FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_34012-1.3410000003579b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_34012-1.3410000003579b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889630
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2721639.418610687181404TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC33610.11631246907043463No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG29230.10115481912909266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA349950.084.015761
TCGGAAG350250.083.7868963
CGGAAGA351800.083.323224
ATCGGAA354300.082.855952
GAAGAGC355750.082.395726
GGAAGAG372350.078.837175
AGAGCAC429850.068.236078
GAGCACA430000.068.2012259
AAGAGCA439650.066.596247
TCGGACG1450.045.863413
GCCGTCT320350.045.74931350-51
ATGCCGT320100.045.67682648-49
CGTCTTC321050.045.58456852-53
TCGTATG321700.045.52307544-45
TCTCGTA319750.045.3625242-43
TATGCCG320000.045.35715548-49
CGTATGC319950.045.2822146-47
TGCCGTC321050.045.279750-51
GTATGCC323400.045.24706346-47
CCGTCTT321050.045.14810652-53
Produced by FastQC (version 0.11.2)