##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l05n01_30245-1.34100000035771.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2256156 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69530209790458 33.0 33.0 33.0 33.0 33.0 2 32.74144208113269 33.0 33.0 33.0 33.0 33.0 3 32.77215626933599 33.0 33.0 33.0 33.0 33.0 4 36.7042318882205 37.0 37.0 37.0 37.0 37.0 5 36.711516845466356 37.0 37.0 37.0 37.0 37.0 6 36.610994541157616 37.0 37.0 37.0 37.0 37.0 7 36.57330477147857 37.0 37.0 37.0 37.0 37.0 8 36.521237893124415 37.0 37.0 37.0 37.0 37.0 9 36.53392717524852 37.0 37.0 37.0 37.0 37.0 10-11 36.49142169247162 37.0 37.0 37.0 37.0 37.0 12-13 36.466232831417685 37.0 37.0 37.0 37.0 37.0 14-15 38.41578884616135 40.0 37.0 40.0 37.0 40.0 16-17 38.4089615257101 40.0 37.0 40.0 37.0 40.0 18-19 38.43606869383146 40.0 37.0 40.0 37.0 40.0 20-21 38.3974044791229 40.0 37.0 40.0 37.0 40.0 22-23 38.29960073682848 40.0 37.0 40.0 37.0 40.0 24-25 38.289700933800674 40.0 37.0 40.0 37.0 40.0 26-27 38.219181430716674 40.0 37.0 40.0 37.0 40.0 28-29 38.12040989186918 40.0 37.0 40.0 37.0 40.0 30-31 38.07135366526073 40.0 37.0 40.0 37.0 40.0 32-33 37.974777231716246 40.0 37.0 40.0 35.0 40.0 34-35 37.92202733321632 40.0 37.0 40.0 33.0 40.0 36-37 37.85636166116173 40.0 37.0 40.0 33.0 40.0 38-39 37.7386100517872 40.0 37.0 40.0 33.0 40.0 40-41 37.649785963381966 40.0 37.0 40.0 33.0 40.0 42-43 37.51898450284466 40.0 37.0 40.0 33.0 40.0 44-45 37.433834584133365 40.0 37.0 40.0 33.0 40.0 46-47 37.31026843888455 40.0 37.0 40.0 33.0 40.0 48-49 37.225966865766374 40.0 37.0 40.0 33.0 40.0 50-51 37.10832318332598 40.0 37.0 40.0 33.0 40.0 52-53 36.99849722270977 37.0 37.0 40.0 33.0 40.0 54-55 36.84975484851225 37.0 37.0 40.0 33.0 40.0 56-57 36.66186558021697 37.0 37.0 40.0 33.0 40.0 58-59 36.44502463482135 37.0 37.0 40.0 33.0 40.0 60-61 36.23930171495233 37.0 37.0 40.0 33.0 40.0 62-63 36.020963975895285 37.0 37.0 40.0 33.0 40.0 64-65 35.79399075241251 37.0 37.0 40.0 33.0 40.0 66-67 35.48780314836385 37.0 33.0 40.0 27.0 40.0 68-69 35.23949740177541 37.0 33.0 37.0 27.0 40.0 70-71 34.95015858832457 37.0 33.0 37.0 27.0 40.0 72-73 34.60932954104237 37.0 33.0 37.0 27.0 40.0 74-75 34.17427341017199 37.0 33.0 37.0 27.0 38.5 76-77 31.437399718813772 33.0 30.0 35.0 24.5 37.0 78-79 33.78637780366252 37.0 33.0 37.0 27.0 37.0 80-81 34.09761270940484 37.0 33.0 37.0 27.0 37.0 82-83 33.95178103819062 37.0 33.0 37.0 27.0 37.0 84-85 33.8007493719406 37.0 33.0 37.0 27.0 37.0 86-87 33.69080994399323 37.0 33.0 37.0 27.0 37.0 88-89 33.41034972758976 37.0 33.0 37.0 27.0 37.0 90-91 33.3917594793977 37.0 33.0 37.0 27.0 37.0 92-93 33.24481086414237 37.0 33.0 37.0 27.0 37.0 94-95 33.06619156654061 37.0 33.0 37.0 27.0 37.0 96-97 32.960241224454336 37.0 33.0 37.0 27.0 37.0 98-99 32.84761160132544 33.0 33.0 37.0 27.0 37.0 100-101 31.28167755243875 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.11514771066382679 2306 2 0.06992264327565323 2306 3 0.08619661714371318 2306 4 0.1293872325300356 2306 5 0.1454979908909877 2306 6 0.16620250441632578 2306 7 0.09058780419213264 2306 8 0.18302102442225276 2306 9 0.1410429307424934 2306 10-11 0.21147193889274973 2306 12-13 0.17415597975409014 2306 14-15 0.33579752630635085 2306 16-17 0.42225385254533165 