##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_52370180-2.342000000358cd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5321893 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.984927919445205 33.0 33.0 33.0 27.0 33.0 2 30.489124828327064 33.0 33.0 33.0 22.0 33.0 3 30.326506376584422 33.0 33.0 33.0 22.0 33.0 4 33.65973385785847 37.0 37.0 37.0 22.0 37.0 5 33.71293579183197 37.0 37.0 37.0 22.0 37.0 6 34.332963853275515 37.0 37.0 37.0 27.0 37.0 7 33.78582564512289 37.0 37.0 37.0 22.0 37.0 8 34.09369034664921 37.0 37.0 37.0 27.0 37.0 9 33.458446458807046 37.0 37.0 37.0 15.0 37.0 10-11 33.48371707210198 37.0 37.0 37.0 15.0 37.0 12-13 33.503747444753216 37.0 37.0 37.0 18.5 37.0 14-15 35.10570749919249 40.0 37.0 40.0 15.0 40.0 16-17 35.03417214513708 40.0 37.0 40.0 18.5 40.0 18-19 35.11220584479996 40.0 37.0 40.0 18.5 40.0 20-21 35.10887347791472 40.0 37.0 40.0 15.0 40.0 22-23 35.94694002679122 40.0 37.0 40.0 27.0 40.0 24-25 35.26179669527366 40.0 37.0 40.0 22.0 40.0 26-27 35.11452503836511 40.0 37.0 40.0 18.5 40.0 28-29 34.76649107751697 40.0 37.0 40.0 15.0 40.0 30-31 34.79849369387922 40.0 37.0 40.0 15.0 40.0 32-33 34.574520570030245 40.0 37.0 40.0 15.0 40.0 34-35 34.84600883557786 40.0 37.0 40.0 18.5 40.0 36-37 34.46384068601154 40.0 37.0 40.0 15.0 40.0 38-39 34.42567109109484 40.0 37.0 40.0 15.0 40.0 40-41 34.998839134871744 38.5 37.0 40.0 22.0 40.0 42-43 34.62582853507202 37.0 37.0 40.0 15.0 40.0 44-45 34.17639541043009 37.0 37.0 40.0 15.0 40.0 46-47 34.06831920521513 37.0 37.0 40.0 15.0 40.0 48-49 33.997650178235446 37.0 35.0 40.0 15.0 40.0 50-51 33.08481737607276 37.0 33.0 38.5 15.0 38.5 52-53 33.50906500750767 37.0 33.0 38.5 15.0 40.0 54-55 34.17848376508133 37.0 37.0 40.0 15.0 40.0 56-57 34.508053619266676 37.0 37.0 40.0 18.5 40.0 58-59 34.00872349368918 37.0 35.0 40.0 15.0 40.0 60-61 33.79157951879153 37.0 33.0 40.0 15.0 40.0 62-63 33.48896501677129 37.0 33.0 40.0 10.5 40.0 64-65 33.29821775823001 37.0 33.0 40.0 10.5 40.0 66-67 33.20181972843122 37.0 33.0 40.0 15.0 40.0 68-69 33.28687226518835 37.0 33.0 38.5 15.0 40.0 70-71 32.90499141940659 37.0 33.0 37.0 15.0 40.0 72-73 33.16958693081578 37.0 33.0 37.0 18.5 40.0 74-75 33.25933610465299 37.0 33.0 37.0 22.0 40.0 76-77 32.97713200923055 37.0 33.0 37.0 22.0 37.0 78-79 32.517131779988816 37.0 33.0 37.0 22.0 37.0 80-81 32.0251757222477 37.0 33.0 37.0 15.0 37.0 82-83 31.603960188602066 37.0 33.0 37.0 15.0 37.0 84-85 31.390926875079977 37.0 33.0 37.0 15.0 37.0 86-87 31.20885660421959 37.0 33.0 37.0 15.0 37.0 88-89 30.989187775853445 35.0 33.0 37.0 15.0 37.0 90-91 30.912019087944834 33.0 33.0 37.0 15.0 37.0 92-93 30.759712756344406 33.0 33.0 37.0 15.0 37.0 94-95 30.641915386122946 33.0 33.0 37.0 15.0 37.0 96-97 30.516389187080613 33.0 33.0 37.0 15.0 37.0 98-99 30.396879456238597 33.0 30.0 37.0 15.0 37.0 100-101 29.309902228398805 33.0 27.0 37.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.1619676736349156 2306 2 0.27721246743120176 2306 3 0.199482743492986 2306 4 0.46994634096295584 2306 5 0.40078945447385905 2306 6 0.04288777683894551 2306 7 0.6507675650828304 2306 8 0.056703321131791995 2306 9 0.4661074277900994 2306 10-11 0.3923582734239517 2306 12-13 0.42746061609270924 2306 14-15 0.8106433955767969 2306 16-17 0.6615902398990627 2306 18-19 0.728597512684992 2306 20-21 0.6580997964580888 2306 22-23 0.4385433352185899 2306 24-25 0.7515508533380668 2306 26-27 0.38966531787986725 2306 28-29 0.584997447288508 