##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34139-1.341000000356ec.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2792336 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.676046865420204 33.0 33.0 33.0 33.0 33.0 2 32.705692294910065 33.0 33.0 33.0 33.0 33.0 3 32.73920366316948 33.0 33.0 33.0 33.0 33.0 4 36.65042924633712 37.0 37.0 37.0 37.0 37.0 5 36.660405123165695 37.0 37.0 37.0 37.0 37.0 6 36.5587952166215 37.0 37.0 37.0 37.0 37.0 7 36.44168753330545 37.0 37.0 37.0 37.0 37.0 8 36.40778795961518 37.0 37.0 37.0 37.0 37.0 9 36.45266293168157 37.0 37.0 37.0 37.0 37.0 10-11 36.37599701468591 37.0 37.0 37.0 37.0 37.0 12-13 36.366032955919344 37.0 37.0 37.0 37.0 37.0 14-15 38.25744770686622 40.0 37.0 40.0 37.0 40.0 16-17 38.27435237020187 40.0 37.0 40.0 37.0 40.0 18-19 38.23265591963145 40.0 37.0 40.0 37.0 40.0 20-21 38.232057675007596 40.0 37.0 40.0 37.0 40.0 22-23 38.028921483660994 40.0 37.0 40.0 33.0 40.0 24-25 38.11266427106193 40.0 37.0 40.0 37.0 40.0 26-27 38.04885801708677 40.0 37.0 40.0 37.0 40.0 28-29 37.87058559571628 40.0 37.0 40.0 33.0 40.0 30-31 37.786338929126 40.0 37.0 40.0 33.0 40.0 32-33 37.714315719884716 40.0 37.0 40.0 33.0 40.0 34-35 37.60067251935297 40.0 37.0 40.0 33.0 40.0 36-37 37.56254351195558 40.0 37.0 40.0 33.0 40.0 38-39 37.513349038224625 40.0 37.0 40.0 33.0 40.0 40-41 37.45047712739441 40.0 37.0 40.0 33.0 40.0 42-43 37.02644273468522 38.5 37.0 40.0 33.0 40.0 44-45 36.90292446897508 37.0 37.0 40.0 33.0 40.0 46-47 37.00478058514448 37.0 37.0 40.0 33.0 40.0 48-49 36.87029533695086 37.0 37.0 40.0 33.0 40.0 50-51 36.79891334710436 37.0 37.0 40.0 33.0 40.0 52-53 36.703730675678 37.0 37.0 40.0 33.0 40.0 54-55 36.45903107648936 37.0 37.0 40.0 33.0 40.0 56-57 36.30193841285576 37.0 37.0 40.0 33.0 40.0 58-59 36.08508915116232 37.0 37.0 40.0 33.0 40.0 60-61 35.83541522223686 37.0 37.0 40.0 30.0 40.0 62-63 35.50206099838988 37.0 35.0 40.0 27.0 40.0 64-65 35.22387653205058 37.0 33.0 37.0 27.0 40.0 66-67 34.7094918018462 37.0 33.0 37.0 27.0 40.0 68-69 34.452759087731565 37.0 33.0 37.0 27.0 40.0 70-71 34.028138089398986 37.0 33.0 37.0 27.0 40.0 72-73 33.18129891961426 37.0 33.0 37.0 24.5 37.0 74-75 32.41498784530228 37.0 33.0 37.0 22.0 37.0 76-77 29.7625298316535 33.0 27.0 35.0 18.5 37.0 78-79 31.967666677649106 35.0 33.0 37.0 22.0 37.0 80-81 32.2441006383186 37.0 33.0 37.0 18.5 37.0 82-83 32.102421771591956 37.0 33.0 37.0 15.0 37.0 84-85 31.94483543527713 37.0 33.0 37.0 15.0 37.0 86-87 31.8338647283135 37.0 33.0 37.0 15.0 37.0 88-89 31.561984839933302 33.0 33.0 37.0 15.0 37.0 90-91 31.53979696569467 33.0 33.0 37.0 15.0 37.0 92-93 31.39497861288899 33.0 33.0 37.0 15.0 37.0 94-95 31.220787183204315 33.0 33.0 37.0 15.0 37.0 96-97 31.106386015150036 33.0 33.0 37.0 15.0 37.0 98-99 30.983412096538526 33.0 33.0 37.0 15.0 37.0 100-101 29.443780762773535 33.0 30.