##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34271-1.341000000358dd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5243229 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6575791368258 33.0 33.0 33.0 33.0 33.0 2 32.692340349811154 33.0 33.0 33.0 33.0 33.0 3 32.72824475146899 33.0 33.0 33.0 33.0 33.0 4 36.64464455014267 37.0 37.0 37.0 37.0 37.0 5 36.65891381055452 37.0 37.0 37.0 37.0 37.0 6 36.552644753833945 37.0 37.0 37.0 37.0 37.0 7 36.445228693997535 37.0 37.0 37.0 37.0 37.0 8 36.401519750520144 37.0 37.0 37.0 37.0 37.0 9 36.456506477210894 37.0 37.0 37.0 37.0 37.0 10-11 36.38579280058147 37.0 37.0 37.0 37.0 37.0 12-13 36.363821034709716 37.0 37.0 37.0 37.0 37.0 14-15 38.2531700408279 40.0 37.0 40.0 37.0 40.0 16-17 38.26795978203508 40.0 37.0 40.0 37.0 40.0 18-19 38.21473837972745 40.0 37.0 40.0 37.0 40.0 20-21 38.215479907515004 40.0 37.0 40.0 37.0 40.0 22-23 38.02656197163999 40.0 37.0 40.0 35.0 40.0 24-25 38.060079485370565 40.0 37.0 40.0 37.0 40.0 26-27 37.991371061611076 40.0 37.0 40.0 35.0 40.0 28-29 37.809656606644495 40.0 37.0 40.0 33.0 40.0 30-31 37.782990786784254 40.0 37.0 40.0 33.0 40.0 32-33 37.63711407607793 40.0 37.0 40.0 33.0 40.0 34-35 37.4626576485597 40.0 37.0 40.0 33.0 40.0 36-37 37.13857948985253 40.0 37.0 40.0 33.0 40.0 38-39 37.29517841009805 40.0 37.0 40.0 33.0 40.0 40-41 37.07453431845147 38.5 37.0 40.0 33.0 40.0 42-43 36.86335805664792 37.0 37.0 40.0 33.0 40.0 44-45 36.909062430803615 37.0 37.0 40.0 33.0 40.0 46-47 36.80626108453397 37.0 37.0 40.0 33.0 40.0 48-49 36.5965033188518 37.0 37.0 40.0 33.0 40.0 50-51 36.5279620630722 37.0 37.0 40.0 33.0 40.0 52-53 36.42299830886654 37.0 37.0 40.0 33.0 40.0 54-55 36.14843925374993 37.0 37.0 40.0 33.0 40.0 56-57 35.988636296450146 37.0 37.0 40.0 33.0 40.0 58-59 35.671795376475075 37.0 37.0 40.0 27.0 40.0 60-61 35.41992930310692 37.0 33.0 40.0 27.0 40.0 62-63 35.19716037579133 37.0 33.0 38.5 27.0 40.0 64-65 34.916754065099965 37.0 33.0 37.0 27.0 40.0 66-67 34.36250180947657 37.0 33.0 37.0 27.0 40.0 68-69 34.14348925061255 37.0 33.0 37.0 27.0 40.0 70-71 33.69906445055137 37.0 33.0 37.0 27.0 40.0 72-73 32.95691023603966 37.0 33.0 37.0 22.0 37.0 74-75 32.14541106253417 37.0 33.0 37.0 18.5 37.0 76-77 29.52071156914947 33.0 27.0 35.0 18.5 37.0 78-79 31.767283099784503 33.0 33.0 37.0 18.5 37.0 80-81 32.04427662800919 37.0 33.0 37.0 15.0 37.0 82-83 31.892307011576264 37.0 33.0 37.0 15.0 37.0 84-85 31.753313463897918 37.0 33.0 37.0 15.0 37.0 86-87 31.64575436243582 35.0 33.0 37.0 15.0 37.0 88-89 31.381781150508587 33.0 33.0 37.0 15.0 37.0 90-91 31.366421340742505 33.0 33.0 37.0 15.0 37.0 92-93 31.222480078592792 33.0 33.0 37.0 15.0 37.0 94-95 31.040754752462654 33.0 33.0 37.0 15.0 37.0 96-97 30.928601153983546 33.0 33.0 37.0 15.0 37.0 98-99 30.8000421114546 33.0 33.0 37.0 15.0 37.0 100-101 29.215470752850962 33.0 27.