##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_30423-1.3410000003574b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2681201 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.672160722004804 33.0 33.0 33.0 33.0 33.0 2 32.70007768906546 33.0 33.0 33.0 33.0 33.0 3 32.736087671159304 33.0 33.0 33.0 33.0 33.0 4 36.65192277639759 37.0 37.0 37.0 37.0 37.0 5 36.66023770690821 37.0 37.0 37.0 37.0 37.0 6 36.566702757458316 37.0 37.0 37.0 37.0 37.0 7 36.45278216739439 37.0 37.0 37.0 37.0 37.0 8 36.40666701228293 37.0 37.0 37.0 37.0 37.0 9 36.4364614961728 37.0 37.0 37.0 37.0 37.0 10-11 36.367228156337404 37.0 37.0 37.0 37.0 37.0 12-13 36.34761269296856 37.0 37.0 37.0 37.0 37.0 14-15 38.19421669617459 40.0 37.0 40.0 37.0 40.0 16-17 38.1953682323705 40.0 37.0 40.0 37.0 40.0 18-19 38.21083424927859 40.0 37.0 40.0 37.0 40.0 20-21 38.20680657660504 40.0 37.0 40.0 37.0 40.0 22-23 37.971350898347424 40.0 37.0 40.0 33.0 40.0 24-25 38.06365785332767 40.0 37.0 40.0 35.0 40.0 26-27 37.982924443187954 40.0 37.0 40.0 35.0 40.0 28-29 37.78308060454998 40.0 37.0 40.0 33.0 40.0 30-31 37.76053156775639 40.0 37.0 40.0 33.0 40.0 32-33 37.65000255482525 40.0 37.0 40.0 33.0 40.0 34-35 37.636497972363884 40.0 37.0 40.0 33.0 40.0 36-37 37.51762288616184 40.0 37.0 40.0 33.0 40.0 38-39 37.200788378044024 40.0 37.0 40.0 33.0 40.0 40-41 37.056373058192946 38.5 37.0 40.0 33.0 40.0 42-43 36.81747750355158 37.0 37.0 40.0 33.0 40.0 44-45 36.64813175140543 37.0 37.0 40.0 33.0 40.0 46-47 36.66429521695688 37.0 37.0 40.0 33.0 40.0 48-49 36.32042096060683 37.0 37.0 40.0 33.0 40.0 50-51 36.29072419412047 37.0 37.0 40.0 33.0 40.0 52-53 36.28622658278883 37.0 37.0 40.0 33.0 40.0 54-55 35.85957897225907 37.0 35.0 40.0 30.0 40.0 56-57 35.66272465212418 37.0 35.0 40.0 27.0 40.0 58-59 35.49987356412295 37.0 33.0 40.0 27.0 40.0 60-61 35.16855151851726 37.0 33.0 38.5 27.0 40.0 62-63 34.951097474601866 37.0 33.0 37.0 27.0 40.0 64-65 34.67408709753577 37.0 33.0 37.0 27.0 40.0 66-67 33.8965284587019 37.0 33.0 37.0 27.0 40.0 68-69 33.82402867222562 37.0 33.0 37.0 27.0 37.0 70-71 33.50413620612554 37.0 33.0 37.0 27.0 37.0 72-73 32.64769314199122 37.0 33.0 37.0 22.0 37.0 74-75 31.61446269787308 35.0 33.0 37.0 18.5 37.0 76-77 28.97567209619868 33.0 27.0 33.0 15.0 37.0 78-79 31.199266485429476 33.0 33.0 37.0 15.0 37.0 80-81 31.567755643832747 35.0 33.0 37.0 15.0 37.0 82-83 31.330654434337447 33.0 33.0 37.0 15.0 37.0 84-85 31.208717660481256 33.0 33.0 37.0 15.0 37.0 86-87 31.122886161835687 33.0 33.0 37.0 15.0 37.0 88-89 30.91039407340218 33.0 33.0 37.0 15.0 37.0 90-91 30.913787701854503 33.0 33.0 37.0 15.0 37.0 92-93 30.72581932499652 33.0 33.0 37.0 15.0 37.0 94-95 30.489231504836823 33.0 30.0 37.0 15.0 37.0 96-97 30.37533161445188 33.0 27.0 37.0 15.0 37.0 98-99 30.24999599060272 33.0 27.0 37.0 15.0 37.0 100-101 28.67070447161552 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.16695330004460374 2306 2 0.17444658554883574 2306 3 0.1198617544799987 2306 4 0.15226130902397017 2306 5 0.1403649762358441 2306 6 0.16236999996717572 2306 7 0.197392831815705 2306 8 0.24753286482697234 2306 9 0.25148090066994655 2306 10-11 0.24752324699678496 2306 12-13 0.3354415921792082 2306 14-15 0.538439891980353 2306 16-17 0.48915905403507054 2306 18-19 0.4480360669464929 2306 20-21 0.5003577957237724 2306 