##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_53115004-1.3420000003588a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4698037 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.405294168607018 33.0 33.0 33.0 22.0 33.0 2 29.72789656616157 33.0 33.0 33.0 15.0 33.0 3 29.428302288807007 33.0 33.0 33.0 15.0 33.0 4 32.63149566510438 37.0 37.0 37.0 15.0 37.0 5 32.76759463580215 37.0 37.0 37.0 15.0 37.0 6 33.62551550785999 37.0 37.0 37.0 22.0 37.0 7 32.670102002176655 37.0 37.0 37.0 15.0 37.0 8 33.293544516571494 37.0 37.0 37.0 22.0 37.0 9 32.31941681174499 37.0 37.0 37.0 15.0 37.0 10-11 32.39756179016896 37.0 33.0 37.0 15.0 37.0 12-13 32.4049783984247 37.0 33.0 37.0 10.5 37.0 14-15 33.792923086812635 37.0 33.0 40.0 15.0 40.0 16-17 33.673911891285655 37.0 33.0 40.0 10.5 40.0 18-19 33.739681807529394 37.0 33.0 40.0 10.5 40.0 20-21 33.80801705052557 37.0 33.0 40.0 15.0 40.0 22-23 34.92573366280428 37.0 33.0 40.0 22.0 40.0 24-25 34.04523719161854 37.0 33.0 40.0 15.0 40.0 26-27 33.919049488115995 37.0 33.0 40.0 14.0 40.0 28-29 33.411511126881294 37.0 33.0 40.0 15.0 40.0 30-31 33.45252485240112 37.0 33.0 40.0 10.5 40.0 32-33 33.1487328643857 37.0 33.0 40.0 6.0 40.0 34-35 33.36756021717156 37.0 33.0 40.0 10.5 40.0 36-37 33.14607771288306 37.0 33.0 40.0 6.0 40.0 38-39 33.446942946596636 37.0 33.0 40.0 15.0 40.0 40-41 32.92512415291748 37.0 33.0 40.0 6.0 40.0 42-43 32.735932049917864 37.0 33.0 40.0 6.0 40.0 44-45 32.622651545741334 37.0 33.0 40.0 6.0 40.0 46-47 32.606680832867 37.0 33.0 40.0 6.0 40.0 48-49 32.53712092944351 37.0 33.0 40.0 6.0 40.0 50-51 31.680370971961267 37.0 30.0 38.5 10.5 38.5 52-53 32.135379414849226 37.0 33.0 38.5 10.5 40.0 54-55 32.68246855016255 37.0 33.0 40.0 10.5 40.0 56-57 33.03747852560548 37.0 33.0 40.0 10.5 40.0 58-59 32.3539283534804 37.0 33.0 40.0 6.0 40.0 60-61 32.24052684131692 37.0 33.0 40.0 6.0 40.0 62-63 31.79884992391503 37.0 33.0 40.0 6.0 40.0 64-65 31.62948812450817 37.0 33.0 37.0 6.0 40.0 66-67 31.59436483790996 37.0 33.0 37.0 6.0 40.0 68-69 31.839076725023666 37.0 33.0 37.0 15.0 40.0 70-71 31.36440528246159 37.0 33.0 37.0 10.5 40.0 72-73 31.932809171149565 37.0 33.0 37.0 15.0 37.0 74-75 32.11863731596835 37.0 30.0 37.0 22.0 37.0 76-77 31.719090122108447 37.0 27.0 37.0 22.0 37.0 78-79 31.055546710253665 33.0 27.0 37.0 15.0 37.0 80-81 30.43403106446373 33.0 27.0 37.0 15.0 37.0 82-83 29.99094249364149 33.0 27.0 37.0 6.0 37.0 84-85 29.798686451383844 33.0 27.0 37.0 6.0 37.0 86-87 29.616326776481326 33.0 27.0 37.0 6.0 37.0 88-89 29.416224584863848 33.0 27.0 37.0 6.0 37.0 90-91 29.343234631826014 33.0 27.0 37.0 6.0 37.0 92-93 29.178451021139253 33.0 27.0 37.0 6.0 37.0 94-95 29.0446825982852 33.0 27.0 37.0 6.0 37.0 96-97 28.899047729934864 33.0 27.0 37.0 6.0 37.0 98-99 28.769672205646742 33.0 27.0 37.0 6.0 37.0 100-101 27.67198864121334 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.09387756128944247 2306 2 0.06913858529003036 2306 3 0.13454178816814633 2306 4 0.25019274329545027 2306 5 0.24535613290130698 2306 6 -0.09726109049486098 2306 7 0.7504750275464289 2306 8 -0.05754441061346682 2306 9 0.3741147027902727 2306 10-11 0.25275806189223715 2306 12-13 0.2387800767574788 2306 14-15 0.7222554975917532 2306 16-17 0.5712871509845812 2306 18-19 0.5179259486060559 2306 20-21 0.509332168312632 2306 22-23 0.3129326739441538 2306 24-25 