##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34042-1.3410000003584a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5452897 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.666198352912225 33.0 33.0 33.0 33.0 33.0 2 32.7019105624038 33.0 33.0 33.0 33.0 33.0 3 32.74085481533944 33.0 33.0 33.0 33.0 33.0 4 36.653981544122324 37.0 37.0 37.0 37.0 37.0 5 36.66487025153785 37.0 37.0 37.0 37.0 37.0 6 36.56061741125864 37.0 37.0 37.0 37.0 37.0 7 36.44681955298257 37.0 37.0 37.0 37.0 37.0 8 36.41237309268816 37.0 37.0 37.0 37.0 37.0 9 36.43738896956975 37.0 37.0 37.0 37.0 37.0 10-11 36.38122671306647 37.0 37.0 37.0 37.0 37.0 12-13 36.37435596161086 37.0 37.0 37.0 37.0 37.0 14-15 38.27345069969229 40.0 37.0 40.0 37.0 40.0 16-17 38.28804385998855 40.0 37.0 40.0 37.0 40.0 18-19 38.26581805964793 40.0 37.0 40.0 37.0 40.0 20-21 38.26491642882673 40.0 37.0 40.0 37.0 40.0 22-23 38.047848235534246 40.0 37.0 40.0 37.0 40.0 24-25 38.14495368241872 40.0 37.0 40.0 37.0 40.0 26-27 38.101322838117056 40.0 37.0 40.0 37.0 40.0 28-29 37.928670759781454 40.0 37.0 40.0 33.0 40.0 30-31 37.87109897729592 40.0 37.0 40.0 33.0 40.0 32-33 37.720279513807064 40.0 37.0 40.0 33.0 40.0 34-35 37.65635853748934 40.0 37.0 40.0 33.0 40.0 36-37 37.6640152014608 40.0 37.0 40.0 33.0 40.0 38-39 37.49482486098674 40.0 37.0 40.0 33.0 40.0 40-41 37.2478879575389 40.0 37.0 40.0 33.0 40.0 42-43 37.0706187004816 38.5 37.0 40.0 33.0 40.0 44-45 37.00198169890243 37.0 37.0 40.0 33.0 40.0 46-47 37.03163859504407 37.0 37.0 40.0 33.0 40.0 48-49 36.86077960027487 37.0 37.0 40.0 33.0 40.0 50-51 36.777031548551165 37.0 37.0 40.0 33.0 40.0 52-53 36.657032674558124 37.0 37.0 40.0 33.0 40.0 54-55 36.41310224638389 37.0 37.0 40.0 33.0 40.0 56-57 36.263860751450096 37.0 37.0 40.0 33.0 40.0 58-59 36.046690960786535 37.0 37.0 40.0 33.0 40.0 60-61 35.777795362721875 37.0 37.0 40.0 30.0 40.0 62-63 35.534319280191795 37.0 33.0 40.0 27.0 40.0 64-65 35.1781801490107 37.0 33.0 37.0 27.0 40.0 66-67 34.52710751734354 37.0 33.0 37.0 27.0 40.0 68-69 34.28493166109685 37.0 33.0 37.0 27.0 40.0 70-71 33.80798426964603 37.0 33.0 37.0 27.0 37.0 72-73 32.86053358425805 37.0 33.0 37.0 22.0 37.0 74-75 32.00437895672704 37.0 33.0 37.0 18.5 37.0 76-77 29.36542685475262 33.0 27.0 33.0 18.5 37.0 78-79 31.580838405713514 33.0 33.0 37.0 18.5 37.0 80-81 31.86727532172348 37.0 33.0 37.0 15.0 37.0 82-83 31.73887082407755 37.0 33.0 37.0 15.0 37.0 84-85 31.59202952118846 37.0 33.0 37.0 15.0 37.0 86-87 31.4910466858259 33.0 33.0 37.0 15.0 37.0 88-89 31.25615924892768 33.0 33.0 37.0 15.0 37.0 90-91 31.224566409378355 33.0 33.0 37.0 15.0 37.0 92-93 31.068472501864605 33.0 33.0 37.0 15.0 37.0 94-95 30.90027566997873 33.0 33.0 37.0 15.0 37.0 96-97 30.787156441795986 33.0 33.0 37.0 15.0 37.0 98-99 30.65936748851115 33.0 33.0 37.0 15.0 37.0 100-101 29.14977185521751 33.0 27.