##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_34012-1.3410000003579b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2889630 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65608261265283 33.0 33.0 33.0 33.0 33.0 2 32.687391119278246 33.0 33.0 33.0 33.0 33.0 3 32.72675982738274 33.0 33.0 33.0 33.0 33.0 4 36.63886725982219 37.0 37.0 37.0 37.0 37.0 5 36.65270605579261 37.0 37.0 37.0 37.0 37.0 6 36.550289137363606 37.0 37.0 37.0 37.0 37.0 7 36.42403525710904 37.0 37.0 37.0 37.0 37.0 8 36.38736793291875 37.0 37.0 37.0 37.0 37.0 9 36.45263407425864 37.0 37.0 37.0 37.0 37.0 10-11 36.37946051916681 37.0 37.0 37.0 37.0 37.0 12-13 36.364741333665556 37.0 37.0 37.0 37.0 37.0 14-15 38.265271332315905 40.0 37.0 40.0 37.0 40.0 16-17 38.28645951211747 40.0 37.0 40.0 37.0 40.0 18-19 38.238361658759075 40.0 37.0 40.0 37.0 40.0 20-21 38.2614826119607 40.0 37.0 40.0 37.0 40.0 22-23 38.098509151690706 40.0 37.0 40.0 37.0 40.0 24-25 38.13340981371317 40.0 37.0 40.0 37.0 40.0 26-27 38.009813021044216 40.0 37.0 40.0 37.0 40.0 28-29 37.85893695732672 40.0 37.0 40.0 33.0 40.0 30-31 37.81717019133937 40.0 37.0 40.0 33.0 40.0 32-33 37.7107183272599 40.0 37.0 40.0 33.0 40.0 34-35 37.68231884358897 40.0 37.0 40.0 33.0 40.0 36-37 37.650306094551894 40.0 37.0 40.0 33.0 40.0 38-39 37.48064077407834 40.0 37.0 40.0 33.0 40.0 40-41 37.40482276277586 40.0 37.0 40.0 33.0 40.0 42-43 37.15064229676464 40.0 37.0 40.0 33.0 40.0 44-45 37.14322387295259 40.0 37.0 40.0 33.0 40.0 46-47 37.084478289608015 40.0 37.0 40.0 33.0 40.0 48-49 36.82939390164139 37.0 37.0 40.0 33.0 40.0 50-51 36.80699605139758 37.0 37.0 40.0 33.0 40.0 52-53 36.74746628461083 37.0 37.0 40.0 33.0 40.0 54-55 36.45725871478355 37.0 37.0 40.0 33.0 40.0 56-57 36.332897464381254 37.0 37.0 40.0 33.0 40.0 58-59 35.99453615168724 37.0 37.0 40.0 33.0 40.0 60-61 35.741998802614866 37.0 37.0 40.0 30.0 40.0 62-63 35.53780068728523 37.0 35.0 40.0 27.0 40.0 64-65 35.27826417222966 37.0 33.0 37.0 27.0 40.0 66-67 34.63738523617211 37.0 33.0 37.0 27.0 40.0 68-69 34.38620619248831 37.0 33.0 37.0 27.0 40.0 70-71 33.92033305302063 37.0 33.0 37.0 27.0 40.0 72-73 32.931417690154106 37.0 33.0 37.0 22.0 38.5 74-75 31.9026065620858 37.0 33.0 37.0 15.0 37.0 76-77 29.40288358717206 33.0 27.0 35.0 15.0 37.0 78-79 31.4782695362382 35.0 33.0 37.0 15.0 37.0 80-81 31.673536231282206 37.0 33.0 37.0 15.0 37.0 82-83 31.502933766606795 37.0 33.0 37.0 15.0 37.0 84-85 31.33760948633562 37.0 33.0 37.0 15.0 37.0 86-87 31.218733194215176 37.0 33.0 37.0 15.0 37.0 88-89 30.95125829258417 33.0 33.0 37.0 15.0 37.0 90-91 30.90977460782176 33.0 33.0 37.0 15.0 37.0 92-93 30.756192488311655 33.0 33.0 37.0 15.0 37.0 94-95 30.58090810934272 33.0 33.0 37.0 15.0 37.0 96-97 30.472988583313434 33.0 33.0 37.0 15.0 37.0 98-99 30.365055733779066 33.0 27.0 37.0 15.0 37.0 100-101 28.896198994334917 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.21011641729351282 2306 2 0.15785184603106472 2306 3 0.1310791003314975 2306 4 0.1879003638920551 2306 5 0.16706247572783184 2306 6 0.12407572555169821 2306 7 0.2721248648236667 2306 8 0.24213008928737167 2306 9 0.23653700147371381 2306 10-11 0.25476582459832287 2306 12-13 0.2259928017567745 2306 14-15 0.4610004305360462 2306 16-17 