FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34211-2.34100000035863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34211-2.34100000035863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527945
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT762822.162221916724892TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC250.00499285157.084281
CGGGTCG554.922304E-543.2456671
CGGAAGA234300.037.4008644
TCGGAAG236400.037.108813
GAGCACA235750.036.8830229
CGACGGC651.3172421E-436.5924871
CGTCTTC118100.036.58445452-53
AGAGCAC238950.036.508338
GCCGTCT119500.036.35204350-51
ATCGGAA242650.036.172562
GATCGGA245650.035.84471
GAAGAGC245650.035.70596
CGACGGG801.0673761E-535.6776771
CCGTCTT120400.035.6685752-53
TGCCGTC120650.035.65119650-51
ATGCCGT122200.035.40651748-49
TATGCCG122800.034.8854448-49
CGTATGC123250.034.54588346-47
TGCTTGA123150.034.39061760-61
CTGCTTG126050.034.3517158-59
Produced by FastQC (version 0.11.2)