2306 30-31 0.620211124931366 2306 32-33 0.6424614350988307 2306 34-35 0.38643422398097016 2306 36-37 0.7197701690060825 2306 38-39 0.5740059700545643 2306 40-41 0.2337177665576533 2306 42-43 0.6856837418120278 2306 44-45 0.8493909779363591 2306 46-47 0.7550952110383022 2306 48-49 0.7482071156764647 2306 50-51 0.5945192140361613 2306 52-53 0.5547111240288984 2306 54-55 0.647343494139875 2306 56-57 0.5991738100596038 2306 58-59 0.9339840144225917 2306 60-61 0.7476746646481516 2306 62-63 0.8034052781082295 2306 64-65 0.7284476961177901 2306 66-67 0.6300142856863999 2306 68-69 0.4368299167279446 2306 70-71 0.5961315444520068 2306 72-73 0.18908703554436812 2306 74-75 0.2803065396182447 2306 76-77 0.29879339461423626 2306 78-79 0.3937562244912627 2306 80-81 0.6293458564231784 2306 82-83 0.7570780772143699 2306 84-85 0.734885880309168 2306 86-87 0.7520179004902268 2306 88-89 0.7341758839959667 2306 90-91 0.6266181629547454 2306 92-93 0.4921414254168148 2306 94-95 0.4851356098821924 2306 96-97 0.4631173794038048 2306 98-99 0.5279902940885428 2306 100-101 0.45805960439522053 2307 1 0.056662404380158904 2307 2 -0.05215493982219144 2307 3 -0.010098411644090532 2307 4 0.19492833371550233 2307 5 0.1245981915160641 2307 6 -0.09987071070077747 2307 7 0.4492441890766514 2307 8 -0.14864613085382672 2307 9 0.12897741175065391 2307 10-11 0.05207791728886235 2307 12-13 0.03925406368233553 2307 14-15 0.37163691296771617 2307 16-17 0.30019870664691695 2307 18-19 0.3021111722032117 2307 20-21 0.15943363226169538 2307 22-23 0.05765199503384366 2307 24-25 0.34428307257352486 2307 26-27 -0.043874479993426974 2307 28-29 0.07551904484694916 2307 30-31 0.12760755541226132 2307 32-33 0.2252237654222924 2307 34-35 0.01374824809954589 2307 36-37 0.2987523696773664 2307 38-39 0.09063854052416076 2307 40-41 -0.08782298551411571 2307 42-43 0.30746007323448765 2307 44-45 0.3818174823769098 2307 46-47 0.14435388793259563 2307 48-49 0.07181684478806005 2307 50-51 0.15567284741602094 2307 52-53 0.20799503334663427 2307 54-55 0.11377807770836057 2307 56-57 0.11332852758715006 2307 58-59 0.19035987951303923 2307 60-61 0.19486191403181863 2307 62-63 0.3465377409056316 2307 64-65 0.2778678441856286 2307 66-67 0.23739920638025325 2307 68-69 0.08194048494014083 2307 70-71 0.20049236142411075 2307 72-73 -0.1690710829396096 2307 74-75 -0.021016900248689296 2307 76-77 0.08209566389869849 2307 78-79 0.22922474708014562 2307 80-81 0.2725651995484881 2307 82-83 0.36444443373559565 2307 84-85 0.3730293145325909 2307 86-87 0.3228597114623959 2307 88-89 0.40175559855325105 2307 90-91 0.26723527318137386 2307 92-93 0.3024155811182432 2307 94-95 0.21295049537837585 2307 96-97 0.3025614195618225 2307 98-99 0.337408177327319 2307 100-101 0.17508545686202837 2201 1 0.020131657492925115 2201 2 -0.0721559139459238 2201 3 -0.10107326596831356 2201 4 -0.025393990543726375 2201 5 -0.03326265490890279 2201 6 -0.04629966165807531 2201 7 0.3566909222198831 2201 8 -0.2296810487617691 2201 9 0.10269876365727981 2201 10-11 -0.02502032308945701 2201 12-13 -0.002425259213119091 2201 14-15 0.2624171639374069 2201 16-17 0.21862691372790977 2201 18-19 0.18565816703149807 2201 20-21 0.10167318699858896 2201 22-23 0.05299546220586393 2201 24-25 0.3186512758465838 2201 26-27 0.06020195108289528 2201 28-29 0.1082392521581852 2201 30-31 0.1360903466309722 2201 32-33 0.17015882015034123 2201 34-35 0.030919946262876863 2201 36-37 0.16767472316029597 2201 