0.6698861026652239 2306 26-27 0.24622135586105287 2306 28-29 0.4262853488692997 2306 30-31 0.5537652827805601 2306 32-33 0.5487765955916686 2306 34-35 0.3134351323206843 2306 36-37 0.45569908338531917 2306 38-39 0.3016593783004353 2306 40-41 0.5292505048004372 2306 42-43 0.4407517160805767 2306 44-45 0.6309798892311704 2306 46-47 0.5978354741278409 2306 48-49 0.6044294233566703 2306 50-51 0.5262650232191746 2306 52-53 0.40718846285276555 2306 54-55 0.5328093809871959 2306 56-57 0.4681339524170838 2306 58-59 0.7953737766869224 2306 60-61 0.5204192098694946 2306 62-63 0.6819777852850599 2306 64-65 0.5840139413500616 2306 66-67 0.4806984671794545 2306 68-69 0.38912427753899337 2306 70-71 0.5986469876312412 2306 72-73 0.03370325447099631 2306 74-75 0.20096433334492048 2306 76-77 0.32127351501395296 2306 78-79 0.4020801732591366 2306 80-81 0.6052448431886184 2306 82-83 0.6362181817095056 2306 84-85 0.6676009495391746 2306 86-87 0.5696918056224014 2306 88-89 0.5740873568207618 2306 90-91 0.3941978517007456 2306 92-93 0.4621500600086641 2306 94-95 0.3752810224492258 2306 96-97 0.2992784378747295 2306 98-99 0.3851041906810444 2306 100-101 0.2639723885207452 2307 1 0.0925629326227444 2307 2 0.052325599558706415 2307 3 0.09774966154810727 2307 4 0.3053763072129243 2307 5 0.17674900192627518 2307 6 -0.008859952589226339 2307 7 0.6942657037479805 2307 8 -0.026845910749912605 2307 9 0.3083310282223195 2307 10-11 0.27470082830383546 2307 12-13 0.21428641486198785 2307 14-15 0.5003888501144687 2307 16-17 0.5540019381506127 2307 18-19 0.4948201839401989 2307 20-21 0.32461455133526584 2307 22-23 0.15948704220839716 2307 24-25 0.517951635039843 2307 26-27 0.14944820991081542 2307 28-29 0.34004871757839084 2307 30-31 0.436035972738658 2307 32-33 0.4634195141476738 2307 34-35 0.29348155131966536 2307 36-37 0.3291872183639555 2307 38-39 0.150187839025115 2307 40-41 0.4159735683235297 2307 42-43 0.36849891337113405 2307 44-45 0.3629060113429503 2307 46-47 0.2996298062811533 2307 48-49 0.3330221529470805 2307 50-51 0.2765558078282879 2307 52-53 0.27617030084699934 2307 54-55 0.35472859678927904 2307 56-57 0.3339823911612001 2307 58-59 0.5218889593536744 2307 60-61 0.3465276772481616 2307 62-63 0.44396640062136683 2307 64-65 0.479898098518607 2307 66-67 0.36850186818653086 2307 68-69 0.15140680822020514 2307 70-71 0.2941834929450806 2307 72-73 -0.098091563647273 2307 74-75 0.10597650115983726 2307 76-77 0.22863485612770873 2307 78-79 0.4901471168012179 2307 80-81 0.41579229205949986 2307 82-83 0.4309416302663749 2307 84-85 0.4982914590173593 2307 86-87 0.5667505027129671 2307 88-89 0.6309771250154768 2307 90-91 0.6178171504906906 2307 92-93 0.5879348934161364 2307 94-95 0.46265780229588316 2307 96-97 0.3500801398735405 2307 98-99 0.37819119896861864 2307 100-101 0.39999622323285067 2201 1 0.17457406754142468 2201 2 -0.05962687506281128 2201 3 0.046954937462260204 2201 4 0.22326945414467758 2201 5 0.18594233502543744 2201 6 0.044239910419662465 2201 7 0.5787119435175541 2201 8 -0.045217648429492385 2201 9 0.2918683846450918 2201 10-11 0.18325443046759204 2201 12-13 0.18245617846450557 2201 14-15 0.458538943198171 2201 16-17 0.39197306463908177 2201 18-19 0.4095080900837189 2201 20-21 0.32625581024846184 2201 22-23 0.08234304347293175 2201 24-25 0.3887720888668085 2201 26-27 0.24637952116165707 