##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_30521-1.341000000356df.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3128253 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.679354898724625 33.0 33.0 33.0 33.0 33.0 2 32.71343781976714 33.0 33.0 33.0 33.0 33.0 3 32.74593806830841 33.0 33.0 33.0 33.0 33.0 4 36.667007431943645 37.0 37.0 37.0 37.0 37.0 5 36.669953804887264 37.0 37.0 37.0 37.0 37.0 6 36.56228828039164 37.0 37.0 37.0 37.0 37.0 7 36.498009911602416 37.0 37.0 37.0 37.0 37.0 8 36.43414007754488 37.0 37.0 37.0 37.0 37.0 9 36.44103625889594 37.0 37.0 37.0 37.0 37.0 10-11 36.38723210686604 37.0 37.0 37.0 37.0 37.0 12-13 36.38099699736563 37.0 37.0 37.0 37.0 37.0 14-15 38.28162300172013 40.0 37.0 40.0 37.0 40.0 16-17 38.26264275939318 40.0 37.0 40.0 37.0 40.0 18-19 38.24545904695049 40.0 37.0 40.0 37.0 40.0 20-21 38.19432987037813 40.0 37.0 40.0 37.0 40.0 22-23 38.067638391140356 40.0 37.0 40.0 37.0 40.0 24-25 38.07064917703268 40.0 37.0 40.0 37.0 40.0 26-27 37.98043156995294 40.0 37.0 40.0 35.0 40.0 28-29 37.84452296537397 40.0 37.0 40.0 33.0 40.0 30-31 37.76392942003092 40.0 37.0 40.0 33.0 40.0 32-33 37.65846896015124 40.0 37.0 40.0 33.0 40.0 34-35 37.54809777214311 40.0 37.0 40.0 33.0 40.0 36-37 37.46917912329981 40.0 37.0 40.0 33.0 40.0 38-39 37.38179472696102 40.0 37.0 40.0 33.0 40.0 40-41 37.28792763884507 40.0 37.0 40.0 33.0 40.0 42-43 37.05388998268363 38.5 37.0 40.0 33.0 40.0 44-45 36.931899210198154 37.0 37.0 40.0 33.0 40.0 46-47 36.800956636180004 37.0 37.0 40.0 33.0 40.0 48-49 36.69399358044251 37.0 37.0 40.0 33.0 40.0 50-51 36.54688287680057 37.0 37.0 40.0 33.0 40.0 52-53 36.36591717485766 37.0 37.0 40.0 33.0 40.0 54-55 36.15542540836691 37.0 37.0 40.0 33.0 40.0 56-57 35.9215821098869 37.0 37.0 40.0 33.0 40.0 58-59 35.61725346383429 37.0 33.0 40.0 27.0 40.0 60-61 35.358989506283535 37.0 33.0 40.0 27.0 40.0 62-63 35.047752691358404 37.0 33.0 37.0 27.0 40.0 64-65 34.768783087557175 37.0 33.0 37.0 27.0 40.0 66-67 34.43067824117806 37.0 33.0 37.0 27.0 40.0 68-69 34.136700420330456 37.0 33.0 37.0 27.0 40.0 70-71 33.78813382421435 37.0 33.0 37.0 27.0 37.0 72-73 33.35150321920894 37.0 33.0 37.0 27.0 37.0 74-75 32.91893430614468 37.0 33.0 37.0 24.5 37.0 76-77 30.032149573579886 33.0 30.0 33.0 22.0 37.0 78-79 32.612836142089535 33.0 33.0 37.0 27.0 37.0 80-81 33.062367398033345 37.0 33.0 37.0 27.0 37.0 82-83 32.974765787805524 37.0 33.0 37.0 27.0 37.0 84-85 32.86081097021245 37.0 33.0 37.0 27.0 37.0 86-87 32.77397480318887 37.0 33.0 37.0 27.0 37.0 88-89 32.50973674443851 35.0 33.0 37.0 22.0 37.0 90-91 32.51235769613264 33.0 33.0 37.0 22.0 37.0 92-93 32.37725257515936 33.0 33.0 37.0 22.0 37.0 94-95 32.19863083324782 33.0 33.0 37.0 22.0 37.0 96-97 32.07418054102402 33.0 33.0 37.0 22.0 37.0 98-99 31.947117608454302 33.0 33.0 37.0 22.0 37.0 100-101 30.192674313746362 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.16420492219393168 2306 2 0.12763087824517783 2306 3 0.08229060286530654 2306 4 0.1287533797146878 2306 5 0.14384813967551935 2306 6 0.12753922708157006 2306 7 0.2146500451233635 2306 8 0.1964540792338454 2306 9 0.13244342849564106 2306 10-11 0.17295320796254288 2306 12-13 0.21993539284718366 2306 14-15 0.3949146505689427 