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.15928694308605884 2306 2 0.17447939265158396 2306 3 0.1270549582773839 2306 4 0.17897642522392943 2306 5 0.17801041378429971 2306 6 0.13119536213451255 2306 7 0.23950858648635176 2306 8 0.1798126271174212 2306 9 0.2209236722546848 2306 10-11 0.25079739217679986 2306 12-13 0.2613540330798685 2306 14-15 0.48735996916742863 2306 16-17 0.4882834216735077 2306 18-19 0.44087418482144614 2306 20-21 0.40239594095995557 2306 22-23 0.43059731074681906 2306 24-25 0.45863419064912847 2306 26-27 0.4405599057141032 2306 28-29 0.4608258504839142 2306 30-31 0.4997059154491339 2306 32-33 0.5397109822782511 2306 34-35 0.6478024991589706 2306 36-37 0.4812486102565856 2306 38-39 0.5887961620331268 2306 40-41 0.6280723780881132 2306 42-43 0.6693938103329558 2306 44-45 0.6237573099013503 2306 46-47 0.7607065567689801 2306 48-49 0.7056476826176592 2306 50-51 0.6660793313277509 2306 52-53 0.5778673904752836 2306 54-55 0.6044658584303875 2306 56-57 0.603757353955686 2306 58-59 0.6229455076984465 2306 60-61 0.6533546589104802 2306 62-63 0.6230606608175933 2306 64-65 0.6356784562721813 2306 66-67 0.5758734550462421 2306 68-69 0.4980909764023238 2306 70-71 0.36309828930912147 2306 72-73 0.25931213653960583 2306 74-75 0.29607533385791385 2306 76-77 2.934276339914277E-4 2306 78-79 0.3183827629777447 2306 80-81 0.5201122977127888 2306 82-83 0.5678393133640789 2306 84-85 0.6344879139837865 2306 86-87 0.5385521405971083 2306 88-89 0.9295074624894042 2306 90-91 0.5785623086281326 2306 92-93 0.5409446693820747 2306 94-95 0.5409899166485772 2306 96-97 0.49645767365160154 2306 98-99 0.3545916586148756 2306 100-101 0.22000396457168137 2307 1 0.16012866893009914 2307 2 0.1435314686210063 2307 3 0.13548123071691265 2307 4 0.14679260504497194 2307 5 0.13868743639469017 2307 6 0.17826660549970086 2307 7 0.20445565928795162 2307 8 0.24002299708758557 2307 9 0.21919612883428385 2307 10-11 0.18178817113516743 2307 12-13 0.19338764090228722 2307 14-15 0.3497392960005783 2307 16-17 0.39696720799756235 2307 18-19 0.31419434047993633 2307 20-21 0.3023383841296621 2307 22-23 0.34057181091183253 2307 24-25 0.32926097733381 2307 26-27 0.36030030533333957 2307 28-29 0.37308194057583677 2307 30-31 0.4398607172588953 2307 32-33 0.4406113899555777 2307 34-35 0.3867511789679554 2307 36-37 0.39203405590416196 2307 38-39 0.4838704167727954 2307 40-41 0.4208823931023602 2307 42-43 0.41060898686095726 2307 44-45 0.43674579091881327 2307 46-47 0.48256783858901287 2307 48-49 0.41736884426639875 2307 50-51 0.43521488112195783 2307 52-53 0.48040219691502983 2307 54-55 0.3314555118267464 2307 56-57 0.40406209001565685 2307 58-59 0.47744565594030064 2307 60-61 0.47360395118926846 2307 62-63 0.49274041858279105 2307 64-65 0.5258909162323775 2307 66-67 0.4826607029346448 2307 68-69 0.2396808278411413 2307 70-71 0.23210671376478587 2307 72-73 -9.823571017832933E-4 2307 74-75 0.06511152003346865 2307 76-77 -0.28532816761606483 2307 78-79 0.05941105624408749 2307 80-81 0.1654236940001219 2307 82-83 0.3232683437379684 2307 84-85 0.3891808360309277 2307 86-87 0.2454943803578864 2307 88-89 0.5390336664295248 2307 90-91 0.30001687740854877 2307 92-93 0.3076753680515587 2307 94-95 0.2642450492301798 2307 96-97 0.34203137731449473 2307 98-99 0.34305506733342384 2307 100-101 0.23628247841896766 2201 1 0.16473426814671654 2201 2 0.1263385857059376 2201 3 0.11306676468572618 2201 4 0.1356874452715502 2201 5 0.15551974004360147 2201 6 0.11721065218605986 2201 7 0.18963067110390597 2201 8 0.1942222732298191 2201 9 0.16237599354276 2201 10-11 0.20307209506643176 2201 12-13 0.2361885361204017 2201 14-15 0.3773444334848861 