##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34098-1.34100000035707.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2352785 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6653442622254 33.0 33.0 33.0 33.0 33.0 2 32.69718525067101 33.0 33.0 33.0 33.0 33.0 3 32.7303102493428 33.0 33.0 33.0 33.0 33.0 4 36.64950558593327 37.0 37.0 37.0 37.0 37.0 5 36.65637191668597 37.0 37.0 37.0 37.0 37.0 6 36.552711786244814 37.0 37.0 37.0 37.0 37.0 7 36.438967861491804 37.0 37.0 37.0 37.0 37.0 8 36.39432119806952 37.0 37.0 37.0 37.0 37.0 9 36.43697235404 37.0 37.0 37.0 37.0 37.0 10-11 36.3617313099157 37.0 37.0 37.0 37.0 37.0 12-13 36.353193768236366 37.0 37.0 37.0 37.0 37.0 14-15 38.23807742738924 40.0 37.0 40.0 37.0 40.0 16-17 38.25126244004446 40.0 37.0 40.0 37.0 40.0 18-19 38.21644157880979 40.0 37.0 40.0 37.0 40.0 20-21 38.220393278603865 40.0 37.0 40.0 37.0 40.0 22-23 38.02257261075704 40.0 37.0 40.0 33.0 40.0 24-25 38.10192346517 40.0 37.0 40.0 37.0 40.0 26-27 38.03177043376254 40.0 37.0 40.0 37.0 40.0 28-29 37.868356012130306 40.0 37.0 40.0 33.0 40.0 30-31 37.787772363390616 40.0 37.0 40.0 33.0 40.0 32-33 37.71498670724269 40.0 37.0 40.0 33.0 40.0 34-35 37.600290294268284 40.0 37.0 40.0 33.0 40.0 36-37 37.5407701936216 40.0 37.0 40.0 33.0 40.0 38-39 37.494120584753816 40.0 37.0 40.0 33.0 40.0 40-41 37.42242873870753 40.0 37.0 40.0 33.0 40.0 42-43 37.00821026995666 38.5 37.0 40.0 33.0 40.0 44-45 36.89064491655634 37.0 37.0 40.0 33.0 40.0 46-47 36.97524890714621 37.0 37.0 40.0 33.0 40.0 48-49 36.83679405470538 37.0 37.0 40.0 33.0 40.0 50-51 36.74052686497066 37.0 37.0 40.0 33.0 40.0 52-53 36.63104682323289 37.0 37.0 40.0 33.0 40.0 54-55 36.40468657357132 37.0 37.0 40.0 33.0 40.0 56-57 36.24224525402873 37.0 37.0 40.0 33.0 40.0 58-59 35.998307112634606 37.0 37.0 40.0 33.0 40.0 60-61 35.746940328164285 37.0 35.0 40.0 30.0 40.0 62-63 35.40481068180901 37.0 33.0 38.5 27.0 40.0 64-65 35.10239163374469 37.0 33.0 37.0 27.0 40.0 66-67 34.58112258451155 37.0 33.0 37.0 27.0 40.0 68-69 34.31506193723608 37.0 33.0 37.0 27.0 40.0 70-71 33.890702720393065 37.0 33.0 37.0 27.0 37.0 72-73 33.086493878531186 37.0 33.0 37.0 24.5 37.0 74-75 32.35359690749473 37.0 33.0 37.0 22.0 37.0 76-77 29.686293902757797 33.0 27.0 33.0 18.5 37.0 78-79 31.972566129076817 33.0 33.0 37.0 22.0 37.0 80-81 32.302148942636066 37.0 33.0 37.0 22.0 37.0 82-83 32.177594425329985 37.0 33.0 37.0 18.5 37.0 84-85 32.04575853722291 37.0 33.0 37.0 15.0 37.0 86-87 31.945648454916196 37.0 33.0 37.0 15.0 37.0 88-89 31.69018652363051 33.0 33.0 37.0 15.0 37.0 90-91 31.682037670250363 33.0 33.0 37.0 15.0 37.0 92-93 31.5357707992868 33.0 33.0 37.0 15.0 37.0 94-95 31.368307346400115 33.0 33.0 37.0 15.0 37.0 96-97 31.251241401148 33.0 33.0 37.0 15.0 37.0 98-99 31.127919253140426 33.0 33.0 37.0 15.0 37.0 100-101 29.580847166230658 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.14155092115351664 2306 2 0.14944001579100075 2306 3 0.12190100239951107 2306 4 0.1619394582436584 2306 5 0.15606211859059727 2306 6 0.1655586497252557 2306 7 0.17559169538490238 2306 8 0.22604672553811866 2306 9 0.24230283030055233 2306 10-11 0.25471839230065285 2306 12-13 0.1931846183563053 2306 14-15 0.37689211287035107 2306 16-17 0.3479077082243336 2306 18-19 0.3216197651528816 2306 20-21 