0.45788297460696015 2306 18-19 0.40032688483188394 2306 20-21 0.4252072133115803 2306 22-23 0.44282616502258065 2306 24-25 0.48380658644816066 2306 26-27 0.32933557960935644 2306 28-29 0.37057195216776506 2306 30-31 0.40825873701809456 2306 32-33 0.4115664330826263 2306 34-35 0.505512532883678 2306 36-37 0.4268169353181577 2306 38-39 0.5060817937820872 2306 40-41 0.4792271537731665 2306 42-43 0.3856677377556963 2306 44-45 0.3672733573753959 2306 46-47 0.5031137594395148 2306 48-49 0.5116089407571636 2306 50-51 0.34158051791226995 2306 52-53 0.36691816986726167 2306 54-55 0.44818816448807297 2306 56-57 0.47521250791906056 2306 58-59 0.5265792309277302 2306 60-61 0.3943632727305655 2306 62-63 0.44845150612189144 2306 64-65 0.40374127604068377 2306 66-67 0.4709554445559192 2306 68-69 0.4120851065846125 2306 70-71 0.3697652368636142 2306 72-73 0.26799516630781284 2306 74-75 0.3104838232529943 2306 76-77 0.17219653732373175 2306 78-79 0.48365582726661316 2306 80-81 0.5591856084873648 2306 82-83 0.5746649903308025 2306 84-85 0.6782311696439187 2306 86-87 0.602369980054366 2306 88-89 0.8928508086698841 2306 90-91 0.5997246924574888 2306 92-93 0.5963540053406113 2306 94-95 0.5949959714533435 2306 96-97 0.5719141762375806 2306 98-99 0.5796171351920911 2306 100-101 0.5214389202477321 2307 1 0.10061318696768495 2307 2 0.07554640815540381 2307 3 0.13140309598046684 2307 4 0.19182460405838953 2307 5 0.13610284643724668 2307 6 0.14406674369485017 2307 7 0.1498980131266947 2307 8 0.21361806667660943 2307 9 0.14564202938741744 2307 10-11 0.1862858568762391 2307 12-13 0.14227581337540585 2307 14-15 0.41602596192033303 2307 16-17 0.38639408483948756 2307 18-19 0.33748815174002544 2307 20-21 0.36540913275263875 2307 22-23 0.3938567976149585 2307 24-25 0.3110781801600524 2307 26-27 0.43928040100117727 2307 28-29 0.35934655723470144 2307 30-31 0.4159327503570651 2307 32-33 0.35689652645947234 2307 34-35 0.3501653374836806 2307 36-37 0.31185048549338745 2307 38-39 0.37234407413969706 2307 40-41 0.4302516024686227 2307 42-43 0.3651069900921442 2307 44-45 0.27026930337476784 2307 46-47 0.40126315225180065 2307 48-49 0.22580443097292147 2307 50-51 0.35496368737233297 2307 52-53 0.3038083590640994 2307 54-55 0.23155267074444907 2307 56-57 0.32555571419865004 2307 58-59 0.27208450092579284 2307 60-61 0.2224492147053425 2307 62-63 0.25299585936230073 2307 64-65 0.10155795492308073 2307 66-67 0.10174699350044847 2307 68-69 0.034529813929161435 2307 70-71 -0.016135072635357517 2307 72-73 -0.08410842308953903 2307 74-75 -0.08568155239846575 2307 76-77 -0.23411904619790747 2307 78-79 0.10577988434905095 2307 80-81 0.13738868969003803 2307 82-83 0.27608353573631916 2307 84-85 0.293774850765697 2307 86-87 0.2636195530106633 2307 88-89 0.45311896677084107 2307 90-91 0.18310856140753273 2307 92-93 0.26423504743875625 2307 94-95 0.2924930138272295 2307 96-97 0.34080309215989857 2307 98-99 0.2302383127074421 2307 100-101 0.21864420433697163 2201 1 0.17195421629313046 2201 2 0.1692648573258353 2201 3 0.12701519267939432 2201 4 0.19352801141300802 2201 5 0.1507397318203374 2201 6 0.16159521801724708 2201 7 0.1903212541281789 2201 8 0.2945437993500235 2201 9 0.17850946133403767 2201 10-11 0.19987050160570163 2201 12-13 