FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_30521-1.341000000356df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_30521-1.341000000356df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128253
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT665952.128823979390414TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC39520.1263324929281615No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA37280.11917194677029No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG250.004991418757.0882641
CGGAAGA138050.051.82544
TCGGAAG138400.051.6600153
GAAGAGC139050.051.2654726
ATCGGAA143050.049.980752
GATCGGA143950.049.936541
GGAAGAG159850.044.8026775
GCCGTCT82800.042.79604350-51
ATGCCGT82500.042.62954348-49
TATGCCG83050.042.26144448-49
TGCCGTC83800.042.00193450-51
GTATGCC85550.041.38698246-47
GATCTCG83550.041.38224840-41
TCGTATG86200.041.1575444-45
TCGATCT84100.040.99867638-39
TCTCGTA84850.040.7768642-43
CGTATGC87050.040.51014346-47
ACTCGAT86950.040.36491436-37
CTCGATC84950.040.25338-39
TACTCGA87800.039.8659536-37
Produced by FastQC (version 0.11.2)