##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_52260024-1.3420000003582d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6635557 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.598715375363366 33.0 33.0 33.0 27.0 33.0 2 31.46335597750121 33.0 33.0 33.0 27.0 33.0 3 31.382967398215403 33.0 33.0 33.0 27.0 33.0 4 34.99818538217666 37.0 37.0 37.0 33.0 37.0 5 34.98974735655198 37.0 37.0 37.0 33.0 37.0 6 35.139387545009406 37.0 37.0 37.0 33.0 37.0 7 34.95489451752129 37.0 37.0 37.0 33.0 37.0 8 35.03092566306039 37.0 37.0 37.0 33.0 37.0 9 34.77701811618829 37.0 37.0 37.0 33.0 37.0 10-11 34.72754984999752 37.0 37.0 37.0 33.0 37.0 12-13 34.73775389164768 37.0 37.0 37.0 33.0 37.0 14-15 36.36570765649364 40.0 37.0 40.0 33.0 40.0 16-17 36.30870272081153 40.0 37.0 40.0 33.0 40.0 18-19 36.31301087459576 40.0 37.0 40.0 33.0 40.0 20-21 36.30975056050306 40.0 37.0 40.0 33.0 40.0 22-23 36.52388028616136 40.0 37.0 40.0 33.0 40.0 24-25 36.205142989503365 40.0 37.0 40.0 27.0 40.0 26-27 36.09070429204361 40.0 37.0 40.0 27.0 40.0 28-29 35.93789519101411 40.0 37.0 40.0 27.0 40.0 30-31 35.97001374564336 40.0 37.0 40.0 27.0 40.0 32-33 35.808745595885924 38.5 37.0 40.0 27.0 40.0 34-35 35.79361777767865 37.0 37.0 40.0 27.0 40.0 36-37 35.664703279619175 37.0 37.0 40.0 27.0 40.0 38-39 35.679998679839535 37.0 37.0 40.0 27.0 40.0 40-41 35.50282674084481 37.0 37.0 40.0 27.0 40.0 42-43 35.35174673354475 37.0 37.0 40.0 27.0 40.0 44-45 35.23653786713007 37.0 37.0 40.0 27.0 40.0 46-47 35.158886586310686 37.0 37.0 40.0 27.0 40.0 48-49 35.036978809766836 37.0 37.0 40.0 27.0 40.0 50-51 33.941518323179196 37.0 33.0 38.5 24.5 38.5 52-53 34.40609311320813 37.0 33.0 38.5 27.0 40.0 54-55 35.12642887401917 37.0 37.0 40.0 27.0 40.0 56-57 35.2220361455715 37.0 37.0 40.0 27.0 40.0 58-59 34.935128731468964 37.0 35.0 40.0 27.0 40.0 60-61 34.68190711043549 37.0 33.0 40.0 27.0 40.0 62-63 34.4127935303698 37.0 33.0 40.0 27.0 40.0 64-65 34.17015474360329 37.0 33.0 37.0 27.0 40.0 66-67 33.94912981683376 37.0 33.0 37.0 27.0 40.0 68-69 33.79018309992665 37.0 33.0 37.0 27.0 40.0 70-71 33.44292543941677 37.0 33.0 37.0 22.0 38.5 72-73 33.332626032750525 37.0 33.0 37.0 27.0 37.0 74-75 33.16389897637832 37.0 33.0 37.0 27.0 37.0 76-77 32.895743416867646 37.0 33.0 37.0 22.0 37.0 78-79 32.59292166430038 37.0 33.0 37.0 22.0 37.0 80-81 32.31155832132856 37.0 33.0 37.0 22.0 37.0 82-83 32.053828487947584 33.0 33.0 37.0 22.0 37.0 84-85 31.91388627962958 33.0 33.0 37.0 22.0 37.0 86-87 31.757044811761844 33.0 33.0 37.0 22.0 37.0 88-89 31.554289263131942 33.0 33.0 37.0 15.0 37.0 90-91 31.493471309190774 33.0 33.0 37.0 15.0 37.0 92-93 31.333560091488927 33.0 33.0 37.0 15.0 37.0 94-95 31.191623174964814 33.0 33.0 37.0 15.0 37.0 96-97 31.03699418149825 33.0 33.0 37.0 15.0 37.0 98-99 30.88618189852035 33.0 33.0 37.0 15.0 37.0 100-101 29.623416165364866 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.33031706247612647 2306 2 0.2960740207680175 2306 3 0.2258517631781558 2306 4 0.34585280449195466 2306 5 0.2964163004531031 2306 6 0.2939825694029352 2306 7 0.43609578691601314 2306 8 0.3237344607039816 2306 9 0.4614851354711291 2306 10-11 0.3899057954885734 2306 12-13 0.3998399106677297 2306 14-15 0.5466119295866108 2306 16-17 0.575933275401681 2306 18-19 0.5353464376195021 2306 20-21 0.5160546370488674 2306 22-23 0.38183847001516824 2306 24-25 0.5885441343616904 2306 