0.2746141169405689 2201 14-15 0.46939087597096574 2201 16-17 0.4236640747123701 2201 18-19 0.36841076066438205 2201 20-21 0.46469036195174596 2201 22-23 0.34514867995528675 2201 24-25 0.31967311783547103 2201 26-27 0.4168422415912474 2201 28-29 0.43513208370983136 2201 30-31 0.4924643129488899 2201 32-33 0.5270819421764799 2201 34-35 0.555602839749362 2201 36-37 0.5258141836728925 2201 38-39 0.5693235437811026 2201 40-41 0.489118074922942 2201 42-43 0.5588479803900128 2201 44-45 0.6417001308443204 2201 46-47 0.7013790780500315 2201 48-49 0.6549905825063718 2201 50-51 0.5792666092732048 2201 52-53 0.6269645372612587 2201 54-55 0.623297026207041 2201 56-57 0.666691092854883 2201 58-59 0.6724346886206902 2201 60-61 0.6610662924342492 2201 62-63 0.658224880302221 2201 64-65 0.5799705350703803 2201 66-67 0.6290086456628998 2201 68-69 0.48245537460354626 2201 70-71 0.36259674695232746 2201 72-73 0.30397391507904814 2201 74-75 0.43368617379759655 2201 76-77 0.26292197286930374 2201 78-79 0.45313190927116764 2201 80-81 0.6589316636071239 2201 82-83 0.7385755046160654 2201 84-85 0.5112393653620906 2201 86-87 0.48957285566184083 2201 88-89 0.834085713112394 2201 90-91 0.47368384414538767 2201 92-93 0.6254654844782443 2201 94-95 0.6554101789439777 2201 96-97 0.5051760207479106 2201 98-99 0.4789711696883252 2201 100-101 0.4348303921213379 1101 1 0.13835498202066532 1101 2 0.10467671224901665 1101 3 0.09978582635671529 1101 4 0.1912900915275344 1101 5 0.09751733009185415 1101 6 0.07697616816589203 1101 7 0.10210271112352132 1101 8 0.09366608836039347 1101 9 0.15661741324377232 1101 10-11 0.12480397972572632 1101 12-13 0.24046747214116948 1101 14-15 0.20526883884465263 1101 16-17 0.24359443576717865 1101 18-19 0.24558002465217754 1101 20-21 0.24411584240309736 1101 22-23 0.294945388530337 1101 24-25 0.2940761001427177 1101 26-27 0.26690720668781154 1101 28-29 0.3157288075378517 1101 30-31 0.40089614927463657 1101 32-33 0.3318809806790597 1101 34-35 0.2148975538924489 1101 36-37 0.32348201716913394 1101 38-39 0.44943416636152733 1101 40-41 0.4004209522085205 1101 42-43 0.4265639832139101 1101 44-45 0.3650451126491987 1101 46-47 0.4176502284395056 1101 48-49 0.5029355234500557 1101 50-51 0.42043132115632886 1101 52-53 0.2505725280031186 1101 54-55 0.4867898604554384 1101 56-57 0.4471650406966958 1101 58-59 0.46243833744113516 1101 60-61 0.3053362974509355 1101 62-63 0.46012620262355597 1101 64-65 0.4204824474371165 1101 66-67 0.40305062913790124 1101 68-69 0.4560789156875842 1101 70-71 0.14785229382409426 1101 72-73 -0.11736435541504164 1101 74-75 -0.04760889858058448 1101 76-77 -0.06627815881008203 1101 78-79 0.18768984225461693 1101 80-81 0.30482002369497607 1101 82-83 0.4495498306337993 1101 84-85 0.4059237546377048 1101 86-87 0.4835183675427217 1101 88-89 0.5677068647962713 1101 90-91 0.4135233485215224 1101 92-93 0.40738855278990016 1101 94-95 0.3986728051571298 1101 96-97 0.40304936714656137 1101 98-99 0.15603333047571866 1101 100-101 0.1732348045702885 2304 1 0.11278838244324163 2304 2 0.07655770233669301 2304 3 0.12254627899427817 2304 4 0.045887074512442894 2304 5 0.11219202223847446 2304 6 0.10444811871641235 2304 7 0.12600172147321587 2304 8 0.11216807769569925 2304 9 