0.40517565594327465 2306 22-23 0.44524199160977673 2306 24-25 0.4020945168185577 2306 26-27 0.35924861300501476 2306 28-29 0.4621020894188419 2306 30-31 0.5220929399882479 2306 32-33 0.4186189092314976 2306 34-35 0.539006784960975 2306 36-37 0.33922242361592936 2306 38-39 0.5409051837536438 2306 40-41 0.5139311471842376 2306 42-43 0.38779345107563046 2306 44-45 0.34860779961037736 2306 46-47 0.4071374090375244 2306 48-49 0.35515817574253106 2306 50-51 0.3636862342433247 2306 52-53 0.4439956054294356 2306 54-55 0.3285039736269084 2306 56-57 0.3437077650268634 2306 58-59 0.26601444818028597 2306 60-61 0.23410649938372075 2306 62-63 0.25821995036323386 2306 64-65 0.23759328574676175 2306 66-67 0.3045198328078058 2306 68-69 0.4016198467006902 2306 70-71 0.11766684545911943 2306 72-73 -0.17866551290507715 2306 74-75 -0.15715789123365198 2306 76-77 -0.3693126333976302 2306 78-79 0.03753613237318376 2306 80-81 0.12950292244946127 2306 82-83 0.24218640345740283 2306 84-85 0.2112040555489756 2306 86-87 0.09062988607657729 2306 88-89 0.29084113355838426 2306 90-91 0.08649824016148244 2306 92-93 0.2748702909774856 2306 94-95 0.2170662955114082 2306 96-97 0.18402997710817814 2306 98-99 0.3157089608084931 2306 100-101 0.173942676776921 2307 1 0.18844643109019188 2307 2 0.15699091283745048 2307 3 0.15103232962292168 2307 4 0.18452528036145566 2307 5 0.1871774231716259 2307 6 0.16484312173689375 2307 7 0.20564652209102974 2307 8 0.3084057939200875 2307 9 0.15132561172191572 2307 10-11 0.1806394467927248 2307 12-13 0.21101743755425417 2307 14-15 0.3120284255180792 2307 16-17 0.35898763997668937 2307 18-19 0.2893541366547865 2307 20-21 0.3611671497324025 2307 22-23 0.35875633869711265 2307 24-25 0.44176449021234276 2307 26-27 0.39280537331581655 2307 28-29 0.30586527174798306 2307 30-31 0.40021148138341545 2307 32-33 0.37468537291485404 2307 34-35 0.43972042765533814 2307 36-37 0.4328305110558617 2307 38-39 0.4627679956225208 2307 40-41 0.46856758591101055 2307 42-43 0.5458018559519431 2307 44-45 0.4243715793420506 2307 46-47 0.4621061013740473 2307 48-49 0.3999299314461382 2307 50-51 0.5259412638542287 2307 52-53 0.4078424417882687 2307 54-55 0.4622539471149594 2307 56-57 0.5066014379802013 2307 58-59 0.5697845059409801 2307 60-61 0.5660238492730798 2307 62-63 0.5362416053214432 2307 64-65 0.41215972704224413 2307 66-67 0.29893538577631773 2307 68-69 0.33991196475779617 2307 70-71 0.27365091590313284 2307 72-73 0.22780623007278678 2307 74-75 0.3881256923331762 2307 76-77 -0.055036185668583926 2307 78-79 0.3888759872526819 2307 80-81 0.42811110415409814 2307 82-83 0.4876251702865986 2307 84-85 0.4077453658754351 2307 86-87 0.36107943443083457 2307 88-89 0.6515579694694615 2307 90-91 0.6308562622052811 2307 92-93 0.4552756939944267 2307 94-95 0.37554293082720847 2307 96-97 0.4556425161985729 2307 98-99 0.38715585914637174 2307 100-101 0.40168722297487136 2201 1 0.16523567889940693 2201 2 0.15751573030776456 2201 3 0.1500657860228074 2201 4 0.17285879929400494 2201 5 0.19685542281185064 2201 6 0.20930012982044843 2201 7 0.22228135135403448 2201 8 0.18584663773977184 2201 9 0.22099555921161596 2201 10-11 0.2665460418466452 2201 12-13 0.2947609451509763 2201 14-15 0.42910526644813274 2201 16-17 0.4554739613146026 2201 18-19 0.41027645053446093 