2306 18-19 0.4420125864194233 2306 20-21 0.4079449726713307 2306 22-23 0.4774463555948216 2306 24-25 0.38214916306300495 2306 26-27 0.4648437712703739 2306 28-29 0.44477057260097297 2306 30-31 0.45720797078596576 2306 32-33 0.5559252357313227 2306 34-35 0.4476687178354126 2306 36-37 0.41877915072590355 2306 38-39 0.47932136537943393 2306 40-41 0.6297629782044467 2306 42-43 0.5521284012124781 2306 44-45 0.45209186226471587 2306 46-47 0.4800776953148045 2306 48-49 0.46776040271627295 2306 50-51 0.39448458223346705 2306 52-53 0.24523496432710346 2306 54-55 0.2954288423725586 2306 56-57 0.17719930048059496 2306 58-59 0.4944217952645644 2306 60-61 0.369479105470532 2306 62-63 0.3811973720415196 2306 64-65 0.3273897419339846 2306 66-67 0.1769109613607398 2306 68-69 0.2934197850542617 2306 70-71 0.3650659244737753 2306 72-73 0.2642716701454617 2306 74-75 0.21032739049800142 2306 76-77 -0.1968030990610785 2306 78-79 0.14070868407786463 2306 80-81 0.5019141037684562 2306 82-83 0.4668523290651265 2306 84-85 0.33927079622801415 2306 86-87 0.3102200882534376 2306 88-89 0.6245156621445673 2306 90-91 0.44216398128377676 2306 92-93 0.476567874128655 2306 94-95 0.42859961388658263 2306 96-97 0.504454382767122 2306 98-99 0.4153541737534212 2306 100-101 0.4435155679390057 2307 1 0.1621884983026618 2307 2 0.16339428223820818 2307 3 0.04548386871380927 2307 4 0.13978378324605956 2307 5 0.12983668456198671 2307 6 0.21253043148988837 2307 7 0.2072141955718081 2307 8 0.18768345290592237 2307 9 0.13507772955161812 2307 10-11 0.19204588776382536 2307 12-13 0.20206186774207424 2307 14-15 0.45331118879971655 2307 16-17 0.40087364235559875 2307 18-19 0.3867684957531736 2307 20-21 0.42260146599005566 2307 22-23 0.458414780677586 2307 24-25 0.3835476513180254 2307 26-27 0.4442883567740026 2307 28-29 0.48652477827806706 2307 30-31 0.37943107852162683 2307 32-33 0.3509485879220833 2307 34-35 0.43429277039165726 2307 36-37 0.3880370388484451 2307 38-39 0.4978112864121087 2307 40-41 0.5143732797604841 2307 42-43 0.3950053222315333 2307 44-45 0.4177962606144021 2307 46-47 0.5259747021815002 2307 48-49 0.42854606316556954 2307 50-51 0.46979169297754453 2307 52-53 0.4060198638578214 2307 54-55 0.3961714588591789 2307 56-57 0.3925845135097603 2307 58-59 0.5385650850439916 2307 60-61 0.44700329266931504 2307 62-63 0.5448429249529951 2307 64-65 0.48280936450235146 2307 66-67 0.4513120183166919 2307 68-69 0.42294294541345323 2307 70-71 0.4093519866079802 2307 72-73 0.4390770952546532 2307 74-75 0.404897275262897 2307 76-77 0.08377341290416496 2307 78-79 0.38953286462862025 2307 80-81 0.5070116575353651 2307 82-83 0.5048489986446398 2307 84-85 0.6517770537821335 2307 86-87 0.5781731612031038 2307 88-89 0.8529149858080913 2307 90-91 0.6136952538360063 2307 92-93 0.5492780498527949 2307 94-95 0.6916655871294779 2307 96-97 0.6300277749661873 2307 98-99 0.5525754272506447 2307 100-101 0.49340399324603723 2201 1 0.1029043917751622 2201 2 0.10622120493207632 2201 3 0.11963362072066275 2201 4 0.1793041306356571 2201 5 0.12906780620810565 2201 6 0.1423115202694447 2201 7 0.1558196573967976 2201 8 0.1994723898220414 2201 9 0.13997265614963084 2201 10-11 0.18763001620236253 2201 12-13 0.19883237946447707 2201 14-15 