0.1304291900033263 2304 10-11 0.08526620220712999 2304 12-13 0.15628236601459378 2304 14-15 0.2548070335707422 2304 16-17 0.20618154721942972 2304 18-19 0.24722659840926298 2304 20-21 0.2874708358882998 2304 22-23 0.2687966371517234 2304 24-25 0.25577637424338917 2304 26-27 0.20970123444778466 2304 28-29 0.2405210296733884 2304 30-31 0.19592497459047564 2304 32-33 0.17847696298635896 2304 34-35 0.34242718454283505 2304 36-37 0.2470503789441807 2304 38-39 0.43919497928646223 2304 40-41 0.35196563582952933 2304 42-43 0.40706612423283417 2304 44-45 0.3808598359173274 2304 46-47 0.30572175424487824 2304 48-49 0.34800544137805645 2304 50-51 0.41530734616458176 2304 52-53 0.22825006569098605 2304 54-55 0.41709813957376696 2304 56-57 0.3230378224982573 2304 58-59 0.47771061341351384 2304 60-61 0.3700962844911331 2304 62-63 0.29570581318129285 2304 64-65 0.358199028922634 2304 66-67 0.3451136132013559 2304 68-69 0.5066089475706193 2304 70-71 0.369764186734848 2304 72-73 0.24320040084969463 2304 74-75 0.38023390135632695 2304 76-77 0.23541962755517076 2304 78-79 0.5076843487640623 2304 80-81 0.6501214219914964 2304 82-83 0.7233077426483021 2304 84-85 0.6567594491741993 2304 86-87 0.6224336528622416 2304 88-89 0.7414751837338791 2304 90-91 0.6832697871747264 2304 92-93 0.6236990085216298 2304 94-95 0.4402082156047804 2304 96-97 0.5017503473980689 2304 98-99 0.5239999526350836 2304 100-101 0.7070462928517962 2202 1 0.06586608034452723 2202 2 0.06639377195826057 2202 3 0.06840357466954572 2202 4 0.09627607600628352 2202 5 0.054012968986697274 2202 6 0.07332625627326905 2202 7 0.11380233251747285 2202 8 0.058460381108879744 2202 9 0.050798663353475604 2202 10-11 0.15572176932577264 2202 12-13 0.06176106697545691 2202 14-15 0.08010080903849115 2202 16-17 0.008050264934261975 2202 18-19 0.04314072105530187 2202 20-21 0.10100458747782426 2202 22-23 0.13108139365269267 2202 24-25 0.13336069421155372 2202 26-27 0.1339357406559003 2202 28-29 0.1579388411705196 2202 30-31 0.18102723045780778 2202 32-33 0.16801927529576943 2202 34-35 0.12855080081516945 2202 36-37 0.10989585243085997 2202 38-39 0.1026653678957885 2202 40-41 0.1297824198659825 2202 42-43 0.19266206667818864 2202 44-45 0.07130382703706317 2202 46-47 0.21507943155047116 2202 48-49 0.06851434959577318 2202 50-51 0.06758307571032418 2202 52-53 0.0779296196737107 2202 54-55 -0.008500649666800086 2202 56-57 0.22134114707575492 2202 58-59 0.23591217958660593 2202 60-61 0.08779136803223508 2202 62-63 0.20392644271519345 2202 64-65 0.14247426173165678 2202 66-67 0.10005879423791697 2202 68-69 0.10807875084399399 2202 70-71 -0.014901754192706562 2202 72-73 -0.11910345532323419 2202 74-75 -0.0929524104970163 2202 76-77 -0.5384549260898517 2202 78-79 -0.06993047258138674 2202 80-81 0.04631695615412568 2202 82-83 0.28412669010387503 2202 84-85 0.29259057902509156 2202 86-87 0.18381905446734237 2202 88-89 0.3490900704394164 2202 90-91 0.286503804634588 2202 92-93 0.33873379098507783 2202 94-95 0.30074800283588843 2202 96-97 0.21636689788111596 2202 98-99 0.24403187740997012 2202 100-101 0.30474648900470314 1102 1 0.08531339598706467 1102 2 0.07633276398811972 1102 3 0.07258310524503742 1102 4 0.05821336542508959 1102 5 0.04850347686229384 1102 6 -0.06887755413231389 1102 7 0.11005699487248677 1102 8 0.1531575474679414 1102 9 0.11376653402552961 1102 10-11 0.03383989610116345 1102 12-13 0.0779760649996959 1102 14-15 0.1676003091676037 1102 16-17 0.11184284461368321 1102 18-19 0.07171456062623349 1102 20-21 0.06243882039799331 1102 22-23 0.06777930422630618 1102 24-25 0.0740945792215939 1102 26-27 0.026230883402078575 1102 28-29 0.11718821472216234 1102 30-31 0.1500027018784138 1102 32-33 0.12833210296536635 1102 34-35 0.06835075757210518 1102 36-37 0.0718731315201282 1102 38-39 0.1671186853778508 1102 40-41 0.08356872997842402 1102 42-43 0.19226508447494695 1102 44-45 0.265685685011384 1102 46-47 0.2233206248422377 1102 48-49 0.07213226252809335 1102 50-51 0.18928411428951364 1102 52-53 0.09781289050341258 1102 54-55 0.05985423629147135 1102 56-57 0.12628942823160827 1102 58-59 0.1956452248135676 1102 60-61 0.24672428095438192 1102 62-63 0.2318094098945167 1102 64-65 0.0516194926923319 1102 66-67 0.07372756183083595 1102 68-69 0.13914577692064967 1102 70-71 -0.02137364179326795 1102 72-73 -0.19403284644322838 1102 74-75 -0.07678662010118131 1102 76-77 0.010615856304664106 1102 78-79 0.16073320005639502 1102 80-81 0.30740016110254587 1102 82-83 0.38323959721058287 1102 84-85 0.24124818001087434 1102 86-87 0.23488030576446306 1102 88-89 0.4072479971037808 1102 90-91 0.19293394030801636 1102 92-93 0.25207461616853877 1102 94-95 0.37623033587393095 1102 96-97 0.3802261993090177 1102 98-99 0.2863068558731605 1102 100-101 0.31194449413015946 2305 1 0.0517399418327571 2305 2 0.09410071774916418 2305 3 0.07066531529547859 2305 4 0.0845987462796316 2305 5 0.035009152848722636 2305 6 -0.036810712776528476 2305 7 0.017325536971426914 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-1.276691115666086 1213 96-97 -1.264598526007621 1213 98-99 -1.2583505833004587 1213 100-101 -1.1879693853589757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 21.0 10 20.0 11 29.0 12 37.0 13 72.0 14 244.0 15 543.0 16 1191.0 17 1904.0 18 2979.0 19 4090.0 20 5701.0 21 7635.0 22 10529.0 23 13764.0 24 18462.0 25 24920.0 26 33988.0 27 50850.0 28 88356.0 29 138587.0 30 98839.0 31 60608.0 32 73810.0 33 102928.0 34 158277.0 35 274316.0 36 551976.0 37 897370.0 38 267368.0 39 209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.05362726162233 22.896928672150903 21.43911904705783 13.610325019168934 2 27.671085917574224 30.076376560320874 29.83648425576977 12.416053266335137 3 34.177780284833005 17.265781730004225 33.364629226719195 15.191808758443576 4 26.961380544965806 17.46819555966608 30.20056336624136 25.369860529126754 5 38.27912224056367 16.179510871634083 30.21701740361223 15.324349484190018 6 33.12562508002755 17.067859899018213 34.34896509241668 15.457549928537565 7 29.439478410730786 27.535809082823754 26.179753117181093 16.844959389264368 8 26.507511342282577 34.52898814035015 22.781428764236253 16.182071753131027 9 36.360087623675 22.986437710018237 23.344165169935252 17.309309496371508 10-11 26.48656430473807 30.801013006572497 26.927868616452844 15.784554072236586 12-13 21.257860002837734 29.209102895526417 26.83404103639566 22.698996065240188 14-15 20.51638098995373 29.81319407675031 27.455850749057838 22.21457418423812 16-17 