2201 20-21 0.5007848724586026 2201 22-23 0.46497091423171355 2201 24-25 0.4403280950630517 2201 26-27 0.464148569105852 2201 28-29 0.5961687121863477 2201 30-31 0.5873464576980965 2201 32-33 0.6462401518090815 2201 34-35 0.5903370453523706 2201 36-37 0.5902915994667595 2201 38-39 0.6039004934738514 2201 40-41 0.6169570130376201 2201 42-43 0.6827996316167386 2201 44-45 0.6515842788968982 2201 46-47 0.7505918116622325 2201 48-49 0.70467441843563 2201 50-51 0.7061578153066179 2201 52-53 0.6711050760978594 2201 54-55 0.7284950205065996 2201 56-57 0.679039954671282 2201 58-59 0.7788358940803377 2201 60-61 0.5337659920017401 2201 62-63 0.8609561394531369 2201 64-65 0.6626966798457872 2201 66-67 0.6771027791352893 2201 68-69 0.6227714490204121 2201 70-71 0.4988644263837756 2201 72-73 0.38498135292518754 2201 74-75 0.44783852751822906 2201 76-77 0.3290382596039194 2201 78-79 0.44015390922677256 2201 80-81 0.4667444372018963 2201 82-83 0.6508229876398346 2201 84-85 0.7054867662072297 2201 86-87 0.5373871236635068 2201 88-89 0.8497018345588252 2201 90-91 0.4079730212432544 2201 92-93 0.4786846090385275 2201 94-95 0.528255963032958 2201 96-97 0.46224328086733735 2201 98-99 0.45496781573328704 2201 100-101 0.599052713513597 1101 1 0.18013945751999927 1101 2 0.10999969341119709 1101 3 0.1345205315395006 1101 4 0.19146159931273132 1101 5 0.16253880530938858 1101 6 0.1501111051447026 1101 7 0.18443427545386015 1101 8 0.1671548739152655 1101 9 0.16646542857529312 1101 10-11 0.16542228851050567 1101 12-13 0.17818792459565458 1101 14-15 0.29984788011110197 1101 16-17 0.2979775901014179 1101 18-19 0.2767815776590581 1101 20-21 0.34144601400469554 1101 22-23 0.3272572840402006 1101 24-25 0.3681526958965051 1101 26-27 0.38425204805888313 1101 28-29 0.3406576385336777 1101 30-31 0.35912620204421586 1101 32-33 0.358589732742729 1101 34-35 0.3731951246706231 1101 36-37 0.3264413395988015 1101 38-39 0.4783678812155685 1101 40-41 0.4061362048716788 1101 42-43 0.28351836552131005 1101 44-45 0.43475841384094593 1101 46-47 0.49679446184718756 1101 48-49 0.4554727515348489 1101 50-51 0.44522931411262334 1101 52-53 0.3066905984090411 1101 54-55 0.3473015008175082 1101 56-57 0.4516835843945515 1101 58-59 0.5727395322317577 1101 60-61 0.44983566470857284 1101 62-63 0.5328412356745176 1101 64-65 0.4032594929749749 1101 66-67 0.3935470362875151 1101 68-69 0.3927555291814784 1101 70-71 0.25431463006406574 1101 72-73 0.08336361635169709 1101 74-75 0.07762267323157346 1101 76-77 -0.15202883783486243 1101 78-79 0.1222885723348881 1101 80-81 0.23229905629633407 1101 82-83 0.45082298763983175 1101 84-85 0.3739397324478091 1101 86-87 0.34979289226396304 1101 88-89 0.5099256487649306 1101 90-91 0.4566338818510083 1101 92-93 0.4596533780603913 1101 94-95 0.46001370054290547 1101 96-97 0.5585957648304394 1101 98-99 0.3407593594894287 1101 100-101 0.41946288348128746 2304 1 0.1570373480206655 2304 2 0.07166359084737906 2304 3 0.055762418523563895 2304 4 0.0876136130178864 2304 5 0.09120093983777622 2304 6 0.06227811784998494 2304 7 0.028268567650528098 2304 8 0.1545652425408548 2304 9 0.07703310700174626 2304 10-11 0.07298896890812756 2304 12-13 0.0863731893405415 2304 14-15 0.2300271013905899 2304 16-17 0.18989673594356304 2304 18-19 0.16008454621884738 2304 20-21 