26-27 0.5874344246546386 2306 28-29 0.5585120739954732 2306 30-31 0.5201510629800552 2306 32-33 0.5668240282500676 2306 34-35 0.4048474946775684 2306 36-37 0.4663767238399217 2306 38-39 0.5062520258392098 2306 40-41 0.48774200062185 2306 42-43 0.49638156858870985 2306 44-45 0.4951241385932903 2306 46-47 0.46235534881048324 2306 48-49 0.4734835232999757 2306 50-51 0.5048589762487055 2306 52-53 0.4940829726020155 2306 54-55 0.5603774789596301 2306 56-57 0.5805256354147943 2306 58-59 0.6047344662452048 2306 60-61 0.5837796855864994 2306 62-63 0.510206668015492 2306 64-65 0.5760220830903364 2306 66-67 0.4927970308828975 2306 68-69 0.4859536207127846 2306 70-71 0.5192765986110217 2306 72-73 0.3499017775444955 2306 74-75 0.5505704735522627 2306 76-77 0.506319708705874 2306 78-79 0.4624006246554231 2306 80-81 0.4825000928756964 2306 82-83 0.5863644553740244 2306 84-85 0.6519868903011172 2306 86-87 0.5559394099840773 2306 88-89 0.5225874628192457 2306 90-91 0.44513117278180303 2306 92-93 0.37753059325086724 2306 94-95 0.3163842004740012 2306 96-97 0.22443121336632998 2306 98-99 0.2519828495924372 2306 100-101 0.2477084586897753 2307 1 0.2972942993447738 2307 2 0.2689668184387024 2307 3 0.2737592302860179 2307 4 0.40588819522823627 2307 5 0.3843692217229062 2307 6 0.33934787098738894 2307 7 0.4817920616843523 2307 8 0.2921155914740581 2307 9 0.41809589336520503 2307 10-11 0.39629798835657937 2307 12-13 0.2499707083552849 2307 14-15 0.5023276984070222 2307 16-17 0.4920966173794312 2307 18-19 0.46752882417010255 2307 20-21 0.34178298898650183 2307 22-23 0.410345385935706 2307 24-25 0.45271791714356624 2307 26-27 0.4287750463012898 2307 28-29 0.42225561081403384 2307 30-31 0.373478340616316 2307 32-33 0.38651827499268876 2307 34-35 0.27552482381985755 2307 36-37 0.34400211205267084 2307 38-39 0.26508258597777257 2307 40-41 0.35019907573934006 2307 42-43 0.42652118327916355 2307 44-45 0.43855961339599503 2307 46-47 0.3464291531458912 2307 48-49 0.22128053817498028 2307 50-51 0.2721904081161668 2307 52-53 0.3333551077280532 2307 54-55 0.3898529996000093 2307 56-57 0.3567869193759847 2307 58-59 0.35123721489504334 2307 60-61 0.3578673424727796 2307 62-63 0.3374466623087926 2307 64-65 0.31723518610009194 2307 66-67 0.35174926105921145 2307 68-69 0.29097484441614085 2307 70-71 0.2731714588237466 2307 72-73 0.13765477260252368 2307 74-75 0.31462203020006996 2307 76-77 0.3325835238640664 2307 78-79 0.36283877102150797 2307 80-81 0.31032366230835606 2307 82-83 0.3590151977434246 2307 84-85 0.4211438137768937 2307 86-87 0.4019556894159564 2307 88-89 0.42955201302170565 2307 90-91 0.42174316175805515 2307 92-93 0.4072479774351798 2307 94-95 0.3430432467036262 2307 96-97 0.34174941397673564 2307 98-99 0.267994032113986 2307 100-101 0.289137480474178 2201 1 0.2874951113921398 2201 2 0.2358594114691357 2201 3 0.23077710765500115 2201 4 0.30197477011844853 2201 5 0.2943517042021355 2201 6 0.281533520910358 2201 7 0.42668581119448845 2201 8 0.2764434295080065 2201 9 0.36645825200064763 2201 10-11 0.3733575564434588 2201 12-13 0.3466190932026336 2201 14-15 0.4669498953114015 2201 16-17 0.4236444439207645 2201 18-19 0.4373700470997548 2201 20-21 0.4799518205883615 2201 22-23 0.37139874523354166 2201 24-25 0.48626272084442235 2201 26-27 0.4403928534327477 2201 28-29 0.46373120284228975 2201 30-31 0.3524608701515106 2201 32-33 0.4518803800166893 