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1518205157622532 2306 2 0.150677052932906 2306 3 0.09280882789237666 2306 4 0.18976311602204277 2306 5 0.16398184695331253 2306 6 0.12436166604442178 2306 7 0.13779083466125286 2306 8 0.22436990473183727 2306 9 0.1785475670858716 2306 10-11 0.15287742827032247 2306 12-13 0.2103793959636988 2306 14-15 0.2769724100283355 2306 16-17 0.31301323928692426 2306 18-19 0.3367259248562675 2306 20-21 0.2689903316537894 2306 22-23 0.3100233641680603 2306 24-25 0.2978866504320834 2306 26-27 0.18072649139561037 2306 28-29 0.19710834993458803 2306 30-31 0.3014076277510256 2306 32-33 0.40272525932144276 2306 34-35 0.4366056567326595 2306 36-37 0.23703729809396634 2306 38-39 0.3911422578283492 2306 40-41 0.3889660237813857 2306 42-43 0.4518938568606785 2306 44-45 0.3978361483833055 2306 46-47 0.42381380936127044 2306 48-49 0.5190620776258683 2306 50-51 0.5197807885926764 2306 52-53 0.41029236024400717 2306 54-55 0.45331872681673957 2306 56-57 0.37732928484052053 2306 58-59 0.4726999449233915 2306 60-61 0.42660834610417453 2306 62-63 0.3881680524302453 2306 64-65 0.3792007235741508 2306 66-67 0.39402452733673243 2306 68-69 0.3636586204435375 2306 70-71 0.16706675231943535 2306 72-73 -0.02983497039598859 2306 74-75 0.10595141756582649 2306 76-77 -0.29371514233943685 2306 78-79 0.1947074872536163 2306 80-81 0.3829319013948762 2306 82-83 0.6168784506401366 2306 84-85 0.3747180807831825 2306 86-87 0.525950869409229 2306 88-89 0.7320201406882205 2306 90-91 0.6401925910390958 2306 92-93 0.46356991356522315 2306 94-95 0.5805039777812411 2306 96-97 0.5557090981799355 2306 98-99 0.46342195764659877 2306 100-101 0.4011697961700662 2307 1 0.14351724244353647 2307 2 0.09883244850076522 2307 3 0.10839146084624218 2307 4 0.12827145839724352 2307 5 0.17233148698098688 2307 6 0.14644397143992904 2307 7 0.15712553862584855 2307 8 0.1355859902619585 2307 9 0.1993809627400509 2307 10-11 0.20686248665177942 2307 12-13 0.2216650956587003 2307 14-15 0.4027656119423213 2307 16-17 0.42904742415803554 2307 18-19 0.3553368167167079 2307 20-21 0.3124088016720492 2307 22-23 0.32521241427444636 2307 24-25 0.3783938936812419 2307 26-27 0.41791261334817165 2307 28-29 0.36236656549576907 2307 30-31 0.2463532504986503 2307 32-33 0.3630551210631836 2307 34-35 0.3350871363306709 2307 36-37 0.37724637990065446 2307 38-39 0.42668447696041056 2307 40-41 0.33510339915216747 2307 42-43 0.4601268322658001 2307 44-45 0.5497761143924507 2307 46-47 0.588137960091963 2307 48-49 0.4379497167740709 2307 50-51 0.5425729106110495 2307 52-53 0.40123568132257503 2307 54-55 0.13973330245879367 2307 56-57 0.20819902173828808 2307 58-59 0.3586508748430077 2307 60-61 0.5053701071160575 2307 62-63 0.5168004139999738 2307 64-65 0.5000521317332343 2307 66-67 0.4387429745776217 2307 68-69 0.3281722764008421 2307 70-71 0.34145012850814993 2307 72-73 0.2799737038964736 2307 74-75 0.21827359482683306 2307 76-77 -0.12459641749658346 2307 78-79 0.22555459634273944 2307 80-81 0.41875638053861763 2307 82-83 0.42305000917266256 2307 84-85 0.42593043133601327 2307 86-87 0.3775114830907853 2307 88-89 0.6734714870535186 2307 90-91 0.3613423486715419 2307 92-93 0.523069298405062 2307 94-95 0.5381329253082292 2307 96-97 0.5077535281706922 2307 98-99 0.39326463989020155 2307 100-101 0.5074573569435756 2201 1 0.10935863308935723 2201 2 0.12327907755491907 2201 3 0.11647064901232795 2201 4 0.19132500083335202 2201 5 0.1720921172788792 2201 6 0.18754839744509866 2201 7 0.22925690570122725 2201 8 0.2674846342909589 2201 9 0.24493123559898322 2201 10-11 0.18127071212369827 2201 12-13 