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.16609551152116353 2306 2 0.11958021693689602 2306 3 0.13441843707487777 2306 4 0.1774963367616138 2306 5 0.17281278256663057 2306 6 0.16521401877739095 2306 7 0.20834436408720336 2306 8 0.23006931601495495 2306 9 0.18963774447384196 2306 10-11 0.21420626163432388 2306 12-13 0.22491457419852168 2306 14-15 0.4279977775777297 2306 16-17 0.4029208804209645 2306 18-19 0.41128298087415516 2306 20-21 0.38768888182875116 2306 22-23 0.3644251955100728 2306 24-25 0.38746138083160986 2306 26-27 0.39477181114137494 2306 28-29 0.43204473599703164 2306 30-31 0.4383377625620568 2306 32-33 0.4521977907942514 2306 34-35 0.532098844294957 2306 36-37 0.41087305716994393 2306 38-39 0.4724318742033162 2306 40-41 0.5592240107257354 2306 42-43 0.5399511930463845 2306 44-45 0.5114092249264459 2306 46-47 0.6323941851808428 2306 48-49 0.5876944667846189 2306 50-51 0.4941187137824201 2306 52-53 0.5202891362285413 2306 54-55 0.4428920824563676 2306 56-57 0.4442894418323604 2306 58-59 0.5201785592546315 2306 60-61 0.4900090138989128 2306 62-63 0.4710404843200138 2306 64-65 0.47738131803115635 2306 66-67 0.3815516230319318 2306 68-69 0.48869736521456986 2306 70-71 0.37391309774773873 2306 72-73 0.199319916580329 2306 74-75 0.22985050958192943 2306 76-77 -0.11167565007906077 2306 78-79 0.23353827862263543 2306 80-81 0.41701792947089444 2306 82-83 0.42671474253306485 2306 84-85 0.4763558979094462 2306 86-87 0.4267478149319466 2306 88-89 0.5360279632147851 2306 90-91 0.36201745545747954 2306 92-93 0.41564580486664227 2306 94-95 0.3905842105022792 2306 96-97 0.44042822373623025 2306 98-99 0.417140378144385 2306 100-101 0.34033077520929567 2307 1 0.14303135741618433 2307 2 0.14576604311779562 2307 3 0.1269484641074783 2307 4 0.211020293834828 2307 5 0.15709272956114262 2307 6 0.1990531530376458 2307 7 0.1952274457887384 2307 8 0.19351498128384748 2307 9 0.22898787747264038 2307 10-11 0.21706219730136667 2307 12-13 0.2325246604655291 2307 14-15 0.43667056072165167 2307 16-17 0.46657939601353604 2307 18-19 0.3438868668322499 2307 20-21 0.3844012877451348 2307 22-23 0.3613478024706822 2307 24-25 0.4217027578001975 2307 26-27 0.4173933614499461 2307 28-29 0.38986621734971294 2307 30-31 0.42965086236576155 2307 32-33 0.36302254755184293 2307 34-35 0.44038016841725636 2307 36-37 0.3885903161184743 2307 38-39 0.48706279972721944 2307 40-41 0.4399856026914293 2307 42-43 0.3763359863316893 2307 44-45 0.33971732294033785 2307 46-47 0.5861130769810359 2307 48-49 0.48952945971117146 2307 50-51 0.4210454924253 2307 52-53 0.5521826048467702 2307 54-55 0.46409514885427683 2307 56-57 0.5005008080620357 2307 58-59 0.540323468620656 2307 60-61 0.48778268633005695 2307 62-63 0.5762894531532865 2307 64-65 0.5443036712579641 2307 66-67 0.42931931281798086 2307 68-69 0.4497667014593745 2307 70-71 0.283964837055656 2307 72-73 0.21730255372531815 2307 74-75 0.264253641851127 2307 76-77 -0.15341001018852296 2307 78-79 0.19888358918659677 2307 80-81 0.4545046737452765 2307 82-83 0.537496787433497 2307 84-85 0.46482394523627946 2307 86-87 0.38199874895842 2307 88-89 0.5664674998172536 2307 90-91 0.3418936905751835 2307 92-93 0.4041131681072301 2307 94-95 0.45360745873777475 2307 96-97 0.413521743797741 2307 98-99 0.4230145022963363 2307 100-101 0.3093933482044058 2201 1 0.16191579493043662 2201 2 0.1587556060863946 2201 3 0.13724862632953005 2201 4 0.19704100089544596 2201 5 0.14415054438597252 2201 6 0.17582786890702806 2201 7 0.20648103315902233 2201 8 0.20487427619578114 2201 9 0.21184407467559652 2201 10-11 0.18329713911986545 2201 12-13 0.209714673250879 2201 14-15 0.418544712474592 2201 16-17 0.38610906098657694 2201 18-19 0.25799522341579717 2201 20-21 0.33856297006219904 