##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_30484-1.34100000035806.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8572676 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67450747001286 33.0 33.0 33.0 33.0 33.0 2 32.71253036974686 33.0 33.0 33.0 33.0 33.0 3 32.739409491272035 33.0 33.0 33.0 33.0 33.0 4 36.65330440576548 37.0 37.0 37.0 37.0 37.0 5 36.656997418309054 37.0 37.0 37.0 37.0 37.0 6 36.54769257580713 37.0 37.0 37.0 37.0 37.0 7 36.470795233600334 37.0 37.0 37.0 37.0 37.0 8 36.42788132900392 37.0 37.0 37.0 37.0 37.0 9 36.44951004797102 37.0 37.0 37.0 37.0 37.0 10-11 36.3930185277036 37.0 37.0 37.0 37.0 37.0 12-13 36.36584422413725 37.0 37.0 37.0 37.0 37.0 14-15 38.23083818868227 40.0 37.0 40.0 37.0 40.0 16-17 38.22796055747354 40.0 37.0 40.0 37.0 40.0 18-19 38.207000649505474 40.0 37.0 40.0 37.0 40.0 20-21 38.159699258434586 40.0 37.0 40.0 37.0 40.0 22-23 37.992081877350785 40.0 37.0 40.0 33.0 40.0 24-25 38.015594255516014 40.0 37.0 40.0 35.0 40.0 26-27 37.92980715706507 40.0 37.0 40.0 33.0 40.0 28-29 37.77702610013489 40.0 37.0 40.0 33.0 40.0 30-31 37.72089391923829 40.0 37.0 40.0 33.0 40.0 32-33 37.581018984037186 40.0 37.0 40.0 33.0 40.0 34-35 37.46459950195248 40.0 37.0 40.0 33.0 40.0 36-37 37.30122758634526 40.0 37.0 40.0 33.0 40.0 38-39 37.265060116584365 40.0 37.0 40.0 33.0 40.0 40-41 37.23081089265476 40.0 37.0 40.0 33.0 40.0 42-43 36.9804114257905 38.5 37.0 40.0 33.0 40.0 44-45 36.898870900988214 37.0 37.0 40.0 33.0 40.0 46-47 36.792560747659195 37.0 37.0 40.0 33.0 40.0 48-49 36.62739983407748 37.0 37.0 40.0 33.0 40.0 50-51 36.526455624824735 37.0 37.0 40.0 33.0 40.0 52-53 36.38368241141973 37.0 37.0 40.0 33.0 40.0 54-55 36.167274489319325 37.0 37.0 40.0 33.0 40.0 56-57 35.96240613782675 37.0 37.0 40.0 30.0 40.0 58-59 35.71756753667116 37.0 37.0 40.0 27.0 40.0 60-61 35.40479139769192 37.0 33.0 40.0 27.0 40.0 62-63 35.14413626503556 37.0 33.0 38.5 27.0 40.0 64-65 34.874445039098646 37.0 33.0 37.0 27.0 40.0 66-67 34.448165893590286 37.0 33.0 37.0 27.0 40.0 68-69 34.16368867784108 37.0 33.0 37.0 27.0 40.0 70-71 33.795409216445364 37.0 33.0 37.0 27.0 40.0 72-73 33.235857974802734 37.0 33.0 37.0 24.5 37.0 74-75 32.6222607153239 37.0 33.0 37.0 22.0 37.0 76-77 29.93698542905389 33.0 30.0 35.0 22.0 37.0 78-79 32.26900322606383 35.0 33.0 37.0 22.0 37.0 80-81 32.602514722357405 37.0 33.0 37.0 22.0 37.0 82-83 32.47450720171858 37.0 33.0 37.0 22.0 37.0 84-85 32.319809882001834 37.0 33.0 37.0 22.0 37.0 86-87 32.209284242166625 35.0 33.0 37.0 22.0 37.0 88-89 31.937784187807868 33.0 33.0 37.0 22.0 37.0 90-91 31.904441623595716 33.0 33.0 37.0 22.0 37.0 92-93 31.75329996141228 33.0 33.0 37.0 18.5 37.0 94-95 31.564572369234533 33.0 33.0 37.0 15.0 37.0 96-97 31.45241969951973 33.0 33.0 37.0 15.0 37.0 98-99 31.326527970962623 33.0 33.0 37.0 15.0 37.0 100-101 29.675323259621617 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.11328661210286839 2306 2 0.10522323096816422 2306 3 0.11537827657356559 2306 4 0.12469217139172173 2306 5 0.1655372499438954 2306 6 0.1447096181710421 2306 7 0.17430677146770535 2306 8 0.18478032723635351 2306 9 0.18048567118324144 2306 10-11 0.14152732108558297 2306 12-13 0.2012098773174884 2306 14-15 0.3557226584566848 2306 16-17 0.40845423534259595 2306 18-19 