38-39 0.16759442600263696 2201 40-41 -0.05185058863839487 2201 42-43 0.3038864781865769 2201 44-45 0.34875732802257886 2201 46-47 0.17456716568183595 2201 48-49 0.14281341764365862 2201 50-51 0.033569371122659675 2201 52-53 -0.021550194775429077 2201 54-55 0.25092866616764553 2201 56-57 0.22855502242429537 2201 58-59 0.29078816186722634 2201 60-61 0.24562875973062148 2201 62-63 0.26795468136010925 2201 64-65 0.2971962854991048 2201 66-67 0.19059781913949791 2201 68-69 0.12864573281035518 2201 70-71 0.20419751852370638 2201 72-73 -0.15407992754030886 2201 74-75 0.07763172446571787 2201 76-77 0.03153532136136761 2201 78-79 0.1306159728820333 2201 80-81 0.4039060255168536 2201 82-83 0.3016366438452245 2201 84-85 0.25045798210843984 2201 86-87 0.39353714810007645 2201 88-89 0.3907299569599374 2201 90-91 0.29137295228730764 2201 92-93 0.24372064124824533 2201 94-95 0.14299523865249597 2201 96-97 0.21513879598094476 2201 98-99 0.2539003688400463 2201 100-101 0.2185698421013953 1101 1 0.03516225503854642 1101 2 -0.13880461530458987 1101 3 -0.19828262580682576 1101 4 0.08417141422092556 1101 5 -0.057232567104975374 1101 6 -0.027970664868355755 1101 7 0.3214210747719619 1101 8 -0.10860852509164687 1101 9 0.006757236300011016 1101 10-11 -0.04947754209107558 1101 12-13 0.038704922139103815 1101 14-15 0.21276678309201458 1101 16-17 0.11599248304991505 1101 18-19 0.08557750575511847 1101 20-21 0.09335286677437438 1101 22-23 0.04330264810298701 1101 24-25 0.23907955278919246 1101 26-27 -0.029869224928255278 1101 28-29 -0.021389471186679998 1101 30-31 0.1463156764630682 1101 32-33 0.21696333446875826 1101 34-35 0.004532465352106385 1101 36-37 0.23162868049330143 1101 38-39 -0.05237852690442679 1101 40-41 -0.09120945670920122 1101 42-43 0.28494430439126717 1101 44-45 0.2868338852782557 1101 46-47 0.031239929770556785 1101 48-49 0.06549718422265727 1101 50-51 0.04286717453766187 1101 52-53 0.050810065824137496 1101 54-55 0.16427461173850588 1101 56-57 0.23874192597563137 1101 58-59 0.2624884973993531 1101 60-61 0.25548610509810743 1101 62-63 0.29253216183783337 1101 64-65 0.23961871684701208 1101 66-67 0.19887473044914117 1101 68-69 0.09259974231753176 1101 70-71 0.17538717321935593 1101 72-73 -0.15144059248975594 1101 74-75 0.16409889099577413 1101 76-77 0.19048173169979066 1101 78-79 0.17042492148367927 1101 80-81 0.3609367827154628 1101 82-83 0.31767151602110033 1101 84-85 0.31226272860194726 1101 86-87 0.3160345746088389 1101 88-89 0.3850937633165259 1101 90-91 0.33744546291388033 1101 92-93 0.4024017198136818 1101 94-95 0.33114134408502593 1101 96-97 0.23411623951935212 1101 98-99 0.3027871284428336 1101 100-101 0.3004146896667166 2304 1 0.16364052427277542 2304 2 0.08159119579705987 2304 3 0.19135540777528348 2304 4 0.3408815230192914 2304 5 0.3723376108251699 2304 6 0.21356830920693426 2304 7 0.6048614069850657 2304 8 0.16067621593290937 2304 9 0.443658817761694 2304 10-11 0.3599371734242851 2304 12-13 0.36016319412282627 2304 14-15 0.5809835864825104 2304 16-17 0.5338903635227226 2304 18-19 0.48693102724380566 2304 20-21 0.4766439103658371 2304 22-23 0.3480282101395531 2304 24-25 0.5304257190918023 2304 26-27 0.5126893676170639 2304 28-29 0.5520920570291779 2304 30-31 0.5605140907282689 2304 32-33 0.5263485586656174 2304 34-35 0.438181349187154 2304 36-37 0.5764996079988904 2304 38-39 0.5510341759891659 2304 40-41 0.3178069495708584 2304 42-43 0.4828211179792632 2304 44-45 0.5363891227449997 