2201 34-35 0.35996727415957963 2201 36-37 0.3016321319388666 2201 38-39 0.40113236555810516 2201 40-41 0.31598067529856877 2201 42-43 0.2737576413738765 2201 44-45 0.3682079703850505 2201 46-47 0.29829221928073935 2201 48-49 0.3480038726356298 2201 50-51 0.32861395494933987 2201 52-53 0.3851181113766202 2201 54-55 0.4660678914846059 2201 56-57 0.5120176877406877 2201 58-59 0.46658486031420665 2201 60-61 0.5033234432279698 2201 62-63 0.5135825658504203 2201 64-65 0.4842959825496962 2201 66-67 0.4234864026923262 2201 68-69 0.4776740179488641 2201 70-71 0.5064341504739573 2201 72-73 0.39400216744146377 2201 74-75 0.4116006187261192 2201 76-77 0.37130910257663885 2201 78-79 0.3704173252515588 2201 80-81 0.4622515278252948 2201 82-83 0.4439151446394689 2201 84-85 0.3935161351414109 2201 86-87 0.4749935820247231 2201 88-89 0.6171556245524883 2201 90-91 0.5921610439769829 2201 92-93 0.4454435450520755 2201 94-95 0.32600186083909577 2201 96-97 0.4581699642399357 2201 98-99 0.5511505338985749 2201 100-101 0.3125823927357345 1101 1 0.263514035164512 1101 2 0.31007388914577305 1101 3 0.2997890671158565 1101 4 0.3840339605740013 1101 5 0.3884360437897314 1101 6 0.3825195881591057 1101 7 0.4980093111016046 1101 8 0.37224550600397066 1101 9 0.3787007107700262 1101 10-11 0.3501348181934034 1101 12-13 0.39099401837859915 1101 14-15 0.5793752508545751 1101 16-17 0.5246893135721322 1101 18-19 0.5094379150791966 1101 20-21 0.5053146129181201 1101 22-23 0.5016532336435588 1101 24-25 0.577058865084517 1101 26-27 0.5546227541489941 1101 28-29 0.5720220443804678 1101 30-31 0.5763580608960339 1101 32-33 0.643610427284834 1101 34-35 0.5874451035401407 1101 36-37 0.5970052200557774 1101 38-39 0.4471268750220574 1101 40-41 0.48841616975643376 1101 42-43 0.5110983394563249 1101 44-45 0.6405501339865154 1101 46-47 0.4541747630915012 1101 48-49 0.5735232732177167 1101 50-51 0.44763796056371774 1101 52-53 0.5432360712090158 1101 54-55 0.5508018729488882 1101 56-57 0.6527079760970409 1101 58-59 0.6225579608157901 1101 60-61 0.6570880106934425 1101 62-63 0.7589426996048303 1101 64-65 0.680177221842122 1101 66-67 0.6486614599714144 1101 68-69 0.5643795064208064 1101 70-71 0.5994284906807792 1101 72-73 0.528869069416821 1101 74-75 0.737943863921906 1101 76-77 0.6583131275936651 1101 78-79 0.4826204338031701 1101 80-81 0.6341691945538841 1101 82-83 0.7296798442080714 1101 84-85 0.6656931533262309 1101 86-87 0.651751804412072 1101 88-89 0.7483019741716674 1101 90-91 0.6704273886422847 1101 92-93 0.6043924995797063 1101 94-95 0.606188390448775 1101 96-97 0.605858349485672 1101 98-99 0.5886593001792555 1101 100-101 0.4664820026187009 2304 1 0.3160729641411386 2304 2 0.28305941217731245 2304 3 0.25146343365393875 2304 4 0.4350566668745799 2304 5 0.45013924324513965 2304 6 0.40471563989498094 2304 7 0.4384931776775005 2304 8 0.34156343959447355 2304 9 0.429604787617464 2304 10-11 0.45988226543642696 2304 12-13 0.5000420480283978 2304 14-15 0.6247869746273622 2304 16-17 0.6561813035880135 2304 18-19 0.6057206470964402 2304 20-21 0.5229152785258648 2304 22-23 0.6035452539143336 2304 24-25 0.7110646588973921 2304 26-27 0.7136403776267954 2304 28-29 0.6209684024471827 2304 30-31 0.5443669407084499 2304 32-33 0.6997097541133641 2304 34-35 0.6614492484610963 2304 36-37 0.6540701168109422 2304 38-39 0.5857716068880308 2304 40-41 0.5773282641517099 2304 