##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_52150223-1.3420000003573b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3217964 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.574791389835312 33.0 33.0 33.0 27.0 33.0 2 31.342380772438723 33.0 33.0 33.0 27.0 33.0 3 31.26337988865009 33.0 33.0 33.0 27.0 33.0 4 34.807719725888795 37.0 37.0 37.0 33.0 37.0 5 34.81222568058561 37.0 37.0 37.0 33.0 37.0 6 35.088001295228906 37.0 37.0 37.0 33.0 37.0 7 34.782417702621906 37.0 37.0 37.0 33.0 37.0 8 34.968696666587945 37.0 37.0 37.0 33.0 37.0 9 34.606533199252695 37.0 37.0 37.0 33.0 37.0 10-11 34.58388238650277 37.0 37.0 37.0 33.0 37.0 12-13 34.575047763119784 37.0 37.0 37.0 33.0 37.0 14-15 36.196753444103166 40.0 37.0 40.0 33.0 40.0 16-17 36.1441151920904 40.0 37.0 40.0 30.0 40.0 18-19 36.154076304147594 40.0 37.0 40.0 27.0 40.0 20-21 36.13345472478872 40.0 37.0 40.0 27.0 40.0 22-23 36.48721039141519 40.0 37.0 40.0 33.0 40.0 24-25 36.06938719637634 40.0 37.0 40.0 27.0 40.0 26-27 35.99122752771628 40.0 37.0 40.0 27.0 40.0 28-29 35.73608623340721 40.0 37.0 40.0 27.0 40.0 30-31 35.732292219552484 40.0 37.0 40.0 27.0 40.0 32-33 35.59391139863591 40.0 37.0 40.0 27.0 40.0 34-35 35.58745063648941 38.5 37.0 40.0 27.0 40.0 36-37 35.46351108962064 37.0 37.0 40.0 27.0 40.0 38-39 35.347380517619214 37.0 37.0 40.0 27.0 40.0 40-41 35.33003259203645 37.0 37.0 40.0 27.0 40.0 42-43 35.18593620065358 37.0 37.0 40.0 22.0 40.0 44-45 35.048491841425204 37.0 37.0 40.0 22.0 40.0 46-47 34.95793660214969 37.0 37.0 40.0 22.0 40.0 48-49 34.85087558468646 37.0 37.0 40.0 22.0 40.0 50-51 33.86471321618265 37.0 35.0 38.5 22.0 38.5 52-53 34.29418290571306 37.0 35.0 38.5 22.0 40.0 54-55 34.965025090398775 37.0 37.0 40.0 22.0 40.0 56-57 35.13039657994931 37.0 37.0 40.0 24.5 40.0 58-59 34.82403205878002 37.0 37.0 40.0 22.0 40.0 60-61 34.669799133862284 37.0 33.0 40.0 22.0 40.0 62-63 34.41042115449396 37.0 33.0 40.0 22.0 40.0 64-65 34.235319133464515 37.0 33.0 40.0 22.0 40.0 66-67 34.07675660759412 37.0 33.0 40.0 22.0 40.0 68-69 34.00050699759227 37.0 33.0 40.0 22.0 40.0 70-71 33.645978637424165 37.0 33.0 37.0 22.0 40.0 72-73 33.65286031789044 37.0 33.0 37.0 22.0 40.0 74-75 33.54723141713208 37.0 33.0 37.0 22.0 40.0 76-77 33.232923674721036 37.0 33.0 37.0 22.0 40.0 78-79 32.81848087797129 37.0 33.0 37.0 22.0 37.0 80-81 32.40158140364528 37.0 33.0 37.0 22.0 37.0 82-83 32.03810375131605 37.0 33.0 37.0 15.0 37.0 84-85 31.834226082081713 37.0 33.0 37.0 15.0 37.0 86-87 31.6249277493471 35.0 33.0 37.0 15.0 37.0 88-89 31.370499483524366 33.0 33.0 37.0 15.0 37.0 90-91 31.25179368072483 33.0 33.0 37.0 15.0 37.0 92-93 31.074230165408935 33.0 33.0 37.0 15.0 37.0 94-95 30.91826306944391 33.0 33.0 37.0 15.0 37.0 96-97 30.75695144507521 33.0 30.0 37.0 15.0 37.0 98-99 30.604261421196757 33.0 27.0 37.0 15.0 37.0 100-101 29.366368610711618 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.21665323513423473 2306 2 0.2066845769577661 2306 3 0.2529508739227744 2306 4 0.4320568629451671 2306 5 0.337024506662047 2306 6 0.3629247436080263 2306 7 0.504191688286248 2306 8 0.26163491001233297 2306 9 0.3896293660824668 2306 10-11 0.37976843818132267 2306 12-13 0.2843575505414577 2306 14-15 0.5443596803252149 2306 16-17 0.4282003971982604 2306 18-19 0.3464667617461217 2306 20-21 0.5271816024335294 2306 22-23 0.27764784332607206 2306 24-25 0.47371743875506667 2306 26-27 0.4077847232062837 2306 28-29 0.42755906530215526 2306 30-31 0.5166543084058262 2306 32-33 