2306 16-17 0.3826879686594893 2306 18-19 0.3750960573521027 2306 20-21 0.3645482654180441 2306 22-23 0.4085693775720216 2306 24-25 0.4322828865242059 2306 26-27 0.413133970767781 2306 28-29 0.3091914582670796 2306 30-31 0.29469781535659934 2306 32-33 0.31227243191888476 2306 34-35 0.33036359844235363 2306 36-37 0.2432675395155499 2306 38-39 0.31316012540538196 2306 40-41 0.45755531097685775 2306 42-43 0.31157809204776754 2306 44-45 0.31732521001183756 2306 46-47 0.4987603352620269 2306 48-49 0.45313053189468633 2306 50-51 0.38567234838632913 2306 52-53 0.33631639619586906 2306 54-55 0.34603151475594274 2306 56-57 0.4866659038549912 2306 58-59 0.46711196290701196 2306 60-61 0.45971552779320746 2306 62-63 0.3246300024048736 2306 64-65 0.2985256862910717 2306 66-67 0.2021932948164178 2306 68-69 0.3355406863476915 2306 70-71 0.28049476348696345 2306 72-73 0.16702163347286358 2306 74-75 0.09797883850520606 2306 76-77 -0.2825774804408816 2306 78-79 0.06752038136791327 2306 80-81 0.16766197412272987 2306 82-83 0.34295327100105055 2306 84-85 0.2612823344687385 2306 86-87 0.18966696642551995 2306 88-89 0.6050379196997255 2306 90-91 0.3012063932510216 2306 92-93 0.32245740164272974 2306 94-95 0.3040000134313132 2306 96-97 0.3730782782444919 2306 98-99 0.272424587816154 2306 100-101 0.025079348747805597 2307 1 0.1695864039789896 2307 2 0.10861559844877178 2307 3 0.11385345501400224 2307 4 0.1836657519582232 2307 5 0.12948397152779023 2307 6 0.12862345355228655 2307 7 0.23426474822057486 2307 8 0.1795905924761314 2307 9 0.19039056862342107 2307 10-11 0.18929440676916442 2307 12-13 0.21240295101856077 2307 14-15 0.34866609414951455 2307 16-17 0.31936617730677597 2307 18-19 0.3727759992956763 2307 20-21 0.37907286842921906 2307 22-23 0.38538728949978207 2307 24-25 0.2962262921681784 2307 26-27 0.3042093608260785 2307 28-29 0.37365128757637933 2307 30-31 0.3135061791608891 2307 32-33 0.3677795626904512 2307 34-35 0.36517429863140904 2307 36-37 0.3569867028685607 2307 38-39 0.4190806216838894 2307 40-41 0.3156649184762088 2307 42-43 0.41766751974012806 2307 44-45 0.4003811559644461 2307 46-47 0.5662947496231325 2307 48-49 0.469429747746382 2307 50-51 0.49524502854485775 2307 52-53 0.2952495693275736 2307 54-55 0.2554471253513739 2307 56-57 0.32686778189250987 2307 58-59 0.46096879036839766 2307 60-61 0.3062554992314688 2307 62-63 0.3689080255274888 2307 64-65 0.2856230914112601 2307 66-67 0.4111234691656378 2307 68-69 0.26820719041371177 2307 70-71 0.23886633129709622 2307 72-73 0.1666519502309356 2307 74-75 0.06344302671146096 2307 76-77 -0.33704103490443416 2307 78-79 0.07918714466201493 2307 80-81 0.23003465081600183 2307 82-83 0.3117351122401715 2307 84-85 0.3217089679222269 2307 86-87 0.233475846686801 2307 88-89 0.3744666982822906 2307 90-91 0.23681570253810946 2307 92-93 0.25896451308882007 2307 94-95 0.27706978043924124 2307 96-97 0.21629943045668654 2307 98-99 0.13695208965039996 2307 100-101 0.01355202883968687 2201 1 0.131972231687854 2201 2 0.13021947076182272 2201 3 0.11173927979599796 2201 4 0.1739791155879047 2201 5 0.11870756417523154 2201 6 0.20127777923738677 2201 7 0.18339445991094294 2201 8 0.19358070225974444 2201 9 0.22122351008147945 2201 10-11 