25.023550037513946 25.152426363306652 28.20872289236681 21.615300706812587 18-19 19.20216429092998 25.288081865152286 33.52406709509522 21.985686748822513 20-21 24.328737589241527 25.787228814761754 33.114360662091684 16.769672933905035 22-23 18.910162961127526 36.30827354019938 27.847147829875635 16.93441566879746 24-25 19.32325245792714 25.29877527572734 33.0753764322768 22.302595834068722 26-27 19.354450223731064 25.615459418679897 27.71140249789765 27.31868785969138 28-29 24.24580309589809 31.054529472631444 27.539511979042295 17.160155452428167 30-31 18.653806888771225 26.429837037959874 32.446714631284976 22.469641441983924 32-33 18.21721120004983 31.802531812031297 27.646255749005928 22.33400123891294 34-35 23.65169589186159 26.445046597661293 28.032412454189636 21.870845056287482 36-37 18.357168268505355 26.456535792546777 33.40747287856157 21.778823060386294 38-39 18.95661316115779 31.505767735357963 27.514483793482093 22.023135310002147 40-41 18.941421802784898 26.07242930776979 37.583793205662126 17.402355683783185 42-43 18.609507909761135 31.231175578320496 32.577414812299985 17.58190169961838 44-45 18.33937079472141 26.15381554005359 32.887452318186185 22.61936134703882 46-47 23.73794599473403 26.206507986167832 27.517809334222992 22.53773668487515 48-49 18.875927045591485 31.675740572246134 32.02264398299867 17.425688399163707 50-51 24.14350239196033 26.420614707674865 31.755172486590105 17.6807104137747 52-53 18.53896810644865 26.25193756465038 26.842819079998105 28.366275248902866 54-55 23.864489820781237 26.43606553518858 31.89891178471875 17.800532859311435 56-57 18.76542778278997 26.29173689554633 31.915683462351407 23.02715185931229 58-59 18.66749327000195 26.248948140332878 32.45800955439918 22.625549035265983 60-61 23.494612863367816 26.506979223269383 32.323719668153764 17.67468824520903 62-63 18.3011611605584 26.591573462015106 31.96653941761671 23.14072595980979 64-65 23.53554106715095 26.425070962378257 31.901885188565583 18.13750278190521 66-67 18.558118965008408 36.54219268872966 26.637904399069107 18.26178394719282 68-69 18.502198907356522 36.88785425374479 26.497132187292806 18.112814651605884 70-71 18.098761841952665 37.25245926610618 26.688217159731565 17.960561732209595 72-73 18.098824935278753 36.68461051024246 26.66588085544033 18.550683699038455 74-75 18.631675456240593 35.76401443602922 26.50339966030225 19.10091044742794 76-77 18.90990554965861 34.83227638409113 26.884523535036497 19.37329453121376 78-79 19.114428604607134 34.25414718415134 27.104241451316298 19.527182759925225 80-81 18.799513230582438 34.20110665282687 27.03198716069517 19.967392955895523 82-83 18.526695991366278 33.695522962430275 27.178480208596312 20.59930083760714 84-85 18.698220715209565 32.873349907088446 27.705674751765734 20.722754625936254 86-87 18.771897821588613 32.722207722709626 27.994078199063615 20.511816256638145 88-89 19.101002251648065 33.135432910060416 27.508129989840757 20.25543484845076 90-91 19.583612926072075 32.489988748213186 27.183718088134324 20.742680237580412 92-93 19.24523768431399 32.53550996328564 27.391033622832456 20.82821872956791 94-95 18.87960991259831 32.593992256615536 27.384091981811753 21.142305848974406 96-97 19.051895058870365 