42-43 0.5402847550073346 2304 44-45 0.6064725755567437 2304 46-47 0.5011898300304622 2304 48-49 0.5731260869231818 2304 50-51 0.4865205715613783 2304 52-53 0.5994701382236158 2304 54-55 0.7166944226803693 2304 56-57 0.6890733644942841 2304 58-59 0.7569605476095234 2304 60-61 0.6945983351772256 2304 62-63 0.6187862666572457 2304 64-65 0.6514985137262457 2304 66-67 0.7354527011785876 2304 68-69 0.5989877508803758 2304 70-71 0.6598333465918316 2304 72-73 0.5794373017744334 2304 74-75 0.6341996993352623 2304 76-77 0.7715184827105261 2304 78-79 0.7801019404970688 2304 80-81 0.7787502701154878 2304 82-83 0.7383385538366909 2304 84-85 0.7896054897606888 2304 86-87 0.5915112174670583 2304 88-89 0.6350859088210825 2304 90-91 0.6996541642265832 2304 92-93 0.6502437967584349 2304 94-95 0.6481888140173346 2304 96-97 0.7568835669427472 2304 98-99 0.6911629232032332 2304 100-101 0.6435658691946031 2202 1 0.22333082838559548 2202 2 0.21532041715281736 2202 3 0.24293014044405226 2202 4 0.3100219727525726 2202 5 0.30391045778775094 2202 6 0.3349117968088251 2202 7 0.3735830318015019 2202 8 0.26990437398387 2202 9 0.32784875376184175 2202 10-11 0.2983533721716185 2202 12-13 0.32336935310586057 2202 14-15 0.41023268088282094 2202 16-17 0.40134092576537483 2202 18-19 0.3537361433173345 2202 20-21 0.32654143511833666 2202 22-23 0.3078863285410236 2202 24-25 0.40504241667836993 2202 26-27 0.46997442870238615 2202 28-29 0.46769890142899584 2202 30-31 0.3830403689551716 2202 32-33 0.46792161176082914 2202 34-35 0.3621441534649321 2202 36-37 0.4157583839162413 2202 38-39 0.4165906511124433 2202 40-41 0.36375782266718204 2202 42-43 0.3848974517412671 2202 44-45 0.45198330224886973 2202 46-47 0.3876089437892034 2202 48-49 0.3915604251930631 2202 50-51 0.3774652596356489 2202 52-53 0.4110193366798356 2202 54-55 0.5276914367946759 2202 56-57 0.4851946346935634 2202 58-59 0.4588697468837921 2202 60-61 0.4010604988633588 2202 62-63 0.5004029835269179 2202 64-65 0.4987990919129146 2202 66-67 0.5947629236007685 2202 68-69 0.6354333782523582 2202 70-71 0.5880701986151919 2202 72-73 0.5034137156146841 2202 74-75 0.5957870978458217 2202 76-77 0.45171975244235085 2202 78-79 0.4952336174721452 2202 80-81 0.49179336094542947 2202 82-83 0.5110051367505264 2202 84-85 0.5079465556225529 2202 86-87 0.5136320435815804 2202 88-89 0.6345943478923601 2202 90-91 0.5918292154278042 2202 92-93 0.6257490162151917 2202 94-95 0.540908745534793 2202 96-97 0.4772372570826029 2202 98-99 0.5076441650044607 2202 100-101 0.6713018111363631 1102 1 0.12183842096002806 1102 2 0.10321857319254946 1102 3 0.1183761428945651 1102 4 0.126192298377525 1102 5 0.2329085252831291 1102 6 0.18727272623441849 1102 7 0.22807123578779454 1102 8 0.1086231192786542 1102 9 0.14486308247549573 1102 10-11 0.2261348181934082 1102 12-13 0.1868625589500681 1102 14-15 0.24470033042400274 1102 16-17 0.1967647990350656 1102 18-19 0.2508931237477938 1102 20-21 0.24943774705966604 1102 22-23 0.28228167837409046 1102 24-25 0.24942330380711297 1102 26-27 0.36285026907943774 1102 28-29 0.22003993945610745 1102 30-31 0.3051579464590972 1102 32-33 0.27460415471036725 1102 34-35 0.2146477069803936 1102 36-37 0.2657993408582726 1102 38-39 0.27040821107326707 1102 40-41 0.25409440170579245 1102 42-43 0.22276735923244928 1102 44-45 0.3021793154048211 1102 46-47 0.22285636377812068 1102 48-49 0.17714185491495016 