0.34264898636298824 2306 20-21 0.33718148308891216 2306 22-23 0.4195183302490122 2306 24-25 0.3820027100724772 2306 26-27 0.3768740595043738 2306 28-29 0.35436859067836224 2306 30-31 0.3991611497550025 2306 32-33 0.3448770106141694 2306 34-35 0.41744788619955386 2306 36-37 0.3835696906726014 2306 38-39 0.49300079888286774 2306 40-41 0.4552342725660026 2306 42-43 0.4166415730230213 2306 44-45 0.4001692339055509 2306 46-47 0.5564906952939168 2306 48-49 0.42281240255167774 2306 50-51 0.43027109625568016 2306 52-53 0.42092927076343045 2306 54-55 0.43211768959327657 2306 56-57 0.43802131043504033 2306 58-59 0.4727966297830122 2306 60-61 0.431147284233262 2306 62-63 0.431765876134925 2306 64-65 0.29449021304459677 2306 66-67 0.2609312343185479 2306 68-69 0.36242665340737545 2306 70-71 0.3159784051906982 2306 72-73 0.29344740264076563 2306 74-75 0.3705969407227059 2306 76-77 -0.05337100593380839 2306 78-79 0.2295337791078822 2306 80-81 0.46008271160203407 2306 82-83 0.48952864242863114 2306 84-85 0.520883183300306 2306 86-87 0.4838887606504727 2306 88-89 0.7455683385247873 2306 90-91 0.49888012818121297 2306 92-93 0.5150106924611606 2306 94-95 0.35126912798384424 2306 96-97 0.4775362634893838 2306 98-99 0.4578016414462027 2306 100-101 0.21721598195121672 2307 1 0.1405965085539833 2307 2 0.11520207705346053 2307 3 0.10196653895749819 2307 4 0.1550492476691403 2307 5 0.13420405345750197 2307 6 0.1786713411805465 2307 7 0.18927594198267883 2307 8 0.2128733651277983 2307 9 0.17923913486532683 2307 10-11 0.19904687606261007 2307 12-13 0.2061308359180103 2307 14-15 0.3478479774759933 2307 16-17 0.365402925524819 2307 18-19 0.3485346339460662 2307 20-21 0.3859681137164799 2307 22-23 0.41305182066952284 2307 24-25 0.3094342118283322 2307 26-27 0.44297674469536474 2307 28-29 0.45868324358495016 2307 30-31 0.4229768603573163 2307 32-33 0.40766250959129735 2307 34-35 0.4108514949435005 2307 36-37 0.3795816676479049 2307 38-39 0.45489040109668366 2307 40-41 0.4217737617171906 2307 42-43 0.48060375082324924 2307 44-45 0.42725218568585177 2307 46-47 0.49444171146439686 2307 48-49 0.4302980530847407 2307 50-51 0.44333752069507426 2307 52-53 0.37032798415636137 2307 54-55 0.34666943596919 2307 56-57 0.262535759873991 2307 58-59 0.40722088725519967 2307 60-61 0.4136042849990247 2307 62-63 0.4939425291455777 2307 64-65 0.38359191121679004 2307 66-67 0.3318893226897188 2307 68-69 0.2912146197266381 2307 70-71 0.19308813226300714 2307 72-73 0.09969352309147439 2307 74-75 0.1118799340487584 2307 76-77 -0.20301501951525225 2307 78-79 0.15195258564687464 2307 80-81 0.2003286591503226 2307 82-83 0.3744590082319732 2307 84-85 0.27801354394116373 2307 86-87 0.3333925266045128 2307 88-89 0.4814805401503186 2307 90-91 0.29922253233611684 2307 92-93 0.27098617145617965 2307 94-95 0.3903712153913439 2307 96-97 0.29722636662854995 2307 98-99 0.24431107343716718 2307 100-101 0.3030650659985916 2201 1 0.12647519176687894 2201 2 0.12749692468111817 2201 3 0.11053282962285493 2201 4 0.16689681412018587 2201 5 0.14834102082448908 2201 6 0.12607377104928474 2201 7 0.1594968398455947 2201 8 0.18958855633992044 2201 9 0.15188850686644173 2201 10-11 0.17479393944823585 2201 12-13 0.15073297415911924 2201 14-15 0.3041878181964748 2201 16-17 0.3127424044224867 