FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_53115004-1.3420000003588a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_53115004-1.3420000003588a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4698037
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT179280.3816061899895637Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC94790.20176512019807422No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG88660.18871711738328156No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA74490.15855558395985386No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC61280.13043745717626318No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG58490.12449880662923686No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA48010.1021916174776827No Hit
TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA47290.10065906249780493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA97450.089.8808752
GAGCGGC58500.088.461979
CGGGAGA85900.087.541064
AGAGCGG62600.085.927288
ATTCGGA10800.085.230572
GAGAGCG76850.085.056397
AAGCGGC24450.082.451359
TCGGGAG86050.082.0964053
CGGAAAG33800.080.1450355
GTTCGGA7250.079.8434452
ATCCGGA14200.079.525442
GGATCGG25850.078.7492751
GATCGGG114150.078.399921
ACCGGAA10700.077.151763
GATCCGG15000.076.238731
AAGCGTC10600.076.073049
TCGGGAA30300.075.315223
GAACCGG10400.073.9148251
TCGGAGA16700.073.5798654
AATCGGA15350.072.331072
Produced by FastQC (version 0.11.2)