##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34211-2.34100000035863.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3527945 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66358914325478 33.0 33.0 33.0 33.0 33.0 2 32.70324225576079 33.0 33.0 33.0 33.0 33.0 3 32.73207320408907 33.0 33.0 33.0 33.0 33.0 4 36.64286489727022 37.0 37.0 37.0 37.0 37.0 5 36.65098265420804 37.0 37.0 37.0 37.0 37.0 6 36.546207494731355 37.0 37.0 37.0 37.0 37.0 7 36.47188349024716 37.0 37.0 37.0 37.0 37.0 8 36.44424360357092 37.0 37.0 37.0 37.0 37.0 9 36.43338912596427 37.0 37.0 37.0 37.0 37.0 10-11 36.397391966144596 37.0 37.0 37.0 37.0 37.0 12-13 36.36604794008977 37.0 37.0 37.0 37.0 37.0 14-15 38.2441825198522 40.0 37.0 40.0 37.0 40.0 16-17 38.22711422655399 40.0 37.0 40.0 37.0 40.0 18-19 38.22119619211751 40.0 37.0 40.0 37.0 40.0 20-21 38.174081653767274 40.0 37.0 40.0 37.0 40.0 22-23 38.04989972916245 40.0 37.0 40.0 35.0 40.0 24-25 38.0306172573552 40.0 37.0 40.0 35.0 40.0 26-27 37.9653607128229 40.0 37.0 40.0 33.0 40.0 28-29 37.80301733728842 40.0 37.0 40.0 33.0 40.0 30-31 37.734187040897744 40.0 37.0 40.0 33.0 40.0 32-33 37.590843819844125 40.0 37.0 40.0 33.0 40.0 34-35 37.499794781381226 40.0 37.0 40.0 33.0 40.0 36-37 37.45634073093543 40.0 37.0 40.0 33.0 40.0 38-39 37.313035775784485 40.0 37.0 40.0 33.0 40.0 40-41 37.201899406028154 40.0 37.0 40.0 33.0 40.0 42-43 37.03736509497739 40.0 37.0 40.0 33.0 40.0 44-45 36.93392527377836 40.0 37.0 40.0 33.0 40.0 46-47 36.820843153733975 38.5 37.0 40.0 33.0 40.0 48-49 36.72008449678212 37.0 37.0 40.0 33.0 40.0 50-51 36.61378918889042 37.0 37.0 40.0 33.0 40.0 52-53 36.48015119283322 37.0 37.0 40.0 33.0 40.0 54-55 36.27734332593053 37.0 37.0 40.0 33.0 40.0 56-57 36.09107469079025 37.0 37.0 40.0 30.0 40.0 58-59 35.87295252618734 37.0 37.0 40.0 27.0 40.0 60-61 35.661559633157545 37.0 35.0 40.0 27.0 40.0 62-63 35.45101425900914 37.0 33.0 40.0 27.0 40.0 64-65 35.20950312434009 37.0 33.0 40.0 27.0 40.0 66-67 34.86691969404285 37.0 33.0 40.0 27.0 40.0 68-69 34.61256142031692 37.0 33.0 37.0 27.0 40.0 70-71 34.28162981565755 37.0 33.0 37.0 27.0 40.0 72-73 33.868385419840735 37.0 33.0 37.0 27.0 40.0 74-75 33.39482049748508 37.0 33.0 37.0 24.5 40.0 76-77 30.686042015961135 33.0 30.0 35.0 22.0 37.0 78-79 32.95341565699012 37.0 33.0 37.0 24.5 37.0 80-81 33.21454274372191 37.0 33.0 37.0 27.0 37.0 82-83 33.019207640708686 37.0 33.0 37.0 22.0 37.0 84-85 32.83183156199998 37.0 33.0 37.0 22.0 37.0 86-87 32.69055583349514 37.0 33.0 37.0 22.0 37.0 88-89 32.38407628236835 37.0 33.0 37.0 22.0 37.0 90-91 32.31556415987211 35.0 33.0 37.0 22.0 37.0 92-93 32.13482168797983 33.0 33.0 37.0 22.0 37.0 94-95 31.9339558865005 33.0 33.0 37.0 22.0 37.0 96-97 31.81275572606716 33.0 33.0 37.0 22.0 37.0 98-99 31.67080042347599 33.0 33.0 37.0 18.5 37.0 100-101 29.96791007229421 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.16010500911907855 2306 2 0.15420521309803092 2306 3 0.1186907882343533 2306 4 0.15717917049405372 2306 5 0.1458897640533081 2306 6 0.17129567258184863 2306 7 0.21789188372423496 2306 8 0.21657853183569387 2306 9 0.24170554179681147 2306 10-11 0.24623915343821778 2306 12-13 0.2590929514121427 2306 14-15 0.4239953922069901 2306 16-17 0.4777743834621475 2306 18-19 