22-23 0.41926902898497787 2306 24-25 0.49363998792270536 2306 26-27 0.4322270194834843 2306 28-29 0.4599527548802982 2306 30-31 0.5254315359171997 2306 32-33 0.49028801584161386 2306 34-35 0.6992635046811557 2306 36-37 0.5155188799289547 2306 38-39 0.6321095011933906 2306 40-41 0.6428564276941984 2306 42-43 0.7496575339630382 2306 44-45 0.534660189044736 2306 46-47 0.3412093038674229 2306 48-49 0.1382688032749968 2306 50-51 0.22805472801281468 2306 52-53 0.19506726611189862 2306 54-55 -0.11701616757962796 2306 56-57 0.0758086867193839 2306 58-59 0.21963432000890037 2306 60-61 0.05332387435957742 2306 62-63 0.020750089017930406 2306 64-65 -0.11019632313240635 2306 66-67 0.0249606977682717 2306 68-69 0.37827221390147514 2306 70-71 0.2954728149170691 2306 72-73 0.24511253380516962 2306 74-75 0.32939368337325803 2306 76-77 0.014368132480516493 2306 78-79 0.24320881514577763 2306 80-81 0.38213617522288246 2306 82-83 0.5968487196329129 2306 84-85 0.4150958359041752 2306 86-87 0.2861837962526579 2306 88-89 0.7885497884834365 2306 90-91 0.5343513966294999 2306 92-93 0.596420741887556 2306 94-95 0.5443182070474109 2306 96-97 0.4318164661006527 2306 98-99 0.4682532857845345 2306 100-101 0.3712131497087867 2307 1 0.1933001820131537 2307 2 0.15830084960337842 2307 3 0.13143031132539562 2307 4 0.2005055442741579 2307 5 0.16672954962626108 2307 6 0.14358307082513022 2307 7 0.20312158639131894 2307 8 0.17827122040114318 2307 9 0.22834351480342718 2307 10-11 0.20635326699898116 2307 12-13 0.2538004433472594 2307 14-15 0.46627236346926537 2307 16-17 0.4386956340437109 2307 18-19 0.4458768969517948 2307 20-21 0.43208061088079575 2307 22-23 0.3999022294693404 2307 24-25 0.36697961395870493 2307 26-27 0.4590631373105296 2307 28-29 0.4391914630581155 2307 30-31 0.44417061656749013 2307 32-33 0.5190128970845436 2307 34-35 0.44835328102620053 2307 36-37 0.5096992186625258 2307 38-39 0.47338832426202515 2307 40-41 0.4753963688008156 2307 42-43 0.4482765415722483 2307 44-45 0.3304184393032372 2307 46-47 0.38784348364003307 2307 48-49 0.20064521848667738 2307 50-51 0.23979815775894764 2307 52-53 0.2559073854663936 2307 54-55 0.0640709131370798 2307 56-57 0.21911628039477904 2307 58-59 0.4082463789719597 2307 60-61 0.16371861897302153 2307 62-63 0.1556968528077789 2307 64-65 0.19507691815653772 2307 66-67 0.1404296143483137 2307 68-69 0.12851125222844217 2307 70-71 0.28161662113667063 2307 72-73 0.2660027008312369 2307 74-75 0.15360164522074982 2307 76-77 -0.17675842188100432 2307 78-79 0.22310196984474118 2307 80-81 0.3637108315261912 2307 82-83 0.48314146040703676 2307 84-85 0.47677956390591447 2307 86-87 0.3316744297334857 2307 88-89 0.5521054322239252 2307 90-91 0.3436001725872373 2307 92-93 0.5354781483741 2307 94-95 0.48279043573380775 2307 96-97 0.3993001770641875 2307 98-99 0.2945883008916468 2307 100-101 0.3640053922652555 2201 1 0.15688085312888234 2201 2 0.13674611736886533 2201 3 0.14114832149692091 2201 4 0.15399819094066203 2201 5 0.15765979806670316 2201 6 0.1549091891912795 2201 7 0.05842759646893825 2201 8 0.2120214845065007 2201 9 0.19544612427110053 2201 10-11 0.22285144867358753 2201 12-13 0.23352142003556509 2201 14-15 0.39531538970053504 2201 16-17 0.3376314495050252 2201 18-19 0.2729752540563837 2201 20-21 0.3527635019754882 2201 22-23 0.4064036230919683 