2304 46-47 0.4371327263867144 2304 48-49 0.5312543845805138 2304 50-51 0.5108247192203237 2304 52-53 0.4704347908505895 2304 54-55 0.6683523025349132 2304 56-57 0.5905263223466832 2304 58-59 0.7135759057809778 2304 60-61 0.6001812013120187 2304 62-63 0.5964998970495827 2304 64-65 0.6395649596017989 2304 66-67 0.6368530036471327 2304 68-69 0.5033171562232823 2304 70-71 0.6538472961808992 2304 72-73 0.4235416527794129 2304 74-75 0.5138355476847281 2304 76-77 0.615392924208642 2304 78-79 0.588520264726661 2304 80-81 0.7063631679179991 2304 82-83 0.7719186670949618 2304 84-85 0.7576517046397733 2304 86-87 0.6808742812444173 2304 88-89 0.6635794960150676 2304 90-91 0.7391561575748575 2304 92-93 0.786964621832901 2304 94-95 0.6837061171908765 2304 96-97 0.7647153072437192 2304 98-99 0.6827169544764438 2304 100-101 0.7464492263644331 2202 1 0.11035701846877544 2202 2 -0.08263789104946895 2202 3 0.03910805962555486 2202 4 0.05921760085266925 2202 5 0.033269287642312406 2202 6 0.1331615164476574 2202 7 0.3879846870918584 2202 8 0.13196372017041114 2202 9 0.07721854456484323 2202 10-11 0.05885635644336418 2202 12-13 0.09012313229182922 2202 14-15 0.24170947085162453 2202 16-17 0.14723075139564656 2202 18-19 0.17088435716611627 2202 20-21 0.19243027946706093 2202 22-23 0.16428612441922752 2202 24-25 0.28345939489140903 2202 26-27 0.20536739621957167 2202 28-29 0.13812086968402326 2202 30-31 0.1794776474201072 2202 32-33 0.21959739942447953 2202 34-35 0.056004430489657864 2202 36-37 0.160691990573973 2202 38-39 0.13274612315203882 2202 40-41 0.1494709348156178 2202 42-43 0.32734829141333677 2202 44-45 0.2432618290659505 2202 46-47 0.05956359859705884 2202 48-49 0.08181232879528721 2202 50-51 0.1301292132858407 2202 52-53 0.10524977273904312 2202 54-55 0.23401976032165805 2202 56-57 0.2938756637117308 2202 58-59 0.3045051468806079 2202 60-61 0.22825603560122687 2202 62-63 0.23613069255691244 2202 64-65 0.197654997526179 2202 66-67 0.2135210195853574 2202 68-69 0.22523645566658956 2202 70-71 0.22225730160020873 2202 72-73 0.15988631572854928 2202 74-75 0.41129600805066246 2202 76-77 0.42791321059062426 2202 78-79 0.4172672462121554 2202 80-81 0.4296090232783669 2202 82-83 0.33464715641123277 2202 84-85 0.3331333783660888 2202 86-87 0.27318874093595014 2202 88-89 0.4283874059423596 2202 90-91 0.3630602198614632 2202 92-93 0.37745646105283726 2202 94-95 0.31608038037055763 2202 96-97 0.2620593343651585 2202 98-99 0.2619862230024488 2202 100-101 0.31643440570370984 1102 1 0.17511774408749403 1102 2 0.0644982011075328 1102 3 0.12349333723369682 1102 4 0.10891970471801216 1102 5 0.20926428545230635 1102 6 0.20021678391005793 1102 7 0.3909841905983029 1102 8 0.1924053261216443 1102 9 0.14642481988131095 1102 10-11 0.2481086418456826 1102 12-13 0.23986002726429234 1102 14-15 0.29499274340569315 1102 16-17 0.2213101101111974 1102 18-19 0.23847905217075294 1102 20-21 0.23947535070657722 1102 22-23 0.1936244849529416 1102 24-25 0.23277063629242178 1102 26-27 0.2223044940371608 1102 28-29 0.3006215181547489 1102 30-31 0.26461547747864955 1102 32-33 0.2507814643140307 1102 34-35 0.18557530041878323 1102 36-37 0.32820492604164286 1102 38-39 0.2579696061831669 1102 40-41 0.2198037865687681 1102 42-43 0.21740719605663372 1102 44-45 0.20298333221587228 1102 46-47 0.20472081053156188 1102 48-49 0.35290675501098434 1102 50-51 0.25710913802493707 1102 52-53 0.2544167098194592 1102 54-55 0.44082049040395077 