0.4397661180019412 2306 20-21 0.43959270147207263 2306 22-23 0.44601712141857774 2306 24-25 0.3885882396187199 2306 26-27 0.303735174073978 2306 28-29 0.42409229957485906 2306 30-31 0.3726433083264311 2306 32-33 0.3416122207874608 2306 34-35 0.40603182153262196 2306 36-37 0.29078299391498774 2306 38-39 0.4849577059914836 2306 40-41 0.43465749046376345 2306 42-43 0.4562561878217295 2306 44-45 0.378789840229139 2306 46-47 0.5322926256364156 2306 48-49 0.33301307231587174 2306 50-51 0.31747876115476004 2306 52-53 0.29300627069724783 2306 54-55 0.2990015029112598 2306 56-57 0.3772999652846565 2306 58-59 0.3532137291602311 2306 60-61 0.482794819043626 2306 62-63 0.29840489585792085 2306 64-65 0.4131907267777777 2306 66-67 0.2851161081762754 2306 68-69 0.2879761835929884 2306 70-71 0.3452406594389572 2306 72-73 0.26791973915574374 2306 74-75 0.2420928444218049 2306 76-77 -0.16589236519536144 2306 78-79 0.018090240595988405 2306 80-81 0.30302344091619915 2306 82-83 0.39403308707635887 2306 84-85 0.38103506785112984 2306 86-87 0.29074767376240374 2306 88-89 0.4380744933156464 2306 90-91 0.1740170889616337 2306 92-93 0.3067470641112564 2306 94-95 0.2604193927471812 2306 96-97 0.11702232630247877 2306 98-99 0.24412276803207433 2306 100-101 -0.01718619819051881 2307 1 0.1721232204556813 2307 2 0.13658395292608816 2307 3 0.11966389674130795 2307 4 0.17983696328690968 2307 5 0.15507646523688834 2307 6 0.214096383100987 2307 7 0.24730182156808667 2307 8 0.24485400935852653 2307 9 0.23235998761044385 2307 10-11 0.19742612449341124 2307 12-13 0.18670417350219992 2307 14-15 0.4674356199272296 2307 16-17 0.4000590323363227 2307 18-19 0.4141413437873922 2307 20-21 0.46448925068509084 2307 22-23 0.37342433341629544 2307 24-25 0.32579079183316395 2307 26-27 0.369516202527592 2307 28-29 0.3597355935507238 2307 30-31 0.41946050100477095 2307 32-33 0.43572316515250265 2307 34-35 0.3862030400815115 2307 36-37 0.3463146124450347 2307 38-39 0.39417803403542706 2307 40-41 0.40948821384821343 2307 42-43 0.4943018389619809 2307 44-45 0.5486472509180587 2307 46-47 0.516403398654397 2307 48-49 0.46859875084309266 2307 50-51 0.48305285233709583 2307 52-53 0.43749610624691115 2307 54-55 0.42365202630512755 2307 56-57 0.41575649239391055 2307 58-59 0.5749489608285998 2307 60-61 0.505011928618643 2307 62-63 0.40149174882643734 2307 64-65 0.34333699291116915 2307 66-67 0.21786702809580305 2307 68-69 0.18642056647941985 2307 70-71 0.12301244800239886 2307 72-73 0.18521568979637237 2307 74-75 -0.0436035671424051 2307 76-77 -0.3464787790870396 2307 78-79 -0.10907462240489707 2307 80-81 0.2877771223791612 2307 82-83 0.3562075098152562 2307 84-85 0.3147223280544935 2307 86-87 0.33461966037448576 2307 88-89 0.5469466298564072 2307 90-91 0.3043934599165965 2307 92-93 0.33081418646590777 2307 94-95 0.317831009792215 2307 96-97 0.28392185838221806 2307 98-99 0.19240451400149894 2307 100-101 0.12246129807197548 2201 1 0.11858120188203003 2201 2 0.11264079906121793 2201 3 0.08145541215536412 2201 4 0.1639679212099665 2201 5 0.15191440072837992 2201 6 0.13213129696259074 2201 7 0.09818268490698756 2201 8 0.18740837239574404 2201 9 0.17881832065076964 2201 10-11 0.20456933827330914 2201 12-13 0.20523880115003834 2201 14-15 0.3615036459804202 2201 