2201 24-25 0.4618974798593456 2201 26-27 0.3734803288087889 2201 28-29 0.37201719833811353 2201 30-31 0.32706523265967036 2201 32-33 0.5068703196723234 2201 34-35 0.43472611136064643 2201 36-37 0.5024479258801264 2201 38-39 0.6429354289203175 2201 40-41 0.5498799494505917 2201 42-43 0.5283363467638651 2201 44-45 0.4877734801408593 2201 46-47 0.5295385438560558 2201 48-49 0.31648022893351424 2201 50-51 0.24850016261208907 2201 52-53 0.27959630243166345 2201 54-55 0.2143004447048682 2201 56-57 0.1921581925816156 2201 58-59 0.4060644418578434 2201 60-61 0.13736223786737867 2201 62-63 0.19557422788861345 2201 64-65 0.33017841464687336 2201 66-67 0.18303501106124287 2201 68-69 0.4547149857712043 2201 70-71 0.15805212714119676 2201 72-73 0.39544047120576664 2201 74-75 0.30551691595275443 2201 76-77 0.0652910730641807 2201 78-79 0.3585562737483805 2201 80-81 0.5487032978540896 2201 82-83 0.6183425966469862 2201 84-85 0.5729660692424403 2201 86-87 0.5482016096431224 2201 88-89 0.8275827959935782 2201 90-91 0.5273662830142207 2201 92-93 0.5881649002089695 2201 94-95 0.47282312610842325 2201 96-97 0.5423564469787969 2201 98-99 0.5926588111538749 2201 100-101 0.5210944603308079 1101 1 0.09715476155277969 1101 2 0.1206747037370306 1101 3 0.09961237624514041 1101 4 0.1127540158869138 1101 5 0.08672512949254951 1101 6 0.11962904797032081 1101 7 0.1875406150669363 1101 8 0.15240519891890614 1101 9 0.18966928350442203 1101 10-11 0.16668777709598004 1101 12-13 0.16925145207800085 1101 14-15 0.294736764429679 1101 16-17 0.3522434892610633 1101 18-19 0.2786626813752804 1101 20-21 0.25595488230997177 1101 22-23 0.2968209067637133 1101 24-25 0.31606362364034624 1101 26-27 0.3289215815591717 1101 28-29 0.274086988932865 1101 30-31 0.453987360344172 1101 32-33 0.4590017061977747 1101 34-35 0.34969705983340305 1101 36-37 0.41852111732320907 1101 38-39 0.4807132903597946 1101 40-41 0.5170354096318022 1101 42-43 0.5709708747863615 1101 44-45 0.6001363795209258 1101 46-47 0.5803008065705413 1101 48-49 0.4872410903144342 1101 50-51 0.3023568626597708 1101 52-53 0.3513811830882432 1101 54-55 0.4799911975765596 1101 56-57 0.5642640204560223 1101 58-59 0.6259326328407937 1101 60-61 0.5877226106639455 1101 62-63 0.618792128983749 1101 64-65 0.4550157972067552 1101 66-67 0.3726882542072616 1101 68-69 0.5846970403353708 1101 70-71 0.371203328941597 1101 72-73 0.459979202392347 1101 74-75 0.26892642326896876 1101 76-77 -0.04538133697857205 1101 78-79 0.2314961042497643 1101 80-81 0.3766603208359598 1101 82-83 0.4712893710699326 1101 84-85 0.42193525245320984 1101 86-87 0.40630089935524083 1101 88-89 0.6206047822897922 1101 90-91 0.33667929491928206 1101 92-93 0.3478827301916496 1101 94-95 0.33529949167661144 1101 96-97 0.25303858269481694 1101 98-99 0.083482690714483 1101 100-101 0.18063733277180916 2304 1 0.10467495699528939 2304 2 0.10572582012576959 2304 3 0.10350377626386376 2304 4 0.11735023472123629 2304 5 0.1006504084833324 2304 6 0.19066082440315313 2304 7 0.15711848988498645 2304 8 0.15702823151251977 2304 9 0.08206268817087192 2304 10-11 0.08652889493180993 2304 12-13 0.13921982886664352 2304 14-15 0.2474151610519968 2304 16-17 0.34277440169498874 2304 18-19 0.29955166389952126 2304 20-21 0.3005395669716435 2304 22-23 0.3683985347249319 2304 24-25 0.31725853838962337 2304 