0.31904530827465294 2201 16-17 0.3316475004915631 2201 18-19 0.38494330602347304 2201 20-21 0.311571846246224 2201 22-23 0.28420905592010115 2201 24-25 0.18876965000336554 2201 26-27 0.25529148407714786 2201 28-29 0.2961789822101011 2201 30-31 0.22551059568195342 2201 32-33 0.24185670098304968 2201 34-35 0.23813696296979003 2201 36-37 0.1877850618749619 2201 38-39 0.23258849096163914 2201 40-41 0.22369383829284573 2201 42-43 0.2291572653425078 2201 44-45 0.23129326278558437 2201 46-47 0.33011389705032457 2201 48-49 0.3539371752505005 2201 50-51 0.2104492234040194 2201 52-53 0.007775137447048053 2201 54-55 0.25323836721127435 2201 56-57 0.1274226705920114 2201 58-59 0.32621124164817417 2201 60-61 0.06981886146002836 2201 62-63 0.27272811109725126 2201 64-65 0.14593626926900072 2201 66-67 0.20448911928675528 2201 68-69 0.25042098457445405 2201 70-71 -0.012831349049243101 2201 72-73 0.13268106314502148 2201 74-75 0.09871776407830879 2201 76-77 -0.029810436666576123 2201 78-79 0.08985097310819867 2201 80-81 0.1993756596866305 2201 82-83 0.2884934023655319 2201 84-85 0.3810579732490922 2201 86-87 0.21604500153136286 2201 88-89 0.5948857843659496 2201 90-91 0.1774402491550049 2201 92-93 0.2959728688875103 2201 94-95 0.35089750554072907 2201 96-97 0.34958616789091224 2201 98-99 0.3709364813709115 2201 100-101 0.30886024088063735 1101 1 0.09869243340498457 1101 2 0.08640175630370095 1101 3 0.06489720823171297 1101 4 0.11152681713900137 1101 5 0.1028538460304631 1101 6 0.09286687607614397 1101 7 0.1852349547040717 1101 8 0.11863279679143801 1101 9 0.09618010559427148 1101 10-11 0.1021048332702037 1101 12-13 0.14950842676933007 1101 14-15 0.13477407308170797 1101 16-17 0.20650868583366133 1101 18-19 0.1906632278946745 1101 20-21 0.2594022173580015 1101 22-23 0.21271829241518958 1101 24-25 0.2991883775923512 1101 26-27 0.2654194637092431 1101 28-29 0.18874139293046 1101 30-31 0.2708902102854367 1101 32-33 0.258780331056812 1101 34-35 0.26531043027522117 1101 36-37 0.2618276440122358 1101 38-39 0.2266911273226242 1101 40-41 0.2142009952403825 1101 42-43 0.1274642804143653 1101 44-45 0.17348806002969752 1101 46-47 0.2330330376335752 1101 48-49 0.24653398596308307 1101 50-51 0.0726731731839223 1101 52-53 0.030174196791897145 1101 54-55 0.012316758379995463 1101 56-57 0.09720587290088645 1101 58-59 0.11863741285129947 1101 60-61 0.037831338745341725 1101 62-63 0.19553822119665654 1101 64-65 0.2254474784190066 1101 66-67 0.04306666989069896 1101 68-69 0.13526633037211866 1101 70-71 0.0535759896157586 1101 72-73 0.035102667117534736 1101 74-75 -0.0755199456984812 1101 76-77 -0.28165433455496114 1101 78-79 -0.05356084532981242 1101 80-81 0.16158425579768476 1101 82-83 0.35040658611396935 1101 84-85 0.13701276738360235 1101 86-87 0.11513330456225646 1101 88-89 0.2759275016446523 1101 90-91 0.16885132906210742 1101 92-93 0.13355845587381765 1101 94-95 0.21666484887014548 1101 96-97 0.22929250468043705 1101 98-99 0.08686523257345868 1101 100-101 0.24705783676818882 2304 1 0.12847990162427436 2304 2 0.12846062746545073 2304 3 0.06105380596221721 2304 4 0.09366861295944062 2304 5 0.09850729831576643 2304 6 0.07020646308806988 2304 7 0.08422944997843729 2304 8 0.1321262142339208 2304 9 0.06779106724673056 