16-17 0.40470227072919585 2201 18-19 0.2451393156779602 2201 20-21 0.34388653510001177 2201 22-23 0.4912696921821009 2201 24-25 0.46017290762105034 2201 26-27 0.430306215846727 2201 28-29 0.45417787752261063 2201 30-31 0.31654713306324567 2201 32-33 0.3816589998207931 2201 34-35 0.3361310998615181 2201 36-37 0.45224904768969054 2201 38-39 0.5607351143454693 2201 40-41 0.488066378338857 2201 42-43 0.48827429633581687 2201 44-45 0.4483254538049479 2201 46-47 0.5362203624817496 2201 48-49 0.47813118160677703 2201 50-51 0.4019534398137381 2201 52-53 0.43392687275132147 2201 54-55 0.40801557687495205 2201 56-57 0.23017234905917405 2201 58-59 0.4718715649661007 2201 60-61 0.3750868716220239 2201 62-63 0.539013425828216 2201 64-65 0.5389642863133446 2201 66-67 0.4487719277662663 2201 68-69 0.41199464662208385 2201 70-71 0.20506086665369594 2201 72-73 0.4066493395353987 2201 74-75 0.3670641548350133 2201 76-77 0.3107283581141438 2201 78-79 0.48857139233194147 2201 80-81 0.43680519101983606 2201 82-83 0.6714308479821156 2201 84-85 0.617332921199143 2201 86-87 0.4911778939783815 2201 88-89 0.685772970525349 2201 90-91 0.48607293002027063 2201 92-93 0.6190965099873367 2201 94-95 0.619822762334735 2201 96-97 0.42815180267249886 2201 98-99 0.5951934457560348 2201 100-101 0.4509447025954181 1101 1 0.10234952060645242 1101 2 0.10834317053549114 1101 3 0.09249358654523121 1101 4 0.12426747718374287 1101 5 0.13806646905874942 1101 6 0.1481796371578028 1101 7 0.12351673014647702 1101 8 0.08727511088414985 1101 9 0.21809181031945002 1101 10-11 0.13608229348222523 1101 12-13 0.13879643541266518 1101 14-15 0.28111994244641636 1101 16-17 0.33898254023112173 1101 18-19 0.2734311953090156 1101 20-21 0.29480824214746804 1101 22-23 0.27201348264691916 1101 24-25 0.3207672609051144 1101 26-27 0.2347098413929274 1101 28-29 0.2762941921333777 1101 30-31 0.3536178474970484 1101 32-33 0.27083870468899107 1101 34-35 0.31410910650534163 1101 36-37 0.25989388162085447 1101 38-39 0.36649170870758496 1101 40-41 0.3109863485372486 1101 42-43 0.30879663223371523 1101 44-45 0.32992111761483756 1101 46-47 0.26187347169086905 1101 48-49 0.32314525090465906 1101 50-51 0.35540650023708054 1101 52-53 0.2705166402994408 1101 54-55 0.2655570329439385 1101 56-57 0.34629375745947755 1101 58-59 0.4292495760909034 1101 60-61 0.3718759571160817 1101 62-63 0.3274509148821849 1101 64-65 0.3989550498358554 1101 66-67 0.42239708266739484 1101 68-69 0.477568745607968 1101 70-71 0.23259539930907636 1101 72-73 0.1763232417057381 1101 74-75 0.07579316251798218 1101 76-77 -0.18383120832995914 1101 78-79 0.20013903114811882 1101 80-81 0.4293132938435704 1101 82-83 0.5475534176477694 1101 84-85 0.554680051501002 1101 86-87 0.5045987135098571 1101 88-89 0.5837333246734175 1101 90-91 0.4324364338481814 1101 92-93 0.5702357965096851 1101 94-95 0.641716401156593 1101 96-97 0.5669988271035642 1101 98-99 0.5721828779256732 1101 100-101 0.4293906076676528 2304 1 0.11735740147884144 2304 2 0.10968996721821611 2304 3 0.09768154460481782 2304 4 0.14029390954554088 2304 5 0.1325847607496229 2304 6 0.13216692128939656 2304 7 0.1565278481113097 2304 8 0.05654405438006904 2304 9 1.7339819504513798E-4 2304 10-11 0.039612090459115734 2304 12-13 0.10696011473571332 2304 14-15 