0.21546380273468912 2201 14-15 0.3816129270394626 2201 16-17 0.3657847147837785 2201 18-19 0.32619033044483814 2201 20-21 0.34407757707180764 2201 22-23 0.25331478587737166 2201 24-25 0.4361955338039465 2201 26-27 0.42605865712395996 2201 28-29 0.4470426249831334 2201 30-31 0.3842395156944818 2201 32-33 0.48402681462327735 2201 34-35 0.4722281611237591 2201 36-37 0.36371349400505437 2201 38-39 0.5373536493985327 2201 40-41 0.5100390365502676 2201 42-43 0.42711418744355 2201 44-45 0.4758015596579739 2201 46-47 0.5610214067044339 2201 48-49 0.4681694761798738 2201 50-51 0.5001681276399239 2201 52-53 0.4447248970929607 2201 54-55 0.4911652922844496 2201 56-57 0.5944039144222373 2201 58-59 0.6525255085960993 2201 60-61 0.36643206905893777 2201 62-63 0.5740478589997196 2201 64-65 0.525135168857652 2201 66-67 0.42418903049593837 2201 68-69 0.5156593634394753 2201 70-71 0.25437873388374754 2201 72-73 0.10650080171391352 2201 74-75 0.12790334170309592 2201 76-77 0.043372619653634814 2201 78-79 0.18025219091270372 2201 80-81 0.30305512047929994 2201 82-83 0.4748423230913872 2201 84-85 0.35898308714183713 2201 86-87 0.3493498116882563 2201 88-89 0.6175883879264106 2201 90-91 0.30773817606244336 2201 92-93 0.5029763721790523 2201 94-95 0.5141196803212225 2201 96-97 0.2691397142250409 2201 98-99 0.45944575532024956 2201 100-101 0.38077713623201603 1101 1 0.13664464969639312 1101 2 0.10194438832429142 1101 3 0.09808492554664383 1101 4 0.12948226398027884 1101 5 0.14779996329043144 1101 6 0.14316443955280533 1101 7 0.15129823076073023 1101 8 0.11995191039699904 1101 9 0.05753047519778676 1101 10-11 0.06818551489348579 1101 12-13 0.0983000127307605 1101 14-15 0.167792123500341 1101 16-17 0.2183982289953903 1101 18-19 0.20377813520301657 1101 20-21 0.22163886866744775 1101 22-23 0.19850810655583473 1101 24-25 0.27628522774043063 1101 26-27 0.23516712016238728 1101 28-29 0.1846216240456613 1101 30-31 0.19941579773984586 1101 32-33 0.22757755712115824 1101 34-35 0.26409928091780444 1101 36-37 0.28405483572997525 1101 38-39 0.32436528525881414 1101 40-41 0.3903746026552497 1101 42-43 0.4222059266811442 1101 44-45 0.37993624578462715 1101 46-47 0.4193337727760138 1101 48-49 0.40542122706478523 1101 50-51 0.4384832688174356 1101 52-53 0.3445412634187619 1101 54-55 0.3502661247053709 1101 56-57 0.39531556073997365 1101 58-59 0.37689402418786244 1101 60-61 0.4327140516005201 1101 62-63 0.460735313710245 1101 64-65 0.35972296585588737 1101 66-67 0.400772827055178 1101 68-69 0.24427201740520132 1101 70-71 0.0706267204538591 1101 72-73 -0.20762862612280486 1101 74-75 -0.31049706654417975 1101 76-77 -0.41057937507846987 1101 78-79 -0.005395929350257234 1101 80-81 0.068599408915901 1101 82-83 0.11714798160997475 1101 84-85 0.13254913152209724 1101 86-87 0.08144899271791317 1101 88-89 0.2672961149520674 1101 90-91 0.11804998563474456 1101 92-93 0.22730442517433858 1101 94-95 0.21912425684412185 1101 96-97 0.26299595856443503 1101 98-99 0.014580015388691692 1101 100-101 -0.05355613440584861 2304 1 0.05352877963755276 2304 2 0.06760775087299464 2304 3 0.053923471103729526 2304 4 0.060169920086366346 2304 5 0.0994665235007588 2304 6 0.10596109283024191 2304 7 0.1962700220370266 2304 8 0.03134686463125291 2304 