0.1695559257882877 2201 12-13 0.20312672675793664 2201 14-15 0.38220638965923825 2201 16-17 0.44132361542462917 2201 18-19 0.36644892100712667 2201 20-21 0.4751619857439877 2201 22-23 0.437823615459358 2201 24-25 0.4021163563576735 2201 26-27 0.41267929765123057 2201 28-29 0.3360333753967666 2201 30-31 0.38737011486342965 2201 32-33 0.34420167967547144 2201 34-35 0.41329430547377655 2201 36-37 0.37964099603289725 2201 38-39 0.5601414101564401 2201 40-41 0.4550074720980817 2201 42-43 0.4167956379127915 2201 44-45 0.3350383122573106 2201 46-47 0.47672734740170597 2201 48-49 0.49334357030916465 2201 50-51 0.3964082314243811 2201 52-53 0.4985232400573878 2201 54-55 0.5039705198172513 2201 56-57 0.517965188751397 2201 58-59 0.5307112063623691 2201 60-61 0.49398099011622065 2201 62-63 0.4407901455434313 2201 64-65 0.5627924055937612 2201 66-67 0.469405078215253 2201 68-69 0.46852569882911155 2201 70-71 0.17309665249172923 2201 72-73 0.26882716693307174 2201 74-75 0.33710400029223564 2201 76-77 0.057043697309794084 2201 78-79 0.189088483151842 2201 80-81 0.23717442313158443 2201 82-83 0.41194672488659023 2201 84-85 0.3919208212223353 2201 86-87 0.4208053972042407 2201 88-89 0.5385250366400811 2201 90-91 0.35090815518299934 2201 92-93 0.41008901625276195 2201 94-95 0.455437094724509 2201 96-97 0.4546194335266449 2201 98-99 0.4113763256887566 2201 100-101 0.4462411907729553 1101 1 0.1224294748318897 1101 2 0.10913198320996997 1101 3 0.14121290759766936 1101 4 0.15563490827219795 1101 5 0.11848427911613157 1101 6 0.13046292274269433 1101 7 0.145742793534545 1101 8 0.07328230410676184 1101 9 0.13159331479636904 1101 10-11 0.1577762992102194 1101 12-13 0.15211457558502417 1101 14-15 0.2202874332042839 1101 16-17 0.29357656167098156 1101 18-19 0.23578607698066634 1101 20-21 0.2906878890437028 1101 22-23 0.18312659095605 1101 24-25 0.2646551521366902 1101 26-27 0.3079543625155452 1101 28-29 0.24847146961413102 1101 30-31 0.32156287238925785 1101 32-33 0.3515432924187891 1101 34-35 0.3365722194554408 1101 36-37 0.3371170263817973 1101 38-39 0.3943560496180609 1101 40-41 0.3795692751695938 1101 42-43 0.3839262909322798 1101 44-45 0.2917623185271623 1101 46-47 0.47118585880487274 1101 48-49 0.4495207514692652 1101 50-51 0.4089173302905351 1101 52-53 0.3478743736756371 1101 54-55 0.33595208540277355 1101 56-57 0.25064496671674164 1101 58-59 0.4904294652747865 1101 60-61 0.5015275785438078 1101 62-63 0.4677165373908565 1101 64-65 0.3437272108222089 1101 66-67 0.4849153515608222 1101 68-69 0.42482390049124064 1101 70-71 0.2113045219503462 1101 72-73 0.3350311869572806 1101 74-75 0.21209585038757695 1101 76-77 -0.17046747810741536 1101 78-79 0.30027149205198356 1101 80-81 0.4385378017024735 1101 82-83 0.5361814153465119 1101 84-85 0.5755738987603038 1101 86-87 0.4001600140694066 1101 88-89 0.7120838432260967 1101 90-91 0.46222424197836176 1101 92-93 0.5480644459648545 1101 94-95 0.5339635473054614 1101 96-97 0.6094245279985557 1101 98-99 0.571600325260043 1101 100-101 0.5687199675280858 2304 1 0.08914934713835265 2304 2 0.11497994809424483 2304 3 0.11006233563703915 2304 4 0.10645227991359008 2304 5 0.13643852226590525 2304 6 0.12231780436015072 2304 7 0.14117309414641 2304 8 