2201 22-23 0.3117056167509489 2201 24-25 0.45701064992404383 2201 26-27 0.42143711360176184 2201 28-29 0.31717727646425686 2201 30-31 0.33781968947190677 2201 32-33 0.47682349305043914 2201 34-35 0.50960499389695 2201 36-37 0.45420934662238466 2201 38-39 0.48844782870754244 2201 40-41 0.42737660502522345 2201 42-43 0.4323320908242323 2201 44-45 0.38069814612799036 2201 46-47 0.5068393791593877 2201 48-49 0.5182702329917888 2201 50-51 0.449277060315616 2201 52-53 0.2992001031661644 2201 54-55 0.451405736006123 2201 56-57 0.5717184355055736 2201 58-59 0.7061406326936464 2201 60-61 0.4752372826113529 2201 62-63 0.6056460772404506 2201 64-65 0.5988731172403234 2201 66-67 0.4177666947259624 2201 68-69 0.46455707438855853 2201 70-71 0.21379115478456612 2201 72-73 0.2317437045155799 2201 74-75 0.23264266980765314 2201 76-77 0.007664322120408684 2201 78-79 0.2538207425133585 2201 80-81 0.42555021632992407 2201 82-83 0.549232593609311 2201 84-85 0.5269841850417301 2201 86-87 0.42336222497575804 2201 88-89 0.6438576126828934 2201 90-91 0.3393581315141958 2201 92-93 0.4186811739982943 2201 94-95 0.4843890773640851 2201 96-97 0.4604790063657127 2201 98-99 0.5150283489556564 2201 100-101 0.4317893394259009 1101 1 0.1643140192103587 1101 2 0.11525988019308642 1101 3 0.12102651374684825 1101 4 0.145116166144021 1101 5 0.135585150884026 1101 6 0.14886592068087623 1101 7 0.14639712587002407 1101 8 0.21798937422160947 1101 9 0.16004181350686508 1101 10-11 0.14350623555542796 1101 12-13 0.12502921270238687 1101 14-15 0.2573599831827025 1101 16-17 0.3075557486364886 1101 18-19 0.3466511099212468 1101 20-21 0.32996808951913437 1101 22-23 0.3411747343753362 1101 24-25 0.3003370800042404 1101 26-27 0.3252997232627237 1101 28-29 0.36924376464850894 1101 30-31 0.4151504046716923 1101 32-33 0.49402646961718943 1101 34-35 0.44544411083853674 1101 36-37 0.47810268778222564 1101 38-39 0.5250127233250979 1101 40-41 0.5160352447558054 1101 42-43 0.46231801633737746 1101 44-45 0.388467692374455 1101 46-47 0.5671398276046915 1101 48-49 0.5298615035060692 1101 50-51 0.47379703551456487 1101 52-53 0.37595803741033507 1101 54-55 0.4328921141494746 1101 56-57 0.3462305089484019 1101 58-59 0.5392044987062974 1101 60-61 0.42427965584736427 1101 62-63 0.49084658361362443 1101 64-65 0.42561566095602643 1101 66-67 0.4609160924304305 1101 68-69 0.4548550669570588 1101 70-71 0.46140017148673707 1101 72-73 0.4652075590748055 1101 74-75 0.45464011261216086 1101 76-77 0.19283038564722332 1101 78-79 0.44740366304646884 1101 80-81 0.6710393322803512 1101 82-83 0.78293691485122 1101 84-85 0.7222597333189817 1101 86-87 0.6929471817941781 1101 88-89 0.9529817732245505 1101 90-91 0.6974826786086616 1101 92-93 0.6624524977269104 1101 94-95 0.8313376779548172 1101 96-97 0.8520469515567122 1101 98-99 0.807540512704275 1101 100-101 0.6662854056178382 2304 1 0.11745749505127634 2304 2 0.1059052571171577 2304 3 0.11553487174449373 2304 4 0.09054512615001187 2304 5 0.09898561733617584 2304 6 0.07891890018580483 2304 7 0.12919988779548675 2304 8 0.12582058272126773 2304 9 0.07350052675823093 2304 10-11 0.06932860829831355 2304 12-13 0.1331855561888986 2304 14-15 0.25398516244190716 2304 16-17 0.21247939476668165 2304 18-19 0.25222211965718344 2304 20-21 0.28516488447208843 2304 22-23 0.24951510470234695 