33.26221868360587 27.016031951397874 20.66985430612589 98-99 19.4197054889641 32.51527020213079 26.972836375891646 21.092187933013463 100-101 19.603612933416933 32.38338449365473 26.505453822365666 21.507548750562673 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 153.0 1 106.0 2 90.0 3 188.0 4 206.0 5 131.0 6 101.5 7 111.0 8 116.0 9 108.5 10 110.0 11 112.5 12 123.0 13 133.5 14 137.5 15 152.5 16 178.0 17 206.5 18 250.5 19 356.5 20 505.0 21 718.0 22 1120.0 23 1806.0 24 2865.0 25 4466.0 26 7148.5 27 11298.0 28 15934.0 29 20310.0 30 25127.5 31 29858.5 32 36401.5 33 47822.5 34 64401.5 35 88886.5 36 117956.0 37 151429.5 38 189574.5 39 211580.5 40 214008.5 41 203956.0 42 189559.5 43 182995.5 44 170200.5 45 153620.5 46 147424.0 47 131995.0 48 102066.0 49 78140.5 50 63521.5 51 50857.0 52 40067.0 53 32217.0 54 26119.0 55 20322.0 56 14397.0 57 10043.0 58 7176.5 59 5304.5 60 3921.0 61 2845.5 62 2000.5 63 1356.5 64 943.5 65 674.0 66 484.0 67 338.5 68 237.5 69 172.0 70 127.5 71 95.5 72 63.0 73 42.0 74 27.0 75 23.5 76 22.0 77 17.0 78 16.0 79 10.5 80 5.0 81 2.5 82 3.0 83 2.5 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16451933292497656 2 0.0 3 2.422455470077484E-4 4 0.019656495814343013 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.844910940154968E-4 12-13 0.0 14-15 0.0 16-17 0.00131504725518492 18-19 0.0 20-21 0.0 22-23 5.017943473731931E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0011766212283233493 38-39 7.267366410232452E-4 40-41 0.00520827926066659 42-43 0.00342604416482387 44-45 0.0011247114682502604 46-47 0.0046372718998626125 48-49 0.007232759903517059 50-51 0.005502434567747428 52-53 0.0016957188290542388 54-55 0.0107972300952025 56-57 0.019535373040839136 58-59 0.02418994819405945 60-61 0.011766212283233494 62-63 7.267366410232452E-4 64-65 0.014638552340611082 66-67 0.034866055515758075 68-69 0.020123683655000814 70-71 0.03685592965189315 72-73 0.0309901267636341 74-75 0.030644061696480172 76-77 0.03903613957496289 78-79 0.017337859864411706 80-81 0.01304665303170302 82-83 0.0010208919481040826 84-85 0.01117790166907182 86-87 0.022286590324712852 88-89 0.02197513176427432 90-91 0.026145215823479128 92-93 0.04327543664759848 94-95 0.029121375401002896 96-97 0.030021144575603103 98-99 0.037357723999266346 100-101 0.017632015171492543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2889630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.348877809762115 #Duplication Level Percentage of deduplicated Percentage of total 1 90.62601254891254 45.62918032211165 2 4.398165132197434 4.428853576563297 3 1.349285766888171 2.0380507262251104 4 0.7218624569917235 1.4537985857012377 5 0.49231321063459904 1.2393708843186553 6 0.3835584171436754 1.1587041526603608 7 0.21464641854740343 0.7565044409822389 8 0.2319449991493462 0.9342536336604645 9 0.16521737810701964 0.7486658624123637 >10 1.1813457850373106 11.484725776767464 >50 0.12799849077843198 4.375058335627542 >100 0.09324017501422294 10.249562736022705 >500 0.011547709640307212 3.8459269773792015 >1k 0.0027924894907958627 2.199262247503804 >5k 0.0 0.0 >10k+ 6.902146679284483E-5 9.458081742063932 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 272163 9.418610687181404 