0.47023516168810175 2306 34-35 0.4504452453407879 2306 36-37 0.33111998797058106 2306 38-39 0.3172145618603395 2306 40-41 0.20914929839067042 2306 42-43 0.30972468041220225 2306 44-45 0.3607948892888686 2306 46-47 0.3458949511933227 2306 48-49 0.29612903967613136 2306 50-51 0.34800078638347287 2306 52-53 0.29640343065538843 2306 54-55 0.3258614855307016 2306 56-57 0.4148924629687514 2306 58-59 0.5939063074591502 2306 60-61 0.4266581185665004 2306 62-63 0.32668125559822414 2306 64-65 0.2761622717278769 2306 66-67 0.38573084085587084 2306 68-69 0.5188716044860939 2306 70-71 0.4697180848477416 2306 72-73 0.2929844243067734 2306 74-75 0.364131660499865 2306 76-77 0.22469627942378878 2306 78-79 0.28529260779036036 2306 80-81 0.33109342639563266 2306 82-83 0.4471932641171108 2306 84-85 0.45279154646862096 2306 86-87 0.4810185700433074 2306 88-89 0.5721442812098694 2306 90-91 0.23778383681133164 2306 92-93 0.23982444142560766 2306 94-95 0.12616270068857816 2306 96-97 0.13610379735127154 2306 98-99 0.19851554800965232 2306 100-101 0.27558087838957235 2307 1 0.41230702632682537 2307 2 0.376286103825624 2307 3 0.37484837900455403 2307 4 0.4741531429372543 2307 5 0.46219151292935834 2307 6 0.37262155296539134 2307 7 0.6986290725793083 2307 8 0.3459118521105111 2307 9 0.49777584772537864 2307 10-11 0.5395872449163264 2307 12-13 0.436132948181978 2307 14-15 0.7041837864200318 2307 16-17 0.6142720286449119 2307 18-19 0.697025528283433 2307 20-21 0.5162654307644345 2307 22-23 0.293950916159865 2307 24-25 0.5668632671499694 2307 26-27 0.4458504324145167 2307 28-29 0.4617880380029149 2307 30-31 0.48768372739420585 2307 32-33 0.5093950867877695 2307 34-35 0.380329721334995 2307 36-37 0.42441042774203197 2307 38-39 0.42047248598288434 2307 40-41 0.4415025830663737 2307 42-43 0.3919335473418073 2307 44-45 0.40107318313143736 2307 46-47 0.3486118922071668 2307 48-49 0.30715038325543276 2307 50-51 0.2964067287594574 2307 52-53 0.2547633032506269 2307 54-55 0.31969333643606745 2307 56-57 0.35892001311751187 2307 58-59 0.3483009544535065 2307 60-61 0.40210312721159625 2307 62-63 0.4343905400562491 2307 64-65 0.38647272656989173 2307 66-67 0.3179387803975118 2307 68-69 0.3487839632658023 2307 70-71 0.2738806504553395 2307 72-73 0.034145346909006946 2307 74-75 0.32162906228489163 2307 76-77 0.20441481411818785 2307 78-79 0.15443643302725008 2307 80-81 0.2083255014639107 2307 82-83 0.3699768017781295 2307 84-85 0.39042817058890833 2307 86-87 0.34548479304485014 2307 88-89 0.5643218146554574 2307 90-91 0.4657801305206064 2307 92-93 0.5226464850503447 2307 94-95 0.2926599237221197 2307 96-97 0.41834678400524083 2307 98-99 0.31157993747346424 2307 100-101 0.35131250087214383 2201 1 0.2932179814939815 2201 2 0.21800510369392612 2201 3 0.22706236504485133 2201 4 0.38653964981362776 2201 5 0.3527788739437838 2201 6 0.2549725021121958 2201 7 0.5217148814706221 2201 8 0.29365440662295583 2201 9 0.35187495784145995 2201 10-11 0.397286716253781 2201 12-13 0.3815355595115406 2201 14-15 0.6230254322349396 2201 16-17 0.5159121054044533 2201 18-19 0.4436198879620932 2201 20-21 0.4651262664651483 2201 22-23 0.3892845755958305 2201 24-25 0.458513973601157 2201 26-27 0.47039495483093674 2201 28-29 0.4080938909180887 2201 30-31 0.5168963776679405 2201 32-33 0.5509723260781172 2201 34-35 0.4036111918070304 2201 36-37 0.3305988837087668 2201 38-39 0.4706115185868782 2201 40-41 0.2868445204922949 2201 42-43 0.24535555550691868 2201 44-45 0.3910844712995569 