2201 18-19 0.25636178336339555 2201 20-21 0.3177347620208337 2201 22-23 0.35209265706130566 2201 24-25 0.3549914615720766 2201 26-27 0.33138276412532974 2201 28-29 0.28188425978795095 2201 30-31 0.22527877398936624 2201 32-33 0.33237260584236594 2201 34-35 0.35954265709202815 2201 36-37 0.4315450793813369 2201 38-39 0.44413554728626536 2201 40-41 0.35358461128799945 2201 42-43 0.3924117646949341 2201 44-45 0.345706853626254 2201 46-47 0.3860858126291191 2201 48-49 0.30933875001591105 2201 50-51 0.38220503853634824 2201 52-53 0.3043443645801176 2201 54-55 0.40568331772363564 2201 56-57 0.37017990597602335 2201 58-59 0.4606984538534604 2201 60-61 0.3690037737984042 2201 62-63 0.4978903104434451 2201 64-65 0.46664314672160145 2201 66-67 0.3905837621626489 2201 68-69 0.3218605857896719 2201 70-71 0.1895430434846972 2201 72-73 0.27210554087734806 2201 74-75 0.27217394801186856 2201 76-77 -0.023225341615031425 2201 78-79 0.3082018157080668 2201 80-81 0.45392592944126164 2201 82-83 0.4787222029492071 2201 84-85 0.5114444997034795 2201 86-87 0.4896789074234107 2201 88-89 0.7243961347135262 2201 90-91 0.38367248030955636 2201 92-93 0.6134532839319462 2201 94-95 0.5853907669944824 2201 96-97 0.4758293896697694 2201 98-99 0.5409805603073359 2201 100-101 0.43853210796926234 1101 1 0.12595659421346994 1101 2 0.07832155268869201 1101 3 0.10788594189743606 1101 4 0.13813546549084776 1101 5 0.11176472963789053 1101 6 0.09933746177656388 1101 7 0.1150169675512771 1101 8 0.08722426430415453 1101 9 0.07788689676026905 1101 10-11 0.1068731682514894 1101 12-13 0.14260896841346238 1101 14-15 0.24328176127480816 1101 16-17 0.267190736280817 1101 18-19 0.243502232042637 1101 20-21 0.21487385975483164 1101 22-23 0.2347269451545131 1101 24-25 0.2428287498665469 1101 26-27 0.21824089281803083 1101 28-29 0.28577870409454675 1101 30-31 0.3633983133446037 1101 32-33 0.38029332189848475 1101 34-35 0.3199001337468701 1101 36-37 0.2911046712304497 1101 38-39 0.377189455515726 1101 40-41 0.3923414383863104 1101 42-43 0.30549153387628536 1101 44-45 0.2880580221938942 1101 46-47 0.30945578690602105 1101 48-49 0.33430973226339233 1101 50-51 0.23411917631622003 1101 52-53 0.18064575219388246 1101 54-55 0.23381751069926793 1101 56-57 0.18954770495537332 1101 58-59 0.29571605749715246 1101 60-61 0.13159506599131987 1101 62-63 0.31874718192995033 1101 64-65 0.23795106849911463 1101 66-67 0.30021159053194424 1101 68-69 0.30958676573006727 1101 70-71 0.22066737456002272 1101 72-73 0.20607113037196712 1101 74-75 0.08669488628469679 1101 76-77 -0.1992063622128093 1101 78-79 0.26619272577647024 1101 80-81 0.3592893888536324 1101 82-83 0.4038579867318006 1101 84-85 0.4486352642006395 1101 86-87 0.3333721987395961 1101 88-89 0.6610178479878037 1101 90-91 0.428390422240966 1101 92-93 0.4305740336238415 1101 94-95 0.566403714012619 1101 96-97 0.5570409807799948 1101 98-99 0.35571144649984987 1101 100-101 0.29621354129460187 2304 1 0.12630577954449507 2304 2 0.11001828849551032 2304 3 0.0986914541375512 2304 4 0.11440925389276657 2304 5 0.09316087083750091 2304 6 0.13161351129264176 2304 7 0.1314163368398127 2304 8 0.12175295848169299 2304 9 0.08113531248004335 2304 10-11 0.09860161386134081 2304 12-13 0.13152808131333416 2304 14-15 0.2680931576386385 