0.2055410746374875 2304 22-23 0.2206829189127859 2304 24-25 0.2677180971953206 2304 26-27 0.21092586588314788 2304 28-29 0.17267109487429622 2304 30-31 0.24944163628812532 2304 32-33 0.1495407076869526 2304 34-35 0.25282966918519634 2304 36-37 0.2616383512258835 2304 38-39 0.33344681580367563 2304 40-41 0.24417792118636328 2304 42-43 0.43701805542279004 2304 44-45 0.26408880377255883 2304 46-47 0.35334417575480614 2304 48-49 0.25864911880208297 2304 50-51 0.30133714914574483 2304 52-53 0.26872610722404744 2304 54-55 0.2874517949272928 2304 56-57 0.16684956773862325 2304 58-59 0.3819390909979603 2304 60-61 0.2611614487786298 2304 62-63 0.26482695363322506 2304 64-65 0.20167656212463925 2304 66-67 0.2736234596765925 2304 68-69 0.1657984296762578 2304 70-71 0.20036693304092523 2304 72-73 -0.04973200549951429 2304 74-75 0.1056386693389868 2304 76-77 -0.07149307237071412 2304 78-79 0.18158466251319538 2304 80-81 0.20757964247008687 2304 82-83 0.238805909643375 2304 84-85 0.2365465044834707 2304 86-87 0.2779751343546053 2304 88-89 0.6648716971513657 2304 90-91 0.3872639670290461 2304 92-93 0.340774828860706 2304 94-95 0.40362576314502263 2304 96-97 0.3861321449623851 2304 98-99 0.38924876960186694 2304 100-101 0.3055581210499305 2202 1 0.10134879325942592 2202 2 0.11659748957309546 2202 3 0.08438228963715488 2202 4 -0.007109682923214677 2202 5 0.010317255456797625 2202 6 -0.0052728382174080934 2202 7 0.08293195803492637 2202 8 0.09698227347384858 2202 9 0.02985210473636357 2202 10-11 0.051761700696999924 2202 12-13 0.008900482971100132 2202 14-15 -0.05094213163505401 2202 16-17 -0.07570739705813168 2202 18-19 -3.389475293076316E-4 2202 20-21 0.045321344943673125 2202 22-23 0.030023205098601125 2202 24-25 0.012978405505926105 2202 26-27 0.09284626306283172 2202 28-29 0.022541180504660474 2202 30-31 -0.007577309575367508 2202 32-33 0.09962442836912544 2202 34-35 0.07194600630965908 2202 36-37 -0.007241490562883257 2202 38-39 0.03721173036471015 2202 40-41 0.028980391559386476 2202 42-43 0.14791675490274514 2202 44-45 0.18410307908435897 2202 46-47 0.14655204777850628 2202 48-49 0.1407268282833627 2202 50-51 0.13320750316400876 2202 52-53 0.10143657129855654 2202 54-55 0.039239112136421284 2202 56-57 0.0526648153544258 2202 58-59 0.09676715714697792 2202 60-61 0.08962931943458585 2202 62-63 0.12823288646829667 2202 64-65 -0.029465639163710478 2202 66-67 -0.010516535583612097 2202 68-69 -0.01340479803238992 2202 70-71 -0.15316832491469512 2202 72-73 -0.2756372017732218 2202 74-75 -0.12140674542140317 2202 76-77 -0.39539652068216924 2202 78-79 -0.04931312943714872 2202 80-81 -0.12712695664655627 2202 82-83 0.004198644159131959 2202 84-85 0.11482506274148818 2202 86-87 0.27388619731264896 2202 88-89 0.28486807993617447 2202 90-91 -0.0014667732355526653 2202 92-93 0.17556852501376952 2202 94-95 0.22533898980850253 2202 96-97 0.11561541652492124 2202 98-99 0.001207171197055601 2202 100-101 0.050965634104809254 1102 1 0.06168107684657542 1102 2 0.09976945551201766 1102 3 0.08361038564514445 1102 4 0.1417236636535648 1102 5 0.09432303176355816 1102 6 -0.03332611580933076 1102 7 0.12445524407562658 1102 8 0.10660168102131706 1102 9 0.10716696534768744 1102 10-11 0.08954719165874536 1102 12-13 0.0856547604705824 1102 14-15 0.14117104878776843 