26-27 0.2417068501649382 2304 28-29 0.29519175837709355 2304 30-31 0.3540935513432544 2304 32-33 0.16725672632937716 2304 34-35 0.3442742962286047 2304 36-37 0.347347611019849 2304 38-39 0.4208811530052472 2304 40-41 0.34548033927760713 2304 42-43 0.42974408145659737 2304 44-45 0.5239233761245217 2304 46-47 0.47266995116905974 2304 48-49 0.3762915001818996 2304 50-51 0.34512736241717334 2304 52-53 0.3197428623956782 2304 54-55 0.3311407843041678 2304 56-57 0.2948202144344165 2304 58-59 0.5429691591395525 2304 60-61 0.3523241922501228 2304 62-63 0.3771448278099001 2304 64-65 0.43180167869844865 2304 66-67 0.26563921482082975 2304 68-69 0.397256732870936 2304 70-71 0.32304922218028054 2304 72-73 0.06232391667684567 2304 74-75 0.09518603153271599 2304 76-77 0.029317114633233388 2304 78-79 0.3572185860972752 2304 80-81 0.3849717349851147 2304 82-83 0.531587344290223 2304 84-85 0.3631433187129893 2304 86-87 0.37254939906156537 2304 88-89 0.7546448497072404 2304 90-91 0.6073848372807653 2304 92-93 0.4937763944833833 2304 94-95 0.3925119673101385 2304 96-97 0.5236911359605649 2304 98-99 0.4916151336607584 2304 100-101 0.643016138835975 2202 1 0.05331608610184446 2202 2 0.08233113031683814 2202 3 0.0349061310258989 2202 4 0.05924030897546828 2202 5 0.0654874361029556 2202 6 0.03006900158149506 2202 7 0.09597943363233696 2202 8 0.11473648423486793 2202 9 0.10373644860423781 2202 10-11 0.11564834659285594 2202 12-13 0.13561029926628976 2202 14-15 0.16343417603182075 2202 16-17 0.2260165294564942 2202 18-19 0.17925796353285506 2202 20-21 0.26408958263321125 2202 22-23 0.3126406072103336 2202 24-25 0.2752827861739746 2202 26-27 0.1952150402410382 2202 28-29 0.3179793323088873 2202 30-31 0.35537964203291494 2202 32-33 0.41613830648893924 2202 34-35 0.24142849813379286 2202 36-37 0.31739717525989164 2202 38-39 0.3577051723150291 2202 40-41 0.40708463607342793 2202 42-43 0.4431153710481013 2202 44-45 0.4446159857093974 2202 46-47 0.46518372413166986 2202 48-49 0.4542575238032214 2202 50-51 0.3520755687075976 2202 52-53 0.406838655884151 2202 54-55 0.4307289773283287 2202 56-57 0.44623159762679165 2202 58-59 0.505637520940077 2202 60-61 0.4022302228730865 2202 62-63 0.40082879589716214 2202 64-65 0.32112049284176436 2202 66-67 0.3613414977239344 2202 68-69 0.3522168419491152 2202 70-71 0.14335029269415855 2202 72-73 -0.023826655430838173 2202 74-75 0.044191061658192865 2202 76-77 0.021675193235854096 2202 78-79 0.35921589599353965 2202 80-81 0.4531105885587152 2202 82-83 0.3584985155908704 2202 84-85 0.4199045075177352 2202 86-87 0.44303011691390637 2202 88-89 0.6193667187765755 2202 90-91 0.43570278543178276 2202 92-93 0.581582485910058 2202 94-95 0.5530604905168914 2202 96-97 0.46650984876434975 2202 98-99 0.4690891999552349 2202 100-101 0.47079883004522927 1102 1 0.04745271664717876 1102 2 -0.0016802453300499565 1102 3 0.0245132431281192 1102 4 0.08922748316589235 1102 5 0.017559227452089488 1102 6 -0.03937487596105882 1102 7 0.11898641399208998 1102 8 0.0947516163672546 1102 9 0.07695456535062561 1102 10-11 0.07874436096838622 1102 12-13 0.062178391373194586 1102 14-15 0.13529936067867965 1102 16-17 0.19784131197356913 1102 18-19 0.12051948892480624 1102 20-21 0.12746998818099797 1102 22-23 0.1561516269534522 1102 24-25 0.05052839296087086 1102 26-27 