1102 50-51 0.17020621146669868 1102 52-53 0.2330911225268224 1102 54-55 0.31865908426383527 1102 56-57 0.33797065892792233 1102 58-59 0.4073848678000118 1102 60-61 0.36895626517257796 1102 62-63 0.3018025644262039 1102 64-65 0.32015706663703014 1102 66-67 0.41736031202591306 1102 68-69 0.38852379709062035 1102 70-71 0.4050714045312205 1102 72-73 0.4320819864858052 1102 74-75 0.46705072656182267 1102 76-77 0.45213760994534624 1102 78-79 0.39300322534914045 1102 80-81 0.42335451801702817 1102 82-83 0.4019457098161894 1102 84-85 0.38013223353937065 1102 86-87 0.33873415966722575 1102 88-89 0.4619926082238024 1102 90-91 0.39451034111519334 1102 92-93 0.3684593021402307 1102 94-95 0.4311389608453702 1102 96-97 0.42259772651960503 1102 98-99 0.5296535139617511 1102 100-101 0.5268436376364001 2305 1 0.2176007350813869 2305 2 0.16983806204685337 2305 3 0.18209433173209177 2305 4 0.2041551298729729 2305 5 0.1546904078795066 2305 6 0.3372240769371615 2305 7 0.2371320043684335 2305 8 0.4202954090241988 2305 9 0.3208429108254407 2305 10-11 0.31388419475201346 2305 12-13 0.25921818019627096 2305 14-15 0.37442750909784905 2305 16-17 0.29081944657296077 2305 18-19 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1213 66-67 -0.8925009331909735 1213 68-69 -0.845524767083468 1213 70-71 -0.9043197999175092 1213 72-73 -0.8772296485319018 1213 74-75 -0.9120728027447669 1213 76-77 -0.984627043346503 1213 78-79 -0.9669543525216184 1213 80-81 -1.2125590105743242 1213 82-83 -1.218344942116719 1213 84-85 -1.1773410347079576 1213 86-87 -1.1084755460152778 1213 88-89 -0.9833364873667989 1213 90-91 -1.0629110728961813 1213 92-93 -1.1415476713604669 1213 94-95 -0.9803590454486653 1213 96-97 -1.0496647274374098 1213 98-99 -1.138994118624165 1213 100-101 -0.9789957751590777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5091.0 3 680.0 4 653.0 5 587.0 6 999.0 7 5566.0 8 22731.0 9 55513.0 10 69973.0 11 53382.0 12 34608.0 13 24682.0 14 19350.0 15 17162.0 16 16761.0 17 17588.0 18 19019.0 19 20950.0 20 23683.0 21 26766.0 22 29747.0 23 32557.0 24 36606.0 25 42714.0 26 49887.0 27 59280.0 28 73110.0 29 89917.0 30 113074.0 31 147966.0 32 201898.0 33 289741.0 34 435884.0 35 739740.0 36 1413854.0 37 2030561.0 38 412855.0 39 422.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.39265700923974 25.284874854512296 23.51502820153403 15.80743993471393 2 30.62491020276386 24.24293470435993 31.63174212001398 13.500412972862236 3 36.705537271560814 20.450985402606314 25.943372575457722 16.900104750375146 4 28.97703305650447 19.016812259950804 33.499635041402 18.506519642142727 5 32.450482075666926 17.83803828510059 32.06122655517172 17.650253084060765 6 27.233792058353302 18.5166873914667 37.193115861903955 17.056404688276046 7 33.18392456266598 19.099892700540877 29.238294655943264 18.477888080849876 8 28.425443415618595 27.39451682602427 26.000875263741914 18.179164494615225 9 30.148836655929717 25.535169072993646 25.229925441257244 19.086068829819393 10-11 25.17901532212945 28.43405826135029 28.699365671630524 17.68756074488973 12-13 26.23992245416442 25.346443480272562 28.224369192594306 20.18926487296871 14-15 24.680056755983173 25.83877063635151 28.97590301863279 20.505269589032533 16-17 24.656383606680656 26.615390794987164 29.82540915270459 18.902816445627586 18-19 24.087036147714606 26.7159574147626 29.93708185715387 19.259924580368924 