0.2352679458546305 2304 16-17 0.24879000955439068 2304 18-19 0.16939451278825857 2304 20-21 0.2183133277071505 2304 22-23 0.24509825953932562 2304 24-25 0.22738394792923344 2304 26-27 0.13987380237941238 2304 28-29 0.17346985749874477 2304 30-31 0.27226145470122276 2304 32-33 0.20363705403803323 2304 34-35 0.29657881682115317 2304 36-37 0.20014062639819485 2304 38-39 0.19677902986013152 2304 40-41 0.1667663837872837 2304 42-43 0.2914886583879408 2304 44-45 0.23332515743449989 2304 46-47 0.3125783229022261 2304 48-49 0.2411363061212839 2304 50-51 0.3494421343288394 2304 52-53 0.2142723628612302 2304 54-55 0.35785008840556287 2304 56-57 0.31049472826126845 2304 58-59 0.3587532508888671 2304 60-61 0.22308086278598438 2304 62-63 0.21173348388089863 2304 64-65 0.17809180164527305 2304 66-67 0.1815165815726516 2304 68-69 0.18950785306895312 2304 70-71 0.10112240273874562 2304 72-73 0.05105763742901814 2304 74-75 0.005162456422780792 2304 76-77 0.02234120011516083 2304 78-79 0.1932782041223362 2304 80-81 0.2788436329614612 2304 82-83 0.1912605756745407 2304 84-85 0.27150243431937326 2304 86-87 0.2538950302375369 2304 88-89 0.5483672477616395 2304 90-91 0.32990965205532063 2304 92-93 0.28521005160223467 2304 94-95 0.3273422494402283 2304 96-97 0.24629485320455657 2304 98-99 0.2477302271250501 2304 100-101 0.18476631871844873 2202 1 0.11671313254774418 2202 2 0.1152502427977069 2202 3 0.059851051109312436 2202 4 0.08443199617372699 2202 5 0.06547511362418845 2202 6 0.01212129775921511 2202 7 0.1324091128985856 2202 8 0.06956767495273652 2202 9 0.1336357409647988 2202 10-11 0.15919574011051196 2202 12-13 0.1367459711955945 2202 14-15 0.16004411063214974 2202 16-17 0.15757532852956047 2202 18-19 0.08883773319409727 2202 20-21 0.1883954962650165 2202 22-23 0.15465244952382307 2202 24-25 0.08731640475534874 2202 26-27 0.09615979290326493 2202 28-29 0.17903424961632908 2202 30-31 0.2177361873904431 2202 32-33 0.16477957645103913 2202 34-35 0.23438056827907872 2202 36-37 0.11214685994279705 2202 38-39 0.22851729274987065 2202 40-41 0.2557580728040918 2202 42-43 0.22833955098857217 2202 44-45 0.28718059641351346 2202 46-47 0.363624746884021 2202 48-49 0.33447008264807465 2202 50-51 0.22951693489793712 2202 52-53 0.19877513298415295 2202 54-55 0.16249387514873348 2202 56-57 0.3130533102847082 2202 58-59 0.3132749054766677 2202 60-61 0.2299270342703963 2202 62-63 0.2941531505617405 2202 64-65 0.2558501340713306 2202 66-67 0.18546587762006794 2202 68-69 -0.028986069077603815 2202 70-71 -0.12525069618867235 2202 72-73 0.027581880889187005 2202 74-75 0.08960788992865076 2202 76-77 -0.27341356775755443 2202 78-79 0.19782335369767168 2202 80-81 0.25609343161973896 2202 82-83 0.20388421620319974 2202 84-85 0.25415233221693967 2202 86-87 0.3318970371420136 2202 88-89 0.4896250479869053 2202 90-91 0.17529364887219856 2202 92-93 0.3135684523996858 2202 94-95 0.21330603422712358 2202 96-97 0.20298948693466556 2202 98-99 0.30649128924595104 2202 100-101 0.277466947111062 1102 1 0.11223183761860867 1102 2 0.06433676785303533 1102 3 0.09409159522338939 1102 4 0.09591846202147991 1102 5 0.04707314216972236 1102 6 0.004692324127056224 1102 7 0.10090067850200768 1102 8 0.09307728474221477 1102 9 0.12679490171061758 1102 10-11 