2201 28-29 0.30576669151495395 2201 30-31 0.3955305106570961 2201 32-33 0.518229570700413 2201 34-35 0.2610519788021932 2201 36-37 0.3009411436862379 2201 38-39 0.18671002104766643 2201 40-41 0.4709491558046537 2201 42-43 0.27171557008545477 2201 44-45 0.46228164800036353 2201 46-47 0.25790020566294203 2201 48-49 0.3428639984632511 2201 50-51 0.2993477042770536 2201 52-53 0.2329228211322274 2201 54-55 0.4457613868416175 2201 56-57 0.39986633510021363 2201 58-59 0.43517358473567214 2201 60-61 0.34985410248263094 2201 62-63 0.532397237672189 2201 64-65 0.546548665802316 2201 66-67 0.40790086358503075 2201 68-69 0.2990099028143298 2201 70-71 0.3561640683077556 2201 72-73 -0.042348474673300274 2201 74-75 0.15576750189747912 2201 76-77 0.33115601949839046 2201 78-79 0.3470686806074639 2201 80-81 0.5679635133879586 2201 82-83 0.4535583361569948 2201 84-85 0.38469465196407526 2201 86-87 0.5036511665929773 2201 88-89 0.5419179729553427 2201 90-91 0.456811319586901 2201 92-93 0.3880025108245455 2201 94-95 0.4535874099392849 2201 96-97 0.5323656960925902 2201 98-99 0.5345169392475171 2201 100-101 0.43284774594176056 1101 1 0.035373609606885736 1101 2 -0.16281365964115224 1101 3 -0.04733834438577844 1101 4 0.02649973603637079 1101 5 -0.0033990445023661664 1101 6 -0.07271336958499575 1101 7 0.45685349250700824 1101 8 -0.01853730193862191 1101 9 0.19690178379034506 1101 10-11 -0.008138599290063553 1101 12-13 0.0406419200775332 1101 14-15 0.3063185589665167 1101 16-17 0.19042082501253788 1101 18-19 0.18192133396458843 1101 20-21 0.0996940707440146 1101 22-23 0.055335577781640666 1101 24-25 0.3297033841327206 1101 26-27 0.1335672985782992 1101 28-29 0.13421757269937729 1101 30-31 0.31531281388026144 1101 32-33 0.32831027519519296 1101 34-35 0.07376249205432117 1101 36-37 0.16016378686758515 1101 38-39 0.17603498802042594 1101 40-41 0.31224985483397205 1101 42-43 0.16089662401403615 1101 44-45 0.29979783821783457 1101 46-47 0.15838061651042068 1101 48-49 0.2800741713367785 1101 50-51 0.27385423615056226 1101 52-53 0.30252054374872017 1101 54-55 0.29214690563197365 1101 56-57 0.44636536732542 1101 58-59 0.41031721480475625 1101 60-61 0.3501230905152042 1101 62-63 0.4395088867054646 1101 64-65 0.39195848649312737 1101 66-67 0.4282523509829339 1101 68-69 0.2719626589108195 1101 70-71 0.3361532020644198 1101 72-73 0.13191866027670685 1101 74-75 0.4298633964949161 1101 76-77 0.5433558031437293 1101 78-79 0.4016353325594295 1101 80-81 0.5094314911186295 1101 82-83 0.4545122855998578 1101 84-85 0.53823701495158 1101 86-87 0.46194743018993023 1101 88-89 0.49821796858944367 1101 90-91 0.5455903063420493 1101 92-93 0.5566545307007154 1101 94-95 0.45655969940177954 1101 96-97 0.4087663823475971 1101 98-99 0.483158739680551 1101 100-101 0.4184570883238301 2304 1 0.05288304403204336 2304 2 -0.17270326788579382 2304 3 -0.10691782355819512 2304 4 0.03997473250969108 2304 5 -0.05967965107435447 2304 6 -0.05631232130579633 2304 7 0.4645442904486501 2304 8 0.048272096825414224 2304 9 0.16837420249920143 2304 10-11 0.10616229819603973 2304 12-13 0.0767715846089061 2304 14-15 0.2428308138620352 2304 16-17 0.18028653943093076 2304 18-19 0.27822869951909723 2304 20-21 0.08518209798315013 2304 22-23 0.1346206730984676 2304 24-25 0.37935229296379447 2304 26-27 0.32851796444236925 2304 28-29 0.2176236301829917 