0.09437235465211558 2304 9 0.07421568776790366 2304 10-11 0.1130127317720735 2304 12-13 0.20347830264008593 2304 14-15 0.3299801706344567 2304 16-17 0.30648491495643526 2304 18-19 0.24439142414747295 2304 20-21 0.21856897443875312 2304 22-23 0.2768639546165943 2304 24-25 0.1784457214370363 2304 26-27 0.25709076847457624 2304 28-29 0.2568327359083611 2304 30-31 0.2907398476486307 2304 32-33 0.2283053967018489 2304 34-35 0.2878002721290258 2304 36-37 0.2831954381934523 2304 38-39 0.24091065190589944 2304 40-41 0.3091080487795992 2304 42-43 0.4072449927146664 2304 44-45 0.28840204983867324 2304 46-47 0.300341884343581 2304 48-49 0.2944189956831522 2304 50-51 0.2689539091678981 2304 52-53 0.20028226141172922 2304 54-55 0.2923261210505501 2304 56-57 0.25196513165422374 2304 58-59 0.43372422451926695 2304 60-61 0.26959721142488746 2304 62-63 0.35487701890188816 2304 64-65 0.3102931193085041 2304 66-67 0.3094484720716011 2304 68-69 0.390182373489381 2304 70-71 0.3442554397476343 2304 72-73 0.08255047400171378 2304 74-75 0.22259332311968905 2304 76-77 0.04224467938128029 2304 78-79 0.32036260671013395 2304 80-81 0.36662871683947884 2304 82-83 0.49063505993284195 2304 84-85 0.4795024701888764 2304 86-87 0.44534191085046615 2304 88-89 0.7872840294458356 2304 90-91 0.590079389624016 2304 92-93 0.5496123975477261 2304 94-95 0.44273582716712667 2304 96-97 0.44453492970113473 2304 98-99 0.5433191999607558 2304 100-101 0.6290162564347135 2202 1 0.0738414525320934 2202 2 0.08724822080798589 2202 3 0.05676332308307508 2202 4 0.07951547149797022 2202 5 0.10235774024833688 2202 6 -1.3927399047020117E-4 2202 7 0.07643049999169449 2202 8 0.09870210704630011 2202 9 0.13339186501198697 2202 10-11 0.11167614416724803 2202 12-13 0.045038678266543286 2202 14-15 0.07727610672338159 2202 16-17 0.11162273562829483 2202 18-19 -0.019422027944031584 2202 20-21 0.12049747143671397 2202 22-23 0.17683858618509873 2202 24-25 0.15811766854169207 2202 26-27 0.12496025440719194 2202 28-29 0.1781838995068341 2202 30-31 0.1379361087511981 2202 32-33 0.08879558426696832 2202 34-35 0.11538605340228258 2202 36-37 0.1469446938873915 2202 38-39 0.18749968331561462 2202 40-41 0.3044857220788586 2202 42-43 0.27185150291166593 2202 44-45 0.28800105494957506 2202 46-47 0.3630430950814372 2202 48-49 0.2758605924677511 2202 50-51 0.24980824847221328 2202 52-53 0.238251360305199 2202 54-55 0.11432433471600945 2202 56-57 0.17020373136642064 2202 58-59 0.3290938965284056 2202 60-61 0.25792072405328526 2202 62-63 0.1666988940569567 2202 64-65 0.08717255413210268 2202 66-67 0.13681710205224817 2202 68-69 0.21467612131848313 2202 70-71 0.18569157828780192 2202 72-73 0.2474403167050312 2202 74-75 0.2730612435285664 2202 76-77 -0.0674161651579972 2202 78-79 0.3257981498054576 2202 80-81 0.36476516054512587 2202 82-83 0.3414692409480722 2202 84-85 0.47414979316808825 2202 86-87 0.5702924765230648 2202 88-89 0.606847245840413 2202 90-91 0.38416699444711355 2202 92-93 0.46346486368179995 2202 94-95 0.34395524859321824 2202 96-97 0.4277007114705995 2202 98-99 0.3732681529702404 2202 100-101 0.5077550918438298 1102 1 0.10854794323017813 1102 2 0.040820870120427344 1102 3 0.05538675225627543 1102 4 0.04408223627860508 1102 5 