9 0.05122897694339912 2304 10-11 0.031213338388653256 2304 12-13 0.08867972429254678 2304 14-15 0.2594508666161133 2304 16-17 0.24478398440901827 2304 18-19 0.1418750698230511 2304 20-21 0.21800434994000994 2304 22-23 0.2340662891376155 2304 24-25 0.13166326964760344 2304 26-27 0.16506421383812153 2304 28-29 0.2028256581562573 2304 30-31 0.30521778960967083 2304 32-33 0.273189989412451 2304 34-35 0.21875798134072255 2304 36-37 0.24582139981244922 2304 38-39 0.2461935951215537 2304 40-41 0.21401017712069148 2304 42-43 0.3999992720898291 2304 44-45 0.3073582295593553 2304 46-47 0.30591143752987904 2304 48-49 0.26832884866578155 2304 50-51 0.2872563291636183 2304 52-53 0.25909904654105986 2304 54-55 0.30110968915487035 2304 56-57 0.03727111847246789 2304 58-59 0.22435370012594547 2304 60-61 0.27974671626669334 2304 62-63 0.25582426211907716 2304 64-65 0.23426670666989224 2304 66-67 0.30165044650167516 2304 68-69 0.3485186159596694 2304 70-71 0.2855403104495693 2304 72-73 0.10164171329861205 2304 74-75 0.09488613821968528 2304 76-77 -0.02616440120550223 2304 78-79 0.14117426249951137 2304 80-81 0.1839385374455489 2304 82-83 0.23818155904273652 2304 84-85 0.25483279872567266 2304 86-87 0.11755407406103302 2304 88-89 0.3593606056511476 2304 90-91 0.13341271106707708 2304 92-93 0.12332689088188431 2304 94-95 0.09907410432473185 2304 96-97 0.15049673216555703 2304 98-99 0.14873022738022001 2304 100-101 0.2521447184252672 2202 1 0.10815462977834756 2202 2 0.1396803509192921 2202 3 0.052834803533208685 2202 4 0.07582643721688243 2202 5 0.06312516830627146 2202 6 0.02633937974959366 2202 7 0.12868750098891013 2202 8 0.08758343075258068 2202 9 0.010091870683183402 2202 10-11 0.09245755942071554 2202 12-13 0.006275115337651016 2202 14-15 0.027938590304607658 2202 16-17 0.021874479842985295 2202 18-19 -0.03132038926570857 2202 20-21 0.0859700560633101 2202 22-23 0.13807400157391214 2202 24-25 0.0922914118662348 2202 26-27 -0.0024543350739634207 2202 28-29 0.18712634385180849 2202 30-31 0.24454620247609427 2202 32-33 0.17852965785849761 2202 34-35 0.10962487935375265 2202 36-37 0.14505610923043122 2202 38-39 0.1811876181684866 2202 40-41 0.2604871368958186 2202 42-43 0.17762677566666696 2202 44-45 0.07021577295838455 2202 46-47 0.26555439651569657 2202 48-49 0.20530603877453046 2202 50-51 0.20177109480552247 2202 52-53 0.213440630912487 2202 54-55 0.20674878359443483 2202 56-57 0.2376702348920361 2202 58-59 0.311330049713618 2202 60-61 0.2536285649133774 2202 62-63 0.27656192192025486 2202 64-65 0.1951433809454599 2202 66-67 0.18618166630749755 2202 68-69 0.3248834718149354 2202 70-71 0.012039919568714197 2202 72-73 -0.16397805995192982 2202 74-75 0.13145034716674076 2202 76-77 -0.024712458884678767 2202 78-79 0.15561842437121953 2202 80-81 0.24596475152426223 2202 82-83 0.29766484986934927 2202 84-85 0.23755947376465514 2202 86-87 0.35826558363226013 2202 88-89 0.4515798018261421 2202 90-91 0.302878338613219 2202 92-93 0.4067428086237115 2202 94-95 0.2545274744518089 2202 96-97 0.39524752383616146 2202 98-99 0.3899088815995704 2202 100-101 0.43901838564029916 1102 1 0.13480142786252713 1102 2 0.06717715292489856 1102 3 0.08844210647561823 1102 4 0.10245017643418919 1102 5 