2304 16-17 0.2705053350830795 2304 18-19 0.23811369500091928 2304 20-21 0.2803963213492864 2304 22-23 0.2847941445768498 2304 24-25 0.2623750218836136 2304 26-27 0.21341448850255063 2304 28-29 0.23732542814084923 2304 30-31 0.24790568858404782 2304 32-33 0.15212877841414496 2304 34-35 0.28937944832728135 2304 36-37 0.31574413677120816 2304 38-39 0.3107867710234302 2304 40-41 0.3133187168992464 2304 42-43 0.3428849754836918 2304 44-45 0.24248401281066378 2304 46-47 0.3136097130268283 2304 48-49 0.35651663679229273 2304 50-51 0.2755078014407246 2304 52-53 0.2578504352942872 2304 54-55 0.2881736642498254 2304 56-57 0.20638700420357026 2304 58-59 0.3410815962201852 2304 60-61 0.24135468709982888 2304 62-63 0.23819438501110568 2304 64-65 0.24197783114698268 2304 66-67 0.2862983820825775 2304 68-69 0.240262709159623 2304 70-71 0.2271857427247781 2304 72-73 0.022589726003246824 2304 74-75 0.040733457833567854 2304 76-77 0.03018504865079663 2304 78-79 0.19817744873675025 2304 80-81 0.23468515642735355 2304 82-83 0.3679389638505981 2304 84-85 0.43484244362527136 2304 86-87 0.4046916691974971 2304 88-89 0.661844713044001 2304 90-91 0.48222715353716694 2304 92-93 0.3958028697985725 2304 94-95 0.47443088706819125 2304 96-97 0.4045979901854935 2304 98-99 0.3455517988697103 2304 100-101 0.49075562695946573 2202 1 0.054942405934511385 2202 2 0.04630071348120168 2202 3 0.011949584286703896 2202 4 0.051394045753802686 2202 5 0.04609379794889179 2202 6 0.03394864322399371 2202 7 0.07848500568400851 2202 8 0.09774568678093942 2202 9 0.06457390567301502 2202 10-11 0.0816250023708065 2202 12-13 0.09079640302987002 2202 14-15 0.07079622322040535 2202 16-17 0.07080436444924487 2202 18-19 0.043445548341679796 2202 20-21 0.12746735484326166 2202 22-23 0.1390917820940487 2202 24-25 0.12229122835868367 2202 26-27 0.07692620720908394 2202 28-29 0.10742902908518914 2202 30-31 0.11402727053036443 2202 32-33 0.12347830088666711 2202 34-35 0.11921288034631061 2202 36-37 0.07520903189789152 2202 38-39 0.1245615954536774 2202 40-41 0.18398284233607853 2202 42-43 0.26389677764845487 2202 44-45 0.2742920298958751 2202 46-47 0.2264409250569912 2202 48-49 0.19545462937886526 2202 50-51 0.22242511073574178 2202 52-53 0.11402643200514717 2202 54-55 0.15887596816545368 2202 56-57 0.18092536306926377 2202 58-59 0.24902702449559655 2202 60-61 0.20178007661548492 2202 62-63 0.19705621391178596 2202 64-65 0.14746274248505387 2202 66-67 0.07277680212754944 2202 68-69 0.08416957768393019 2202 70-71 -0.10634928132584065 2202 72-73 -0.041236125080331476 2202 74-75 0.02016521418708095 2202 76-77 -0.30120861297499246 2202 78-79 0.04791951156645524 2202 80-81 0.13931685380372016 2202 82-83 0.20355769826075232 2202 84-85 0.23363234324698823 2202 86-87 0.26859417739544256 2202 88-89 0.486432356189507 2202 90-91 0.23903987063837917 2202 92-93 0.3167690261694247 2202 94-95 0.20980391752722127 2202 96-97 0.12444164071280639 2202 98-99 0.1812429003484759 2202 100-101 0.27376807804855474 1102 1 0.08432504306737343 1102 2 0.07319848295767173 1102 3 0.06959643020292816 1102 4 0.13115404806393371 1102 5 0.09224286835079454 1102 6 0.05787641749792982 1102 7 0.09367210005746074 1102 8 0.09143883179483225 1102 9 0.08508703371531823 1102 10-11 0.07318171084079239 