2201 46-47 0.37506552605661625 2201 48-49 0.5057399266490279 2201 50-51 0.39467540597429007 2201 52-53 0.2500394870972542 2201 54-55 0.5121159654504197 2201 56-57 0.3966631320383698 2201 58-59 0.4852103480907388 2201 60-61 0.46279330462396473 2201 62-63 0.5194776470868945 2201 64-65 0.3554526524442565 2201 66-67 0.30123699498558665 2201 68-69 0.3433793765068174 2201 70-71 0.39751939144832704 2201 72-73 0.2837814916233228 2201 74-75 0.31808156731753456 2201 76-77 0.16965910888988844 2201 78-79 0.23755018013465445 2201 80-81 0.5055745748934442 2201 82-83 0.44387535877397255 2201 84-85 0.46518735633570785 2201 86-87 0.5681492710611487 2201 88-89 0.6702970183128194 2201 90-91 0.523252615610815 2201 92-93 0.48327034447502726 2201 94-95 0.5301975979832179 2201 96-97 0.6056878560049377 2201 98-99 0.5098780579934719 2201 100-101 0.6578202238605009 1101 1 0.2802626519298492 1101 2 0.2672648714004353 1101 3 0.24734722261606734 1101 4 0.31567821306212096 1101 5 0.3611343949784498 1101 6 0.13988787575068073 1101 7 0.5120011841024379 1101 8 0.31216346534660744 1101 9 0.3521746272838442 1101 10-11 0.3364659218317101 1101 12-13 0.4015596739603353 1101 14-15 0.5126312919556852 1101 16-17 0.46764933919719454 1101 18-19 0.3477399363716316 1101 20-21 0.2868220450818626 1101 22-23 0.284653336998268 1101 24-25 0.40481786984580737 1101 26-27 0.39367569397943214 1101 28-29 0.302580713771718 1101 30-31 0.3861747505296975 1101 32-33 0.49586430779810087 1101 34-35 0.4092964214813435 1101 36-37 0.3609813731869238 1101 38-39 0.3642463878992075 1101 40-41 0.3326345170602707 1101 42-43 0.3222096585758649 1101 44-45 0.481010773208844 1101 46-47 0.4105538662238857 1101 48-49 0.41327754338283285 1101 50-51 0.2596493948834464 1101 52-53 0.4228532910386562 1101 54-55 0.41107768940771905 1101 56-57 0.46005757939300196 1101 58-59 0.5696404523157383 1101 60-61 0.5018730719692073 1101 62-63 0.562757302439941 1101 64-65 0.5381980869559158 1101 66-67 0.4613180990118835 1101 68-69 0.32702291618277 1101 70-71 0.4392908551140806 1101 72-73 0.19835727602028186 1101 74-75 0.42767532465573055 1101 76-77 0.35873968908172316 1101 78-79 0.4160591485420255 1101 80-81 0.5664629258986693 1101 82-83 0.4494494994144915 1101 84-85 0.48678243229170803 1101 86-87 0.38038992784118975 1101 88-89 0.47468458356634 1101 90-91 0.5832245272453598 1101 92-93 0.5015055642145505 1101 94-95 0.5468685396208315 1101 96-97 0.44756426719693465 1101 98-99 0.30753019578000007 1101 100-101 0.2769874173721796 2304 1 0.25517793867085103 2304 2 0.23646395034557344 2304 3 0.24168246954607397 2304 4 0.3014066264686335 2304 5 0.3427875024850806 2304 6 0.3353042215887285 2304 7 0.3424125150132795 2304 8 0.36063809228839006 2304 9 0.3433386141059387 2304 10-11 0.3232362552659751 2304 12-13 0.38858483806961175 2304 14-15 0.5303950054417328 2304 16-17 0.4308470717691151 2304 18-19 0.3796962984889163 2304 20-21 0.3655097571894572 2304 22-23 0.3323846187385371 2304 24-25 0.3909715099928306 2304 26-27 0.43823342534209786 2304 28-29 0.5338397803768871 2304 30-31 0.49398056986071026 2304 32-33 0.4146492830792212 2304 34-35 0.4305309623517459 2304 36-37 0.48263721680242355 2304 38-39 0.4677442862713903 2304 40-41 0.39551524576912556 2304 42-43 0.3892789851922913 2304 44-45 0.4534053891206895 2304 46-47 0.3600456324752912 2304 48-49 0.48071827540189105 2304 50-51 0.3723526505934345 2304 52-53 0.4944498701086175 2304 54-55 0.5748288427493691 2304 56-57 0.5213014523489932 2304 58-59 0.6328367801889101 2304 