0.06248165511425441 1102 6 0.044228220923393735 1102 7 0.09650159300261407 1102 8 0.046590031993744674 1102 9 0.13840208133446907 1102 10-11 0.08096420884351829 1102 12-13 0.03855589205707588 1102 14-15 0.10226863414349197 1102 16-17 0.1338680512586521 1102 18-19 0.1454075347516266 1102 20-21 0.13244840485486264 1102 22-23 0.11003712389820919 1102 24-25 0.10264171163862557 1102 26-27 0.17894977057152062 1102 28-29 0.19831345274958068 1102 30-31 0.19686729805125225 1102 32-33 0.19497525594216825 1102 34-35 0.02646691914111443 1102 36-37 0.030143811922499708 1102 38-39 0.18081291158189572 1102 40-41 0.017918177005860514 1102 42-43 0.08406940883554626 1102 44-45 -0.08620888995158538 1102 46-47 0.09897543420171218 1102 48-49 0.05181326808514086 1102 50-51 0.10196363634386074 1102 52-53 -0.05972459853676071 1102 54-55 0.11084987423613768 1102 56-57 0.041283488160807735 1102 58-59 0.29034071614761103 1102 60-61 0.17596160412753648 1102 62-63 0.08225424518222724 1102 64-65 0.12335821044435136 1102 66-67 0.048628266170823053 1102 68-69 0.17544977338761925 1102 70-71 0.030896806134883548 1102 72-73 -0.039511491963928336 1102 74-75 -0.03665484169035693 1102 76-77 0.06853375558242547 1102 78-79 0.14589961017203024 1102 80-81 0.29071909417076824 1102 82-83 0.1362578348211585 1102 84-85 0.10763478658594039 1102 86-87 0.20276747438816756 1102 88-89 0.4116077542969805 1102 90-91 0.1420692172087108 1102 92-93 0.27097476260504294 1102 94-95 0.3947860924542077 1102 96-97 0.21735334780847282 1102 98-99 0.14374131509146082 1102 100-101 0.2028734552531688 2305 1 0.12001008234872756 2305 2 0.06173550443320863 2305 3 0.04234273692033952 2305 4 -0.016062403501962308 2305 5 0.011151014506353363 2305 6 -0.009222590557001809 2305 7 -0.08397712608307017 2305 8 -0.018020436525141292 2305 9 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-1.0599148171498527 1213 100-101 -0.8246776541691894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 8.0 10 18.0 11 15.0 12 23.0 13 81.0 14 307.0 15 786.0 16 1580.0 17 2466.0 18 3105.0 19 3766.0 20 5327.0 21 6822.0 22 9182.0 23 12078.0 24 16234.0 25 21414.0 26 28695.0 27 41371.0 28 67647.0 29 106451.0 30 89089.0 31 62152.0 32 76291.0 33 106712.0 34 162965.0 35 283623.0 36 555518.0 37 880252.0 38 247807.0 39 548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97410018888268 24.620530894477497 18.39558044658953 15.009788470050294 2 29.58243563811805 28.419789022524512 27.640119240664447 14.357656098692996 3 35.892846399133624 18.55000558673623 28.59044496205317 16.966703052076976 4 29.954999407179965 18.54584648074445 26.95815980064053 24.540994311435053 5 38.061895130098954 16.666081732284365 27.5009526074226 17.771070530194073 6 34.83470470602391 17.473577678330972 29.772097627219647 17.91961998842546 7 32.150966072850835 24.92801725866801 23.146605566092333 19.774411102388825 8 28.82872978037027 31.24738570143421 20.96248445745784 18.961400060737677 9 34.478049919493934 22.23024020031973 23.503618475713527 19.788091404472812 10-11 26.287965049869793 30.02544656838405 27.343520436310794 16.343067945435365 12-13 22.581773826645506 29.192475404106094 26.979131451229364 21.246619318019036 14-15 22.486710052085424 29.470629609044185 27.10945244411847 20.93320789475192 