FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34271-1.341000000358dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34271-1.341000000358dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5243229
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3329056.349236319832683TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58960.11244979000535739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG443200.080.3933263
GATCGGA445100.080.31911
ATCGGAA446300.079.920062
CGGAAGA445100.079.89014
GAAGAGC460800.077.137246
GGAAGAG501750.071.078345
GAGCACA523550.067.801269
AGAGCAC525400.067.6438758
AAGAGCA545000.065.1066067
ATGCCGT387550.045.3500248-49
GCCGTCT388750.045.31992750-51
TGCCGTC389200.045.0600550-51
TCGTATG391850.045.03396244-45
TATGCCG389700.044.99622748-49
CGTATGC389150.044.9131546-47
TCTCGTA389150.044.90094842-43
GTATGCC392950.044.87767446-47
TATCTCG386700.044.82182340-41
CGTATCT388450.044.76049838-39
TCGTATC387400.044.64275438-39
Produced by FastQC (version 0.11.2)