2201 16-17 0.35717288949987136 2201 18-19 0.2784944029576266 2201 20-21 0.38386835535556685 2201 22-23 0.3537863934735057 2201 24-25 0.373823462448847 2201 26-27 0.37236181811127267 2201 28-29 0.36855404653188373 2201 30-31 0.3518868741832648 2201 32-33 0.5058614458828572 2201 34-35 0.44095466073225964 2201 36-37 0.4142547361407054 2201 38-39 0.48229736416326574 2201 40-41 0.44619625042938793 2201 42-43 0.4718195325707484 2201 44-45 0.5077693411754467 2201 46-47 0.46104613001313055 2201 48-49 0.425216078350779 2201 50-51 0.40101257447798844 2201 52-53 0.4483157203279333 2201 54-55 0.4317578258458923 2201 56-57 0.5128994993033871 2201 58-59 0.5858148234002627 2201 60-61 0.47638920449245603 2201 62-63 0.4094519247694208 2201 64-65 0.5080902289096798 2201 66-67 0.4548684936552547 2201 68-69 0.371141212040051 2201 70-71 0.3143055629375482 2201 72-73 0.14646532107447285 2201 74-75 0.2333269962046387 2201 76-77 0.05659333679970402 2201 78-79 0.32851582721899675 2201 80-81 0.4180199669601272 2201 82-83 0.5068211510145595 2201 84-85 0.5022150403483714 2201 86-87 0.4866379333028483 2201 88-89 0.7979959356605022 2201 90-91 0.4788302144517331 2201 92-93 0.6376105769332874 2201 94-95 0.6397535580147498 2201 96-97 0.5745689119802044 2201 98-99 0.46502461672112716 2201 100-101 0.4620483480986657 1101 1 0.12783846416338918 1101 2 0.09839777219457346 1101 3 0.10005001754912257 1101 4 0.11369863760242538 1101 5 0.1051527826882861 1101 6 0.09688247384785598 1101 7 0.14422559489618436 1101 8 0.12646275966903175 1101 9 0.1638434681025558 1101 10-11 0.10417838182900852 1101 12-13 0.13587880323687074 1101 14-15 0.3067906611422231 1101 16-17 0.3284593957210191 1101 18-19 0.2506605373524735 1101 20-21 0.28803513877981146 1101 22-23 0.2751194171392157 1101 24-25 0.2756525041405382 1101 26-27 0.3237381789285436 1101 28-29 0.27328371367304527 1101 30-31 0.3235131908569926 1101 32-33 0.3559094684295303 1101 34-35 0.31761187911021693 1101 36-37 0.27336677728798975 1101 38-39 0.3880474415903663 1101 40-41 0.34493276615226165 1101 42-43 0.3743009075439332 1101 44-45 0.35184590168184116 1101 46-47 0.5138179774574425 1101 48-49 0.39913954182812716 1101 50-51 0.3971525687977575 1101 52-53 0.28525353868609216 1101 54-55 0.36105271732130007 1101 56-57 0.3715866856104384 1101 58-59 0.4508818377659125 1101 60-61 0.41707209012317037 1101 62-63 0.4454707926316104 1101 64-65 0.3768261744068724 1101 66-67 0.3803726080293117 1101 68-69 0.33739939236181016 1101 70-71 0.17950494675118733 1101 72-73 0.031192058772980147 1101 74-75 -0.08583932895884772 1101 76-77 -0.3988992641550233 1101 78-79 0.08114125147730178 1101 80-81 0.2456833277454642 1101 82-83 0.3606896550152534 1101 84-85 0.3322161122747467 1101 86-87 0.24605733343277691 1101 88-89 0.37597946715133546 1101 90-91 0.30792112788107673 1101 92-93 0.2546217427107429 1101 94-95 0.4222876862051521 1101 96-97 0.3603635441008173 1101 98-99 0.2512735938941155 1101 100-101 0.17640838663417568 2304 1 0.10044654448681456 2304 2 0.12213133295901457 2304 3 0.09386890819083504 2304 4 0.10874222705342618 2304 5 0.10192723213263832 2304 6 0.10046844018760481 2304 7 0.16683896462532033 2304 8 0.14298699114878843 2304 9 0.15475145180830907 2304 10-11 0.16852249525392438 2304 12-13 0.14268098431785603 