16-17 25.799276368966595 25.662865866826245 27.468451713941196 21.06940605026596 18-19 21.22792529265819 25.709334406747615 31.404744987709215 21.65799531288498 20-21 24.616217389311316 25.839565868863918 31.25261429856579 18.291602443258977 22-23 20.608260250554693 33.355936962197624 27.49119953644356 18.544603250804123 24-25 20.717546169228918 25.264724589017938 31.604738827992048 22.412990413761094 26-27 20.768381742025316 25.416908996625047 27.816279989227656 25.99842927212198 28-29 24.085031314283096 29.966433122661456 27.355178603148044 18.593356959907403 30-31 19.776864245563573 27.140161499189208 30.883461016152786 22.199513239094433 32-33 19.470794345666135 31.192986803880334 27.408091289873425 21.9281275605801 34-35 19.711775373737257 30.9562853467491 31.48845626027813 17.843483019235507 36-37 19.56924942484937 31.10775228556121 31.713898733379363 17.60909955621005 38-39 19.837679342838594 27.172334758376792 31.215506860353205 21.77447903843141 40-41 23.61997482634241 26.610318119607644 27.37663018593373 22.393076868116214 42-43 20.0395917625497 30.289542742897968 30.915454306969565 18.755411187582762 44-45 19.567425710608774 26.581107768792574 31.44907265731753 22.402393863281127 46-47 23.35719185802999 26.43780127199703 27.761137315834496 22.443869554138484 48-49 19.538659180901842 30.884975846517943 30.88107203639927 18.695292936180945 50-51 23.34132104102597 26.981935794874808 30.393412809293867 19.283330354805354 52-53 19.56117872068477 26.840361086037184 26.829885844941003 26.768574348337044 54-55 23.632909034906127 26.601953396416985 30.80039469065851 18.96474287801838 56-57 19.942167027898876 26.59591386734092 30.42653339221938 23.03538571254083 58-59 19.84005920363446 27.081740453602 30.51519663232763 22.56300371043591 60-61 23.15239336525991 27.058868615713795 30.635118306351185 19.15361971267511 62-63 19.4592449713822 27.139262075451292 30.30768701845564 23.093805934710876 64-65 23.317963635295634 26.999402392328985 30.41203410285693 19.270599869518446 66-67 20.024281757228284 34.058120023099555 26.65107351303792 19.266524706634243 68-69 20.25405845013528 34.068791139475145 26.643111958115888 19.034038452273684 70-71 19.756997995582758 34.52632879966038 26.684543417397048 19.03212978735981 72-73 19.69514843602371 34.375345353655575 26.483075096116902 19.446431114203808 74-75 20.08617459454309 33.415151912455556 26.559568152655416 19.939105340345943 76-77 20.415232289404038 32.37533232949316 26.659085917899343 20.550349463203453 78-79 20.100502062586433 32.06853582331352 27.15593740855186 20.675024705548193 80-81 19.6363036597965 31.749325170008092 27.48497985046201 21.129391319733404 82-83 19.383965357720783 31.56094209436952 27.351542745693934 21.703549802215765 84-85 19.37870468863603 31.324536992267387 27.82784815566945 21.468910163427136 86-87 19.560577209632864 31.109641317928087 28.338287169171167 20.991494303267885 88-89 19.846520802685312 31.43732007440417 28.026663285310192 20.689495837600326 90-91 20.32604227767504 30.858529639596476 27.485772366824428 21.329655715904053 92-93 19.976586690599476 31.134291541382176 27.282363257405777 21.60675851061257 94-95 19.642820041625022 31.15375108808958 27.49517300176602 21.708255868519373 96-97 