1102 56-57 0.47009612478225193 1102 58-59 0.42922967738780216 1102 60-61 0.3332765655750265 1102 62-63 0.38941842171514907 1102 64-65 0.39271046483943195 1102 66-67 0.38794343236995843 1102 68-69 0.3825256844024949 1102 70-71 0.425934034853654 1102 72-73 0.4098076070504959 1102 74-75 0.5595629417538035 1102 76-77 0.46289376691409245 1102 78-79 0.4211232533315794 1102 80-81 0.42569226010997596 1102 82-83 0.4251640293583243 1102 84-85 0.38300797979596624 1102 86-87 0.3885266359634265 1102 88-89 0.5285173786247661 1102 90-91 0.406852579361793 1102 92-93 0.3658103403178572 1102 94-95 0.46302182250677504 1102 96-97 0.5129003660759857 1102 98-99 0.5573719272956197 1102 100-101 0.3654302204997748 2305 1 0.1684335017122045 2305 2 -0.090303482929599 2305 3 -0.13578819940053322 2305 4 -0.16818934721330692 2305 5 -0.11248067190386735 2305 6 0.22919237212471444 2305 7 -0.017143228434044033 2305 8 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-0.8587027644157246 1213 64-65 -0.5367623882013746 1213 66-67 -0.487468711551756 1213 68-69 -0.3133347262578354 1213 70-71 -0.5978633256398069 1213 72-73 -0.3191942550527571 1213 74-75 -0.6109856500243396 1213 76-77 -0.6066648062597508 1213 78-79 -0.767058199240644 1213 80-81 -1.085312639586185 1213 82-83 -1.2472016111931445 1213 84-85 -1.231997387127759 1213 86-87 -1.1482150175467751 1213 88-89 -0.9847293652889988 1213 90-91 -1.0864464099001232 1213 92-93 -1.1373370990474534 1213 94-95 -1.037108444934411 1213 96-97 -1.0453251070993268 1213 98-99 -1.2178838454811363 1213 100-101 -1.0596209742279967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3963.0 3 640.0 4 727.0 5 527.0 6 902.0 7 6065.0 8 32578.0 9 95109.0 10 138083.0 11 105052.0 12 61380.0 13 39464.0 14 27121.0 15 20531.0 16 17386.0 17 15823.0 18 15904.0 19 15923.0 20 16780.0 21 18103.0 22 20053.0 23 20651.0 24 22190.0 25 24791.0 26 29076.0 27 34465.0 28 41991.0 29 51294.0 30 62851.0 31 81503.0 32 111099.0 33 158258.0 34 242218.0 35 416600.0 36 867842.0 37 1752967.0 38 751072.0 39 911.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.54800796463728 23.82723843402827 25.083388863110013 12.541364738224436 2 29.49444123269967 26.511756963787906 33.562676398724264 10.431125404788155 3 36.64124839821598 20.102966113264607 29.23925027563396 14.016535212885453 4 25.346720579218456 18.176993963461392 39.14174416344904 17.334541293871116 5 33.42084793741137 17.445169966042577 33.90103567318757 15.23294642335849 6 28.070916260674196 17.614344475109036 40.87085267553858 13.443886588678192 7 35.67777322303791 18.794088997943213 30.763719406851102 14.764418372167773 8 25.72803048219286 32.481758284465315 28.035660219051216 13.7545510142906 9 34.014801444451095 26.771536587376982 24.169498979087386 15.044162989084537 10-11 26.788667014081007 30.284077577742114 28.21841476096221 14.708840647214663 12-13 29.455939705846077 24.315542242379017 27.613641348973992 18.614876702800906 14-15 25.560516122375628 25.21842905802639 29.719070247688805 19.50198457190917 16-17 26.621406750860217 26.427805523259916 30.66745117605803 16.28333654982184 18-19 25.740571246650113 27.12864783471744 30.910739286783716 16.22004163184873 20-21 24.383591999161823 29.27900938309826 29.972313943743206 16.365084673996712 22-23 27.486601245500264 27.09247262611448 29.065778817838105 16.355147310547157 24-25 23.615571838626927 30.911831649185817 28.959735268775145 16.512861243412114 26-27 21.71483221692869 33.0050134049622 