2304 14-15 0.30526057450639854 2304 16-17 0.2644248579022488 2304 18-19 0.2729391514502879 2304 20-21 0.25965650354038416 2304 22-23 0.34576608553114596 2304 24-25 0.25005490132161157 2304 26-27 0.24412104654610545 2304 28-29 0.2631545684180665 2304 30-31 0.3843328476223107 2304 32-33 0.3211462830987273 2304 34-35 0.33800911321248606 2304 36-37 0.32909737491828395 2304 38-39 0.3102984856945099 2304 40-41 0.31304575852778527 2304 42-43 0.44567008795065277 2304 44-45 0.3791820998832094 2304 46-47 0.443727030087274 2304 48-49 0.45770873384326904 2304 50-51 0.3700980454542986 2304 52-53 0.43677868800977393 2304 54-55 0.42330782223797314 2304 56-57 0.30319128218135205 2304 58-59 0.5198262404303691 2304 60-61 0.49053174609467476 2304 62-63 0.43762540097365843 2304 64-65 0.35072923888528607 2304 66-67 0.3073104443620309 2304 68-69 0.3683956565956592 2304 70-71 0.2860325641974697 2304 72-73 0.053814604274009525 2304 74-75 0.20620881909030686 2304 76-77 0.04871444032974992 2304 78-79 0.3196778716361415 2304 80-81 0.374022581852298 2304 82-83 0.43678107707158276 2304 84-85 0.4570928995297301 2304 86-87 0.46513142122902096 2304 88-89 0.6489281126500295 2304 90-91 0.43211205286129584 2304 92-93 0.5409581594768618 2304 94-95 0.5580451784496248 2304 96-97 0.4072323999679526 2304 98-99 0.43467428112741757 2304 100-101 0.4615476852541782 2202 1 0.04972299025486393 2202 2 0.051566323798432734 2202 3 -0.02470599082048608 2202 4 -0.02405645220745356 2202 5 0.01889732617369333 2202 6 0.041046804075818955 2202 7 0.03413313348713842 2202 8 0.07787778998701356 2202 9 0.14295305632267485 2202 10-11 0.15231141645318758 2202 12-13 0.11756731075353599 2202 14-15 0.03693182473180201 2202 16-17 0.0579254747828557 2202 18-19 0.020459314676990914 2202 20-21 0.1767450915471116 2202 22-23 0.14971015463572002 2202 24-25 0.077238681175956 2202 26-27 0.09801050380887233 2202 28-29 0.18757401700483456 2202 30-31 0.22108238160174665 2202 32-33 0.22924574886603466 2202 34-35 0.19191775036914294 2202 36-37 0.1526874674569143 2202 38-39 0.22417662100779268 2202 40-41 0.14223434585159112 2202 42-43 0.2660762454521617 2202 44-45 0.16629668391126984 2202 46-47 0.2878146171069602 2202 48-49 0.2161926039157578 2202 50-51 0.21357696144738014 2202 52-53 0.16810310182064114 2202 54-55 0.11111857829341432 2202 56-57 0.16208807212097298 2202 58-59 0.17946340333464406 2202 60-61 0.13927494722187106 2202 62-63 0.1704096662536685 2202 64-65 0.2121154192195931 2202 66-67 0.15390420418354722 2202 68-69 0.060009044568438696 2202 70-71 -0.041369750205454636 2202 72-73 -0.2276739501969658 2202 74-75 0.016517343666798467 2202 76-77 -0.2282765016747561 2202 78-79 0.14326869068590398 2202 80-81 0.2653085457879989 2202 82-83 0.3848947417724524 2202 84-85 0.2817113685760688 2202 86-87 0.21439535647537156 2202 88-89 0.42206549430681406 2202 90-91 0.2202896679780757 2202 92-93 0.3188860102375273 2202 94-95 0.2651000519807454 2202 96-97 0.2899494557031268 2202 98-99 0.19152640719686076 2202 100-101 0.24102191495984826 1102 1 0.06991539708297978 1102 2 0.034236851214132 1102 3 0.05131650621266459 1102 4 0.06626032383818625 1102 5 0.04958729012508911 1102 6 -0.023789230084680923 1102 7 0.054241340236401925 1102 8 0.09999820320415864 1102 9 0.0838845082929538 