FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_30423-1.3410000003574b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_30423-1.3410000003574b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681201
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2167808.08518272222038TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA292961.0926446767698506No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC272961.0180512389783534No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC125900.46956569089747463No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT107130.39955974953015455No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT93140.3473816397950023No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC55180.20580329486674068No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT54430.20300604094955957No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT50920.1899148926171518TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT36750.13706544194187603No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC33710.12572723939756847No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG31210.11640305967363133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG297700.081.26433
CGGAAGA297700.081.056874
GATCGGA299650.080.9256741
GAAGAGC297800.080.776836
ATCGGAA301950.080.199152
GGAAGAG314850.076.674065
GAGCACA387400.062.094329
AGAGCAC388750.061.915348
AAGAGCA388700.061.801127
ATCGGAC1450.045.863272
GCCGTCT255250.045.66946450-51
ATGCCGT255400.045.6278148-49
TCGTATG258450.045.6039244-45
TCTCGTA251800.045.4784842-43
TTGTGTT246900.045.3805581
CTGCTTG256450.045.37325758-59
GTATGCC259750.045.25682446-47
AATCTCG253500.045.17265340-41
TGCCGTC255500.044.9926550-51
CGTATGC257000.044.9833646-47
Produced by FastQC (version 0.11.2)