2304 24-25 0.1670805564025457 2304 26-27 0.09654656706654663 2304 28-29 0.1629983879633201 2304 30-31 0.2157110549973993 2304 32-33 0.11546079406181775 2304 34-35 0.19101737400497854 2304 36-37 0.32366441505661925 2304 38-39 0.29509706970683425 2304 40-41 0.2691019606316516 2304 42-43 0.23346642185613575 2304 44-45 0.16235515064020234 2304 46-47 0.2772532615540939 2304 48-49 0.32159231928772414 2304 50-51 0.2431991816881549 2304 52-53 0.24756306120671923 2304 54-55 0.26091843947317983 2304 56-57 0.20510663636039084 2304 58-59 0.28915881299057844 2304 60-61 0.2740673118257604 2304 62-63 0.17248895821976618 2304 64-65 0.0256895245064257 2304 66-67 0.19959275341972926 2304 68-69 0.21714367845368798 2304 70-71 0.11458233586294142 2304 72-73 -0.05443560662080671 2304 74-75 -0.16559861963809652 2304 76-77 -0.13815316908764785 2304 78-79 0.028749369146577664 2304 80-81 0.07905457757382095 2304 82-83 0.19521337335027056 2304 84-85 0.12143001502964879 2304 86-87 0.11469133302723122 2304 88-89 0.3651723843819781 2304 90-91 0.2008548617209911 2304 92-93 0.20710705001485508 2304 94-95 0.23655594220816312 2304 96-97 0.23262901942558045 2304 98-99 0.16731771372062454 2304 100-101 0.14098552219106253 2202 1 0.11666428186877909 2202 2 0.08227488946270967 2202 3 0.07719493894411755 2202 4 0.09754633609857422 2202 5 0.03419922961845856 2202 6 0.07434452832902139 2202 7 0.0791549936507181 2202 8 0.059119322298023746 2202 9 0.0967130484765022 2202 10-11 0.09303185605791242 2202 12-13 0.03624280024924076 2202 14-15 0.020042107207942195 2202 16-17 0.10791074226290931 2202 18-19 -0.033305612819248154 2202 20-21 0.11603986774436947 2202 22-23 0.09413277705893819 2202 24-25 0.09796092586163496 2202 26-27 0.030981958645099894 2202 28-29 0.07270660100977011 2202 30-31 0.160544285882132 2202 32-33 0.14926772314127845 2202 34-35 0.13656649575112567 2202 36-37 0.09256257336942042 2202 38-39 0.1340592459039911 2202 40-41 0.16431591718567518 2202 42-43 0.24387009157071304 2202 44-45 0.2577118966726104 2202 46-47 0.32766797017526983 2202 48-49 0.31245926214916864 2202 50-51 0.19804308499666234 2202 52-53 0.1587399818959483 2202 54-55 0.14275119226442 2202 56-57 0.16149282151305755 2202 58-59 0.24801195499767204 2202 60-61 0.1756576528935483 2202 62-63 0.18721268292897975 2202 64-65 0.10499571145820141 2202 66-67 0.05962616443630964 2202 68-69 0.016496419828435194 2202 70-71 0.009890550686286304 2202 72-73 -0.10461460015905288 2202 74-75 -0.010288755642086755 2202 76-77 -0.3155770780932947 2202 78-79 0.07482458807841041 2202 80-81 0.1334568690909137 2202 82-83 0.21965888762838404 2202 84-85 0.24759172390569617 2202 86-87 0.29640128631177376 2202 88-89 0.38786877613656046 2202 90-91 0.21610554429523532 2202 92-93 0.2746278992776965 2202 94-95 0.26618149672165003 2202 96-97 0.25704843568508906 2202 98-99 0.2482275406241321 2202 100-101 0.2522073647889158 1102 1 0.09651533804809986 1102 2 0.060972023522275265 1102 3 0.06754423018221445 1102 4 0.13292998924837462 1102 5 0.08670028048016576 1102 6 0.019387246490445875 1102 7 0.06517549431668357 1102 8 0.12259314191444304 1102 9 0.09368960539775628 1102 10-11 0.083219972964379 1102 12-13 0.07752672535698224 1102 14-15 0.1524630900042041 1102 16-17 0.1759764477076402 1102 18-19 0.13139442313794802 1102 20-21 