2304 30-31 0.45138298479953676 2304 32-33 0.3445490084281104 2304 34-35 0.2817706476734898 2304 36-37 0.28155791759056115 2304 38-39 0.1485113066713737 2304 40-41 0.30056755247498756 2304 42-43 0.25397652682654126 2304 44-45 0.3402896374746476 2304 46-47 0.266171169205208 2304 48-49 0.3705421381009657 2304 50-51 0.31031334984469794 2304 52-53 0.3468415323765939 2304 54-55 0.45694004923412734 2304 56-57 0.5034025679187835 2304 58-59 0.5211748866330908 2304 60-61 0.38204351627567945 2304 62-63 0.4123466356213932 2304 64-65 0.36528339959653877 2304 66-67 0.41085485041559977 2304 68-69 0.33873788608617517 2304 70-71 0.4050974489269876 2304 72-73 0.2888394146101483 2304 74-75 0.38709655800649756 2304 76-77 0.5047743271238652 2304 78-79 0.5367055464122394 2304 80-81 0.624439915729532 2304 82-83 0.5919234723265632 2304 84-85 0.4776262547563981 2304 86-87 0.48374057992544905 2304 88-89 0.4720153696210758 2304 90-91 0.5488729693427175 2304 92-93 0.44557255305945276 2304 94-95 0.4677938282321712 2304 96-97 0.4207380479672125 2304 98-99 0.39372402537693674 2304 100-101 0.5234089896222649 2202 1 -0.022792381764578096 2202 2 -0.21898636586022135 2202 3 -0.10169008215954634 2202 4 -0.09888501287659324 2202 5 -0.04750450063357903 2202 6 -0.07075945901186032 2202 7 0.2234511478143162 2202 8 -0.07308506584356422 2202 9 0.04209279283803369 2202 10-11 -0.06812428895911182 2202 12-13 -0.020037905319497895 2202 14-15 0.06625153750525214 2202 16-17 -0.030599061266904926 2202 18-19 -0.006899672585142014 2202 20-21 -0.017499707225695715 2202 22-23 -0.10938501088948982 2202 24-25 0.04270906169301725 2202 26-27 0.1659052594831536 2202 28-29 0.004660600223473921 2202 30-31 0.07353405667746671 2202 32-33 0.11701442815484597 2202 34-35 0.07817413846212418 2202 36-37 0.0067045395571270205 2202 38-39 -0.059364875650381066 2202 40-41 -0.01675735537124723 2202 42-43 0.07694261182908946 2202 44-45 -0.019909985468721914 2202 46-47 0.04168198445951532 2202 48-49 -0.056262849658764935 2202 50-51 -0.017586026685336265 2202 52-53 0.045331295972538044 2202 54-55 0.28536219990402856 2202 56-57 0.425467415226926 2202 58-59 0.21867457509909372 2202 60-61 0.13397824286942495 2202 62-63 0.14918000312296797 2202 64-65 0.16972687929054686 2202 66-67 0.1765738507733623 2202 68-69 0.1461441545521005 2202 70-71 0.09165505859699863 2202 72-73 0.1864128662862008 2202 74-75 0.3924736347322906 2202 76-77 0.3186996956384256 2202 78-79 0.37106774588242786 2202 80-81 0.1936499057202461 2202 82-83 0.2416539451748676 2202 84-85 0.2573624759093569 2202 86-87 0.2984582497315742 2202 88-89 0.3750472988405491 2202 90-91 0.3000690588876367 2202 92-93 0.17846571900858166 2202 94-95 0.28058983577330565 2202 96-97 0.13230560662604773 2202 98-99 0.1956798558863646 2202 100-101 0.3229322421175489 1102 1 -0.1935522146510671 1102 2 -0.33103815326207453 1102 3 -0.3046083058864859 1102 4 -0.34007263104564345 1102 5 -0.3798904774779288 1102 6 -0.03320275821981511 1102 7 -0.19857121122671373 1102 8 -0.23235055299598173 1102 9 -0.27737564291213346 1102 10-11 -0.20688405170352553 1102 12-13 -0.2606437512862385 1102 14-15 -0.24561067839583473 1102 16-17 -0.28107170616822685 1102 18-19 -0.19831860305617255 1102 20-21 -0.24893895384963827 1102 22-23 -0.14275538800963972 1102 24-25 -0.18439947503669885 1102 26-27 -0.1210024704873689 1102 28-29 -0.12204803270109466 1102 30-31 -0.09658829741604791 1102 32-33 -0.21809575440870788 1102 34-35 -0.1336814076785231 1102 36-37 -0.19711519779836095 1102 38-39 -0.14180662171224867 1102 40-41 -0.26982569567807957 1102 42-43 -0.30363404375888337 1102 44-45 -0.2235139732965692 1102 46-47 -0.2519072797938833 1102 48-49 -0.1722060821691187 1102 50-51 -0.27382698101845904 1102 52-53 -0.18574715731940472 1102 54-55 -0.023946687661343447 1102 56-57 0.0213172585658441 1102 58-59 -0.14634938906831252 1102 60-61 -0.14207487472690872 1102 62-63 -0.11283420816376477 1102 64-65 -0.0016072732020937508 1102 66-67 0.05051794628182549 1102 68-69 0.014588985613020355 1102 70-71 0.10470164233318258 1102 72-73 0.19757488259548595 1102 74-75 0.37582606925747797 1102 76-77 0.12356730559083573 1102 78-79 0.05562725047892769 1102 80-81 0.052888338069777774 1102 82-83 0.10660660866384575 1102 84-85 0.06680369402680242 1102 86-87 0.09227121712364905 1102 88-89 0.24419092038894874 1102 90-91 0.08724245199204717 1102 92-93 0.09171696731844392 1102 94-95 0.046292142758890265 1102 96-97 0.13559558338522137 1102 98-99 0.15377481573941765 1102 100-101 -0.10864656846062815 2305 1 0.21058385351561526 2305 2 -0.04279324792424788 2305 3 0.0027872537774911166 2305 4 -0.051424734161876984 2305 5 0.0012750129471044147 2305 6 0.24748052560261158 2305 7 -0.002083647143962253 2305 8 0.2551921251144762 2305 9 0.05398107025089871 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-1.1115686832054017 1213 96-97 -1.2339173411957916 1213 98-99 -1.2682325995104158 1213 100-101 -1.1958549114668244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3454.0 3 651.0 4 801.0 5 579.0 6 975.0 7 6923.0 8 40193.0 9 127805.0 10 167128.0 11 116637.0 12 68698.0 13 44357.0 14 30913.0 15 23821.0 16 20253.0 17 18981.0 18 18071.0 19 18125.0 20 19012.0 21 20225.0 22 21773.0 23 22469.0 24 24633.0 25 27578.0 26 32506.0 27 39269.0 28 48447.0 29 57616.0 30 68714.0 31 89074.0 32 120510.0 33 172415.0 34 259493.0 35 440031.0 36 858298.0 37 1346415.0 38 321085.0 39 109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.69969811807156 22.35635640078891 22.534847878909193 13.409097602230332 2 30.0036167908716 28.421169429048486 29.810486559727728 11.764727220352183 3 34.98644131498661 20.795330513877683 29.552088792589124 14.666139378546584 4 26.38343175374335 17.64541007650939 34.1737890279374 21.79736914180986 5 37.01538529807575 16.126572643030194 29.310277146999614 17.547764911894443 6 33.405899677953066 16.365232585304035 35.032946073406215 15.195921663336678 7 36.17128787990285 20.021218909913696 27.360790363867615 16.446702846315837 8 25.883533048693973 33.90124700704233 24.09274937474752 16.122470569516175 9 34.42295213202095 26.98298729234182 22.068752416365754 16.525308159271475 10-11 27.65483954228134 31.380037768303303 25.64704046121126 15.318082228204094 12-13 30.26560374691476 23.802858280861848 25.74421356219771 20.187324410025685 14-15 25.76936923410289 24.134582244918505 28.457384187259105 21.638664333719504 16-17 28.513069490814736 25.105210264836398 29.15274491512157 17.228975329227296 18-19 28.55807990091 25.452709657489685 29.422952800560363 16.566257641039947 20-21 26.526627917404337 28.922227182967585 27.79176016048503 16.759384739143048 22-23 31.08941382140687 25.202961273198238 26.93013612476149 16.7774887806334 24-25 