 53.5 3 96.0 4 115.0 5 89.0 6 69.0 7 61.0 8 59.5 9 75.5 10 89.0 11 93.0 12 109.0 13 112.0 14 136.0 15 190.5 16 241.5 17 300.5 18 432.5 19 641.5 20 927.5 21 1455.0 22 2408.0 23 3859.5 24 5846.5 25 8809.5 26 13422.5 27 19432.0 28 26276.5 29 33811.0 30 41583.0 31 49975.5 32 64329.5 33 87876.0 34 116721.5 35 154023.0 36 201497.0 37 258324.0 38 312206.5 39 328387.5 40 333153.5 41 319988.0 42 284406.5 43 270220.0 44 253828.5 45 238651.0 46 241390.0 47 223727.5 48 177263.0 49 133269.5 50 101424.5 51 81338.0 52 65244.0 53 49371.5 54 38087.5 55 29173.0 56 19930.5 57 12948.0 58 8501.0 59 5813.0 60 4145.0 61 3091.0 62 2151.0 63 1330.5 64 830.5 65 577.5 66 411.5 67 288.0 68 210.0 69 140.5 70 104.0 71 78.5 72 64.5 73 50.5 74 32.0 75 21.0 76 17.5 77 17.0 78 13.0 79 7.5 80 5.0 81 3.0 82 2.0 83 2.0 84 0.5 85 0.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16347581774581993 2 0.0 3 3.2358633758079947E-4 4 0.021097829210268128 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.256002526562124E-4 12-13 0.0 14-15 0.0 16-17 0.0012188418715543448 18-19 0.0 20-21 0.0 22-23 4.09876027602346E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.305382664573854E-4 38-39 6.903175201723723E-4 40-41 0.004832222641206606 42-43 0.0030632839957649024 44-45 9.168279564789319E-4 46-47 0.0041958361772977 48-49 0.007593492721896095 50-51 0.004940084753733539 52-53 0.0013374901953339712 54-55 0.010462624915112517 56-57 0.01827184186206248 58-59 0.022553967729381723 60-61 0.011260804547811823 62-63 5.285243513819725E-4 64-65 0.013957357360985153 66-67 0.034289365572312054 68-69 0.01980348385994493 70-71 0.03620931117529147 72-73 0.029748370634928165 74-75 0.028917832368470786 76-77 0.037708594539415836 78-79 0.01638425489284115 80-81 0.012371784306839235 82-83 9.384003789843186E-4 84-85 0.010160611000037106 86-87 0.020860532562708874 88-89 0.020170215042536504 90-91 0.02487300314871079 92-93 0.042195658420536256 94-95 0.02751562490562065 96-97 0.02876682541093308 98-99 0.03614459390777531 100-101 0.01671862744167464 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4635548.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.38824584153086 #Duplication Level Percentage of deduplicated Percentage of total 1 90.04688152444442 43.57210640468019 2 4.891908412709977 4.734217338169269 3 1.334762034024888 1.937603803270142 4 0.7042948959089559 1.3631837827271178 5 0.4906202019426565 1.1870125473211388 6 0.382474511497037 1.110436241426283 7 0.24283394034413847 0.8225215882827869 8 0.15807756259180625 0.6119276768577842 9 0.10484809873557782 0.45660740198708166 >10 1.320657935759479 12.974531168593748 >50 0.17832079717602867 6.011266851901924 >100 0.11518724119051746 10.519356966392161 >500 0.018167879693928177 6.135014499787522 >1k 0.01092053163877684 7.931381820784865 >5k 0.0 0.0 >10k+ 4.443234192227762E-5 0.6328319078181824 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 29434 0.6349626840235502 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.177381401292792E-4 0.0 0.0 2 6.256002526562124E-4 0.0 0.0 3 0.0011001935477747183 0.0 0.0 4 0.0017905110679470907 0.0 2.1572422505386633E-5 5 0.0022219595180548233 0.0 4.314484501077327E-5 6 0.004336056923582713 