1102 16-17 0.16435018357421427 1102 18-19 0.11588388785568071 1102 20-21 0.12978584465745513 1102 22-23 0.1284108622564588 1102 24-25 0.13579708752850905 1102 26-27 0.22487944763708612 1102 28-29 0.17821878977529337 1102 30-31 0.21979149107070128 1102 32-33 0.16578742497826227 1102 34-35 0.16850089825120307 1102 36-37 0.09979998468185869 1102 38-39 0.2801781403787018 1102 40-41 0.24386869251113552 1102 42-43 0.14862754307186066 1102 44-45 0.16566363462819567 1102 46-47 0.29569786546970533 1102 48-49 0.24334830180915645 1102 50-51 0.2005952487016387 1102 52-53 0.15223507030205496 1102 54-55 0.10798863577760187 1102 56-57 0.09571082796759356 1102 58-59 0.29933550146156307 1102 60-61 0.26118690736434047 1102 62-63 0.2079659268781242 1102 64-65 0.15082603838150987 1102 66-67 0.1727613036365767 1102 68-69 0.25894332255977304 1102 70-71 0.14678985686221324 1102 72-73 0.30433979516349297 1102 74-75 0.2069327934146088 1102 76-77 0.2261892028237682 1102 78-79 0.18652756076826194 1102 80-81 0.19514012213468135 1102 82-83 0.31755345485616715 1102 84-85 0.4494682476887135 1102 86-87 0.4655411610931637 1102 88-89 0.4238418124973826 1102 90-91 0.10695127764200052 1102 92-93 0.3413098731213928 1102 94-95 0.3352286853767019 1102 96-97 0.11452070341860932 1102 98-99 0.10093856563512915 1102 100-101 0.1011910835848262 2305 1 0.060305423969154504 2305 2 0.07178941958144947 2305 3 0.03654967250669472 2305 4 0.10713008356528775 2305 5 0.07766350361993091 2305 6 -0.015423294902973339 2305 7 -0.01874798967531177 2305 8 0.09943490132803845 2305 9 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-0.9115700903153154 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 34.0 9 75.0 10 79.0 11 38.0 12 46.0 13 63.0 14 179.0 15 467.0 16 850.0 17 1529.0 18 2108.0 19 2816.0 20 3906.0 21 5276.0 22 7420.0 23 9840.0 24 13366.0 25 17732.0 26 24088.0 27 34496.0 28 56810.0 29 86349.0 30 69697.0 31 51628.0 32 65463.0 33 92567.0 34 142623.0 35 253449.0 36 511871.0 37 745998.0 38 151847.0 39 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.695187962562876 24.427747067375336 20.366410458896425 14.510654511165367 2 29.936734550755805 27.4901446583517 28.92002456663061 13.653096224261885 3 34.908988438348196 18.121216706378586 30.415406808462166 16.55438804681105 4 29.89550341590746 17.561930593521893 28.94194020142588 23.600625789144768 5 36.395590757336514 16.230722314193606 30.26324122263615 17.110445705833722 6 32.86679403345397 17.136287421077572 33.03174748223913 16.965171063229324 7 31.77179385281698 24.24084648618552 25.510193239076244 18.477166421921254 8 29.062281508935158 30.652057030285384 22.06487205588271 18.22078940489675 9 33.23134073024097 23.379696827376918 24.15643588343176 19.23252655895035 10-11 25.947637912912054 29.358020818855724 27.971549292302566 16.72279197592966 12-13 22.339482783169736 28.45206850604709 27.649041455126582 21.559407255656595 14-15 22.235669642572525 28.626351324069134 27.96286103490119 21.175117998457146 16-17 25.24939289478097 25.451368146437158 28.51465946322272 20.784579495559157 18-19 20.805449711724616 25.473322041750524 32.0490610064243 21.672167240100563 20-21 24.003000699171405 25.82970819688157 31.728972260533794 18.43831884341323 22-23 20.469917038461357 32.507521962344924 28.290738986312768 