24.695432096257335 31.464539162549197 26.785551990231415 17.05447675096205 26-27 22.410010830316814 33.749105882229834 26.594615465026127 17.24626782242722 28-29 24.31892496435615 31.440683935081232 26.37672776634708 17.863663334215538 30-31 28.516211931456475 27.03710363971375 26.725239471702828 17.72144495712695 32-33 23.47634797773312 26.503307585778945 29.24100357914744 20.779340857340497 34-35 19.04535773195326 26.390438417150207 36.12623510006245 18.437968750834077 36-37 21.71388773505515 27.06140270811629 29.229065764039525 21.99564379278904 38-39 19.78068609327643 26.383498857023753 26.33362178249623 27.502193267203584 40-41 22.64010248022914 25.870576850667916 28.616286249252234 22.87303441985072 42-43 25.46333986710335 26.061634364048107 30.771920292777278 17.703105476071265 44-45 21.84281450969931 29.222233351992905 31.413978275304633 17.520973863003153 46-47 24.427101347628625 30.568253167293648 27.561858441847264 17.442787043230464 48-49 19.894462985689614 28.98492840962535 31.01757262944853 20.103035975236512 50-51 19.00929914946947 26.63462521358862 32.10354069236413 22.25253494457778 52-53 27.142191913959664 26.20565948061796 26.795477969251476 19.856670636170907 54-55 29.24477461846645 26.264303203625044 26.710653169888793 17.780269008019715 56-57 29.45837090626006 26.141335731164343 26.322476226244675 18.07781713633092 58-59 26.48382392285424 26.127882405902774 26.57778898019931 20.810504691043683 60-61 20.9857422753531 26.484021677472697 25.90032842487915 26.62990762229505 62-63 24.884055706205572 26.707557791751153 26.933607534863345 21.47477896717993 64-65 20.72532842345639 29.02776152823336 30.804104791485372 19.44280525682488 66-67 19.494940878790445 28.630162476701308 28.421759372294673 23.45313727221357 68-69 19.183199170391614 26.511285563090926 35.655715082945356 18.6498001835721 70-71 18.663259072676304 33.21212913034944 30.406350566532247 17.71826123044201 72-73 18.380671595324177 38.37691836608993 25.70041865158515 17.541991387000742 74-75 19.049661962193777 38.06862378289577 25.14952175135981 17.732192503550635 76-77 19.355517768901745 37.07692703483497 25.56989107046383 17.997664125799453 78-79 19.062285129960447 36.30717471244711 26.45727856529877 18.173261592293677 80-81 18.359896377914183 36.29068551136846 26.35216027577321 18.99725783494415 82-83 18.04787112315632 35.25535298708902 26.716427011332662 19.980348878422006 84-85 18.35037772592198 34.272753065475946 27.11858235131997 20.2582868572821 86-87 18.559207319912126 33.734672406936674 27.929985250655566 19.776135022495634 88-89 19.069306841884845 33.838893045055094 27.50774324730959 19.58405686575047 90-91 19.500882005926158 32.95009524639159 27.15671906606013 20.392303681622124 92-93 19.16564510163794 33.48534149802616 26.847892391710992 20.50112100862491 94-95 18.770922288642186 33.87541631084543 26.756639624252777 20.59702177625961 96-97 19.381635289187766 34.051504135858615 26.51877450759598 20.048086067357637 98-99 20.022900896190254 32.82503777228041 26.299787591305474 20.852273740223858 100-101 20.167823700225888 32.93890083324569 25.717014666554906 21.17626079997352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2481.0 1 1571.0 2 546.0 3 511.5 4 524.0 5 449.0 6 524.5 7 706.5 8 895.0 9 1131.5 10 1380.0 11 1562.0 12 1647.5 13 1625.0 14 1530.0 15 1466.0 16 1393.5 17 1312.5 18 