0.0 4.314484501077327E-5 7 0.005910843766475938 0.0 4.314484501077327E-5 8 0.007701354834423029 0.0 4.314484501077327E-5 9 0.009038845029757 0.0 4.314484501077327E-5 10-11 0.012716943066925421 0.0 4.314484501077327E-5 12-13 0.01863857304465405 0.0 4.314484501077327E-5 14-15 0.02666351421665788 0.0 4.314484501077327E-5 16-17 0.038226332679545116 0.0 8.628969002154653E-5 18-19 0.05136393798532557 0.0 1.0786211252693316E-4 20-21 0.06613026119026273 0.0 1.294345350323198E-4 22-23 0.08427266851729288 0.0 1.294345350323198E-4 24-25 0.10376335225090971 0.0 1.294345350323198E-4 26-27 0.12391199487094082 0.0 1.294345350323198E-4 28-29 0.14546284495382206 0.0 1.402207462850131E-4 30-31 0.169311158033527 0.0 1.5100695753770643E-4 32-33 0.19281431235314572 0.0 1.6179316879039976E-4 34-35 0.2170940738829584 0.0 1.7257938004309307E-4 36-37 0.24618448563147227 0.0 1.7257938004309307E-4 38-39 0.2798051061061173 0.0 1.7257938004309307E-4 40-41 0.3150760169024245 0.0 1.7257938004309307E-4 42-43 0.3545751225097874 0.0 1.7257938004309307E-4 44-45 0.3960804634101513 0.0 1.7257938004309307E-4 46-47 0.44103739191137703 0.0 1.7257938004309307E-4 48-49 0.4896400598160131 0.0 1.7257938004309307E-4 50-51 0.5410147840125914 0.0 1.7257938004309307E-4 52-53 0.5958518820212843 0.0 1.7257938004309307E-4 54-55 0.6541405676308389 0.0 1.7257938004309307E-4 56-57 0.713572591633179 0.0 1.8336559129578637E-4 58-59 0.7767474309402038 0.0 1.941518025484797E-4 60-61 0.8440102443119993 0.0 1.941518025484797E-4 62-63 0.916331790761308 0.0 1.941518025484797E-4 64-65 0.9906919311373759 1.0786211252693317E-5 1.941518025484797E-4 66-67 1.0688919627194022 2.1572422505386633E-5 1.941518025484797E-4 68-69 1.1486559949330695 2.1572422505386633E-5 1.941518025484797E-4 70-71 1.230232110637189 2.1572422505386633E-5 1.941518025484797E-4 72-73 1.3156696899697726 2.1572422505386633E-5 1.941518025484797E-4 74-75 1.4062738644923964 2.1572422505386633E-5 1.941518025484797E-4 76-77 1.503155613964088 2.1572422505386633E-5 1.941518025484797E-4 78-79 1.6060776417372877 2.1572422505386633E-5 2.3729664755925298E-4 80-81 1.7114049946198377 2.1572422505386633E-5 2.480828588119463E-4 82-83 1.8252642406032684 2.1572422505386633E-5 2.588690700646396E-4 84-85 1.9421328395262005 2.1572422505386633E-5 2.588690700646396E-4 86-87 2.067608835028782 2.1572422505386633E-5 2.588690700646396E-4 88-89 2.199653633184253 2.1572422505386633E-5 2.588690700646396E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGG 25 5.2382413E-5 76.11319 1 CGCGGCT 35 2.7892969E-4 54.283867 4 CGGGTCG 45 1.5033887E-5 52.856377 1 CGCGAGG 75 2.3574103E-9 50.742126 1 CGACGGG 65 2.5646877E-6 43.911453 1 ACGCGGC 45 9.629718E-4 42.22078 3 CGAGCGG 80 2.2382847E-7 41.6244 1 CGAGGCG 105 1.0131771E-9 40.77492 1 CGACGAC 60 8.222235E-5 39.642284 1 GCGCGTT 75 6.9284433E-6 37.998707 4 GCGCACC 155 1.8189894E-12 36.77294 2 CGAGCGA 65 1.317223E-4 36.592876 1 CGAACGG 55 0.0025616656 34.596905 1 CGCTCGG 55 0.0025616656 34.596905 1 CGAGGTG 285 0.0 33.382977 1 GTGTTTG 58745 0.0 32.46423 1 CGGTGCG 60 0.003923491 31.713829 1 CGATCGG 105 1.8813425E-6 31.713827 1 GTATCGC 90 2.3900966E-5 31.713825 1 AACGCGT 120 1.5097976E-7 31.661146 8 >>END_MODULE