19.77867528647569 31.619604775762035 27.192788104262338 21.408931833499935 98-99 19.98262115973218 31.373249017357523 27.03857002703554 21.605559795874758 100-101 20.072803545403 31.187146230681144 26.848393762377338 21.891656461538517 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 70.0 1 43.5 2 28.5 3 50.0 4 50.5 5 36.5 6 34.5 7 43.5 8 54.0 9 54.5 10 59.0 11 65.5 12 60.5 13 65.0 14 84.0 15 107.0 16 126.5 17 135.5 18 171.5 19 240.5 20 307.5 21 411.5 22 647.5 23 972.0 24 1429.0 25 2164.5 26 3107.0 27 4335.0 28 5924.0 29 7794.5 30 10095.0 31 13544.5 32 19394.0 33 29117.5 34 43742.5 35 64764.0 36 94998.0 37 133589.0 38 170424.5 39 193458.0 40 203895.0 41 199123.5 42 185865.0 43 178231.5 44 165610.5 45 151759.5 46 146694.5 47 135673.0 48 114260.5 49 94205.0 50 77566.0 51 65244.0 52 56109.0 53 46795.5 54 38439.0 55 30812.5 56 24238.5 57 18674.0 58 14060.5 59 10563.5 60 8080.0 61 6260.5 62 4881.0 63 3586.5 64 2527.0 65 1886.5 66 1401.5 67 1017.5 68 792.5 69 611.5 70 424.5 71 306.0 72 217.5 73 160.5 74 137.5 75 118.0 76 82.5 77 60.0 78 47.5 79 37.0 80 27.5 81 19.5 82 15.5 83 13.5 84 7.5 85 7.5 86 8.0 87 5.0 88 3.5 89 2.5 90 2.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1562132923831516 2 0.0 3 2.8649847296313913E-4 4 0.02130832392663347 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.192784822456896E-4 12-13 0.0 14-15 0.0 16-17 0.0010385569644913793 18-19 0.0 20-21 0.0 22-23 4.118415548845125E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.594954188894173E-4 38-39 8.415892643292211E-4 40-41 0.0047093186493315994 42-43 0.0031693893571547263 44-45 0.0010564631190515756 46-47 0.004028884776044144 48-49 0.006875963351115339 50-51 0.004619787876530618 52-53 0.0013429615920147145 54-55 0.009884197317228298 56-57 0.018013591487557372 58-59 0.022042476263601514 60-61 0.010922754281719679 62-63 6.44621564167063E-4 64-65 0.013268460529105379 66-67 0.03366357057316884 68-69 0.01924911615221091 70-71 0.03584812142951278 72-73 0.02913331346943921 74-75 0.028434973441591554 76-77 0.03778198612201397 78-79 0.01622297603153775 80-81 0.01205084201901204 82-83 9.132138825700059E-4 84-85 0.009526074226024375 86-87 0.020054893107419737 88-89 0.01994745618005856 90-91 0.02411959019258427 92-93 0.042562929389586356 94-95 0.02671598260381272 96-97 0.027987319577586652 98-99 0.03618833836615651 100-101 0.01572160370385226 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2792336.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.575075505609256 #Duplication Level Percentage of deduplicated Percentage of total 1 90.74075381879295 47.70701983458941 2 4.423332196632495 4.651140484486918 3 1.3543975290868016 2.1362265706904755 4 0.6953722456383387 1.4623699327576287 5 0.4998641180734116 1.3140196875127197 6 0.345426424081706 1.0896492196636975 7 0.2554639006045809 0.940172370427031 8 0.20588789606815344 0.8659657345179359 9 0.16269295238656958 0.7698234830358949 >10 1.0709875509657532 11.214542123848265 >50 0.1194592408446268 4.41661793824911 >100 0.11632516564197101 12.324293551490715 >500 0.007766485893205906 2.7193543888883465 >1k 0.0021347033282825396 1.6021052883131408 >5k 6.788598091231357E-5 0.19894222329650343 >10k+ 6.788598036938922E-5 6.587757168232129 