0.08071366225463805 1102 28-29 0.09175171318067754 1102 30-31 0.10329308358090827 1102 32-33 0.16918547327922084 1102 34-35 -0.0029232509382808303 1102 36-37 0.15454589657811368 1102 38-39 0.3057344277709788 1102 40-41 0.18533509941202198 1102 42-43 0.21993558240018984 1102 44-45 0.054777660597515876 1102 46-47 0.121658269130684 1102 48-49 0.20489308995569644 1102 50-51 0.3174199989380142 1102 52-53 0.18373228794435903 1102 54-55 0.17439961452051733 1102 56-57 0.0750418005334268 1102 58-59 0.2611591729972744 1102 60-61 0.22648195385961856 1102 62-63 0.21377058530500648 1102 64-65 0.1722875917365556 1102 66-67 0.21585326245909187 1102 68-69 0.13018108896790181 1102 70-71 0.03710115341714726 1102 72-73 -0.0432809031299044 1102 74-75 0.056567304210343394 1102 76-77 0.04992922390828625 1102 78-79 0.22405700030736142 1102 80-81 0.2492645878717923 1102 82-83 0.32711775828737544 1102 84-85 0.23994520830802202 1102 86-87 0.13535387817796973 1102 88-89 0.4124180502217101 1102 90-91 0.09023346533242815 1102 92-93 0.2564897767108185 1102 94-95 0.3561965917694394 1102 96-97 0.2497407249513195 1102 98-99 0.23095216124053763 1102 100-101 0.20300571549731217 2305 1 0.014408893839352288 2305 2 0.049511254779780245 2305 3 0.009616648722371224 2305 4 0.07274627202192363 2305 5 0.04486820407397829 2305 6 0.06642335489440399 2305 7 0.03038688599539796 2305 8 0.06739625341969457 2305 9 0.06480613629088339 2305 10-11 0.06722918007069012 2305 12-13 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-0.8004179689955926 1213 96-97 -0.8138395290743432 1213 98-99 -0.8351503657412351 1213 100-101 -0.8399168570197268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 10.0 10 26.0 11 13.0 12 35.0 13 84.0 14 263.0 15 786.0 16 1566.0 17 2647.0 18 3687.0 19 4773.0 20 6510.0 21 8783.0 22 11982.0 23 16385.0 24 21808.0 25 28184.0 26 37699.0 27 54941.0 28 86402.0 29 104256.0 30 70456.0 31 58014.0 32 76756.0 33 109356.0 34 168899.0 35 305465.0 36 591263.0 37 777958.0 38 132166.0 39 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51823382662679 21.693318758923773 21.39359946324731 12.394847951202127 2 31.47548430721904 27.102928874038163 29.772366935563575 11.649219883179217 3 38.22666867075117 14.850116981595118 32.03427133687977 14.888943010773945 4 31.504266649258117 14.241077340595185 30.00110046909827 24.25355554104843 5 38.83714797958079 13.057954252590537 32.63436795674774 15.47052981108093 6 33.829354830167524 13.748764079977594 36.78269551592738 15.639185573927506 7 33.78060801857078 23.509240821557206 25.410702144300256 17.299449015571753 8 30.053211228848564 33.33964891106634 19.975637783217298 16.63150207686779 9 38.308429692514665 23.732610871023844 20.432858260160277 17.526101176301218 10-11 30.22197622844799 28.805290789008453 25.16783604879628 15.804896933747278 12-13 25.420492533010393 27.185373271157214 24.832938671886218 22.56119552394617 14-15 24.230522068282088 28.000213337232083 24.675397331270577 23.093867263215255 16-17 27.860308980262193 24.892691617050662 25.44010097067535 21.806898432011792 18-19 22.18904886280439 25.036634702135345 29.932836068612534 22.841480366447723 20-21 26.10233249950302 25.6967866265901 30.222258607243546 17.978622266663336 22-23 22.085752967898582 33.56849011073441 25.79503877009835 18.550718151268654 24-25 22.73833629034153 