1316.0 19 1422.0 20 1660.0 21 2016.0 22 2560.0 23 3228.0 24 4157.0 25 5588.5 26 7583.5 27 10089.5 28 12901.0 29 16375.5 30 20597.5 31 25783.5 32 33600.5 33 48598.0 34 73152.0 35 114467.5 36 167524.5 37 227855.0 38 294906.5 39 331553.5 40 329923.5 41 298163.0 42 266661.5 43 270931.0 44 267838.5 45 251966.5 46 252595.5 47 234756.0 48 189676.5 49 152302.0 50 128467.0 51 113417.5 52 100083.5 53 85777.0 54 72500.0 55 58651.5 56 46345.0 57 36626.0 58 28734.5 59 21972.0 60 16545.5 61 12540.5 62 9317.0 63 6790.0 64 4927.0 65 3549.5 66 2569.0 67 1827.5 68 1359.5 69 1012.5 70 708.5 71 534.0 72 396.0 73 295.5 74 238.0 75 180.5 76 142.5 77 111.5 78 99.5 79 85.5 80 74.5 81 70.0 82 50.5 83 40.0 84 41.5 85 37.0 86 28.5 87 27.0 88 25.0 89 20.0 90 14.5 91 10.0 92 8.0 93 7.0 94 4.5 95 4.0 96 3.5 97 1.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2575330930769596 2 0.24610278718537126 3 0.21068373876152957 4 0.21253557602888187 5 0.22786112582765952 6 0.2305643825282772 7 0.20787405463175362 8 0.20287196546131928 9 0.18433230730196462 10-11 0.17295521512495537 12-13 0.19964721435782648 14-15 0.19712488428677766 16-17 0.19861486829499214 18-19 0.19085630870936096 20-21 0.1916013007134682 22-23 0.15936443242145604 24-25 0.46449187181795293 26-27 0.32670027928685963 28-29 0.2723903621874413 30-31 0.276381390780873 32-33 0.317973230095889 34-35 0.3131520675550235 36-37 0.3059895015726781 38-39 0.3304571675361433 40-41 0.33562954059323075 42-43 0.37212350605157 44-45 0.3384392247230067 46-47 0.3650035110408879 48-49 0.34020592004703243 50-51 0.36222575513985944 52-53 0.3514659420519677 54-55 0.327551698720125 56-57 0.36483322715423483 58-59 0.35141272833738857 60-61 0.31771780426590934 62-63 0.29567668368725064 64-65 0.30108319708848613 66-67 0.3100337438806889 68-69 0.3163768186585163 70-71 0.2936013488186662 72-73 0.29949742839402926 74-75 0.29841186861661584 76-77 0.30092355594474884 78-79 0.30324367390039714 80-81 0.3205274883956853 82-83 0.3376197335184887 84-85 0.33221322011725324 86-87 0.33620424871068494 88-89 0.3314150143985669 90-91 0.306404568546395 92-93 0.29032338400059426 94-95 0.2987843646186694 96-97 0.28777976844371383 98-99 0.3060320725443414 100-101 0.3287436859266966 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4698037.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.737644541143084 #Duplication Level Percentage of deduplicated Percentage of total 1 91.07346845201143 43.4763286409113 2 4.34707718687923 4.150384510803058 3 1.2281921399513156 1.758929994156653 4 0.6957901656040342 1.3286153440331385 5 0.42581805594174493 1.01637754968738 6 0.29096356666100653 0.8333949191811793 7 0.2496459880444063 0.8342258006870427 8 0.21297852110340526 0.8133674348266161 9 0.11875512455659568 0.5102180931169743 >10 1.0545550840057383 10.308952813506583 >50 0.13344826103310709 4.536722391284885 >100 0.13469008073814143 13.379401485723827 >500 0.023581292236753055 7.834176871753601 >1k 0.010765971520383047 8.021896330553762 >5k 2.25091528501581E-4 0.8117239736162548 >10k+ 4.501818388582217E-5 0.38528384615788996 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 17928 0.3816061899895637 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9479 0.20176512019807422 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8866 0.18871711738328156 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7449 