1102 12-13 0.06577199734720551 1102 14-15 0.1558761393206467 1102 16-17 0.1369017784805422 1102 18-19 0.10522800486428707 1102 20-21 0.08659416955958932 1102 22-23 0.12697014810938612 1102 24-25 0.0666222763903761 1102 26-27 0.1628857533339385 1102 28-29 0.14971769474161079 1102 30-31 0.18609284609757282 1102 32-33 0.16297721243423524 1102 34-35 0.10139683044747017 1102 36-37 0.1248779021584312 1102 38-39 0.15825129824085593 1102 40-41 0.11539721358334987 1102 42-43 0.09811197785216308 1102 44-45 0.028049167840244138 1102 46-47 0.13909377436096548 1102 48-49 -0.001971641669086921 1102 50-51 0.05772111762151155 1102 52-53 0.03802644709975311 1102 54-55 -0.0062998790849988495 1102 56-57 0.06179578702427335 1102 58-59 0.21406709411454727 1102 60-61 0.13494063959182512 1102 62-63 0.1563880281588652 1102 64-65 0.12126976810585433 1102 66-67 0.1842567430354407 1102 68-69 0.0716227844019457 1102 70-71 -0.18190733879428223 1102 72-73 -0.09294025699932718 1102 74-75 0.0021842475021927044 1102 76-77 -0.13131628346365787 1102 78-79 0.035279705971866804 1102 80-81 0.09312035023975085 1102 82-83 0.1709713210123951 1102 84-85 0.1585414474608129 1102 86-87 0.07305382163523433 1102 88-89 0.27094865278672486 1102 90-91 0.0547219286633549 1102 92-93 0.15539916104301454 1102 94-95 0.20060730666028448 1102 96-97 0.1407784273203454 1102 98-99 0.08333517696305748 1102 100-101 0.17721562888292652 2305 1 0.08441106464238146 2305 2 0.08702286384149005 2305 3 0.04980980491687603 2305 4 0.050815989896861424 2305 5 0.057097926044122005 2305 6 0.012818816892050222 2305 7 0.020814666432045215 2305 8 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-0.9894008974292632 1213 94-95 -0.9860632188116298 1213 96-97 -0.991821473327775 1213 98-99 -1.1016215309343416 1213 100-101 -0.9145211389664993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 9.0 9 45.0 10 71.0 11 74.0 12 130.0 13 379.0 14 1132.0 15 2699.0 16 5167.0 17 8523.0 18 12177.0 19 16291.0 20 21076.0 21 27059.0 22 34275.0 23 43070.0 24 53778.0 25 67147.0 26 85742.0 27 115636.0 28 172176.0 29 245340.0 30 222807.0 31 208579.0 32 268947.0 33 376164.0 34 569183.0 35 957455.0 36 1778749.0 37 2624305.0 38 653821.0 39 668.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.52068637884033 28.32489287014539 16.90855884465669 17.245861906357593 2 30.670761381860228 27.976106877245797 25.13536030056426 16.217771440329717 3 33.201856930670495 21.41683058931314 26.000762192187914 19.380550287828456 4 30.23571780506965 21.070989756234095 24.590556797020266 24.102735641675988 5 34.52402726989799 19.90338839354246 25.09352972164118 20.479054614918375 6 32.33048816962171 20.21259172748393 26.50881708348712 20.94810301940724 7 30.501257716960257 24.16229191445005 22.32780056075839 23.0086498078313 8 28.76094932317517 26.95005620182076 21.860466906716177 22.428527568287894 9 29.664902767817193 22.70996827595024 25.536261956010005 22.088867000222567 10-11 23.996488126195555 29.757461288287036 28.43050584875834 17.81554473675907 12-13 21.470168708114013 29.26019833246935 28.792853013458107 20.47677994595853 14-15 21.634563116581102 29.32220347532089 28.878689688027404 20.164543720070604 16-17 23.857214330849875 26.88878019612059 28.88113769210517 20.372867780924373 18-19 21.42755657626627 