23.040719438788813 31.064511761706786 23.156432509162872 26-27 20.62775226475001 23.1289821240556 27.54450337740438 28.69876223379001 28-29 24.57702723518304 29.380844628955455 25.438264419564216 20.603863716297287 30-31 18.557691124238726 25.980334931995024 30.470058007586896 24.991915936179346 32-33 19.12297884418214 28.75935075363615 26.700758354185304 25.416912047996405 34-35 20.038874370105038 24.16583090935741 30.702584401542442 25.09271031899511 36-37 23.576004515922936 26.555756524320735 26.426485060026756 23.441753899729576 38-39 23.6689452636934 30.807811629348546 24.259225968248334 21.26401713870972 40-41 24.640350425686346 29.941866753795672 24.0874766232439 21.330306197274084 42-43 21.23698470548458 30.2932034674257 28.989552704471578 19.48025912261814 44-45 19.066973546702233 26.992090789943823 28.86542780822459 25.07550785512935 46-47 24.189971065899442 26.412124908036557 25.37090756846323 24.02699645760077 48-49 20.011853748318963 29.720961347194535 30.398281094595557 19.86890380989094 50-51 23.876514503878294 25.466342617665443 28.549918017989796 22.10722486046647 52-53 19.057928176744067 26.359213252448093 24.766976295752034 29.815882275055806 54-55 24.238851309703364 26.260163678184245 29.854306236470418 19.646678775641973 56-57 20.23581505221113 25.62494346164858 28.478132131409584 25.66110935473071 58-59 19.021382804559522 24.884796752653383 29.496190855105137 26.597629587681958 60-61 22.759376867372445 24.856466433362453 31.2244256232781 21.159731075986997 62-63 18.861317342399968 24.904575810853853 30.93451927617107 25.29958757057511 64-65 24.53556905508341 26.493522819107255 29.344426147902823 19.626481977906508 66-67 20.28076376638998 34.751497595787924 24.632577894381814 20.335160743440277 68-69 19.705414242918636 32.90303820355995 26.666802204441076 20.724745349080337 70-71 19.315688402647375 35.78375974399317 25.386056701617505 19.51449515174195 72-73 21.215957128898708 35.10545226900243 24.637340523286202 19.04125007881266 74-75 22.523459080171257 32.93717532976274 24.902123255678603 19.637242334387402 76-77 21.702441784476967 31.244894254757543 25.24159187878926 21.81107208197623 78-79 21.672131227038914 32.29306183305956 25.196917215780534 20.837889724120988 80-81 21.185866142209562 32.38287606530349 25.18574677786202 21.24551101462493 82-83 20.546155458533523 31.462178191824634 26.04952002440718 21.942146325234653 84-85 21.09419032176605 30.72755166783977 25.974170089927984 22.204087920466197 86-87 19.299516588330114 31.367368434600092 27.248026539985215 22.08508843708458 88-89 18.986733566175957 31.643032674130687 27.25599787364013 22.114235886053223 90-91 20.47445840934442 30.475540284943648 26.02587921045465 23.024122095257283 92-93 20.56448934847613 30.757595092097787 25.140667127845894 23.537248431580178 94-95 20.89493744626613 31.79254421496645 24.980642282626963 22.33187605614046 96-97 20.33335944844876 32.97292608676187 23.917491158168062 22.7762233066213 98-99 20.513227097683355 30.70723423100909 25.589756724826334 23.189781946481215 100-101 21.741802456954797 31.438949625245847 24.925193718566486 21.894054199232865 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 249.0 1 165.5 2 116.5 3 254.0 4 283.0 5 199.5 6 200.0 7 211.5 8 229.0 9 235.5 10 219.0 11 219.0 12 216.0 13 208.0 14 216.5 15 238.5 16 244.0 17 242.5 