FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34211-1.3410000003589a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34211-1.3410000003589a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3843546
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT352820.9179544098080262TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC405.390222E-447.4991872
TCGGAAG95050.041.7273253
CGGGTCG802.2353015E-741.6311261
CGGAAGA95850.041.4781654
ATCGGAA98100.040.526832
GATCGGA100550.039.6526371
GCCGTCT51350.038.06583450-51
TATGCCG50650.038.02451348-49
TGCCGTC51150.037.8896450-51
ATGCCGT51450.037.7102148-49
CGTATGC51000.037.669746-47
CGACGGG1152.4665496E-937.2352941
CGTCTTC52900.037.0863852-53
CTCGTAT51100.036.8991444-45
TCGTATG52750.036.73536344-45
TCTCGTA51550.036.48489842-43
GAGCACA109100.036.2576039
AGAGCAC109500.036.2118878
CCGTCTT53650.036.16949552-53
GAAGAGC111250.035.599586
Produced by FastQC (version 0.11.2)