1102 10-11 0.06786551283306608 1102 12-13 0.07227505776160115 1102 14-15 0.13258149170952294 1102 16-17 0.13820299210318154 1102 18-19 0.15620810707464017 1102 20-21 0.14064060205591744 1102 22-23 0.18367534816500353 1102 24-25 0.10291244735645932 1102 26-27 0.13681463640136826 1102 28-29 0.16627676919404877 1102 30-31 0.17163154372544653 1102 32-33 0.19766655314431603 1102 34-35 0.16475984816323574 1102 36-37 0.09218646558561261 1102 38-39 0.2432927140153751 1102 40-41 0.23565038646655267 1102 42-43 0.20178512174992846 1102 44-45 0.09604592686703484 1102 46-47 0.23892197751386846 1102 48-49 0.11376130032619614 1102 50-51 0.08874126671845062 1102 52-53 0.10829392200351862 1102 54-55 0.09264280057302443 1102 56-57 0.03473738243242508 1102 58-59 0.2170723991307426 1102 60-61 0.06441107835718185 1102 62-63 0.11649681622967023 1102 64-65 0.21160603066959283 1102 66-67 0.12752749890719883 1102 68-69 0.12046884541157254 1102 70-71 -0.07773697025520931 1102 72-73 -0.11447426782422099 1102 74-75 -0.06566397251988931 1102 76-77 -0.023347565006414328 1102 78-79 0.10637026461780508 1102 80-81 0.15023056577069482 1102 82-83 0.18953751219361337 1102 84-85 0.1422554922898378 1102 86-87 0.08661143106857239 1102 88-89 0.3378788786899527 1102 90-91 0.1150268955387439 1102 92-93 0.1249860340547464 1102 94-95 0.20939696356132487 1102 96-97 0.08265164629535704 1102 98-99 0.051115188922146615 1102 100-101 0.120898442560879 2305 1 0.07320681860606726 2305 2 0.0834772390559877 2305 3 0.02661120256875904 2305 4 0.045952040660480975 2305 5 0.033913625390262325 2305 6 0.0017122434956036159 2305 7 0.03293010201759472 2305 8 0.03640153881181618 2305 9 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-0.6715575153102726 1213 90-91 -0.7424875485353972 1213 92-93 -0.8993127816522097 1213 94-95 -0.7548359148463035 1213 96-97 -0.7523624543749001 1213 98-99 -0.8400827548954766 1213 100-101 -0.7811066608337178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 10.0 9 32.0 10 60.0 11 28.0 12 54.0 13 156.0 14 401.0 15 979.0 16 1870.0 17 3263.0 18 4713.0 19 6624.0 20 9410.0 21 12742.0 22 17222.0 23 23426.0 24 31633.0 25 42306.0 26 57895.0 27 83124.0 28 135235.0 29 223168.0 30 209405.0 31 128088.0 32 144811.0 33 205960.0 34 320161.0 35 574678.0 36 1169728.0 37 1725365.0 38 320299.0 39 48.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.07307127112417 24.73264144011756 20.603141072740634 14.591146216017634 2 28.08444758813526 29.44565063304882 28.676811610415527 13.793090168400393 3 33.017103236708046 18.174944423925236 31.774403499357955 17.03354884000876 4 27.349900581834724 17.8699199518684 29.017521075917323 25.76265839037955 5 36.86748530184964 16.837086781576836 29.21839528602869 17.077032630544828 6 32.77826447116093 17.723239591725278 32.56230587153948 16.936190065574316 7 29.75425723243993 26.734174513107433 25.39780230582019 18.113765948632444 8 26.8563114249178 32.50862798252012 22.738188526209097 17.896872066352987 9 34.47413365776027 22.971128924679853 23.845288110155025 18.709449307404853 10-11 26.19026965844169 29.939109029186696 26.917502573419718 16.9531187389519 12-13 21.782164599844815 28.777116090767898 26.490093247681003 22.95062606170628 14-15 21.363643949262197 29.12473864809843 26.967875974917554 