0.15855558395985386 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6128 0.13043745717626318 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5849 0.12449880662923686 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 4801 0.1021916174776827 No Hit TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA 4729 0.10065906249780493 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005959936032857978 0.0 0.0 2 0.00634307477782742 0.0 0.0 3 0.006896497409449947 0.0 0.0 4 0.007237065182756117 0.0 0.0 5 0.00785434427187355 0.0 0.0 6 0.008578050790149162 0.0 0.0 7 0.009621039595899309 0.0 0.0 8 0.01049374451499637 0.0 0.0 9 0.011558018806578151 0.0 0.0 10-11 0.017113530608635054 0.0 0.0 12-13 0.02178569474867908 0.0 0.0 14-15 0.026117291115416927 0.0 2.1285485831635637E-5 16-17 0.029597468048889356 0.0 2.1285485831635637E-5 18-19 0.03349271195607868 0.0 2.1285485831635637E-5 20-21 0.037888164780311435 0.0 2.1285485831635637E-5 22-23 0.04248582971994473 0.0 3.192822874745346E-5 24-25 0.048243553637402174 0.0 4.2570971663271274E-5 26-27 0.05444827275732396 0.0 4.2570971663271274E-5 28-29 0.0612170572517841 0.0 6.385645749490691E-5 30-31 0.09851987117172556 0.0 6.385645749490691E-5 32-33 0.10735334779185435 0.0 7.449920041072472E-5 34-35 0.11457977023169465 0.0 8.514194332654255E-5 36-37 0.12216804593067274 0.0 9.578468624236037E-5 38-39 0.13042681443334736 0.0 1.064274291581782E-4 40-41 0.13943057494012925 0.0 1.064274291581782E-4 42-43 0.14956246619598781 0.0 1.3835565790563167E-4 44-45 0.16041806397012198 0.0 1.4899840082144948E-4 46-47 0.17196544003378433 0.0 1.4899840082144948E-4 48-49 0.1834702451257834 0.0 1.4899840082144948E-4 50-51 0.19633732131100712 0.0 1.4899840082144948E-4 52-53 0.2095130370407896 0.0 1.4899840082144948E-4 54-55 0.2245086618091769 0.0 1.4899840082144948E-4 56-57 0.24097298509994705 0.0 1.4899840082144948E-4 58-59 0.2593955730872277 0.0 1.4899840082144948E-4 60-61 0.2783396554773834 0.0 1.4899840082144948E-4 62-63 0.2991355751348914 0.0 1.4899840082144948E-4 64-65 0.3222196845193003 0.0 1.4899840082144948E-4 66-67 0.3468789198552502 0.0 1.4899840082144948E-4 68-69 0.37313456662857275 0.0 1.4899840082144948E-4 70-71 0.40148683375631145 0.0 1.4899840082144948E-4 72-73 0.43304256650171125 0.0 1.4899840082144948E-4 74-75 0.466939702688591 0.0 1.4899840082144948E-4 76-77 0.503156956831119 0.0 1.702838866530851E-4 78-79 0.544461442087408 0.0 1.702838866530851E-4 80-81 0.5906190181133099 0.0 1.702838866530851E-4 82-83 0.6416509703946562 0.0 1.809266295689029E-4 84-85 0.6993665652271364 0.0 1.9156937248472074E-4 86-87 0.7643724389569516 0.0 1.9156937248472074E-4 88-89 0.8360513124949847 0.0 1.9156937248472074E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 9745 0.0 89.880875 2 GAGCGGC 5850 0.0 88.46197 9 CGGGAGA 8590 0.0 87.54106 4 AGAGCGG 6260 0.0 85.92728 8 ATTCGGA 1080 0.0 85.23057 2 GAGAGCG 7685 0.0 85.05639 7 AAGCGGC 2445 0.0 82.45135 9 TCGGGAG 8605 0.0 82.096405 3 CGGAAAG 3380 0.0 80.145035 5 GTTCGGA 725 0.0 79.843445 2 ATCCGGA 1420 0.0 79.52544 2 GGATCGG 2585 0.0 78.749275 1 GATCGGG 11415 0.0 78.39992 1 ACCGGAA 1070 0.0 77.15176 3 GATCCGG 1500 0.0 76.23873 1 AAGCGTC 1060 0.0 76.07304 9 TCGGGAA 3030 0.0 75.31522 3 GAACCGG 1040 0.0 73.914825 1 TCGGAGA 1670 0.0 73.579865 4 AATCGGA 1535 0.0 72.33107 2 >>END_MODULE