0.145855599777164 1102 22-23 0.14175871587092814 1102 24-25 0.08837630743838787 1102 26-27 0.11907101094509898 1102 28-29 0.15216668599030214 1102 30-31 0.15902154928631518 1102 32-33 0.21160256059955884 1102 34-35 0.18498691133527245 1102 36-37 0.08040511630282055 1102 38-39 0.2251455330449872 1102 40-41 0.1936738280219572 1102 42-43 0.06750004644696617 1102 44-45 0.09690524003344336 1102 46-47 0.2679981433268921 1102 48-49 0.03015589989814771 1102 50-51 0.056793850986217365 1102 52-53 0.08858394792400048 1102 54-55 0.05380106641423055 1102 56-57 0.048736373273698064 1102 58-59 0.2248532419614122 1102 60-61 0.15909341359081708 1102 62-63 0.1599278816811136 1102 64-65 0.05652528498560372 1102 66-67 0.24060873279296402 1102 68-69 0.10307007890357767 1102 70-71 -0.07410305298439113 1102 72-73 -0.11803372432279247 1102 74-75 -0.17580390577604277 1102 76-77 -0.2061298313509674 1102 78-79 -0.03667069950318691 1102 80-81 0.03837667441561621 1102 82-83 0.0856906059265441 1102 84-85 0.0881427046255503 1102 86-87 -0.02696392738031861 1102 88-89 0.18840547526599138 1102 90-91 -0.11223594271206494 1102 92-93 -0.023419017266157027 1102 94-95 0.0781805648731968 1102 96-97 -0.011861757420245311 1102 98-99 -0.007965453293532931 1102 100-101 0.06846001726514928 2305 1 0.06723243226898745 2305 2 0.04655439959233121 2305 3 0.03843734863974646 2305 4 0.02733531719578508 2305 5 0.03365410275412728 2305 6 0.07665520072881549 2305 7 0.03943364449597908 2305 8 0.032100554568550876 2305 9 0.07183735354985288 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-0.9564781471307846 1213 98-99 -1.1007429505079394 1213 100-101 -0.8654677096104173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 26.0 9 85.0 10 73.0 11 48.0 12 83.0 13 213.0 14 688.0 15 1674.0 16 3427.0 17 5608.0 18 7548.0 19 9857.0 20 12665.0 21 16703.0 22 21448.0 23 27759.0 24 35606.0 25 46551.0 26 61331.0 27 89101.0 28 147529.0 29 196465.0 30 137311.0 31 118179.0 32 152408.0 33 212582.0 34 322266.0 35 554986.0 36 1058679.0 37 1577267.0 38 424371.0 39 689.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.02507476007432 24.30685271071414 20.452740209362595 15.215332319848947 2 29.60753764521824 28.09640776704584 28.679083061220478 13.616971526515437 3 34.18273649486927 18.74534517111747 30.649119404120874 16.422798929892387 4 28.81967269404581 18.650277841894322 29.016899425621734 23.513150038438134 5 36.825399005078744 17.298386166234586 29.00811694473005 16.868097883956622 6 32.73499974920035 17.892428501597013 32.433468002255864 16.939103746946778 7 30.849596689368326 25.03922678181708 25.549084352409558 18.562092176405038 8 28.234051955388562 30.604633137328165 23.12569220226696 18.035622705016316 9 33.84084502126457 22.591422194224208 24.46618295710525 19.101549827405975 10-11 26.405147233419253 29.339583327373237 27.868517443052482 16.386751996155024 12-13 22.78193456742019 28.08974202728891 27.569976440090638 21.55834696520026 14-15 22.33242149065013 28.373889448658453 27.737316069925612 21.556372990765805 16-17 25.582156470751137 24.888474127714147 28.12704432311854 21.402325078416183 18-19 21.259065739833222 24.85268142970677 31.857744531089526 22.03050829937048 20-21 24.79073486967668 25.168688989170608 31.405151291313043 18.63542484983967 22-23 21.138501188348606 32.41701323456923 