0.049720251530352755 1102 12-13 0.03171115992465445 1102 14-15 0.11245936459573613 1102 16-17 0.10622907339200793 1102 18-19 0.12154728863698949 1102 20-21 0.14799629184485497 1102 22-23 0.12836693743471983 1102 24-25 0.1141720962637578 1102 26-27 0.08859090197120878 1102 28-29 0.10324511002341552 1102 30-31 0.13706740459321765 1102 32-33 0.1463559824091547 1102 34-35 0.11003213347522944 1102 36-37 0.034996756378809835 1102 38-39 0.18244804375871837 1102 40-41 0.07459461994729821 1102 42-43 0.1633837108809857 1102 44-45 0.12262931217962603 1102 46-47 0.10569289755721911 1102 48-49 0.11078923291432119 1102 50-51 0.055324440004973496 1102 52-53 0.08948423013767126 1102 54-55 0.07948908653936115 1102 56-57 0.0892425608042231 1102 58-59 0.2426066514748797 1102 60-61 0.0916762610849915 1102 62-63 0.17957987697754163 1102 64-65 0.08275617610105002 1102 66-67 0.17914118868699092 1102 68-69 0.10918527531196531 1102 70-71 0.03357235408072512 1102 72-73 0.10456464204871452 1102 74-75 0.045850615570202535 1102 76-77 0.09502880782799039 1102 78-79 0.2950068666225647 1102 80-81 0.24911667113878622 1102 82-83 0.41066129345780666 1102 84-85 0.33179389803385106 1102 86-87 0.3065529013464712 1102 88-89 0.5051420188715241 1102 90-91 0.1459823978024275 1102 92-93 0.3797953174628148 1102 94-95 0.42552882855077456 1102 96-97 0.253656573864145 1102 98-99 0.11590372745598287 1102 100-101 0.15819128079871447 2305 1 0.09317238151260909 2305 2 0.0877115888142086 2305 3 0.08049381326466687 2305 4 0.07762983651064559 2305 5 0.09351326138204996 2305 6 0.07628106752341779 2305 7 0.010290593303579953 2305 8 0.014965811059852285 2305 9 0.11004966549457862 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-1.0003232395711343 1213 92-93 -1.1630530093458873 1213 94-95 -1.0124573126082943 1213 96-97 -1.0203261757430475 1213 98-99 -1.1356456200039382 1213 100-101 -0.9225624055941815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 6.0 8 45.0 9 174.0 10 259.0 11 181.0 12 137.0 13 259.0 14 592.0 15 1159.0 16 2367.0 17 3719.0 18 5384.0 19 7213.0 20 9456.0 21 11822.0 22 15088.0 23 18924.0 24 23495.0 25 28159.0 26 34934.0 27 43545.0 28 56229.0 29 76312.0 30 84968.0 31 87421.0 32 111135.0 33 151346.0 34 221777.0 35 353660.0 36 613367.0 37 1042164.0 38 520627.0 39 2020.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.01386106905209 29.18041648791781 16.04688250159632 17.75883994143378 2 31.013493691086452 27.064821021869673 27.467520043538094 14.454165243505782 3 35.10406651367825 22.6410853258109 24.142784903127495 18.112063257383355 4 29.994752325944347 23.6529198018755 26.55300391775299 19.79932395442717 5 34.41538912879878 21.381484121776275 26.28963886908668 17.913487880338273 6 31.649671409276504 22.04243546880691 28.188449649866993 18.119443472049593 7 32.35007915372831 24.326598062044617 23.052003361730414 20.271319422496667 8 29.227241354386198 28.463538972404617 22.77203867974132 19.537180993467867 9 30.823893229628013 23.18627416243734 25.081683529646863 20.908149078287785 10-11 25.695968593436653 29.986564340228323 27.849203143490865 16.468263922844166 12-13 24.525538238266186 29.43720777959974 27.382966571190874 18.654287410943198 14-15 23.97779727291667 29.567595299813348 27.50310733302248 18.9515000942475 16-17 