FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l05n01_30230-1.34100000035714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l05n01_30230-1.34100000035714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244879
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT562522.505792071643951TruSeq Adapter, Index 27 (97% over 39bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA311461.3874244447028103No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC248271.1059393401604274No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC109630.48835594256973314No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT108310.48247589291004106No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT95990.4275954294195812No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC56770.25288668119751667No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT51210.22811919929760135No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT46290.2062026505660216No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC27480.1224119429154088No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.12187739294634589No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC26700.11893736811649983No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT25570.11390368924115732No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC25480.11350277676436013No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA23620.10521725224388485No Hit
CTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAA23270.10365814816745134No Hit
GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC22840.10174267744497588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA106700.057.0643234
TCGGAAG108300.056.4405363
GAAGAGC109850.055.46386
GATCGGA111150.054.9055941
ATCGGAA111350.054.894572
GGAAGAG127400.047.7861025
TTGTGTT228900.043.7219351
GCCGTCT70200.043.34115650-51
ATGCCGT70650.043.1606648-49
TGTGTTT430400.043.1235431
TCTCGTA68850.042.97783342-43
TATGCCG70500.042.94932648-49
TCGTATG71300.042.93277744-45
CGTATGC70600.042.8534246-47
TGCCGTC70300.042.73893450-51
GTATGCC71950.042.67646846-47
CTCGTAT68950.042.50171744-45
TCGATCT69150.042.4098338-39
ATCTCGT69300.042.0476542-43
ACTCGAT71650.041.99060436-37
Produced by FastQC (version 0.11.2)