0.08572518513822303 1102 6 0.04227748942939513 1102 7 0.08220847642681406 1102 8 0.11103000974603106 1102 9 0.14878265798228085 1102 10-11 0.12414842708605534 1102 12-13 0.09750007270269379 1102 14-15 0.14041177754016587 1102 16-17 0.145457121368878 1102 18-19 0.14044008664035346 1102 20-21 0.1175122489597129 1102 22-23 0.1037367017234132 1102 24-25 0.04299727711907053 1102 26-27 0.04434010446394865 1102 28-29 0.07803459513810651 1102 30-31 0.05548064635855354 1102 32-33 0.13860918156240842 1102 34-35 0.07635628616809953 1102 36-37 0.09435010523954901 1102 38-39 0.21729297973742945 1102 40-41 0.22704084141856384 1102 42-43 0.14314045847068968 1102 44-45 0.2236242562511208 1102 46-47 0.3508678756165793 1102 48-49 0.1956848845088004 1102 50-51 0.14942772817757088 1102 52-53 0.1371943748626876 1102 54-55 0.18245323165574945 1102 56-57 0.09015285694353992 1102 58-59 0.1962588479303946 1102 60-61 0.13400363335620824 1102 62-63 0.18477623157500744 1102 64-65 0.16028055053597967 1102 66-67 0.2495395363857753 1102 68-69 0.24495992993028182 1102 70-71 0.15342115782769383 1102 72-73 0.21111715964010358 1102 74-75 0.1265365976406585 1102 76-77 0.18138525991628285 1102 78-79 0.29355033199945524 1102 80-81 0.16500307654352042 1102 82-83 0.3567000855446736 1102 84-85 0.22066022014144693 1102 86-87 0.2182789721291556 1102 88-89 0.5119287837619027 1102 90-91 0.2830660145528725 1102 92-93 0.33214061799925076 1102 94-95 0.4284216552832376 1102 96-97 0.2493736699781266 1102 98-99 0.24619412881676084 1102 100-101 0.24738871896554215 2305 1 0.10497401621726965 2305 2 0.09160810272256015 2305 3 0.03491302038031563 2305 4 0.04663193914502983 2305 5 0.04636249057076469 2305 6 0.006471264718321379 2305 7 -0.013865494228980424 2305 8 0.06333939995052162 2305 9 0.05909992264171393 2305 10-11 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-1.0288130881794793 1213 88-89 -0.8119248886166339 1213 90-91 -0.8479292438882382 1213 92-93 -0.8912347052137051 1213 94-95 -0.8153463690431835 1213 96-97 -0.8455743634502575 1213 98-99 -0.89556725480222 1213 100-101 -0.8838766977139016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 15.0 10 26.0 11 24.0 12 42.0 13 93.0 14 276.0 15 769.0 16 1381.0 17 2379.0 18 3309.0 19 4564.0 20 6122.0 21 7920.0 22 9945.0 23 12991.0 24 16717.0 25 20919.0 26 26960.0 27 35772.0 28 50596.0 29 71756.0 30 72578.0 31 75954.0 32 99113.0 33 140515.0 34 216319.0 35 383430.0 36 727872.0 37 946493.0 38 193378.0 39 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.874723799052184 25.578072854613843 18.306507568090076 16.2406957782439 2 32.96958398185825 23.68373018422743 28.12636957432791 15.220316259586422 3 38.731997482292776 18.88849427617995 24.117787524818244 18.26172071670903 4 33.28681229191426 18.630030467285476 27.44085992626335 20.642297314536915 5 36.81751444016837 17.045616195365273 26.933691104907435 19.20317825955893 6 32.92067489426207 17.865658564061153 29.717896858086608 19.49576968359017 7 34.858401798064286 20.251878604447914 23.293352551727754 21.596367045760047 8 31.67397266141837 25.778541569367952 21.76352104513286 20.78396472408082 9 31.813603311496863 22.17461311473209 24.4113887207972 21.60039485297385 10-11 24.866310545055946 28.657917227219087 