20-21 23.574565691611564 27.8254072200292 29.178352988499828 19.42167409985941 22-23 24.742668668400928 27.05907329927809 28.62913706757604 19.569120964744943 24-25 23.184658046486952 27.979832377094603 29.009669084669675 19.82584049174877 26-27 22.423838173809596 28.65382324142265 29.037202750849282 19.885135833918472 28-29 22.592748839413975 28.541654718318593 28.53388687526952 20.33170956699791 30-31 23.234408054834585 27.932703889436144 28.67523288285688 20.157655172872392 32-33 22.070423091013307 27.679583221385894 29.474731277008186 20.775262410592614 34-35 21.164590280919665 27.84368464638699 31.13122674767349 19.860498325019854 36-37 21.417485820334363 28.266304385620387 29.942783964452797 20.373425829592453 38-39 21.217388753421513 27.91741741616865 28.83332487791114 22.031868952498698 40-41 21.866926124526163 27.57984824195272 29.1069859905761 21.446239642945013 42-43 22.808553549848444 27.786117836904246 29.579106584896685 19.82622202835062 44-45 21.75175922682941 28.6187929523253 29.885631352679535 19.743816468165754 46-47 22.301748545450337 28.77827055659905 29.13812061428488 19.781860283665736 48-49 21.270390475878653 28.716630183617024 29.548064127393985 20.464915213110334 50-51 20.816510578680575 28.3392589716337 29.38514314445788 21.45908730522784 52-53 22.640728088688544 27.89399645054647 28.542488628529757 20.922786832235232 54-55 23.253042417688167 27.91518216401248 28.49073975787675 20.34103566042261 56-57 23.596310643909852 27.52497010409621 28.056066268579006 20.82265298341493 58-59 22.855297002272703 27.554395596126913 28.256776540405376 21.33353086119501 60-61 21.33815844082603 27.8749367819698 28.006369807144377 22.78053497005979 62-63 22.28805976364844 27.959044529562387 28.25962529210638 21.49327041468279 64-65 21.608245608651348 28.465396412969156 29.084705936062427 20.841652042317065 66-67 21.6398643071731 28.356397692130404 28.388327550456015 21.615410450240482 68-69 21.597232249300163 27.699581269225217 30.08646764040408 20.61671884107054 70-71 21.314529549812196 29.59340623544393 28.992000478869322 20.100063735874546 72-73 21.290912870127173 31.211458589217887 27.508966194894274 19.988662345760662 74-75 21.86694241248771 30.7411895899654 27.153150641901142 20.238717355645747 76-77 21.913326942848293 29.961256487675136 27.66283605412693 20.462580515349636 78-79 21.73707249822919 29.764889096198665 28.295244697217242 20.202793708354903 80-81 21.182523757910516 30.157938517280186 28.084710559955862 20.574827164853435 82-83 20.42115958808485 30.067579829126835 28.397372964196965 21.11388761859135 84-85 20.546552964907008 29.85405353991314 28.46058799877151 21.13880549640835 86-87 20.359975120627606 29.890043517730337 29.065245140908253 20.684736220733807 88-89 20.523579930862457 30.173711840505657 28.84034816312807 20.46236006550382 90-91 20.957557657660722 29.540722901948246 28.393151868972556 21.108567571418472 92-93 20.591099423615333 30.170564968826557 27.985994151129702 21.25234145642841 94-95 20.310093608715448 30.41627942694366 27.9882485719244 21.28537839241649 96-97 20.69423208189063 30.769142405945303 27.669140350336242 20.867485161827823 98-99 20.978698482302956 30.144445101231454 27.606957722754483 21.269898693711106 100-101 21.02684537930391 30.01509181693273 27.183075813754837 21.774986990008525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3540.0 1 2234.5 2 733.0 