18 267.5 19 286.0 20 311.5 21 356.0 22 386.5 23 424.5 24 513.5 25 650.5 26 821.5 27 1040.0 28 1375.5 29 1782.5 30 2403.5 31 3494.0 32 5799.0 33 9953.0 34 16660.5 35 28475.5 36 46498.5 37 70276.0 38 98028.5 39 119902.5 40 135218.5 41 144232.0 42 177297.5 43 218721.0 44 210785.5 45 184513.0 46 177732.5 47 165286.0 48 136618.0 49 113936.0 50 96592.0 51 87597.0 52 85540.0 53 85895.0 54 71832.0 55 43638.5 56 27949.0 57 22479.5 58 19526.0 59 15816.5 60 11957.0 61 9316.0 62 6892.0 63 4908.0 64 3597.5 65 2545.5 66 1853.5 67 1445.0 68 1121.5 69 829.0 70 654.5 71 529.0 72 314.0 73 161.0 74 112.5 75 76.0 76 49.0 77 32.0 78 22.0 79 13.5 80 9.0 81 8.5 82 8.0 83 3.5 84 1.0 85 2.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16272558454215108 2 0.0 3 3.729671889574858E-4 4 0.019618074139163756 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.8485734564473164E-4 12-13 0.0 14-15 0.0 16-17 9.883630507373374E-4 18-19 0.0 20-21 0.0 22-23 4.102639078532344E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.018794562585945E-4 38-39 7.086376590192231E-4 40-41 0.004438309548594081 42-43 0.0028531989955247667 44-45 8.578245346022175E-4 46-47 0.004046694000188721 48-49 0.007179618387431603 50-51 0.004662089861968572 52-53 0.0013426818802469492 54-55 0.010051465742404243 56-57 0.01859241436953067 58-59 0.022844240323646008 60-61 0.011002532074245832 62-63 5.594507834362287E-4 64-65 0.013669247475291857 66-67 0.03375353060065247 68-69 0.019450238904132888 70-71 0.03580485013991864 72-73 0.029613594803224376 74-75 0.02942711120874563 76-77 0.0376510377252582 78-79 0.016503798111368748 80-81 0.012438455751732153 82-83 8.578245346022175E-4 84-85 0.010368487853018107 86-87 0.021128591254441574 88-89 0.021016701097754328 90-91 0.0250260983790473 92-93 0.04158584156875967 94-95 0.02780470393678057 96-97 0.028382803079664672 98-99 0.035972685374949506 100-101 0.016541094830264495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2681201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.957343589717574 #Duplication Level Percentage of deduplicated Percentage of total 1 83.18618523673638 26.58409503528276 2 7.509822232794168 4.799879387822066 3 2.3834464963990807 2.2850585583940193 4 1.3939831575614081 1.7819199489787736 5 0.9495518322138077 1.517257707915125 6 0.6913749481510743 1.3256704060432234 7 0.465084911132173 1.0404014812410882 8 0.34952345481517627 0.8935872910554973 9 0.29834763768888073 0.858095817012372 >10 2.1743230432293634 13.958201257672842 >50 0.3079080556981467 6.986987017391294 >100 0.25728435203455763 16.347241205497234 >500 0.022812038211268197 4.822422315771302 >1k 0.009298617830550433 4.746733209791973 >5k 4.684380018018968E-4 0.9493621421796119 >10k+ 5.855475020664587E-4 11.103087217950812 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 216780 8.08518272222038 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 29296 1.0926446767698506 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 27296 1.0180512389783534 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12590 0.46956569089747463 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10713 0.39955974953015455 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9314 0.3473816397950023 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5518 0.20580329486674068 