28.547762141430695 16.73239223667841 28-29 23.09833009261645 31.097880892419848 28.556479905969432 17.247309108994262 30-31 25.515509130804677 28.109236136562128 28.942498023849478 17.432756708783717 32-33 21.71338533813078 30.354122999181683 30.831184479921546 17.10130718276599 34-35 26.205641981098644 28.281045712208975 28.979365943476893 16.53394636321549 36-37 22.814454344090564 29.828202672800785 30.901399958174757 16.455943024933894 38-39 22.01007007082307 32.26872966149891 28.869305420008757 16.851894847669275 40-41 26.720448201453088 27.8445529816464 28.141968168495996 17.29303064840451 42-43 26.33791433930628 27.95502233605842 28.883244231148264 16.82381909348704 44-45 23.11991182028292 29.988447717957644 30.034076120997476 16.857564340761964 46-47 23.57237869837216 30.602951663042766 28.81007842070015 17.014591217884924 48-49 20.495041104155668 30.43620371068708 30.500669356663394 18.568085828493853 50-51 19.109660952092135 28.50952407086065 32.27742608215476 20.10338889489245 52-53 24.374978962385736 28.01579445150072 28.700897029729294 18.90832955638425 54-55 25.816611418693668 27.858995408790697 28.518153403665387 17.806239768850247 56-57 26.239748970901207 27.673372487792403 28.19386973678664 17.89300880451975 58-59 24.810548935478916 27.737400878386527 28.576366459969922 18.875683726164635 60-61 21.06757678280167 28.193033973364777 28.098657493872313 22.640731749961237 62-63 23.588600036618924 28.250332477415423 28.48166495304042 19.67940253292523 64-65 21.100238828345436 29.595754070603586 30.717596106899126 18.586410994151848 66-67 19.439060300796182 29.492900061769735 29.614799629117694 21.453240008316392 68-69 18.710820309959594 28.420606667251036 34.404808021286044 18.463765001503333 70-71 18.148088538164405 32.38792701828237 31.651547578483314 17.812436865069913 72-73 17.969756650759646 36.092668518564416 28.175819776681 17.76175505399494 74-75 18.40250576982754 35.94160353098085 27.79526010470172 17.860630594489894 76-77 18.813994002268892 35.4849234018003 27.99715512056623 17.70392747536458 78-79 18.849862054504374 35.216516705423345 28.331361337093558 17.60225990297873 80-81 18.272787434471276 35.41476286937124 28.139018683550503 18.173431012606983 82-83 17.999128731233004 34.733230268076824 28.330658658259296 18.936982342430873 84-85 18.12244386218196 33.8850466791949 29.020729078732227 18.971780379890923 86-87 18.181233699301092 33.48679456024007 29.639608079961732 18.692363660497108 88-89 18.59704446054109 33.72967647133128 29.046493318155065 18.62678574997257 90-91 19.050858779624143 32.99193944893761 28.88943571053415 19.0677660609041 92-93 18.729867489269022 33.55265333829478 28.758174676859806 18.959304495576397 94-95 18.496914980414832 33.70032159140533 28.701557031633868 19.10120639654597 96-97 19.114973270094165 33.68715343840362 28.341539751123925 18.85633354037829 98-99 19.44967682294187 32.83976142301763 28.104053371548677 19.60650838249183 100-101 19.411109124225582 32.776481693698734 27.85702644052369 19.955382741551993 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3134.0 1 2050.0 2 856.5 3 884.0 4 1011.0 5 989.0 6 1072.0 7 1356.0 8 1686.0 9 2001.5 10 2322.0 11 2576.0 12 2573.0 13 2461.0 14 2393.5 15 2424.5 16 2522.0 17 2584.5 18 2931.5 19 3531.5 20 4552.5 21 6147.0 22 8529.0 23 12037.0 24 16791.5 25 23046.0 26 31125.0 27 41242.0 28 53130.5 29 65639.5 30 78503.5 31 92255.5 32 108280.5 33 128630.5 34 154031.5 35 188729.5 36 238359.0 37 