22.543741427721812 16-17 25.24150922669641 25.2475060956706 27.641852313622312 21.869132364010678 18-19 20.092888239040644 25.38909133988777 32.06494639454954 22.45307402652205 20-21 24.37781238119847 25.819422226387186 31.70674230597057 18.096023086443775 22-23 19.7899250357106 34.65762847883763 27.274855429157125 18.277591056294646 24-25 20.065260722878133 25.146422167886172 31.781253157725146 23.007063951510546 26-27 20.133352968156192 25.214523582602055 27.024488084040467 27.627635365201286 28-29 24.08884671762551 29.957451974610926 26.924614567265802 19.029086740497757 30-31 19.073283430807514 26.45950400310147 31.092270769097603 23.374941796993415 32-33 18.75724041734146 30.801837995472862 27.33366318857664 23.107258398609034 34-35 19.122514142482427 30.761382435795138 32.2767512388369 17.839352182885538 36-37 18.80861329791453 27.009612867405515 32.31764688082694 21.86412695385301 38-39 23.957284477308306 31.003368779222594 26.909805495844342 18.129541247624758 40-41 19.425936259053564 34.807035336698384 26.880502804387067 18.886525599860985 42-43 19.57663292506577 30.73301098627014 31.36324748313455 18.32710860552954 44-45 19.195620343717632 26.479034442185075 31.50326214851395 22.822083065583342 46-47 23.92218484277202 26.27871327271865 27.038547110990923 22.760554773518404 48-49 19.942478979370986 30.976517583911722 30.772691463081266 18.30831197363603 50-51 24.163609407335514 26.42460582078702 30.46197627698806 18.949808494889407 52-53 19.351733767898583 26.27093280047007 26.615489346219174 27.761844085412175 54-55 23.998145733260703 26.450656513134845 30.83127456536611 18.719923188238337 56-57 19.657323640791184 26.063046279678503 30.82097989102906 23.458650188501252 58-59 19.60239086430827 25.994596067015372 31.28519503960775 23.11781802906861 60-61 23.702805737496625 26.514238142737153 30.800459297589423 18.9824968221768 62-63 19.265230970112988 26.649452490156563 30.699741603962682 23.38557493576777 64-65 24.035635167897205 26.377371890318678 30.78516186281728 18.801831078966842 66-67 19.995969503247238 34.83829672711556 26.259998044374356 18.905735725262847 68-69 19.95367378649111 34.879432801035556 26.291832033358688 18.875061379114648 70-71 19.560715877217074 35.446243622293366 26.459545650262356 18.533494850227203 72-73 19.496454994880935 35.68587121603334 26.095554164218427 18.7221196248673 74-75 20.460089668000926 34.42698148304416 25.796005297896617 19.316923551058295 76-77 20.85224712077002 33.05798436334873 26.395918924097998 19.69384959178325 78-79 20.49424021963542 32.55905599910784 27.190603938705166 19.756099842551574 80-81 19.90660599357687 32.67457727840474 27.026174899168588 20.392641828849797 82-83 19.121236531655793 32.579911449691515 27.31390520431354 20.984946814339153 84-85 19.31864607380055 31.897070884060994 27.646286830831123 21.137996211307332 86-87 19.20836370996876 31.91948266369609 28.149820546653785 20.722333079681356 88-89 19.47715748796843 32.41413364897806 27.777349776647608 20.331359086405907 90-91 20.00490508504517 31.43680745367478 27.547849113787425 21.010438347492624 92-93 19.585683322452006 31.917953475008616 27.04131764085968 21.455045561679697 94-95 19.323868631516383 32.22869926159916 26.910452217197435 21.536979889687018 96-97 