24.954176029524625 28.070191849122818 27.614949676188616 19.360682445163945 18-19 23.00743067139652 28.14776024002642 29.171231410920523 19.67357767765654 20-21 24.077359482644997 28.248569634730703 29.116355271978446 18.557715610645857 22-23 22.715435376225418 31.276698541340725 27.438287846575253 18.569578235858604 24-25 22.286019198145095 28.57044256642323 29.064540405250085 20.078997830181596 26-27 22.26084873772125 28.43292625026751 27.740058305897623 21.566166706113616 28-29 23.38181292508812 29.939539873779214 27.67100961041059 19.007637590722076 30-31 21.9261071246859 28.534302547233587 29.106973039545686 20.43261728853483 32-33 21.906988345906754 29.87866874341862 27.67011673934826 20.544226171326365 34-35 21.70680098470923 29.815615039350103 29.242320954550028 19.23526302139064 36-37 21.761788889932944 28.3836159582937 29.135591243367 20.719003908406357 38-39 22.903998948954122 29.91893652751505 27.76385312059008 19.413211402940746 40-41 21.488968621178632 31.08622921938813 27.843337625459657 19.581464533973588 42-43 21.500573226353758 29.756279943086966 29.040430847387142 19.70271598317213 44-45 21.3249820112761 28.37630667156665 29.22472816370171 21.07398315345554 46-47 22.772624678890956 28.34990521746833 27.712442200372045 21.16502790326867 48-49 21.318345638822414 29.767023443643353 29.03389579708444 19.88073512044979 50-51 22.55552524067847 28.357164370019454 29.07945844243736 20.00785194686471 52-53 21.304726854862906 28.37260910931177 27.745989074789772 22.576674961035554 54-55 22.36637627027162 28.407792057584892 29.273807741938413 19.952023930205083 56-57 21.108917908464306 28.34018817442458 29.191856406258303 21.35903751085281 58-59 21.012320379772532 28.546347717316234 29.11527504816901 21.326056854742223 60-61 22.268046994416547 28.415440892989164 29.129403033478024 20.187109079116265 62-63 20.955010317694278 28.45549218803147 29.046520329259167 21.542977165015078 64-65 22.208095025478563 28.44825264530578 29.0915173034536 20.25213502576205 66-67 20.979247716429363 30.85837644291084 27.682026761892654 20.480349078767148 68-69 20.88917819143884 31.04973638155655 27.606182367771897 20.454903059232713 70-71 20.818290328237957 30.949717929919927 27.707631040908137 20.52436070093398 72-73 20.79366938083689 30.95680879815318 27.623321411701195 20.62620040930874 74-75 20.61943214231033 30.844131173198296 27.85243015191639 20.68400653257498 76-77 20.73821046366453 30.57725478453467 27.811152482133757 20.873382269667044 78-79 20.66455489410874 30.63472103921096 27.83195177935053 20.868772287329776 80-81 20.69561862406528 30.404331992826695 27.832625848967062 21.067423534140964 82-83 20.770392492338253 30.435092558893125 27.647384461534795 21.147130487233824 84-85 20.646340104790763 30.28643262092283 27.835786983845395 21.23144029044101 86-87 20.810261568674925 30.008499614001856 27.773013243578013 21.408225573745206 88-89 20.75003990373207 30.205324901431403 27.683224754545975 21.361410440290552 90-91 20.749154651468892 29.970254562146103 27.759554568341006 21.521036218043996 92-93 20.782053343383033 29.933463264854147 27.704933512889507 21.579549878873312 94-95 20.64046652791763 29.909910471856822 27.823982564176863 21.625640436048684 96-97 20.69175671700507 29.75042972529478 