297452.5 38 350480.5 39 364617.0 40 354005.5 41 320946.0 42 272695.0 43 256587.5 44 241827.0 45 224763.0 46 230290.0 47 211476.0 48 157139.5 49 115224.5 50 93363.5 51 80192.0 52 70389.5 53 60417.5 54 52197.0 55 45726.0 56 39721.0 57 33968.0 58 28637.5 59 24069.5 60 20241.5 61 16877.5 62 13922.0 63 11300.5 64 9150.5 65 7316.5 66 5821.0 67 4664.5 68 3674.5 69 2871.0 70 2212.5 71 1687.0 72 1292.5 73 982.5 74 706.0 75 494.5 76 372.5 77 292.0 78 234.5 79 176.0 80 128.0 81 94.0 82 74.5 83 62.0 84 51.0 85 35.0 86 28.5 87 28.5 88 21.5 89 14.0 90 10.0 91 9.5 92 10.5 93 9.0 94 7.0 95 4.5 96 3.0 97 2.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2617489678954462 2 0.2490091401687332 3 0.21435981520109482 4 0.21730989330300327 5 0.23117713941261125 6 0.23442786241662508 7 0.21018836718438347 8 0.20648667682721167 9 0.1875084673818132 10-11 0.17498472817848837 12-13 0.20413788852951384 14-15 0.20053014970424998 16-17 0.2013945037978028 18-19 0.19418842130046582 20-21 0.19494003355572914 22-23 0.16020615220937362 24-25 0.47067838455226363 26-27 0.32909342596703844 28-29 0.27588867344758716 30-31 0.27918637221755493 32-33 0.32180278709098437 34-35 0.3171240008019703 36-37 0.3097488055471991 38-39 0.3337064461837169 40-41 0.3387516434471719 42-43 0.3744532255721789 44-45 0.3415889797107909 46-47 0.3681772632407303 48-49 0.3437310746382913 50-51 0.3661479101515194 52-53 0.3549770730076685 54-55 0.3311979402817757 56-57 0.36752899767056574 58-59 0.3546576377991816 60-61 0.32085387661871445 62-63 0.29864373447568376 64-65 0.30439356822844804 66-67 0.31391085841071964 68-69 0.3195855309379576 70-71 0.29683046990986106 72-73 0.3037359075050926 74-75 0.3017441350286449 76-77 0.30477876950927046 78-79 0.3073248560239749 80-81 0.32419855115463614 82-83 0.34073402077042886 84-85 0.33579217019207264 86-87 0.3392777720258562 88-89 0.33482446941342114 90-91 0.30978638615996223 92-93 0.2939461578802881 94-95 0.3023736102924279 96-97 0.2908175718677546 98-99 0.31040646627055446 100-101 0.33197773799661134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5321893.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.273534856980284 #Duplication Level Percentage of deduplicated Percentage of total 1 94.03473011229316 58.55875043414621 2 3.3598602959252375 4.184607545057688 3 0.7391101303135704 1.3808100138968806 4 0.3668046423078439 0.9136888671383878 5 0.2790025065025324 0.8687236156935161 6 0.17452100765887385 0.6520824032232121 7 0.141833662143356 0.618273845236421 8 0.07590439800050713 0.37814681397461486 9 0.09822024348214299 0.5504869580531688 >10 0.5660922067144591 7.0405328954471855 >50 0.07233130053186844 3.166454049199318 >100 0.07246508495210785 9.531877955757176 >500 0.01297243134767635 5.6693861298377195 >1k 0.0059700543139954735 5.635128159361892 >5k 1.8192351254354852E-4 0.851050313976553 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9283 0.1744304141402317 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8416 0.15813921850739954 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8160 0.15332890007371439 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7027 0.13203948294338125 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6706 0.1260077945948932 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5481 0.10298966927745447 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017287081871056032 0.0 0.0 2 0.0018226597190135164 0.0 0.0 3 0.001954191863684595 0.0 0.0 