19.940804424378104 32.510247187251665 26.632074613318192 20.916873775052043 98-99 20.260869246158602 31.493758272845433 26.78533393630127 21.460038544694694 100-101 20.220021790328616 31.568711069046728 26.393113669212543 21.818153471412117 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 74.0 1 48.5 2 36.5 3 80.5 4 102.0 5 79.5 6 63.0 7 65.0 8 65.0 9 65.5 10 61.5 11 63.5 12 68.5 13 73.0 14 89.0 15 93.0 16 104.0 17 122.0 18 159.0 19 202.0 20 245.5 21 332.0 22 413.5 23 539.5 24 736.0 25 1008.0 26 1412.5 27 1919.0 28 2579.5 29 3513.0 30 5130.0 31 8617.5 32 19093.5 33 41110.0 34 77283.0 35 128695.0 36 200680.0 37 296495.0 38 380930.0 39 407834.0 40 410825.0 41 389712.5 42 357675.5 43 368943.5 44 364309.5 45 338481.0 46 339643.5 47 311130.0 48 239877.5 49 186324.0 50 150299.5 51 120431.5 52 91442.0 53 63215.5 54 44408.5 55 31388.0 56 22279.0 57 15179.5 58 9166.5 59 5770.5 60 3883.0 61 2773.5 62 1899.0 63 1061.5 64 642.0 65 482.0 66 364.5 67 296.5 68 233.0 69 160.5 70 108.0 71 66.5 72 45.0 73 34.0 74 30.0 75 22.5 76 16.0 77 13.0 78 6.5 79 2.5 80 2.0 81 1.0 82 0.5 83 1.5 84 2.0 85 0.5 86 0.0 87 0.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16316097663315482 2 0.0 3 2.017276321192203E-4 4 0.02158485663675657 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.601995232992664E-4 12-13 0.0 14-15 0.0 16-17 9.44452095830895E-4 18-19 0.0 20-21 0.0 22-23 6.235217720048627E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.077743445364913E-4 38-39 5.776745828868582E-4 40-41 0.005125715743392915 42-43 0.0033376753677907354 44-45 8.986049067128903E-4 46-47 0.004722260479154475 48-49 0.008353357857300441 50-51 0.005520001569807756 52-53 0.0016688376838953677 54-55 0.01113169751785152 56-57 0.019741799634212787 58-59 0.024060604849128822 60-61 0.012076149613682415 62-63 4.951496424744498E-4 64-65 0.014872828149880698 66-67 0.03587084076592681 68-69 0.020612896227454873 70-71 0.03803482809229663 72-73 0.031286121854126346 74-75 0.030754294460357493 76-77 0.039932901721782016 78-79 0.01747694849178336 80-81 0.013332362595515741 82-83 0.001182857479244519 84-85 0.011379272339088744 86-87 0.02203415909011302 88-89 0.02196997302534781 90-91 0.0258853229760254 92-93 0.044535959509229686 94-95 0.029149642841227334 96-97 0.03016745043964704 98-99 0.038181539097474244 100-101 0.01718352648142813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5452897.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.74275461091585 #Duplication Level Percentage of deduplicated Percentage of total 1 78.71817879517472 16.328318661665087 2 8.293424505750016 3.4405693881385737 3 3.3701400449732555 2.0971796387190347 4 1.7558574504801001 1.4568528090822817 5 1.1615743366454208 1.2047125713686662 6 0.8086841877623648 1.006460259868952 7 0.6376040334387216 0.9257964803184711 8 0.487293360696423 0.8086245283563528 9 0.4725645067470231 0.8822060641153789 >10 3.3000580225538902 13.77706935536981 >50 0.450823440862359 6.534530285044069 >100 0.4327212247474418 19.73696552098 >500 0.07315066178433183 10.590112083205021 >1k 0.037305376951626314 12.56416678998549 >5k 5.314732350170149E-4 0.7456218390598748 >10k+ 8.857819734613988E-5 7.900813724722648 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 430010 7.8858999170532655 