26.714931253671548 31.15717892522708 20.7003332448351 20-21 23.51064591733083 27.14261567799833 30.686019161344717 18.66071924332612 22-23 21.376215050785724 31.429896908599464 28.186279470298253 19.007608570316563 24-25 21.346228412225074 26.717456719465428 30.598736030616347 21.337578837693155 26-27 21.347365746704998 26.92369337182462 28.671753137526718 23.057187743943665 28-29 23.039981914632023 29.548947143225757 28.531942651279486 18.879128290862738 30-31 20.625519965994283 27.95649806431504 30.65072679756006 20.767255172130618 32-33 20.377656871669945 30.21855136015872 28.58554901643314 20.818242751738197 34-35 22.591982946748484 30.26082520790474 28.69490810104103 18.452283744305745 36-37 22.452907387225757 29.94020193984265 29.06234716936893 18.544543503562657 38-39 20.647568692393264 27.506714837862567 32.93086786366757 18.91484860607659 40-41 20.613044438891652 27.490205040300896 28.264026104649574 23.632724416157874 42-43 20.69411273595534 29.446054940316387 30.395081943978074 19.4647503797502 44-45 20.516542721267967 27.22449639502265 30.679816559565648 21.579144324143737 46-47 22.68813214834679 27.399822603149175 28.42026570331634 21.491779545187697 48-49 20.502166901995974 29.93598774526059 30.27904532070902 19.282800032034416 50-51 22.833744942330508 27.93436405024653 29.652920320193516 19.578970687229443 52-53 20.592445896910565 27.82464930344793 27.584883908182945 23.998020891458566 54-55 22.797356905283273 27.77772268737962 29.768000074663693 19.656920332673415 56-57 20.775537236018202 27.457449679980435 29.963074238927916 21.80393884507345 58-59 20.79278873541808 27.713133874106227 29.99798442553065 21.496092964945046 60-61 22.513173998037384 27.898724345053722 29.875330075447565 19.71277158146133 62-63 20.23657961755503 27.917540377331022 29.832104072588205 22.01377593252574 64-65 22.332688598839383 27.693222891197294 29.94788418858945 20.026204321373875 66-67 20.659320527328067 31.370430432655578 27.87896927673113 20.09127976328523 68-69 20.525377590861012 31.74834046813467 27.815936417809954 19.910345523194362 70-71 20.324089611274037 32.149772755808996 27.76122579768849 19.764911835228475 72-73 20.322706024034808 32.0055721292257 27.603089466430763 20.068632380308728 74-75 20.611568027092016 31.43828798851182 27.59109633328839 20.359047651107772 76-77 20.771150824468037 30.910360215019256 27.747998270603823 20.57049068990888 78-79 20.645759463621232 30.598600100230342 28.049108085844825 20.7065323503036 80-81 20.366076486789435 30.704496604144094 27.895892306988735 21.03353460207774 82-83 20.221734414701352 30.61224263636298 27.783018636823982 21.383004312111684 84-85 20.402322568406987 30.35208984900319 27.994517139829444 21.251070442760383 86-87 20.409920629943493 30.110492709219628 28.520944786897335 20.95864187393955 88-89 20.607931127258183 30.48267879198286 28.160074194913086 20.749315885845867 90-91 20.814780282385772 30.10448358778031 27.802848360154837 21.27788776967908 92-93 20.56153598034232 30.19940441597616 27.6602251259997 21.57883447768182 94-95 20.227583364701154 30.204294945109616 27.597687330068275 21.970434360120947 96-97 20.124826171688582 30.573853374900438 27.68851926865975 21.61280118475123 98-99 20.460869065981267 30.284117814407978 27.59336068112286 21.661652438487895 