4 0.0020293530892109253 0.0 1.8790306381582642E-5 5 0.0022736270721715 0.0 1.8790306381582642E-5 6 0.002517901055132074 0.0 1.8790306381582642E-5 7 0.0028373362636189793 0.0 1.8790306381582642E-5 8 0.003325884229540128 0.0 1.8790306381582642E-5 9 0.0036453194380270328 0.0 1.8790306381582642E-5 10-11 0.006135035033586733 0.0 1.8790306381582642E-5 12-13 0.008493218484475354 0.0 4.697576595395661E-5 14-15 0.010907772854508724 0.0 5.6370919144747934E-5 16-17 0.011960030011877353 0.0 5.6370919144747934E-5 18-19 0.01314381931391706 0.0 5.6370919144747934E-5 20-21 0.014816156581877914 0.0 5.6370919144747934E-5 22-23 0.015905994352009706 0.0 5.6370919144747934E-5 24-25 0.017437404322108694 0.0 5.6370919144747934E-5 26-27 0.019156717356023506 0.0 8.45563787171219E-5 28-29 0.021355183202668673 0.0 9.395153190791322E-5 30-31 0.038811377831158955 0.0 9.395153190791322E-5 32-33 0.04179903654583059 0.0 9.395153190791322E-5 34-35 0.0437344381031336 0.0 9.395153190791322E-5 36-37 0.04599867002211431 0.0 9.395153190791322E-5 38-39 0.04883600628573329 9.395153190791321E-6 9.395153190791322E-5 40-41 0.051964592298266804 1.8790306381582642E-5 9.395153190791322E-5 42-43 0.05546898443843196 1.8790306381582642E-5 9.395153190791322E-5 44-45 0.05880426382116288 1.8790306381582642E-5 9.395153190791322E-5 46-47 0.0625717202506702 1.8790306381582642E-5 9.395153190791322E-5 48-49 0.0672411113864935 1.8790306381582642E-5 1.221369914802872E-4 50-51 0.07209840558613259 1.8790306381582642E-5 1.691127574342438E-4 52-53 0.07789521510485084 1.8790306381582642E-5 1.8790306381582644E-4 54-55 0.08432149988735212 1.8790306381582642E-5 1.9729821700661775E-4 56-57 0.09167790483574172 1.8790306381582642E-5 2.0669337019740909E-4 58-59 0.09952285775005247 1.8790306381582642E-5 2.0669337019740909E-4 60-61 0.1082509550642976 1.8790306381582642E-5 2.0669337019740909E-4 62-63 0.11884868786351022 1.8790306381582642E-5 2.0669337019740909E-4 64-65 0.13038593598180195 1.8790306381582642E-5 2.1608852338820042E-4 66-67 0.14335124738509397 1.8790306381582642E-5 2.3487882976978305E-4 68-69 0.15757550931595204 1.8790306381582642E-5 2.5366913615136567E-4 70-71 0.17396065648069212 1.8790306381582642E-5 2.6306428934215703E-4 72-73 0.19225301974316283 1.8790306381582642E-5 2.6306428934215703E-4 74-75 0.2124338087969826 1.8790306381582642E-5 2.8185459572373965E-4 76-77 0.23453120910172376 1.8790306381582642E-5 2.9124974891453097E-4 78-79 0.260001469401959 1.8790306381582642E-5 3.006449021053223E-4 80-81 0.2890512830678858 1.8790306381582642E-5 3.006449021053223E-4 82-83 0.3230429473121688 1.8790306381582642E-5 3.006449021053223E-4 84-85 0.3595149319988207 1.8790306381582642E-5 3.1943520848690495E-4 86-87 0.4019058631956711 1.8790306381582642E-5 3.1943520848690495E-4 88-89 0.4494359431878845 1.8790306381582642E-5 3.2883036167769626E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5345 0.0 89.00976 9 TCGGAGA 2095 0.0 84.699 4 CGGGAGA 7300 0.0 84.42616 4 AGAGCGG 5780 0.0 84.28445 8 GTTCGGA 1175 0.0 82.78268 2 GAGAGCG 7095 0.0 82.30667 7 TCGGGAG 7810 0.0 82.01127 3 CGGAAAG 2775 0.0 81.03972 5 ATTCGGA 1420 0.0 80.86312 2 CGGAGAG 3085 0.0 79.5093 5 AAGCGGC 1740 0.0 79.055695 9 ATCCGGA 1505 0.0 76.2961 2 ATCGGAG 1550 0.0 75.60596 3 ATCGGGA 9925 0.0 75.53532 2 TTCGGAG 1555 0.0 74.752625 3 GATCGGG 10245 0.0 74.38926 1 GGATCGG 3295 0.0 73.161896 1 AGAGCGT 11915 0.0 72.33787 8 GAGCGTC 11935 0.0 71.338905 9 CCGGAGA 1405 0.0 69.90122 4 >>END_MODULE