FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_30484-1.34100000035806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_30484-1.34100000035806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8572676
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3144103.667582911100338TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG476300.071.16363
CGGAAGA475650.071.131034
GAAGAGC488000.069.348766
GATCGGA490750.069.13881
ATCGGAA506850.066.902382
GGAAGAG553150.061.2924775
ACGTTCG405.3488644E-447.574121
AGAGCAC764000.044.4825558
GAGCACA764800.044.4236039
AAGAGCA768500.044.141757
GCCGTCT386950.043.57313550-51
CGTCTTC387850.043.40643352-53
ATGCCGT388050.043.35988248-49
TGCCGTC387850.043.2209550-51
CCGTCTT387650.043.18987352-53
TATGCCG389050.043.06530448-49
TCTCGTA386500.042.93700442-43
TCGTATG393900.042.84203344-45
CGTATGC391400.042.8001746-47
CTCGTAT388550.042.6373744-45
Produced by FastQC (version 0.11.2)