27.804491825473054 21.753321732227093 98-99 20.619983083302117 29.871979216790344 27.76445242044034 21.743585279467194 100-101 20.76020154825369 29.751316375675124 27.66229511961169 21.82618695645949 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 6.0 2 10.5 3 27.5 4 26.0 5 14.5 6 17.0 7 20.5 8 22.5 9 22.5 10 30.0 11 39.5 12 50.5 13 65.0 14 85.0 15 130.0 16 209.0 17 334.5 18 547.5 19 876.5 20 1465.0 21 2381.0 22 3670.5 23 5421.5 24 7951.0 25 11259.5 26 15384.0 27 20524.5 28 26548.5 29 33619.5 30 41499.0 31 50517.0 32 60992.5 33 72609.5 34 85686.0 35 100278.5 36 116127.5 37 132538.5 38 147428.0 39 159156.5 40 168578.0 41 173968.5 42 174780.0 43 173514.5 44 170718.5 45 166966.0 46 162188.0 47 156585.0 48 148776.5 49 139142.0 50 128878.5 51 116626.0 52 102173.0 53 86651.0 54 72090.5 55 59147.5 56 48001.5 57 39149.5 58 31574.0 59 25162.5 60 20213.0 61 15820.0 62 12108.0 63 9263.0 64 6980.5 65 5212.0 66 3884.0 67 2877.5 68 2169.5 69 1621.0 70 1255.0 71 1010.5 72 767.0 73 559.0 74 431.0 75 363.0 76 282.0 77 202.5 78 145.0 79 109.5 80 93.0 81 76.5 82 54.5 83 44.0 84 34.5 85 25.5 86 21.5 87 20.5 88 12.0 89 6.0 90 4.0 91 2.5 92 2.5 93 1.5 94 1.5 95 2.0 96 1.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16377806343352858 2 0.0 3 2.5510601780923456E-4 4 0.018934535544063186 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.66902261798299E-4 12-13 0.0 14-15 0.0 16-17 0.0012046673063213853 18-19 0.0 20-21 0.0 22-23 3.826590267138518E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010345966277818957 38-39 8.64525949242406E-4 40-41 0.004747806442560755 42-43 0.0030187545440759425 44-45 0.0011479770801415556 46-47 0.003755727484413731 48-49 0.007114623385568652 50-51 0.004464355311661604 52-53 0.0014030830979507901 54-55 0.010005824920739977 56-57 0.017928284029371206 58-59 0.022137533323223574 60-61 0.010686107634897937 62-63 7.086278272478737E-4 64-65 0.013322203152260027 66-67 0.03334802555028494 68-69 0.019501437805861485 70-71 0.03544556391893865 72-73 0.028997050690982993 74-75 0.028486838655364524 76-77 0.03747223950486756 78-79 0.016340957696335968 80-81 0.011989982837034025 82-83 8.220082796075336E-4 84-85 0.009750718902930743 86-87 0.020578552103278254 88-89 0.020011649841479955 90-91 0.02407917356988275 92-93 0.04172400646835481 94-95 0.027027065331233904 96-97 0.027849073610841442 98-99 0.03548808158857352 100-101 0.015632329869088097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3527945.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.28127519919343 #Duplication Level Percentage of deduplicated Percentage of total 1 97.88329534790789 91.30678610752126 2 1.5330392337459973 2.8600770930844197 3 0.27547923466088264 0.7709116290019494 4 0.08536599389645096 0.3185219507723003 5 0.06837174523000467 0.3188901791324603 6 0.0285211483598903 0.1596293453493571 7 0.0207095632911914 0.13522701308345172 8 0.016492538435754097 0.12307560132470824 9 0.016144651319621003 0.13553942964665291 >10 0.06830054802975276 1.2030845789628333 >50 0.003235866248762762 0.2120301341152066 >100 8.407574926032688E-4 0.14165458682338974 >500 1.1014423154843703E-4 0.07842511848165637 >1k 6.296310676616094E-5 0.0826541093921426 >5k 0.0 0.0 >10k+ 3.026404288815743E-5 2.1534931233081878 