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5443 0.20300604094955957 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5092 0.1899148926171518 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 3675 0.13706544194187603 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3371 0.12572723939756847 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3121 0.11640305967363133 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0038042653273663556 0.0 0.0 2 0.00421452923521959 0.0 0.0 3 0.004624793143072825 0.0 0.0 4 0.004885870175343064 0.0 0.0 5 0.0057809914288410305 0.0 0.0 6 0.0066015192445475 0.0 0.0 7 0.007608530654732711 0.0 0.0 8 0.008615542064917924 0.0 0.0 9 0.009659850193998884 0.0 0.0 10-11 0.015030577714986679 0.0 0.0 12-13 0.01999104132812124 0.0 0.0 14-15 0.025790681116410148 0.0 0.0 16-17 0.031385188950772434 0.0 0.0 18-19 0.03804265327366356 0.0 0.0 20-21 0.04387958978084821 0.0 0.0 22-23 0.0494740976152105 0.0 0.0 24-25 0.05547886935742602 0.0 3.7296718895748584E-5 26-27 0.061576882896880916 0.0 3.7296718895748584E-5 28-29 0.06912946847327 0.0 3.7296718895748584E-5 30-31 0.11921896195026035 0.0 3.7296718895748584E-5 32-33 0.12839395479861448 0.0 3.7296718895748584E-5 34-35 0.1367297714718143 0.0 3.7296718895748584E-5 36-37 0.14605395119575146 0.0 3.7296718895748584E-5 38-39 0.15563920795195885 0.0 3.7296718895748584E-5 40-41 0.16649255315062167 0.0 3.7296718895748584E-5 42-43 0.178017239289408 0.0 3.7296718895748584E-5 44-45 0.1901946180088699 0.0 3.7296718895748584E-5 46-47 0.2033976564979649 0.0 3.7296718895748584E-5 48-49 0.21880120140190906 0.0 3.7296718895748584E-5 50-51 0.23580850521837043 0.0 3.7296718895748584E-5 52-53 0.25404660075839147 0.0 3.7296718895748584E-5 54-55 0.27424277404043934 0.0 3.7296718895748584E-5 56-57 0.29744133319359495 0.0 3.7296718895748584E-5 58-59 0.3224301348537465 0.0 3.7296718895748584E-5 60-61 0.3487056733158014 0.0 3.7296718895748584E-5 62-63 0.3790465541374929 0.0 3.7296718895748584E-5 64-65 0.4107860619177749 0.0 3.7296718895748584E-5 66-67 0.44498715314517634 0.0 3.7296718895748584E-5 68-69 0.48334682852945376 0.0 3.7296718895748584E-5 70-71 0.5273010117480935 0.0 3.7296718895748584E-5 72-73 0.5743321742756324 0.0 3.7296718895748584E-5 74-75 0.6256338111167346 0.0 3.7296718895748584E-5 76-77 0.6857374736172335 0.0 3.7296718895748584E-5 78-79 0.7525918422378628 0.0 3.7296718895748584E-5 80-81 0.8259544883058003 0.0 3.7296718895748584E-5 82-83 0.907727544484729 0.0 3.7296718895748584E-5 84-85 1.000876099926861 0.0 3.7296718895748584E-5 86-87 1.102975867903973 0.0 3.7296718895748584E-5 88-89 1.2163392449876007 0.0 3.7296718895748584E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 29770 0.0 81.2643 3 CGGAAGA 29770 0.0 81.05687 4 GATCGGA 29965 0.0 80.925674 1 GAAGAGC 29780 0.0 80.77683 6 ATCGGAA 30195 0.0 80.19915 2 GGAAGAG 31485 0.0 76.67406 5 GAGCACA 38740 0.0 62.09432 9 AGAGCAC 38875 0.0 61.91534 8 AAGAGCA 38870 0.0 61.80112 7 ATCGGAC 145 0.0 45.86327 2 GCCGTCT 25525 0.0 45.669464 50-51 ATGCCGT 25540 0.0 45.62781 48-49 TCGTATG 25845 0.0 45.60392 44-45 TCTCGTA 25180 0.0 45.47848 42-43 TTGTGTT 24690 0.0 45.380558 1 CTGCTTG 25645 0.0 45.373257 58-59 GTATGCC 25975 0.0 45.256824 46-47 AATCTCG 25350 0.0 45.172653 40-41 TGCCGTC 25550 0.0 44.99265 50-51 CGTATGC 25700 0.0 44.98336 46-47 >>END_MODULE