2304 10-11 0.13613460375726305 2304 12-13 0.14076045703981777 2304 14-15 0.28584097727495816 2304 16-17 0.19305724958926618 2304 18-19 0.2181876422412401 2304 20-21 0.17925806618795548 2304 22-23 0.18057814754074286 2304 24-25 0.14656675397344543 2304 26-27 0.16532496670532026 2304 28-29 0.25607027237838764 2304 30-31 0.3699024728833322 2304 32-33 0.20106703276546511 2304 34-35 0.2343251614304549 2304 36-37 0.18894608287769898 2304 38-39 0.1799520601228295 2304 40-41 0.21451090138803153 2304 42-43 0.312115042443871 2304 44-45 0.2711784652032918 2304 46-47 0.3286397917322432 2304 48-49 0.35757504105908566 2304 50-51 0.3000122098805136 2304 52-53 0.17292363507717567 2304 54-55 0.3582077110387232 2304 56-57 0.2223820131149452 2304 58-59 0.3482836858800269 2304 60-61 0.16408593977459418 2304 62-63 0.26203628172820004 2304 64-65 0.17542098466665834 2304 66-67 0.16211402688339405 2304 68-69 0.17067927913461745 2304 70-71 0.10618330503606899 2304 72-73 0.02311459795390647 2304 74-75 0.15122113408308735 2304 76-77 0.0995020823576418 2304 78-79 0.18040713260725738 2304 80-81 0.257048399609225 2304 82-83 0.40590871740662493 2304 84-85 0.3644010179940622 2304 86-87 0.28428781581148854 2304 88-89 0.4199873193487704 2304 90-91 0.29183517023167127 2304 92-93 0.3143717904859855 2304 94-95 0.30589250488846886 2304 96-97 0.28933222172401685 2304 98-99 0.22813872514183942 2304 100-101 0.4925494177297942 2202 1 0.0634782865224679 2202 2 0.0771328261350348 2202 3 -0.010693171250778732 2202 4 -0.010143429400457649 2202 5 -0.007349824819321782 2202 6 0.08153898239222457 2202 7 -0.03364778351971154 2202 8 0.05851429706116562 2202 9 0.06612744537088133 2202 10-11 0.13575019612433437 2202 12-13 0.09455500804821781 2202 14-15 -0.011396461455049689 2202 16-17 0.08125427324481649 2202 18-19 -0.02382631599128615 2202 20-21 0.11362421579865156 2202 22-23 0.1165734724576808 2202 24-25 0.07924770233978506 2202 26-27 -0.012611000204536538 2202 28-29 0.10133923038976178 2202 30-31 0.21470003562098583 2202 32-33 0.21174975841395138 2202 34-35 0.22119083187195088 2202 36-37 0.11673990742536944 2202 38-39 0.13371457716182533 2202 40-41 0.10999210120092329 2202 42-43 0.26888473160713744 2202 44-45 0.15461502655102066 2202 46-47 0.26323875726289003 2202 48-49 0.02099720722559084 2202 50-51 0.03760534630099954 2202 52-53 -0.007248678190919122 2202 54-55 -0.03644958580358093 2202 56-57 0.0170726842601141 2202 58-59 0.0693494386179978 2202 60-61 0.009661968178939162 2202 62-63 6.378850350756693E-4 2202 64-65 0.16537330900590774 2202 66-67 0.16946977684687425 2202 68-69 0.1039424683508301 2202 70-71 -0.04432796515772708 2202 72-73 0.1630739590437642 2202 74-75 0.2641700955257136 2202 76-77 -0.2230360266731246 2202 78-79 0.0692837053210269 2202 80-81 0.016981007643458668 2202 82-83 0.2405523160410965 2202 84-85 0.18642564528084193 2202 86-87 0.1449183354721768 2202 88-89 0.34857261079757507 2202 90-91 -0.021208016652359163 2202 92-93 0.19560457557825117 2202 94-95 0.243845922939812 2202 96-97 0.22171052279775694 2202 98-99 0.29329220002331624 2202 100-101 0.37091509554312196 1102 1 0.0488940110717877 1102 2 0.010277747956131122 1102 3 0.06057502172770057 1102 4 0.04263076590677173 1102 5 0.12766819567843868 1102 6 