FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_52370180-2.342000000358cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_52370180-2.342000000358cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5321893
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC92830.1744304141402317No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC84160.15813921850739954No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG81600.15332890007371439No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG70270.13203948294338125No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA67060.1260077945948932No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA54810.10298966927745447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC53450.089.009769
TCGGAGA20950.084.6994
CGGGAGA73000.084.426164
AGAGCGG57800.084.284458
GTTCGGA11750.082.782682
GAGAGCG70950.082.306677
TCGGGAG78100.082.011273
CGGAAAG27750.081.039725
ATTCGGA14200.080.863122
CGGAGAG30850.079.50935
AAGCGGC17400.079.0556959
ATCCGGA15050.076.29612
ATCGGAG15500.075.605963
ATCGGGA99250.075.535322
TTCGGAG15550.074.7526253
GATCGGG102450.074.389261
GGATCGG32950.073.1618961
AGAGCGT119150.072.337878
GAGCGTC119350.071.3389059
CCGGAGA14050.069.901224
Produced by FastQC (version 0.11.2)