27.519823054640003 18.924662522442162 24-25 21.03579683435532 24.850659774730417 31.45830555941768 22.65523783149658 26-27 20.960213639343237 25.09935194514678 27.77303833191341 26.16739608359658 28-29 24.338294207634263 29.229631206266216 27.28275839182305 19.149316194276466 30-31 20.512932011933867 26.171401249115767 30.745910582963287 22.569756155987083 32-33 20.286869026700913 29.864335507756767 27.263276122404722 22.585519343137598 34-35 24.072961528096524 29.925519179116534 27.420679127308762 18.580840165478183 36-37 20.27114798561982 29.936039873447235 31.31332203677209 18.479490104160856 38-39 20.4159562617873 26.138754505720797 31.06476765934057 22.380521573151334 40-41 24.003081833376154 25.97829327312333 30.799804233582297 19.21882065991822 42-43 20.54754663696147 29.4888585017371 30.676824693736705 19.286770167564722 44-45 20.259825292947387 25.947102300761543 30.917466642838875 22.875605763452196 46-47 23.894369566521405 25.980182587673067 27.352923141272424 22.772524704533108 48-49 20.320775291355105 29.75515699634682 30.640280276163878 19.283787436134194 50-51 23.895748491624364 26.23259945284876 30.185508245367203 19.686143810159674 52-53 20.30685751885794 26.018671981537818 26.866959747480045 26.8075107521242 54-55 24.023999909206854 26.078633540266043 30.508537941377956 19.388828609149144 56-57 20.543901276101238 26.043155271017355 30.298498366113908 23.114445086767503 58-59 20.50102512326994 26.294099496332784 30.12694931994672 23.077926060450558 60-61 23.859840033265602 26.283899228491688 30.097884610891146 19.758376127351564 62-63 20.21475434848947 26.32415318889228 30.23322589258468 23.227866570033566 64-65 24.123013160143568 26.332447368581686 29.968654244085258 19.57588522718949 66-67 20.742188535917006 33.17407021097865 26.41926412844126 19.664477124663094 68-69 20.866610182699212 33.24436297730598 26.154480503445548 19.734546336549258 70-71 20.699112829886545 33.46716390701327 26.131724046680148 19.701999216420035 72-73 20.437966648555257 33.46470145176159 26.153543400223445 19.94378849945971 74-75 20.748503725138715 32.76581533720842 26.080120434577896 20.405560503074966 76-77 20.757538610066234 32.12007399090091 26.27103412682437 20.851353272208478 78-79 20.665295909723085 31.656864781675576 26.700823045502986 20.97701626309835 80-81 20.557114848858333 31.324348510366505 26.87473718815816 21.243799452616997 82-83 20.20863495548596 31.30956669973186 26.965292226449726 21.516506118332455 84-85 20.305806460227043 31.043671241603793 26.996468419391174 21.654053878777997 86-87 20.18769061284638 30.980139303862213 27.476167879991703 21.356002203299703 88-89 20.217952536845328 31.054812333220877 27.50020244554215 21.227032684391645 90-91 20.542480973152088 30.561338513354325 27.25158743860807 21.644593074885517 92-93 20.313616649696254 30.820440319943703 27.051196201782318 21.81474682857772 94-95 20.24911661384092 30.80446994731924 26.947684767068786 21.998728671771055 96-97 20.49869560311079 30.996092961922905 26.79654451437412 21.708666920592187 98-99 20.646253237147356 30.759298567751596 26.824858200854386 21.76958999424666 100-101 20.688599121618324 30.805315460494185 26.390001617589608 22.11608380029788 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2209.0 1 1329.5 2 559.0 3 843.0 4 874.0 5 616.5 6 537.5 7 