0.01886959557221246 1102 7 0.12828488048882036 1102 8 0.009609169941988682 1102 9 0.1189906012668942 1102 10-11 0.11611441799299627 1102 12-13 0.07455705031549797 1102 14-15 0.16979593088409928 1102 16-17 0.14538959664884032 1102 18-19 0.12514859257898792 1102 20-21 0.12957685815532471 1102 22-23 0.18110337911841157 1102 24-25 0.036749292032020264 1102 26-27 0.0668270988894264 1102 28-29 0.11083206655137445 1102 30-31 0.17326664139428516 1102 32-33 0.2186742162864448 1102 34-35 0.2105998585498483 1102 36-37 0.20838354278548366 1102 38-39 0.27726803135560374 1102 40-41 0.21339754601941507 1102 42-43 0.32174119154485936 1102 44-45 0.13619934767771014 1102 46-47 0.302244268753995 1102 48-49 0.08607965001639428 1102 50-51 0.17881150840010207 1102 52-53 0.15524226134051133 1102 54-55 0.06347647168552584 1102 56-57 0.005170259677527156 1102 58-59 0.2927788597500083 1102 60-61 0.2748745277879152 1102 62-63 0.24669007955169775 1102 64-65 0.17008606793920933 1102 66-67 0.21085486451729452 1102 68-69 0.12756497222312646 1102 70-71 -0.03003771401863986 1102 72-73 0.09113497452887742 1102 74-75 0.12798064103826334 1102 76-77 0.03885892474172081 1102 78-79 0.20752284047104297 1102 80-81 0.15920280313118695 1102 82-83 0.29160218251650605 1102 84-85 0.21730943764203658 1102 86-87 0.23191102994253043 1102 88-89 0.5359806459953589 1102 90-91 0.26839445147786734 1102 92-93 0.3280349669068059 1102 94-95 0.39479422607636394 1102 96-97 0.2936332456996311 1102 98-99 0.21852261870964185 1102 100-101 0.21870328899031577 2305 1 0.03849313379097907 2305 2 0.04979947440445898 2305 3 0.06464584621137703 2305 4 0.09540401101393314 2305 5 0.0634022504937164 2305 6 0.11416355045324167 2305 7 0.13331482362585234 2305 8 0.11868393971332836 2305 9 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-0.686342939795658 1213 96-97 -0.7703670217401495 1213 98-99 -1.0330240487540578 1213 100-101 -0.9338137401106188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 21.0 11 9.0 12 23.0 13 56.0 14 130.0 15 303.0 16 645.0 17 1103.0 18 1669.0 19 2495.0 20 3315.0 21 4349.0 22 5556.0 23 7299.0 24 9192.0 25 11751.0 26 15117.0 27 20092.0 28 27919.0 29 35506.0 30 35760.0 31 42196.0 32 55969.0 33 79795.0 34 121711.0 35 211854.0 36 433620.0 37 808474.0 38 319647.0 39 575.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.29946641353351 25.799425122518876 24.156220335957904 14.744888127989714 2 29.160971138520566 24.105336687711308 33.51957931986973 13.2141128538984 3 36.54694179673735 20.01493698321695 27.34274614441891 16.095375075626787 4 28.196878874972793 20.413351119693964 34.16767856517446 17.22209144015878 5 31.11225464905796 18.982995856669486 33.810605295023926 16.094144199248632 6 25.375328656351776 19.80576697710619 38.55983362852569 16.259070738016344 7 31.5002154106365 21.13009915980987 29.62928981861183 17.740395610941796 8 28.01885153331596 28.827306267829 26.16414822379304 16.98969397506201 9 29.91012146323215 25.643306579864156 26.29516753274153 18.15140442416216 10-11 23.98845903418099 29.28485725822501 30.02747616833552 16.699207539258477 12-13 23.234386274707955 27.672089164047165 29.821253494882445 19.272271066362432 14-15 22.222133575869755 28.368007354101398 30.600809518490742 18.809049551538102 16-17 