FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34139-1.341000000356ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34139-1.341000000356ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792336
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1845776.610128580514665TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT55740.19961781103706716TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG269250.074.2235643
CGGAAGA270050.073.933334
GATCGGA271500.073.627441
GAAGAGC272450.073.299856
ATCGGAA274250.072.9052
GGAAGAG295150.067.8071755
GAGCACA342200.058.2898869
AGAGCAC342650.058.2133378
AAGAGCA347300.057.3792237
ACGACGG504.1929707E-757.100261
CGACGGG957.2759576E-1250.087951
GCCGTCT222600.044.98367750-51
CGTCTTC222200.044.90592652-53
ATGCCGT222650.044.78741548-49
TCGTATG223650.044.53326844-45
TGCCGTC222800.044.5062350-51
TATGCCG222300.044.47334348-49
TCTCGTA217200.044.2922242-43
CCGTCTT223500.044.240952-53
CGTATGC223000.044.23710346-47
Produced by FastQC (version 0.11.2)