FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34098-1.34100000035707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34098-1.34100000035707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352785
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1438716.114923378039217TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT43570.18518479164054513TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC31460.13371387525847028No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA30150.12814600569112775No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG29050.123470695367405No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC25890.11003980389198334No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25890.11003980389198334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG207750.075.248323
GATCGGA209250.075.00851
CGGAAGA208000.074.998024
GAAGAGC209350.074.495386
ATCGGAA210350.074.34082
GGAAGAG216050.072.404995
AACGCCG701.8189894E-1161.169941
ACCGCGT250.00502118157.0021443
GAGCACA282500.055.491479
AGAGCAC283200.055.2033928
AAGAGCA284750.054.786157
CGTCTTC172650.045.1380452-53
GCCGTCT173200.045.11572650-51
ATGCCGT172950.045.04968648-49
CTGCTTG175200.044.83259658-59
TCGTATG174450.044.75666444-45
TGCCGTC173350.044.6382550-51
CTTGAAA166750.044.57427662-63
GTATGCC175300.044.5531946-47
TATGCCG173650.044.52620748-49
Produced by FastQC (version 0.11.2)