22.002925369826585 28.409197185439638 31.389129591667132 18.198747853066653 18-19 20.67622983517097 28.454947264284918 32.32334111648308 18.54548178406103 20-21 21.48809745425405 28.847251697134418 31.943491496155406 17.721159352456127 22-23 20.55877133865199 30.60162538203969 30.947702199303105 17.89190108000522 24-25 20.74089734929677 28.750339072298193 31.683070674190972 18.82569290421407 26-27 20.700009219220657 28.867041995323017 30.78206914770078 19.650879637755544 28-29 21.215975313763767 29.726158120271823 30.685333815569493 18.372532750394917 30-31 20.0583869200534 29.3327899311927 31.33457970104904 19.27424344770486 32-33 19.905649254750116 30.304154499954787 30.87091495446237 18.919281290832725 34-35 20.173627178262496 29.315836316283097 31.7389843610105 18.771552144443913 36-37 20.904476418510605 29.33806236686296 30.97271704606198 18.784744168564455 38-39 21.502944745167348 29.96091771888903 30.293144810544568 18.242992725399052 40-41 21.32994212622569 29.996137020310677 29.972666592938353 18.70125426052529 42-43 20.219465124401836 29.952293273808152 31.25229380570513 18.575947796084883 44-45 19.92395838365912 29.21119898516621 31.542289395173068 19.322553236001603 46-47 20.97015408319127 29.140415078717087 30.513542672294193 19.37588816579745 48-49 20.286156985183055 30.396333807557696 30.674485347619612 18.643023859639634 50-51 21.207005645961114 29.491043477779073 30.375758381675837 18.926192494583976 52-53 20.133259608022172 29.328083913883223 29.812543423506483 20.726113054588126 54-55 21.044384177625368 29.41226405141376 30.431527367863914 19.111824403096954 56-57 20.24341295040063 29.477074664607304 30.448740398827155 19.830771986164912 58-59 20.217230209536112 29.404282758817896 30.87150327037888 19.506983761267108 60-61 20.660039833696754 29.618875835095764 30.828337867412216 18.892746463795266 62-63 19.864906552867684 29.614270732959184 30.459740565975014 20.06108214819812 64-65 20.62125716980973 29.46019905018723 30.371356136346407 19.54718764365663 66-67 20.0231270821191 30.58623118347389 29.809793494577043 19.58084823982997 68-69 19.879783799695524 31.068929806719552 29.637901729230045 19.41338466435488 70-71 19.565400533317252 31.78494119232853 29.384126761724954 19.26553151262926 72-73 19.62072908441253 31.358118236687076 29.5031521300099 19.51800054889049 74-75 19.899573060255054 30.77369001279334 29.715573757227148 19.611163169724456 76-77 20.00015962668639 30.482334203298773 29.88606201851492 19.631444151499913 78-79 19.926069131590474 30.53656955805504 29.94658996806392 19.590771342290566 80-81 19.569880127729586 30.97482356503142 29.755066749502795 19.700229557736197 82-83 19.460625650310874 30.659436003869455 29.754923525103244 20.125014820716423 84-85 19.507427703867062 30.33067550270678 30.12033933053698 20.041557462889187 86-87 19.727681555973664 30.42140953367251 30.267419673462587 19.583489236891243 88-89 19.924523534027582 30.879960277877526 29.58655299755727 19.608963190537622 90-91 20.1481883776864 30.466665927756782 29.45725591035545 19.927889784201366 92-93 19.718797003150097 30.766776577177396 29.681868490507135 19.832557929165365 94-95 19.3675316291822 31.093405843107906 29.63404761313618 19.90501491457372 96-97 19.58061661430526 