FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_30245-1.34100000035771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_30245-1.34100000035771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256156
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT318951.4136877059919615TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26170.11599375220507802No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG24470.10845881224525254No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC24390.1081042268353784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG53400.070.094353
CGGAAGA53650.069.679194
GATCGGA54950.068.2290951
CGCCGAG353.3847173E-667.9693451
ATCGGAA57500.065.096322
GAAGAGC61600.060.673056
ACGACGG250.004989169557.0942421
GGAAGAG70000.053.799345
CGGGGCG902.2191671E-1047.578541
CGAGGCG502.8276963E-547.500492
TTGTGTT246000.044.406631
TATGCCG41050.043.62117448-49
TCGTATG41700.043.56672344-45
ATGCCGT41050.043.56332448-49
CGTATGC41150.043.5137246-47
TGCCGTC41000.043.3910150-51
GCCGTCT41500.043.26886450-51
TCTCGTA41600.042.64389442-43
AATCTCG42000.042.57654640-41
CCGTCTT42150.042.3766752-53
Produced by FastQC (version 0.11.2)