575.0 8 565.5 9 548.5 10 525.5 11 516.5 12 527.5 13 527.0 14 530.0 15 542.5 16 591.5 17 662.5 18 720.0 19 821.5 20 943.5 21 1166.5 22 1594.0 23 2292.5 24 3334.0 25 5076.0 26 8379.5 27 13222.5 28 18201.0 29 23224.5 30 28845.0 31 34009.5 32 44722.0 33 65699.0 34 97308.0 35 133265.5 36 179135.5 37 239629.5 38 287828.5 39 304303.5 40 312119.0 41 315173.5 42 311487.5 43 310901.0 44 298020.5 45 276809.5 46 255983.0 47 225886.0 48 193181.0 49 170377.0 50 151395.0 51 136990.5 52 125781.0 53 110543.5 54 92608.0 55 76438.0 56 63551.0 57 53951.0 58 47102.5 59 40392.5 60 33143.5 61 27387.0 62 22528.0 63 18050.0 64 14681.0 65 11732.0 66 8991.5 67 7176.5 68 6421.0 69 5252.0 70 3615.5 71 2603.0 72 1871.0 73 1367.0 74 1040.5 75 807.5 76 651.0 77 491.0 78 337.0 79 208.5 80 137.0 81 94.0 82 61.5 83 53.5 84 39.5 85 28.5 86 23.5 87 16.5 88 9.0 89 6.0 90 4.0 91 3.5 92 2.0 93 1.5 94 1.0 95 0.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1617896147583865 2 0.0 3 3.432999016445782E-4 4 0.019816033211595373 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.5773320219277087E-4 12-13 0.0 14-15 0.0 16-17 9.726830546596382E-4 18-19 0.0 20-21 0.0 22-23 5.435581776039154E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.724247787003009E-4 38-39 6.38919261394076E-4 40-41 0.0046917653224759015 42-43 0.002870368622083834 44-45 9.917552714176704E-4 46-47 0.004071918277839858 48-49 0.007628886703212848 50-51 0.004853879164919175 52-53 0.0015067051238845375 54-55 0.010346677591232426 56-57 0.018547730797186236 58-59 0.02243846301582479 60-61 0.010995132961005518 62-63 5.530942859829315E-4 64-65 0.013979934883637544 66-67 0.03429184573094175 68-69 0.019711136019426197 70-71 0.036246747948640044 72-73 0.030458330162577294 74-75 0.029466574891159624 76-77 0.038983611053417654 78-79 0.016592828579487948 80-81 0.0123492603508258 82-83 9.917552714176704E-4 84-85 0.010184563748789153 86-87 0.02081732459139206 88-89 0.020569385773537643 90-91 0.024679448484893562 92-93 0.0428075905134031 94-95 0.02797894198403312 96-97 0.029266316615200287 98-99 0.036704481150832816 100-101 0.016421178628665656 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5243229.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.35736934415197 #Duplication Level Percentage of deduplicated Percentage of total 1 90.65357238925925 48.370361443405244 2 4.781336290490675 5.102390528206168 3 1.420208896417974 2.2733583189607294 4 0.8034233339845727 1.7147422228449924 5 0.39587461065712937 1.0561413907402406 6 0.2682725580928579 0.8588590780236653 7 0.238999089004501 0.892665386545031 8 0.16279313570407636 0.6948970774764045 9 0.13793370995143267 0.6623801916198951 >10 0.9110794463773962 9.739911323363891 >50 0.11081442926278248 4.200958352655654 >100 0.10016427535642633 11.443570403685301 >500 0.01303997118834846 4.594152116156481 >1k 0.0024165569125762826 1.9504197523763276 >5k 3.565430319676323E-5 0.11216667284533127 >10k+ 3.5653036653534624E-5 6.3330257410948585 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 332905 6.349236319832683 TruSeq Adapter, Index 7 (97% over 35bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5896 0.11244979000535739 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0026510381293664647 0.0 0.0 2 0.002994338031011043 0.0 0.0 3 0.003471143449961846 0.0 