31.38973202132763 29.33646437695247 19.69318698741464 98-99 19.979954001950063 30.79956085918008 29.03337585514738 20.18710928372248 100-101 20.002380576094982 30.747556307606782 28.696093749480493 20.55396936681774 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26.0 1 17.5 2 20.0 3 45.0 4 57.5 5 54.0 6 44.0 7 36.5 8 40.5 9 44.5 10 55.5 11 69.0 12 63.5 13 65.0 14 83.5 15 102.5 16 138.0 17 185.5 18 278.0 19 435.5 20 760.5 21 1234.5 22 1925.0 23 3111.0 24 4759.0 25 6987.5 26 10782.0 27 16001.5 28 21720.0 29 27685.0 30 33655.0 31 39312.0 32 46244.0 33 56158.5 34 68373.0 35 84114.5 36 104535.5 37 126066.5 38 146836.5 39 158372.5 40 160209.0 41 150471.0 42 133040.0 43 122366.5 44 110797.5 45 100064.5 46 95978.0 47 86844.5 48 69829.5 49 55910.5 50 45984.0 51 37115.5 52 29630.5 53 23766.0 54 19400.0 55 15254.5 56 11316.5 57 8082.0 58 5771.0 59 4104.5 60 2976.0 61 2106.0 62 1449.0 63 979.0 64 675.0 65 457.0 66 304.0 67 219.5 68 164.0 69 108.0 70 72.0 71 60.0 72 45.5 73 32.5 74 24.0 75 15.5 76 13.5 77 12.5 78 6.5 79 5.0 80 5.0 81 2.0 82 1.0 83 2.0 84 1.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17064422850192984 2 0.0 3 4.8755493857694236E-4 4 0.021939972235962408 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.421403484510823E-4 12-13 0.0 14-15 0.0 16-17 0.001374018463262292 18-19 0.0 20-21 0.0 22-23 4.2107017422554117E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.751098771538847E-4 38-39 8.199787603339484E-4 40-41 0.00556255861740057 42-43 0.003545854098741399 44-45 0.0010194330533881523 46-47 0.004520963975895284 48-49 0.008487888248862223 50-51 0.005252296383760697 52-53 0.0014848264038479608 54-55 0.011612672173378082 56-57 0.019590843895546228 58-59 0.023513444992278903 60-61 0.012277519816892093 62-63 6.205244672797449E-4 64-65 0.01520284944835375 66-67 0.03508179398942272 68-69 0.02083189283010572 70-71 0.03714282168431616 72-73 0.030893253835284438 74-75 0.03038353730859036 76-77 0.03962491955343513 78-79 0.017729270493706997 80-81 0.013141821753460311 82-83 0.001041594641505286 84-85 0.010792693413044133 86-87 0.021629710002322534 88-89 0.021541063649854 90-91 0.02575176539210941 92-93 0.04379129811945628 94-95 0.02854412549486826 96-97 0.030051113486833356 98-99 0.0373644375654875 100-101 0.017707108905589863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2256156.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.5740736500446 #Duplication Level Percentage of deduplicated Percentage of total 1 92.73485752671161 61.73737234909691 2 3.6664609554453684 4.8818248336566565 3 1.0286950989830597 2.054532698494144 4 0.6104944975421216 1.6257242256926467 5 0.37315903414313695 1.2421358511112355 6 0.23220125442691975 0.92751500483103 7 0.1842653299272386 0.8587105552008024 8 0.14162448749006645 0.7542815248650804 9 0.09656749306945774 0.5786002256225625 >10 0.8063058120554587 10.148904977129064 >50 0.05968685161619945 2.7020133059128626 >100 0.06115147863656903 8.60157845548453 >500 0.0036571532646453627 1.4873869896796554 >1k 8.060958666039528E-4 0.9782232709047564 >5k 0.0 0.0 >10k+ 6.693082158620641E-5 1.421195732318167 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 31895 1.4136877059919615 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2617 