100-101 20.667638362194083 30.263658649497998 27.173195530371686 21.89550745793623 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29.0 1 20.0 2 14.5 3 39.5 4 52.0 5 41.5 6 38.0 7 35.0 8 35.5 9 43.5 10 55.5 11 66.0 12 66.5 13 78.0 14 104.5 15 138.5 16 176.5 17 241.5 18 381.5 19 587.5 20 903.0 21 1385.5 22 2323.0 23 3789.5 24 5623.5 25 7983.0 26 11575.5 27 16577.5 28 22455.5 29 29305.0 30 37149.5 31 46241.0 32 63212.0 33 92665.5 34 135594.0 35 198911.0 36 276524.5 37 366654.0 38 460353.5 39 519617.5 40 566182.5 41 577079.0 42 545992.0 43 538415.0 44 526050.0 45 512904.5 46 525932.0 47 498483.0 48 412528.5 49 329576.0 50 267837.0 51 219312.0 52 176774.0 53 140103.5 54 113852.5 55 91279.0 56 67721.5 57 47389.5 58 33532.0 59 24310.0 60 17542.0 61 12265.5 62 8168.5 63 5442.0 64 3673.0 65 2520.0 66 1799.0 67 1261.5 68 931.5 69 706.5 70 507.5 71 357.0 72 267.5 73 198.0 74 137.5 75 111.0 76 99.0 77 87.5 78 72.0 79 52.0 80 34.0 81 21.0 82 19.5 83 20.0 84 13.0 85 11.5 86 11.5 87 7.5 88 3.5 89 3.5 90 2.5 91 0.0 92 0.0 93 1.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16218972932139275 2 0.0 3 4.0827391586944383E-4 4 0.019912102125404015 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.190911216054357E-4 12-13 0.0 14-15 0.0 16-17 0.0010848421193102363 18-19 0.0 20-21 0.0 22-23 4.7243124550607065E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.57375223325832E-4 38-39 6.590707499035308E-4 40-41 0.0047826373001849136 42-43 0.003085384307070511 44-45 9.79857398086665E-4 46-47 0.004321871023703684 48-49 0.007529737505535029 50-51 0.004928449412995428 52-53 0.0015106134887169422 54-55 0.010568461936506174 56-57 0.01819151919423993 58-59 0.02237924307415794 60-61 0.011215867717384862 62-63 5.132586370930151E-4 64-65 0.013869648170536248 66-67 0.03346096364775713 68-69 0.019358016096724058 70-71 0.03546150583551741 72-73 0.029430716849674476 74-75 0.02888829579001936 76-77 0.037456215538765256 78-79 0.016558423530762156 80-81 0.012271547414132998 82-83 9.098675839376176E-4 84-85 0.010177685474173992 86-87 0.020396198339934928 88-89 0.020273716165174094 90-91 0.024443942591554843 92-93 0.04208720824162723 94-95 0.027086058075681384 96-97 0.028439194482562973 98-99 0.03571813515406391 100-101 0.016377616510877116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8572676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.87132014080958 #Duplication Level Percentage of deduplicated Percentage of total 1 88.51877821831677 40.6047321412573 2 5.335258989956748 4.894707463248767 3 1.8602338814225485 2.5599415173454356 4 0.8921675350202327 1.6369961047260013 5 0.5392251130893582 1.2367483895243099 6 0.43338880282318604 1.192806991184674 7 0.2883056776790848 0.9257473427461251 8 0.2432770194595505 0.8927550434024801 9 0.17849679233457505 0.7369095154758207 >10 1.366193778664127 12.554729818645487 >50 0.1991243421702689 6.394312027763894 >100 0.12299723547070007 10.461746095336057 >500 0.012171281275110744 3.9461739107510123 >1k 0.010279168617621345 8.10105680758616 >5k 7.66972509714972E-5 0.18776321668003312 >10k+ 2.54664491674299E-5 3.672873614326424 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 314410 3.667582911100338 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002507968340340869 