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8692 0.15940150712547846 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7726 0.14168615325028144 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7252 0.13299352619350777 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6294 0.11542488332348842 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 5494 0.10075378280572694 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004914818673450095 0.0 0.0 2 0.005226579559452526 0.0 0.0 3 0.005648373699338168 0.0 0.0 4 0.006106845590518215 0.0 0.0 5 0.00685873949205349 0.0 0.0 6 0.007794022150060784 0.0 0.0 7 0.008931032440187298 0.0 0.0 8 0.010196414859844226 0.0 0.0 9 0.011351764025617942 0.0 0.0 10-11 0.01700930716277971 0.0 0.0 12-13 0.02218087009529063 0.0 0.0 14-15 0.02766419391380398 0.0 0.0 16-17 0.03203801575566163 0.0 0.0 18-19 0.037411306320291764 0.0 0.0 20-21 0.04323389933827835 0.0 0.0 22-23 0.049881741760389016 0.0 0.0 24-25 0.057180614267975355 0.0 0.0 26-27 0.06498380585585974 0.0 0.0 28-29 0.07371311066392781 0.0 0.0 30-31 0.11364601238570983 0.0 0.0 32-33 0.12512614854085818 0.0 9.169437823600923E-6 34-35 0.13463485556393234 0.0 1.8338875647201846E-5 36-37 0.14582156970872548 0.0 1.8338875647201846E-5 38-39 0.1571641642965198 0.0 1.8338875647201846E-5 40-41 0.17064323789721317 0.0 1.8338875647201846E-5 42-43 0.18465413889167537 0.0 1.8338875647201846E-5 44-45 0.19890344526955123 0.0 1.8338875647201846E-5 46-47 0.2146382005748504 0.0 1.8338875647201846E-5 48-49 0.2315741522350413 0.0 1.8338875647201846E-5 50-51 0.25001389169830274 0.0 1.8338875647201846E-5 52-53 0.26953562482474913 0.0 1.8338875647201846E-5 54-55 0.290194368241322 0.0 1.8338875647201846E-5 56-57 0.312320221709671 0.0 1.8338875647201846E-5 58-59 0.33518879964173176 0.0 1.8338875647201846E-5 60-61 0.36139505294158314 0.0 1.8338875647201846E-5 62-63 0.38915094123362315 0.0 1.8338875647201846E-5 64-65 0.41922669729503415 0.0 1.8338875647201846E-5 66-67 0.451237204737225 0.0 1.8338875647201846E-5 68-69 0.4856959520783173 0.0 1.8338875647201846E-5 70-71 0.522282008994485 0.0 1.8338875647201846E-5 72-73 0.5621415552136781 0.0 2.750831347080277E-5 74-75 0.605870604194431 0.0 3.667775129440369E-5 76-77 0.6531207172994465 0.0 3.667775129440369E-5 78-79 0.7038368779017832 0.0 4.584718911800461E-5 80-81 0.7587251327138583 0.0 5.501662694160553E-5 82-83 0.8209489377848143 0.0 5.501662694160553E-5 84-85 0.8890228441872274 0.0 5.501662694160553E-5 86-87 0.9604527648330786 0.0 5.501662694160553E-5 88-89 1.0380262088207424 0.0 5.501662694160553E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 57005 0.0 82.24802 1 TCGGAAG 57015 0.0 82.12886 3 CGGAAGA 56845 0.0 82.09875 4 GAAGAGC 56860 0.0 82.030136 6 ATCGGAA 57785 0.0 81.08379 2 GGAAGAG 58165 0.0 80.32849 5 GTCGCGC 20 0.002062328 71.35574 1 CGGAGCG 45 2.0281914E-7 63.427326 1 AAGAGCA 81250 0.0 57.382572 7 GAGCACA 81705 0.0 57.167637 9 AGAGCAC 81870 0.0 57.075623 8 TCGCCGG 150 0.0 56.99455 2 GTCGCCG 75 2.3574103E-9 50.74186 1 TCGGACG 135 0.0 49.254547 3 CGAGTGG 145 0.0 49.210857 1 GCGCGAC 50 2.8037175E-5 47.570496 1 AAGGCCG 60 1.4781417E-6 47.570496 1 CGATCGG 100 1.2732926E-11 47.570496 1 CGGCAGA 250 0.0 47.495453 4 GTCTCCG 50 2.8327611E-5 47.487617 9 >>END_MODULE