0.11599375220507802 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2447 0.10845881224525254 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2439 0.1081042268353784 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.762012910454774E-4 0.0 0.0 2 6.205244672797449E-4 0.0 0.0 3 7.978171722168148E-4 0.0 0.0 4 7.978171722168148E-4 0.0 0.0 5 9.751098771538847E-4 0.0 0.0 6 0.0010194330533881523 0.0 0.0 7 0.001196725758325222 0.0 0.0 8 0.0014183416394965597 0.0 0.0 9 0.001551311168199362 0.0 0.0 10-11 0.002437774692884712 0.0 0.0 12-13 0.0036123388630928 0.0 0.0 14-15 0.0045652871521295515 0.0 2.2161588117133745E-5 16-17 0.005850659262923309 0.0 6.648476435140123E-5 18-19 0.007446293607356938 0.0 8.864635246853498E-5 20-21 0.009751098771538846 0.0 8.864635246853498E-5 22-23 0.011856449642666553 0.0 1.3296952870280247E-4 24-25 0.014693132921659673 0.0 1.3296952870280247E-4 26-27 0.01741900826006712 0.0 1.3296952870280247E-4 28-29 0.020987023946925656 0.0 1.3296952870280247E-4 30-31 0.03195701006490686 0.0 1.3296952870280247E-4 32-33 0.03627851974774794 0.0 1.3296952870280247E-4 34-35 0.03964708114155227 0.0 1.3296952870280247E-4 36-37 0.04379129811945628 0.0 1.7729270493706996E-4 38-39 0.04864468591710856 0.0 1.7729270493706996E-4 40-41 0.05363104324346366 0.0 1.7729270493706996E-4 42-43 0.059415217742035564 0.0 1.7729270493706996E-4 44-45 0.06471183730203053 0.0 1.7729270493706996E-4 46-47 0.07089492038671086 0.0 1.7729270493706996E-4 48-49 0.07796446699607651 0.0 1.7729270493706996E-4 50-51 0.08498969042920791 0.0 1.7729270493706996E-4 52-53 0.09212572180292497 0.0 1.7729270493706996E-4 54-55 0.10074657958049 0.0 1.7729270493706996E-4 56-57 0.1098106691203977 0.0 1.7729270493706996E-4 58-59 0.11863098119101693 0.0 1.7729270493706996E-4 60-61 0.128913958077367 0.0 1.7729270493706996E-4 62-63 0.14116931630614193 0.0 1.7729270493706996E-4 64-65 0.15271550371516862 0.0 1.7729270493706996E-4 66-67 0.16512599306076353 0.0 1.7729270493706996E-4 68-69 0.17884401610526932 0.0 1.7729270493706996E-4 70-71 0.19449009731596573 0.0 1.7729270493706996E-4 72-73 0.21175397445921293 0.0 1.7729270493706996E-4 74-75 0.23294045269919278 0.0 1.7729270493706996E-4 76-77 0.2569857758062829 0.0 1.7729270493706996E-4 78-79 0.28464343777646584 0.0 2.2161588117133743E-4 80-81 0.3135864718574425 0.0 2.2161588117133743E-4 82-83 0.34623049115398047 0.0 2.2161588117133743E-4 84-85 0.3810020229097633 0.0 2.6593905740560493E-4 86-87 0.4219787993383436 0.0 2.6593905740560493E-4 88-89 0.4685402959724416 0.0 2.6593905740560493E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 5340 0.0 70.09435 3 CGGAAGA 5365 0.0 69.67919 4 GATCGGA 5495 0.0 68.229095 1 CGCCGAG 35 3.3847173E-6 67.969345 1 ATCGGAA 5750 0.0 65.09632 2 GAAGAGC 6160 0.0 60.67305 6 ACGACGG 25 0.0049891695 57.094242 1 GGAAGAG 7000 0.0 53.79934 5 CGGGGCG 90 2.2191671E-10 47.57854 1 CGAGGCG 50 2.8276963E-5 47.50049 2 TTGTGTT 24600 0.0 44.40663 1 TATGCCG 4105 0.0 43.621174 48-49 TCGTATG 4170 0.0 43.566723 44-45 ATGCCGT 4105 0.0 43.563324 48-49 CGTATGC 4115 0.0 43.51372 46-47 TGCCGTC 4100 0.0 43.39101 50-51 GCCGTCT 4150 0.0 43.268864 50-51 TCTCGTA 4160 0.0 42.643894 42-43 AATCTCG 4200 0.0 42.576546 40-41 CCGTCTT 4215 0.0 42.37667 52-53 >>END_MODULE