FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34042-1.3410000003584a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34042-1.3410000003584a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5452897
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4300107.8858999170532655TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC86920.15940150712547846No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG77260.14168615325028144No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC72520.13299352619350777No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG62940.11542488332348842No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT54940.10075378280572694TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA570050.082.248021
TCGGAAG570150.082.128863
CGGAAGA568450.082.098754
GAAGAGC568600.082.0301366
ATCGGAA577850.081.083792
GGAAGAG581650.080.328495
GTCGCGC200.00206232871.355741
CGGAGCG452.0281914E-763.4273261
AAGAGCA812500.057.3825727
GAGCACA817050.057.1676379
AGAGCAC818700.057.0756238
TCGCCGG1500.056.994552
GTCGCCG752.3574103E-950.741861
TCGGACG1350.049.2545473
CGAGTGG1450.049.2108571
GCGCGAC502.8037175E-547.5704961
AAGGCCG601.4781417E-647.5704961
CGATCGG1001.2732926E-1147.5704961
CGGCAGA2500.047.4954534
GTCTCCG502.8327611E-547.4876179
Produced by FastQC (version 0.11.2)