1.907221675803212E-5 4 0.00377629891809036 0.0 1.907221675803212E-5 5 0.004386609854347388 0.0 3.814443351606424E-5 6 0.005130426307910641 0.0 3.814443351606424E-5 7 0.006236614879876504 0.0 3.814443351606424E-5 8 0.007323731235084335 0.0 3.814443351606424E-5 9 0.008220125422711845 0.0 3.814443351606424E-5 10-11 0.012034568774318268 0.0 3.814443351606424E-5 12-13 0.015677362175102405 0.0 3.814443351606424E-5 14-15 0.02011165257134487 0.0 3.814443351606424E-5 16-17 0.02476527346030471 0.0 5.7216650274096366E-5 18-19 0.030439257945819264 0.0 7.628886703212848E-5 20-21 0.036008345239164644 0.0 8.582497541114454E-5 22-23 0.04174908248333231 0.0 9.536108379016061E-5 24-25 0.047556572486153095 0.0 9.536108379016061E-5 26-27 0.053955301208472875 0.0 9.536108379016061E-5 28-29 0.060649649290542146 0.0 9.536108379016061E-5 30-31 0.08760822767802055 0.0 9.536108379016061E-5 32-33 0.0965435612291586 0.0 9.536108379016061E-5 34-35 0.10475415054349144 0.0 1.0489719216917667E-4 36-37 0.11302195650809835 0.0 1.1443330054819273E-4 38-39 0.12282507592172687 0.0 1.1443330054819273E-4 40-41 0.13348644508946683 0.0 1.1443330054819273E-4 42-43 0.14434807253316612 0.0 1.1443330054819273E-4 44-45 0.1565733634750647 0.0 1.1443330054819273E-4 46-47 0.1696473680626957 0.0 1.1443330054819273E-4 48-49 0.18454276935071878 0.0 1.1443330054819273E-4 50-51 0.19937141788008877 0.0 1.1443330054819273E-4 52-53 0.21533486330656165 0.0 1.1443330054819273E-4 54-55 0.23333903592614397 0.0 1.1443330054819273E-4 56-57 0.25245893322607116 0.0 1.1443330054819273E-4 58-59 0.27357187717721276 0.0 1.1443330054819273E-4 60-61 0.29620106236061783 0.0 1.1443330054819273E-4 62-63 0.32095679971254354 1.907221675803212E-5 1.1443330054819273E-4 64-65 0.3479821308586751 1.907221675803212E-5 1.1443330054819273E-4 66-67 0.37730566412414945 1.907221675803212E-5 1.1443330054819273E-4 68-69 0.4085936357157011 1.907221675803212E-5 1.1443330054819273E-4 70-71 0.44225609829362783 1.907221675803212E-5 1.1443330054819273E-4 72-73 0.47854099067578393 1.907221675803212E-5 1.1443330054819273E-4 74-75 0.5177057877884029 1.907221675803212E-5 1.3350551730622484E-4 76-77 0.561085544804547 1.907221675803212E-5 1.3350551730622484E-4 78-79 0.6093954698526423 1.907221675803212E-5 1.3350551730622484E-4 80-81 0.6633030905192201 1.907221675803212E-5 1.3350551730622484E-4 82-83 0.7210346906457834 1.907221675803212E-5 1.430416256852409E-4 84-85 0.7857753304309234 1.907221675803212E-5 1.5257773406425696E-4 86-87 0.8562948518937472 1.907221675803212E-5 1.5257773406425696E-4 88-89 0.9349009932619765 1.907221675803212E-5 1.5257773406425696E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 44320 0.0 80.393326 3 GATCGGA 44510 0.0 80.3191 1 ATCGGAA 44630 0.0 79.92006 2 CGGAAGA 44510 0.0 79.8901 4 GAAGAGC 46080 0.0 77.13724 6 GGAAGAG 50175 0.0 71.07834 5 GAGCACA 52355 0.0 67.80126 9 AGAGCAC 52540 0.0 67.643875 8 AAGAGCA 54500 0.0 65.106606 7 ATGCCGT 38755 0.0 45.35002 48-49 GCCGTCT 38875 0.0 45.319927 50-51 TGCCGTC 38920 0.0 45.06005 50-51 TCGTATG 39185 0.0 45.033962 44-45 TATGCCG 38970 0.0 44.996227 48-49 CGTATGC 38915 0.0 44.91315 46-47 TCTCGTA 38915 0.0 44.900948 42-43 GTATGCC 39295 0.0 44.877674 46-47 TATCTCG 38670 0.0 44.821823 40-41 CGTATCT 38845 0.0 44.760498 38-39 TCGTATC 38740 0.0 44.642754 38-39 >>END_MODULE