18.73182201288095 24-25 20.75737902103252 24.805411459185603 32.08370080564097 22.3535087141409 26-27 20.48831491190228 24.87532859993582 28.929013913298498 25.707342574863407 28-29 23.388006128906806 29.177804176752232 28.255917986556355 19.178271707784607 30-31 19.328009146607105 26.96327968768927 31.13410277607176 22.574608389631862 32-33 19.07634994272745 30.475564065564853 28.26739374826004 22.18069224344766 34-35 19.61024913028602 30.437120263857516 31.89556206793226 18.057068537924202 36-37 19.193219192114107 31.080714633634226 32.22615755636576 17.499908617885904 38-39 19.579657348717745 27.204900094399324 31.461285437469265 21.754157119413662 40-41 23.153045838199244 26.49005229148492 27.966433988508836 22.390467881807005 42-43 19.976839444945757 30.04305253480353 31.310804957863432 18.669303062387286 44-45 19.47582950273402 26.88411294795698 31.536107516050265 22.10395003325874 46-47 22.996124843210655 26.693208040705933 28.339480226584246 21.97118688949916 48-49 19.644777124956033 30.804288433153427 31.0590270582747 18.49190738361584 50-51 22.939513030290666 27.293328606792617 30.38394217298459 19.383216189932128 52-53 19.269685963901896 26.836134659154904 27.501907338418153 26.39227203852505 54-55 23.273497425128955 26.74327164836379 31.00727084922924 18.97596007727801 56-57 19.991544668720206 26.77334525893549 30.35240749510492 22.88270257723939 58-59 19.847406778227132 26.931253977545914 30.627666842241975 22.59367240198498 60-61 22.749789109977154 27.17009499774604 30.68658650189084 19.393529390385964 62-63 19.428844055258214 27.216911112315362 30.51956787152179 22.834676960904634 64-65 23.294756174516888 27.166237720627006 30.316541560652276 19.22246454420383 66-67 20.383930456211047 33.6173522739762 26.861143925770865 19.137573344041883 68-69 20.62457462514937 33.515669736096484 26.88570982814112 18.97404581061302 70-71 20.065489961897793 34.37149428551931 26.776520979173355 18.786494773409537 72-73 19.921449352135717 34.329621918674825 26.669700817945397 19.07922791124406 74-75 20.72133603642499 33.01202765248309 26.54280401756457 19.723832293527355 76-77 20.755447814230827 32.03877922361193 26.862709957930285 20.343063004226956 78-79 20.558301566164804 31.708030407211353 27.389976842823454 20.34369118380039 80-81 19.818218684232118 31.726459162328368 27.653510291405258 20.801811862034256 82-83 19.156904185833007 31.535230468928482 27.875779803060873 21.43208554217764 84-85 19.247078016683567 31.242315534029075 28.12931645154236 21.381289997745004 86-87 19.210827307522834 31.41157112907878 28.807515501242076 20.57008606215631 88-89 19.472305946070033 31.687213845113956 28.66410039493094 20.176379813885077 90-91 20.227447561031862 31.02569067837412 27.82184344290503 20.92501831768899 92-93 19.60123665874231 31.609909937873677 27.582605138741428 21.20624826464259 94-95 19.487935147351607 31.586140076176694 27.69978101007456 21.226143766397144 96-97 19.930845894246 32.205338292226045 27.097347179459707 20.76646863406824 98-99 20.151188763586802 31.510712020886405 27.117334666122154 21.220764549404635 100-101 20.176902933999884 31.574570017258825 26.741483238537334 21.50704381020396 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 52.0 1 36.5 2 32.5 3 63.0 4 65.5 5 44.5 6 41.0 7 40.5 8 42.5 9 46.5 10 52.0 