28.626238056976796 17.84953417074817 12-13 23.780173790291258 27.470955833815232 28.213047346234465 20.535823029659046 14-15 24.036562899484153 27.579658678502028 27.85270245085676 20.531075971157065 16-17 24.964280822186026 26.143752927981602 28.22388056600212 20.668085683830252 18-19 23.169673296884874 26.054094729550325 29.32389100242212 21.452340971142682 20-21 23.895637597086935 26.428217282937155 29.051870165232803 20.62427495474311 22-23 22.573161311235083 28.94730154305542 27.66026855663158 20.81926858907792 24-25 22.659036849001662 25.680371760212488 29.531754624705865 22.12883676607998 26-27 22.35043009628697 25.77537686369996 28.66211588384955 23.21207715616352 28-29 23.38619990135069 27.928751287060223 27.75544369333299 20.9296051182561 30-31 21.543046550263035 27.75221505421716 28.87909002245023 21.82564837306957 32-33 21.279081327501324 28.89520125130544 27.80854042176256 22.017176999430674 34-35 21.63648528427848 28.727919385036955 29.23368090752251 20.401914423162065 36-37 21.11941400653278 29.121217584663 29.673191770671302 20.086176638132923 38-39 21.293649105236398 27.67817312578029 29.10175839137071 21.926419377612604 40-41 22.71964469331286 27.283592080788605 27.671365610178274 22.32539761572027 42-43 21.92515967420532 28.257047698707854 28.58757678020016 21.230215846886665 44-45 21.4339704308212 27.064850065860064 28.788448139974655 22.712731363344083 46-47 22.58417864423544 27.184965246109588 28.095410721838128 22.135445387816848 48-49 21.25208016410179 28.73852902687082 28.929909718760054 21.079481090267333 50-51 22.67507683130392 27.553790703967675 27.713439336316913 22.057693128411497 52-53 21.533995725030564 26.937965631777537 27.22336851160384 24.304670131588054 54-55 23.047309337761344 26.86018645982725 28.82411445648706 21.26838974592435 56-57 22.114021185420548 27.081590182231963 28.015306372027904 22.78908226031959 58-59 21.98616572355619 27.602559667271393 27.976793023646927 22.434481585525486 60-61 22.966129045406962 27.35333783788752 28.144735897732232 21.535797218973286 62-63 21.73320959993044 27.132842811160685 28.527305048763985 22.606642540144893 64-65 23.16908501687498 27.303467183826108 28.511003785181256 21.01644401411766 66-67 22.31574780262141 29.545073001862022 27.19330951900536 20.945869676511208 68-69 22.577788264753945 29.20315926551521 27.127569269195245 21.091483200535603 70-71 22.07137994251052 29.51736723905652 27.142112976511434 21.269139841921525 72-73 21.977478919261838 29.72415257367107 26.87133419305163 21.427034314015465 74-75 22.395953574244693 28.95564753108171 26.833886401556306 21.81451249311729 76-77 22.111578150609926 28.36247253684632 26.804149920158792 22.72179939238497 78-79 21.796857315686694 28.38910572765534 27.187867274213083 22.626169682444885 80-81 21.28243746689076 28.468449948735625 27.72183152738432 22.5272810569893 82-83 20.80314388497143 28.501934719345417 27.692164086960958 23.002757308722195 84-85 20.808094597026884 29.117368076284787 27.42092857236483 22.6536087543235 86-87 20.6367626290546 29.17835409280261 27.6515363456705 22.53334693247228 88-89 20.951190038656815 29.077929263515855 27.605306283422653 22.36557441440468 90-91 21.454310532537267 28.903958610241858 26.820641930055505 