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3361 0.11631246907043463 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2923 0.10115481912909266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004152780805847116 0.0 0.0 2 0.004775697926724182 0.0 0.0 3 0.005467828061032035 0.0 0.0 4 0.005917712648332139 0.0 0.0 5 0.0071635468900862745 0.0 0.0 6 0.007924890037824912 0.0 3.460650671539263E-5 7 0.009447576333302187 0.0 3.460650671539263E-5 8 0.010762623588487107 0.0 3.460650671539263E-5 9 0.011973851323525849 0.0 3.460650671539263E-5 10-11 0.01934503725390448 0.0 3.460650671539263E-5 12-13 0.024587923021286463 0.0 3.460650671539263E-5 14-15 0.03010766084239159 0.0 3.460650671539263E-5 16-17 0.0347449327422542 0.0 3.460650671539263E-5 18-19 0.03953793392233608 0.0 3.460650671539263E-5 20-21 0.04519609777030277 0.0 3.460650671539263E-5 22-23 0.05100999089848873 0.0 3.460650671539263E-5 24-25 0.05654703197295155 0.0 3.460650671539263E-5 26-27 0.06326069427573772 0.0 3.460650671539263E-5 28-29 0.07141052660721268 0.0 3.460650671539263E-5 30-31 0.1394296155563169 0.0 3.460650671539263E-5 32-33 0.15154189290670433 0.0 3.460650671539263E-5 34-35 0.15988206102511393 0.0 3.460650671539263E-5 36-37 0.16920851458491226 0.0 3.460650671539263E-5 38-39 0.17910597550551455 0.0 3.460650671539263E-5 40-41 0.19030118042794408 0.0 3.460650671539263E-5 42-43 0.2019116634309583 0.0 3.460650671539263E-5 44-45 0.21561584009025378 0.0 3.460650671539263E-5 46-47 0.2300640566439302 0.0 3.460650671539263E-5 48-49 0.24570619767928764 0.0 3.460650671539263E-5 50-51 0.262040468848953 0.0 3.460650671539263E-5 52-53 0.2798974263140956 0.0 3.460650671539263E-5 54-55 0.298048539086319 0.0 3.460650671539263E-5 56-57 0.3187432301021238 0.0 3.460650671539263E-5 58-59 0.341237459467129 0.0 6.921301343078525E-5 60-61 0.3652024653675384 0.0 6.921301343078525E-5 62-63 0.39169374625817144 0.0 6.921301343078525E-5 64-65 0.4185656987226738 0.0 1.0381952014617788E-4 66-67 0.44817156521769225 0.0 1.0381952014617788E-4 68-69 0.48141111491782684 0.0 1.0381952014617788E-4 70-71 0.5181286185428584 0.0 1.0381952014617788E-4 72-73 0.5572858808913252 0.0 1.0381952014617788E-4 74-75 0.5987444759363656 0.0 1.0381952014617788E-4 76-77 0.6420372158373218 0.0 1.2112277350387419E-4 78-79 0.6909708163328869 0.0 1.384260268615705E-4 80-81 0.7451819091025494 0.0 1.384260268615705E-4 82-83 0.8041340932922207 0.0 1.384260268615705E-4 84-85 0.8691078096503704 0.0 1.384260268615705E-4 86-87 0.9383554295878711 0.0 1.384260268615705E-4 88-89 1.0137630077207116 0.0 1.384260268615705E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 34995 0.0 84.01576 1 TCGGAAG 35025 0.0 83.786896 3 CGGAAGA 35180 0.0 83.32322 4 ATCGGAA 35430 0.0 82.85595 2 GAAGAGC 35575 0.0 82.39572 6 GGAAGAG 37235 0.0 78.83717 5 AGAGCAC 42985 0.0 68.23607 8 GAGCACA 43000 0.0 68.201225 9 AAGAGCA 43965 0.0 66.59624 7 TCGGACG 145 0.0 45.86341 3 GCCGTCT 32035 0.0 45.749313 50-51 ATGCCGT 32010 0.0 45.676826 48-49 CGTCTTC 32105 0.0 45.584568 52-53 TCGTATG 32170 0.0 45.523075 44-45 TCTCGTA 31975 0.0 45.36252 42-43 TATGCCG 32000 0.0 45.357155 48-49 CGTATGC 31995 0.0 45.28221 46-47 TGCCGTC 32105 0.0 45.2797 50-51 GTATGCC 32340 0.0 45.247063 46-47 CCGTCTT 32105 0.0 45.148106 52-53 >>END_MODULE