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 76282 2.162221916724892 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0037699000409586883 0.0 2.834511308991495E-5 2 0.004761978999105711 0.0 2.834511308991495E-5 3 0.007114623385568653 0.0 2.834511308991495E-5 4 0.009892444468380317 0.0 2.834511308991495E-5 5 0.012783646003551643 0.0 2.834511308991495E-5 6 0.022165878436313492 0.0 2.834511308991495E-5 7 0.031066243946546786 0.0 5.66902261798299E-5 8 0.0394280523080717 0.0 5.66902261798299E-5 9 0.043821544837008515 0.0 5.66902261798299E-5 10-11 0.05361478140957413 0.0 7.086278272478737E-5 12-13 0.06594490560368713 0.0 8.503533926974485E-5 14-15 0.07743884896164765 0.0 8.503533926974485E-5 16-17 0.09077522467045263 0.0 1.2755300890461725E-4 18-19 0.10772560229822177 0.0 1.4172556544957474E-4 20-21 0.1267451731815547 0.0 1.4172556544957474E-4 22-23 0.1491378125225875 0.0 1.5589812199453222E-4 24-25 0.17114779283690645 0.0 1.842432350844472E-4 26-27 0.19198145095799396 0.0 2.125883481743621E-4 28-29 0.21521027113517927 0.0 2.69278574354192E-4 30-31 0.24491594965341013 0.0 3.1179624398906445E-4 32-33 0.27849073610841435 0.0 3.401413570789794E-4 34-35 0.31800382375575587 0.0 3.401413570789794E-4 36-37 0.36478743291066046 0.0 3.826590267138518E-4 38-39 0.4188132184600384 0.0 3.968315832588093E-4 40-41 0.47689235518127404 0.0 4.2517669634872427E-4 42-43 0.5432057472551302 0.0 4.535218094386392E-4 44-45 0.6150747814946095 0.0 4.6769436598359667E-4 46-47 0.6929529797091507 0.0 4.8186692252855416E-4 48-49 0.7746719407473756 0.0 4.8186692252855416E-4 50-51 0.8616489202637796 0.0 4.960394790735116E-4 52-53 0.952495007716957 0.0 5.102120356184691E-4 54-55 1.0496478828326405 0.0 5.385571487083841E-4 56-57 1.1523422275574025 0.0 5.385571487083841E-4 58-59 1.2581970523916897 0.0 5.527297052533415E-4 60-61 1.3643778460265112 0.0 5.66902261798299E-4 62-63 1.4748529242944548 0.0 5.66902261798299E-4 64-65 1.5906143661536674 0.0 5.952473748882139E-4 66-67 1.7113220302470702 0.0 5.952473748882139E-4 68-69 1.8381805838809846 0.0 5.952473748882139E-4 70-71 1.9652942435327083 0.0 6.235924879781289E-4 72-73 2.0983178592636786 0.0 6.235924879781289E-4 74-75 2.2376766077702457 0.0 6.235924879781289E-4 76-77 2.3833704890524086 0.0 6.235924879781289E-4 78-79 2.533401172637329 0.0 6.661101576130013E-4 80-81 2.6868899600192178 0.0 6.944552707029163E-4 82-83 2.8489389715542615 0.0 7.511454968827461E-4 84-85 3.020256835069708 0.0 7.653180534277036E-4 86-87 3.203777269770362 0.0 7.653180534277036E-4 88-89 3.3942989474042253 0.0 7.653180534277036E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCGC 25 0.004992851 57.08428 1 CGGGTCG 55 4.922304E-5 43.245667 1 CGGAAGA 23430 0.0 37.400864 4 TCGGAAG 23640 0.0 37.10881 3 GAGCACA 23575 0.0 36.883022 9 CGACGGC 65 1.3172421E-4 36.592487 1 CGTCTTC 11810 0.0 36.584454 52-53 AGAGCAC 23895 0.0 36.50833 8 GCCGTCT 11950 0.0 36.352043 50-51 ATCGGAA 24265 0.0 36.17256 2 GATCGGA 24565 0.0 35.8447 1 GAAGAGC 24565 0.0 35.7059 6 CGACGGG 80 1.0673761E-5 35.677677 1 CCGTCTT 12040 0.0 35.66857 52-53 TGCCGTC 12065 0.0 35.651196 50-51 ATGCCGT 12220 0.0 35.406517 48-49 TATGCCG 12280 0.0 34.88544 48-49 CGTATGC 12325 0.0 34.545883 46-47 TGCTTGA 12315 0.0 34.390617 60-61 CTGCTTG 12605 0.0 34.35171 58-59 >>END_MODULE