0.0 0.0 2 0.002916242256210313 0.0 0.0 3 0.003102881760607773 0.0 1.1664969024841252E-5 4 0.0033245161720797567 0.0 1.1664969024841252E-5 5 0.0038377748091727717 0.0 2.3329938049682503E-5 6 0.004234383756017374 0.0 2.3329938049682503E-5 7 0.0047476423931103896 0.0 2.3329938049682503E-5 8 0.005587520162898959 0.0 2.3329938049682503E-5 9 0.006287418304389434 0.0 2.3329938049682503E-5 10-11 0.009816071434403913 0.0 2.3329938049682503E-5 12-13 0.013163917544533353 0.0 2.3329938049682503E-5 14-15 0.017328311486401682 0.0 2.3329938049682503E-5 16-17 0.021918476797676712 0.0 2.3329938049682503E-5 18-19 0.02779178870168428 0.0 4.082739158694438E-5 20-21 0.03460413061219157 0.0 4.6659876099365006E-5 22-23 0.04286292868177918 0.0 4.6659876099365006E-5 24-25 0.05238737589056206 0.0 5.832484512420626E-5 26-27 0.06314247733146569 0.0 6.99898141490475E-5 28-29 0.07509323809741555 0.0 7.582229866146814E-5 30-31 0.11072388598379315 0.0 9.331975219873001E-5 32-33 0.1260924826740215 0.0 9.331975219873001E-5 34-35 0.14120445004570334 0.0 9.331975219873001E-5 36-37 0.15681801108545335 0.0 9.331975219873001E-5 38-39 0.17414632257185503 0.0 9.331975219873001E-5 40-41 0.19223868952938383 0.0 9.331975219873001E-5 42-43 0.21175418270794324 0.0 1.1664969024841252E-4 44-45 0.23182959439969503 0.0 1.2248217476083315E-4 46-47 0.2526807265315988 0.0 1.3414714378567438E-4 48-49 0.27459920332927545 0.0 1.39979628298095E-4 50-51 0.2970309387640452 0.0 1.5164459732293627E-4 52-53 0.3198359532076098 0.0 1.808070198850394E-4 54-55 0.34306673902058116 0.0 1.8663950439746002E-4 56-57 0.36807643260984085 0.0 1.8663950439746002E-4 58-59 0.39388517657730215 0.0 1.8663950439746002E-4 60-61 0.42133868117726603 0.0 1.8663950439746002E-4 62-63 0.4500928298234997 0.0 1.8663950439746002E-4 64-65 0.4807192059982204 0.0 1.8663950439746002E-4 66-67 0.5126578911882358 0.0 1.9830447342230128E-4 68-69 0.546008037630257 0.0 1.9830447342230128E-4 70-71 0.5823444161426374 0.0 2.041369579347219E-4 72-73 0.621276250263045 0.0 2.0996944244714252E-4 74-75 0.6633926209272344 5.832484512420626E-6 2.274668959844044E-4 76-77 0.7082852542193359 1.1664969024841252E-5 2.3329938049682504E-4 78-79 0.7567823629401135 1.1664969024841252E-5 2.3329938049682504E-4 80-81 0.8090589216249395 1.1664969024841252E-5 2.449643495216663E-4 82-83 0.8665672189174068 1.1664969024841252E-5 2.507968340340869E-4 84-85 0.9285023719548016 1.1664969024841252E-5 2.5662931854650756E-4 86-87 0.9967774356571973 1.1664969024841252E-5 2.5662931854650756E-4 88-89 1.071870673754613 1.1664969024841252E-5 2.5662931854650756E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 47630 0.0 71.1636 3 CGGAAGA 47565 0.0 71.13103 4 GAAGAGC 48800 0.0 69.34876 6 GATCGGA 49075 0.0 69.1388 1 ATCGGAA 50685 0.0 66.90238 2 GGAAGAG 55315 0.0 61.292477 5 ACGTTCG 40 5.3488644E-4 47.57412 1 AGAGCAC 76400 0.0 44.482555 8 GAGCACA 76480 0.0 44.423603 9 AAGAGCA 76850 0.0 44.14175 7 GCCGTCT 38695 0.0 43.573135 50-51 CGTCTTC 38785 0.0 43.406433 52-53 ATGCCGT 38805 0.0 43.359882 48-49 TGCCGTC 38785 0.0 43.22095 50-51 CCGTCTT 38765 0.0 43.189873 52-53 TATGCCG 38905 0.0 43.065304 48-49 TCTCGTA 38650 0.0 42.937004 42-43 TCGTATG 39390 0.0 42.842033 44-45 CGTATGC 39140 0.0 42.80017 46-47 CTCGTAT 38855 0.0 42.63737 44-45 >>END_MODULE