FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_52260024-1.3420000003582d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_52260024-1.3420000003582d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6635557
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA78540.11836233190371206No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC71320.10748155731312382No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG69090.104120874856474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC23850.074.1877069
CGGGAGA31500.070.793064
AGAGCGG26650.070.133738
CGGAAAG14100.068.644325
AAGCGGC9650.062.920799
TCGGGAA12650.060.7618683
GAACCGG4950.058.4856531
TCGGGAG37900.057.8374823
GAGAGCG38250.056.5571567
ATTCGGA4550.055.273512
AACCGGA5450.052.24052
ACCGGAA6150.051.6899723
GATCCGG7550.051.5456051
GTTCGGA4800.051.40612
TTGTGTT686450.048.845891
GGATCGG16400.047.4596751
AAGCGTC4900.046.4682929
GGAAAGC17150.046.197976
GATCGGG66950.045.0849151
TCGGAGA9900.045.050124
Produced by FastQC (version 0.11.2)