11 56.5 12 51.0 13 52.0 14 72.0 15 68.0 16 71.0 17 92.0 18 109.0 19 147.5 20 170.0 21 189.5 22 231.5 23 338.5 24 509.5 25 704.5 26 1082.5 27 1757.5 28 2456.5 29 3230.0 30 4243.0 31 5753.5 32 10005.5 33 18827.5 34 34308.0 35 56498.0 36 87471.0 37 127619.0 38 160816.0 39 177465.0 40 184877.0 41 175571.0 42 162986.5 43 162168.5 44 154209.0 45 139267.5 46 129063.0 47 113158.5 48 89509.0 49 73026.5 50 61491.0 51 49586.0 52 38649.0 53 30499.0 54 23495.0 55 16639.0 56 11985.0 57 9372.0 58 7551.5 59 5898.5 60 4403.5 61 3372.5 62 2587.0 63 1925.0 64 1530.5 65 1175.0 66 887.0 67 734.0 68 661.5 69 515.5 70 337.0 71 238.5 72 156.5 73 108.0 74 81.5 75 59.5 76 41.0 77 25.0 78 21.5 79 12.5 80 6.0 81 4.5 82 4.0 83 3.5 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16066066385156313 2 0.0 3 2.9751974787326505E-4 4 0.023589065724237444 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.950394957465301E-4 12-13 0.0 14-15 0.0 16-17 9.138106541821714E-4 18-19 0.0 20-21 0.0 22-23 3.4002256899801726E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0011475761703683082 38-39 5.950394957465301E-4 40-41 0.004569053270910856 42-43 0.003315220047730668 44-45 8.50056422495043E-4 46-47 0.004505299039223728 48-49 0.0075442507496435085 50-51 0.004802818787096994 52-53 0.0012750846337425646 54-55 0.010200677069940517 56-57 0.017978693335770162 58-59 0.022016461342621616 60-61 0.010859470797374177 62-63 5.31285264059402E-4 64-65 0.013324634422609801 66-67 0.032748423676621535 68-69 0.019041263863888963 70-71 0.034916067553983895 72-73 0.028561895795833448 74-75 0.028200621816273058 76-77 0.03636116347222547 78-79 0.016257329080217698 80-81 0.012240812483928621 82-83 0.0011688275809306843 84-85 0.009796900269255372 86-87 0.020125085802570143 88-89 0.020188840034257274 90-91 0.023397803029176063 92-93 0.04097271956426107 94-95 0.026436754739595843 96-97 0.02749932526771464 98-99 0.035043576017358156 100-101 0.015513529710534536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2352785.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.25387280641255 #Duplication Level Percentage of deduplicated Percentage of total 1 84.34388025546907 28.891045462701285 2 6.381340642215148 4.371712613856573 3 2.463774479751481 2.5318145295927743 4 1.4901597498911114 2.0417497013602266 5 0.8061448188836757 1.3806791044794955 6 0.7023070181800374 1.443404116307392 7 0.4326999598231179 1.037515457098463 8 0.31741060587808145 0.8698034016923315 9 0.305504378700086 0.9418237316815354 >10 2.1074056181728165 14.288092428667474 >50 0.3220523306250766 7.776778386596615 >100 0.30295248373712247 21.12524509532384 >500 0.01884493975322302 4.211681809991504 >1k 0.005397774457325926 2.9312174572336502 >5k 0.0 0.0 >10k+ 1.249444625083876E-4 6.157436703416811 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 143871 6.114923378039217 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 4357 0.18518479164054513 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3146 0.13371387525847028 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3015 0.12814600569112775 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2905 0.123470695367405 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2589 0.11003980389198334 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2589 