3 443.5 4 369.0 5 403.0 6 523.0 7 740.5 8 1022.0 9 1370.0 10 1714.5 11 2047.5 12 2215.0 13 2142.0 14 1912.0 15 1670.5 16 1492.5 17 1310.0 18 1244.0 19 1315.5 20 1471.5 21 1738.0 22 2172.0 23 2717.5 24 3375.5 25 4359.5 26 5921.0 27 8365.5 28 10924.5 29 13567.5 30 16932.5 31 21499.0 32 32984.5 33 55896.5 34 92186.0 35 144759.0 36 216169.5 37 303987.0 38 389487.0 39 425823.0 40 429910.5 41 413471.5 42 389730.5 43 401788.5 44 405345.5 45 396663.5 46 402988.5 47 370635.5 48 299560.5 49 248959.5 50 209664.0 51 180420.0 52 159507.0 53 131686.0 54 99445.0 55 71109.5 56 53097.5 57 41008.5 58 33303.0 59 26656.0 60 20645.0 61 16107.5 62 12410.5 63 9492.0 64 7493.0 65 5783.5 66 4269.5 67 3282.5 68 2539.0 69 1820.5 70 1288.0 71 974.5 72 778.5 73 564.0 74 411.5 75 337.5 76 254.0 77 204.5 78 170.0 79 134.0 80 98.5 81 79.5 82 75.5 83 66.0 84 57.0 85 47.0 86 35.5 87 30.5 88 30.5 89 23.5 90 17.5 91 21.0 92 15.5 93 11.0 94 10.0 95 5.5 96 5.5 97 5.0 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2607166210764221 2 0.24832881399406262 3 0.21250665166466054 4 0.2148726926767414 5 0.22947583752200457 6 0.2331228561520909 7 0.20950765700603582 8 0.20424811360975423 9 0.18571161396096816 10-11 0.1739567605251526 12-13 0.20207798682160366 14-15 0.198498784653647 16-17 0.2000434929577125 18-19 0.1920788262387016 20-21 0.19268917439786895 22-23 0.16027290549986986 24-25 0.4703297703568819 26-27 0.3277042153356531 28-29 0.27930586686242015 30-31 0.28198084953531405 32-33 0.3253683149734077 34-35 0.32099038558481224 36-37 0.3135079692631681 38-39 0.33823083729067505 40-41 0.34351298617433323 42-43 0.38015648121175055 44-45 0.346511980832958 46-47 0.37296040106354295 48-49 0.3485087988845548 50-51 0.37066217651359185 52-53 0.35920119441367165 54-55 0.33502839324566125 56-57 0.37225963095486936 58-59 0.3591484482764597 60-61 0.3246449393773575 62-63 0.3018661432642354 64-65 0.30815800391738024 66-67 0.3173584372796436 68-69 0.32434353287900325 70-71 0.3010071347439258 72-73 0.3079168787186969 74-75 0.30571661128071087 76-77 0.30915264536194925 78-79 0.311315236987641 80-81 0.32872146226759863 82-83 0.3456077613378952 84-85 0.34034821794161363 86-87 0.34435692436972515 88-89 0.33919533808540864 90-91 0.31285241012924764 92-93 0.2976313819623583 94-95 0.30684688564953927 96-97 0.2940672501193193 98-99 0.31442725908314856 100-101 0.33713070357168207 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6635557.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.92788388958922 #Duplication Level Percentage of deduplicated Percentage of total 1 86.1020599820519 30.934648136896065 2 6.234091827103881 4.479554546424507 3 2.099592312508432 2.2630172645783118 4 1.2289793230615818 1.7661850568664987 5 0.7665802531749527 1.3770803164060803 6 0.5234954071003722 1.128484932182125 7 0.30654758475861504 0.7709524222289073 8 0.2873213683684779 0.8258279009392453 9 0.2051343099021966 0.6633037501143418 >10 1.7366103482362223 12.717449411203665 >50 0.23521489475585045 5.876973152395026 >100 0.21762238240279405 17.100710186215302 >500 0.039660886042552884 9.754902486608023 >1k 0.016833692786881987 9.755027193368225 >5k 2.55427745312226E-4 0.5858832435734875 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 7854 0.11836233190371206 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7132 0.10748155731312382 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6909 