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 184577 6.610128580514665 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 5574 0.19961781103706716 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004261664785326694 0.0 0.0 2 0.0047272248038917955 0.0 0.0 3 0.00540765867717925 0.0 0.0 4 0.005909031004864744 0.0 0.0 5 0.007448960297041617 0.0 0.0 6 0.009418637298663199 0.0 0.0 7 0.011316689682043996 0.0 0.0 8 0.013429615920147145 0.0 0.0 9 0.015578354467370688 0.0 3.581230912039239E-5 10-11 0.025319302548117418 0.0 3.581230912039239E-5 12-13 0.033735195191409634 0.0 3.581230912039239E-5 14-15 0.04275989708974851 0.0 3.581230912039239E-5 16-17 0.05189203591544857 0.0 3.581230912039239E-5 18-19 0.06079139473186608 0.0 3.581230912039239E-5 20-21 0.06795385655594456 0.0 3.581230912039239E-5 22-23 0.07542072300754638 0.0 3.581230912039239E-5 24-25 0.08312036946843074 0.0 3.581230912039239E-5 26-27 0.09162579288452392 0.0 3.581230912039239E-5 28-29 0.100668400937423 0.0 3.581230912039239E-5 30-31 0.2003519633740352 0.0 3.581230912039239E-5 32-33 0.2149991978042757 0.0 3.581230912039239E-5 34-35 0.22626216902263913 0.0 3.581230912039239E-5 36-37 0.2375072340864423 0.0 3.581230912039239E-5 38-39 0.2509189438520293 0.0 8.953077280098097E-5 40-41 0.26644357985571937 0.0 1.0743692736117717E-4 42-43 0.2864089421903381 0.0 1.0743692736117717E-4 44-45 0.3075023922622493 0.0 1.0743692736117717E-4 46-47 0.32895396542536426 0.0 1.0743692736117717E-4 48-49 0.35400467565507876 0.0 1.0743692736117717E-4 50-51 0.3827440537241936 0.0 1.0743692736117717E-4 52-53 0.41492141346886624 0.0 1.0743692736117717E-4 54-55 0.44908635636972055 0.0 1.2534308192137337E-4 56-57 0.48819339792918903 0.0 1.4324923648156957E-4 58-59 0.5314904796557434 0.0 1.4324923648156957E-4 60-61 0.5747338429186173 0.0 1.4324923648156957E-4 62-63 0.6242264541229996 0.0 1.9696770016215814E-4 64-65 0.675742460792684 0.0 2.1487385472235434E-4 66-67 0.7302129829648007 0.0 2.1487385472235434E-4 68-69 0.7880677683487947 0.0 2.1487385472235434E-4 70-71 0.8508646523914027 0.0 2.1487385472235434E-4 72-73 0.9217551182952195 0.0 2.1487385472235434E-4 74-75 0.9941317950275326 0.0 2.5068616384274673E-4 76-77 1.0734560597292018 0.0 2.5068616384274673E-4 78-79 1.1560571507153867 0.0 2.5068616384274673E-4 80-81 1.2469308851083825 0.0 2.5068616384274673E-4 82-83 1.3460414505990683 0.0 2.5068616384274673E-4 84-85 1.4547855272431398 0.0 2.5068616384274673E-4 86-87 1.5717485288303412 0.0 2.685923184029429E-4 88-89 1.6952651829865748 0.0 2.8649847296313913E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 26925 0.0 74.223564 3 CGGAAGA 27005 0.0 73.93333 4 GATCGGA 27150 0.0 73.62744 1 GAAGAGC 27245 0.0 73.29985 6 ATCGGAA 27425 0.0 72.905 2 GGAAGAG 29515 0.0 67.807175 5 GAGCACA 34220 0.0 58.289886 9 AGAGCAC 34265 0.0 58.213337 8 AAGAGCA 34730 0.0 57.379223 7 ACGACGG 50 4.1929707E-7 57.10026 1 CGACGGG 95 7.2759576E-12 50.08795 1 GCCGTCT 22260 0.0 44.983677 50-51 CGTCTTC 22220 0.0 44.905926 52-53 ATGCCGT 22265 0.0 44.787415 48-49 TCGTATG 22365 0.0 44.533268 44-45 TGCCGTC 22280 0.0 44.50623 50-51 TATGCCG 22230 0.0 44.473343 48-49 TCTCGTA 21720 0.0 44.29222 42-43 CCGTCTT 22350 0.0 44.2409 52-53 CGTATGC 22300 0.0 44.237103 46-47 >>END_MODULE