FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_30749-1.34100000035870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_30749-1.34100000035870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4991489
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT122402124.522161623515547TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT163460.32747743208489494TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT97510.19535252907499143TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT78760.1577885877340409TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT69930.14009847562520922TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT67080.13438875654138474TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG65140.1305021407439744TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT50340.10085166971218408TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1456850.091.641191
TCGGAAG1464100.090.968723
ATCGGAA1467050.090.81172
CGGAAGA1467150.090.420274
GAAGAGC1473800.089.8693546
GAGCACA1484350.089.019459
GGAAGAG1502000.088.378095
AGAGCAC1503050.088.019358
AAGAGCA1506800.087.72157
GCGCACA9000.048.5452739
TTGTGTT251100.048.4336851
GCCGTCT1419250.046.59091650-51
ATGCCGT1417650.046.5223948-49
GTATGCC1421150.046.50069446-47
TCGTATG1426150.046.3512144-45
TCTCGTA1399750.046.34953742-43
CGTCTTC1427850.046.1862352-53
AATCTCG1393050.046.18239640-41
GTCACAT1426650.046.11802328-29
CAGTCAC1426400.046.0878226-27
Produced by FastQC (version 0.11.2)