0.104120874856474 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001582384116359787 0.0 0.0 2 0.0017632280153723342 0.0 0.0 3 0.002034493863891155 0.0 0.0 4 0.0022605487376568386 0.0 0.0 5 0.0027729397848590557 0.0 0.0 6 0.003164768232719574 0.0 0.0 7 0.003616877980250942 0.0 0.0 8 0.00406898772778231 0.0 0.0 9 0.004581378774984527 0.0 0.0 10-11 0.006924814299688782 0.0 0.0 12-13 0.00907233560046278 1.5070324917712259E-5 0.0 14-15 0.011551404049426447 1.5070324917712259E-5 0.0 16-17 0.013992796686095831 1.5070324917712259E-5 0.0 18-19 0.016923974882590866 1.5070324917712259E-5 0.0 20-21 0.019734590479744202 1.5070324917712259E-5 0.0 22-23 0.022914429037381487 1.5070324917712259E-5 0.0 24-25 0.02660665864222099 1.5070324917712259E-5 0.0 26-27 0.030291353084601638 1.5070324917712259E-5 0.0 28-29 0.034925477996798154 1.5070324917712259E-5 0.0 30-31 0.06206713317359794 1.5070324917712259E-5 0.0 32-33 0.06846448610116679 1.5070324917712259E-5 0.0 34-35 0.07367881852269523 1.5070324917712259E-5 0.0 36-37 0.07938293650404932 1.5070324917712259E-5 0.0 38-39 0.08592345751833644 1.5070324917712259E-5 0.0 40-41 0.09351890127686342 1.5070324917712259E-5 0.0 42-43 0.10173976351947545 1.5070324917712259E-5 0.0 44-45 0.11118885724288105 1.5070324917712259E-5 0.0 46-47 0.12191139342183333 1.5070324917712259E-5 0.0 48-49 0.13489447833844243 1.5070324917712259E-5 0.0 50-51 0.14820157524078234 1.5070324917712259E-5 0.0 52-53 0.16385210766782654 1.5070324917712259E-5 7.5351624588561295E-6 54-55 0.18089664514975912 1.5070324917712259E-5 1.5070324917712259E-5 56-57 0.1992598360619915 1.5070324917712259E-5 1.5070324917712259E-5 58-59 0.21906977816632425 1.5070324917712259E-5 1.5070324917712259E-5 60-61 0.240974495434219 1.5070324917712259E-5 1.5070324917712259E-5 62-63 0.26489110107862834 1.5070324917712259E-5 1.5070324917712259E-5 64-65 0.2915957168328145 1.5070324917712259E-5 1.5070324917712259E-5 66-67 0.32180418313036874 1.5070324917712259E-5 1.5070324917712259E-5 68-69 0.35513974184834823 1.5070324917712259E-5 3.0140649835424518E-5 70-71 0.39029127471891206 1.5070324917712259E-5 3.0140649835424518E-5 72-73 0.4294138382052931 1.5070324917712259E-5 3.0140649835424518E-5 74-75 0.47451178552154705 1.5070324917712259E-5 3.0140649835424518E-5 76-77 0.5249446278586711 1.5070324917712259E-5 3.0140649835424518E-5 78-79 0.5806972948917476 1.5070324917712259E-5 3.0140649835424518E-5 80-81 0.6417019701586468 1.5070324917712259E-5 3.0140649835424518E-5 82-83 0.709985612360801 1.5070324917712259E-5 3.767581229428065E-5 84-85 0.7876731373116077 1.5070324917712259E-5 4.521097475313678E-5 86-87 0.8726773050099637 1.5070324917712259E-5 4.521097475313678E-5 88-89 0.9661962062868272 1.5070324917712259E-5 4.521097475313678E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2385 0.0 74.187706 9 CGGGAGA 3150 0.0 70.79306 4 AGAGCGG 2665 0.0 70.13373 8 CGGAAAG 1410 0.0 68.64432 5 AAGCGGC 965 0.0 62.92079 9 TCGGGAA 1265 0.0 60.761868 3 GAACCGG 495 0.0 58.485653 1 TCGGGAG 3790 0.0 57.837482 3 GAGAGCG 3825 0.0 56.557156 7 ATTCGGA 455 0.0 55.27351 2 AACCGGA 545 0.0 52.2405 2 ACCGGAA 615 0.0 51.689972 3 GATCCGG 755 0.0 51.545605 1 GTTCGGA 480 0.0 51.4061 2 TTGTGTT 68645 0.0 48.84589 1 GGATCGG 1640 0.0 47.459675 1 AAGCGTC 490 0.0 46.468292 9 GGAAAGC 1715 0.0 46.19797 6 GATCGGG 6695 0.0 45.084915 1 TCGGAGA 990 0.0 45.05012 4 >>END_MODULE