0.11003980389198334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004377790575849472 0.0 0.0 2 0.004760315965972242 0.0 0.0 3 0.0053128526405940195 0.0 0.0 4 0.005737880851841541 0.0 0.0 5 0.006885457022209849 0.0 0.0 6 0.008415558582700928 0.0 0.0 7 0.009648140395318739 0.0 0.0 8 0.010923225029061304 0.0 0.0 9 0.012580835052926637 0.0 0.0 10-11 0.019870068875821635 0.0 0.0 12-13 0.02758433090996415 0.0 0.0 14-15 0.033917251257552225 0.0 0.0 16-17 0.03999515467839178 0.0 2.125141056237608E-5 18-19 0.046264320794292726 0.0 4.250282112475216E-5 20-21 0.053447297564375836 0.0 4.250282112475216E-5 22-23 0.06001398342815004 0.0 4.250282112475216E-5 24-25 0.0667081777552985 0.0 4.250282112475216E-5 26-27 0.07423117709437964 0.0 4.250282112475216E-5 28-29 0.08268923849820532 0.0 4.250282112475216E-5 30-31 0.13855919686669205 0.0 4.250282112475216E-5 32-33 0.15090626640343252 0.0 4.250282112475216E-5 34-35 0.16087317795718692 0.0 4.250282112475216E-5 36-37 0.1730502362094284 0.0 4.250282112475216E-5 38-39 0.18637487063203823 0.0 4.250282112475216E-5 40-41 0.20063456711939254 0.0 4.250282112475216E-5 42-43 0.21874076891853697 0.0 4.250282112475216E-5 44-45 0.2390571174161685 0.0 4.250282112475216E-5 46-47 0.2601172652834832 0.0 4.250282112475216E-5 48-49 0.2851514269259622 0.0 4.250282112475216E-5 50-51 0.3115456788444333 0.0 4.250282112475216E-5 52-53 0.34140391068457165 0.0 4.250282112475216E-5 54-55 0.3724947243373279 0.0 4.250282112475216E-5 56-57 0.4061357072575692 0.0 4.250282112475216E-5 58-59 0.4452383026923412 2.125141056237608E-5 4.250282112475216E-5 60-61 0.48601975956154087 4.250282112475216E-5 4.250282112475216E-5 62-63 0.5310940013643406 4.250282112475216E-5 4.250282112475216E-5 64-65 0.5758069691875798 4.250282112475216E-5 4.250282112475216E-5 66-67 0.624132676806423 4.250282112475216E-5 8.500564224950431E-5 68-69 0.6756460960096227 4.250282112475216E-5 8.500564224950431E-5 70-71 0.7311972832196737 4.250282112475216E-5 8.500564224950431E-5 72-73 0.7901486961197048 4.250282112475216E-5 8.500564224950431E-5 74-75 0.8539879334490827 4.250282112475216E-5 8.500564224950431E-5 76-77 0.9211848936473159 4.250282112475216E-5 8.500564224950431E-5 78-79 0.9929721585270221 4.250282112475216E-5 8.500564224950431E-5 80-81 1.0704973042585701 4.250282112475216E-5 8.500564224950431E-5 82-83 1.1576493389748745 4.250282112475216E-5 8.500564224950431E-5 84-85 1.2501354777423352 4.250282112475216E-5 8.500564224950431E-5 86-87 1.3478707149187028 4.250282112475216E-5 8.500564224950431E-5 88-89 1.4495587144596722 4.250282112475216E-5 8.500564224950431E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 20775 0.0 75.24832 3 GATCGGA 20925 0.0 75.0085 1 CGGAAGA 20800 0.0 74.99802 4 GAAGAGC 20935 0.0 74.49538 6 ATCGGAA 21035 0.0 74.3408 2 GGAAGAG 21605 0.0 72.40499 5 AACGCCG 70 1.8189894E-11 61.16994 1 ACCGCGT 25 0.005021181 57.002144 3 GAGCACA 28250 0.0 55.49147 9 AGAGCAC 28320 0.0 55.203392 8 AAGAGCA 28475 0.0 54.78615 7 CGTCTTC 17265 0.0 45.13804 52-53 GCCGTCT 17320 0.0 45.115726 50-51 ATGCCGT 17295 0.0 45.049686 48-49 CTGCTTG 17520 0.0 44.832596 58-59 TCGTATG 17445 0.0 44.756664 44-45 TGCCGTC 17335 0.0 44.63825 50-51 CTTGAAA 16675 0.0 44.574276 62-63 GTATGCC 17530 0.0 44.55319 46-47 TATGCCG 17365 0.0 44.526207 48-49 >>END_MODULE