22.82108892716537 92-93 21.442152422772388 28.85889428115995 26.7984252671045 22.900528028963162 94-95 21.738191342089483 29.122872201725343 26.65441596730617 22.484520488879006 96-97 21.764838579867995 29.844022864261472 26.468373945434465 21.92276461043607 98-99 21.910168859290426 29.44653657861156 26.695536039629193 21.947758522468817 100-101 21.99497567366017 29.514408370786647 26.363901582031357 22.126714373521818 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 31.0 1 19.0 2 24.5 3 60.5 4 55.5 5 31.0 6 30.0 7 29.5 8 34.0 9 39.0 10 38.5 11 32.0 12 32.5 13 35.0 14 30.5 15 41.5 16 61.0 17 82.5 18 112.5 19 169.0 20 246.5 21 344.0 22 459.0 23 662.0 24 963.0 25 1352.5 26 1944.0 27 2715.0 28 3591.0 29 4693.0 30 6289.0 31 8576.0 32 13159.5 33 21757.0 34 36034.0 35 56104.5 36 85986.0 37 121956.5 38 145338.5 39 150146.5 40 153460.0 41 157614.5 42 165419.5 43 179746.5 44 178485.0 45 168358.0 46 164778.5 47 160293.0 48 150991.5 49 135226.0 50 120698.0 51 116874.5 52 112932.0 53 102317.5 54 88356.0 55 73062.0 56 59286.0 57 45189.0 58 32092.5 59 23239.5 60 17636.0 61 14234.0 62 11312.5 63 8632.5 64 6516.0 65 4731.0 66 3427.0 67 2542.0 68 1978.0 69 1508.0 70 1044.0 71 785.0 72 563.0 73 393.0 74 296.5 75 223.0 76 171.0 77 132.5 78 101.0 79 80.0 80 62.0 81 43.5 82 33.5 83 21.0 84 12.5 85 14.5 86 15.0 87 10.0 88 8.5 89 7.0 90 4.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16219915716535716 2 0.0 3 3.1966723919069203E-4 4 0.020330836412528013 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.635174968265035E-4 12-13 0.0 14-15 0.0 16-17 0.00100695180345068 18-19 0.0 20-21 0.0 22-23 3.6761732506929585E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.631015458148685E-4 38-39 7.831847360171956E-4 40-41 0.004427391262791085 42-43 0.0027651216189994864 44-45 9.430183556125416E-4 46-47 0.0036442065267738894 48-49 0.00621752780225896 50-51 0.004267557643195739 52-53 0.0013905524904795103 54-55 0.00917444976477286 56-57 0.017134164020621095 58-59 0.021066071062666607 60-61 0.009829767605113782 62-63 7.032679262195225E-4 64-65 0.012706772757830009 66-67 0.03209459081474548 68-69 0.01802923229035503 70-71 0.03426832804124219 72-73 0.027874983257428345 74-75 0.027203682055127895 76-77 0.035435113464288216 78-79 0.01537599420507229 80-81 0.01118835337167422 82-83 6.872845642599879E-4 84-85 0.00927034993653007 86-87 0.0193079012471178 88-89 0.019259951161239196 90-91 0.02288817432605355 92-93 0.03984652136511977 94-95 0.02597296318424373 96-97 0.0268360647300586 98-99 0.0339966108879301 100-101 0.015040343603922061 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3128253.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.325340354287 #Duplication Level Percentage of deduplicated Percentage of total 1 85.38729873849643 40.40982974732525 2 7.203847130863411 6.818490346567296 3 2.288257893653796 3.248777509066493 4 1.0733831849705124 2.0319289823719244 5 0.6811723455115848 1.6118356545631867 6 0.5402245060953659 1.5339785171213869 7 0.3668099453379062 1.2151583855917671 8 0.26986437128194357 1.0217138576330922 9 0.2051297159228476 0.8737050260544271 >10 1.612592126700429 14.864259406170612 >50 0.20362031098012023 6.660564583384923 >100 0.15935174537856595 14.250135337762107 >500 0.00677050184100983 2.179897996527183 >1k 0.0016096088689246332 1.1405835393339554 >5k 0.0 0.0 >10k+ 6.787409702373702E-5 2.139141110526432 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 66595 2.128823979390414 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3952 0.1263324929281615 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3728 0.11917194677029 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011188353371674221 0.0 0.0 2 0.0013106356806818374 0.0 0.0 3 0.0014704693002771834 0.0 0.0 4 0.0015983361959534604 0.0 0.0 5 0.0018860367112250832 0.0 0.0 6 0.002173737226496706 0.0 0.0 7 0.0026852048092018133 0.0 0.0 8 0.002940938600554367 0.0 0.0 9 0.0033245392875831973 0.0 0.0 10-11 0.004858942035698519 0.0 0.0 12-13 0.00666506193712593 0.0 0.0 14-15 0.008758882353824961 0.0 0.0 16-17 0.010820736046604926 0.0 0.0 18-19 0.012802672929587217 0.0 0.0 20-21 0.015360010843112754 0.0 0.0 22-23 0.01833291616758619 0.0 0.0 24-25 0.022168923037874495 0.0 0.0 26-27 0.026836064730058597 0.0 0.0 28-29 0.03249417486373385 0.0 0.0 30-31 0.06575555110152537 0.0 0.0 32-33 0.0733955981181829 0.0 0.0 34-35 0.07999672660747069 0.0 0.0 36-37 0.08699743914574684 0.0 0.0 38-39 0.09577230486153135 1.5983361959534603E-5 0.0 40-41 0.1061455067732693 3.1966723919069206E-5 0.0 42-43 0.11877236272130162 3.1966723919069206E-5 0.0 44-45 0.1328856713315707 3.1966723919069206E-5 0.0 46-47 0.14861329949975274 3.1966723919069206E-5 0.0 48-49 0.16478846180280177 3.1966723919069206E-5 0.0 50-51 0.18371276236289072 3.1966723919069206E-5 0.0 52-53 0.2037399148981876 3.1966723919069206E-5 0.0 54-55 0.22790675818100392 6.393344783813841E-5 0.0 56-57 0.2528088361139588 6.393344783813841E-5 0.0 58-59 0.28146700410740433 6.393344783813841E-5 0.0 60-61 0.31213907570775123 6.393344783813841E-5 0.0 62-63 0.3457840526325716 6.393344783813841E-5 0.0 64-65 0.3825457851395012 6.393344783813841E-5 0.0 66-67 0.4216730552164419 6.393344783813841E-5 0.0 68-69 0.4664744187890174 6.393344783813841E-5 0.0 70-71 0.5164543916364821 6.393344783813841E-5 0.0 72-73 0.5720605078937029 6.393344783813841E-5 0.0 74-75 0.6337882517814255 6.393344783813841E-5 0.0 76-77 0.7034117764771584 6.393344783813841E-5 0.0 78-79 0.7829130188638835 6.393344783813841E-5 0.0 80-81 0.8730591803156587 6.393344783813841E-5 0.0 82-83 0.9749531128076917 6.393344783813841E-5 0.0 84-85 1.090816503652358 6.393344783813841E-5 0.0 86-87 1.2225513729228423 6.393344783813841E-5 0.0 88-89 1.3658102461661508 6.393344783813841E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCG 25 0.0049914187 57.088264 1 CGGAAGA 13805 0.0 51.8254 4 TCGGAAG 13840 0.0 51.660015 3 GAAGAGC 13905 0.0 51.265472 6 ATCGGAA 14305 0.0 49.98075 2 GATCGGA 14395 0.0 49.93654 1 GGAAGAG 15985 0.0 44.802677 5 GCCGTCT 8280 0.0 42.796043 50-51 ATGCCGT 8250 0.0 42.629543 48-49 TATGCCG 8305 0.0 42.261444 48-49 TGCCGTC 8380 0.0 42.001934 50-51 GTATGCC 8555 0.0 41.386982 46-47 GATCTCG 8355 0.0 41.382248 40-41 TCGTATG 8620 0.0 41.15754 44-45 TCGATCT 8410 0.0 40.998676 38-39 TCTCGTA 8485 0.0 40.77686 42-43 CGTATGC 8705 0.0 40.510143 46-47 ACTCGAT 8695 0.0 40.364914 36-37 CTCGATC 8495 0.0 40.253 38-39 TACTCGA 8780 0.0 39.86595 36-37 >>END_MODULE