60-61 0.521666880515177 2304 62-63 0.5587217708904788 2304 64-65 0.5591007677471325 2304 66-67 0.6369152143103207 2304 68-69 0.5842430295755463 2304 70-71 0.6090070158830798 2304 72-73 0.5544458893032029 2304 74-75 0.5689909196975762 2304 76-77 0.526610197843759 2304 78-79 0.5376924711721642 2304 80-81 0.44316976847637335 2304 82-83 0.5936967160115572 2304 84-85 0.5554606733706855 2304 86-87 0.5494838986756783 2304 88-89 0.42230794779846903 2304 90-91 0.5582911244710473 2304 92-93 0.572584695017504 2304 94-95 0.4792214543020279 2304 96-97 0.6223115397529213 2304 98-99 0.5538829794577715 2304 100-101 0.5840884596433789 2202 1 0.17740185764872507 2202 2 0.11623917375099424 2202 3 0.10755326897649908 2202 4 0.2432688486659771 2202 5 0.20832290395923536 2202 6 0.20665771824234724 2202 7 0.40574489691974946 2202 8 0.31192410426144335 2202 9 0.2889191297366551 2202 10-11 0.24247383987833615 2202 12-13 0.3199939572162336 2202 14-15 0.3815489369800389 2202 16-17 0.32537154356064235 2202 18-19 0.31408462296225537 2202 20-21 0.3343541253420881 2202 22-23 0.21505529792140265 2202 24-25 0.36333662391486854 2202 26-27 0.4496336911553058 2202 28-29 0.3461064709224999 2202 30-31 0.29040553679396197 2202 32-33 0.3964654370280769 2202 34-35 0.28723921463389956 2202 36-37 0.24305860562446213 2202 38-39 0.3179627813166448 2202 40-41 0.1449587809835151 2202 42-43 0.3611086216859434 2202 44-45 0.2801981958006081 2202 46-47 0.19270244001442904 2202 48-49 0.33869796177215505 2202 50-51 0.1931360106973088 2202 52-53 0.21029944232631692 2202 54-55 0.46002213564305805 2202 56-57 0.4224146217757294 2202 58-59 0.2719759627453442 2202 60-61 0.19869250694448937 2202 62-63 0.4220609620284037 2202 64-65 0.38990862242880553 2202 66-67 0.27436654696086293 2202 68-69 0.40437285003836365 2202 70-71 0.3336410296864898 2202 72-73 0.2958494677875265 2202 74-75 0.4189057310152364 2202 76-77 0.34907960893718126 2202 78-79 0.417893371483153 2202 80-81 0.48396092921446865 2202 82-83 0.20476794003953813 2202 84-85 0.39005178235330007 2202 86-87 0.2883666623654939 2202 88-89 0.6059986924938663 2202 90-91 0.49678211083419654 2202 92-93 0.315204007810145 2202 94-95 0.28882041855262486 2202 96-97 0.14941487936780717 2202 98-99 0.15368232699747963 2202 100-101 0.2954390087392724 1102 1 0.036627674496941154 1102 2 0.007702781736625752 1102 3 -0.07061100257241293 1102 4 -0.015427001269429752 1102 5 0.04788406328459871 1102 6 0.20148956616081648 1102 7 0.1611694552589995 1102 8 0.1259062376966611 1102 9 0.05900134088649622 1102 10-11 0.026537144802951218 1102 12-13 0.09382089533824711 1102 14-15 0.10513389413303287 1102 16-17 0.05611391310275593 1102 18-19 0.08852093206370881 1102 20-21 0.1187260794618723 1102 22-23 0.14536529867515213 1102 24-25 0.1819143164404906 1102 26-27 0.25644996496027517 1102 28-29 0.1435847745085468 1102 30-31 0.09830357729394024 1102 32-33 0.1530691002716651 1102 34-35 0.12393688917340029 1102 36-37 0.27982515766856153 1102 38-39 0.15296931818170378 1102 40-41 0.1617954321332462 1102 42-43 0.04857980359793146 1102 44-45 0.2718963771745706 1102 46-47 0.17114469077532846 1102 48-49 0.2055342230492201 1102 50-51 0.07932944992005986 1102 52-53 0.13632825131729476 1102 54-55 0.25739257445774655 1102 56-57 0.312061293172853 1102 58-59 0.33578616233415914 1102 60-61 0.2979366737995903 1102 62-63 0.26748060797203976 1102 64-65 0.32740839500308283 1102 66-67 0.38210811856357907 1102 68-69 0.3858883370803028 1102 70-71 0.3895437018737553 1102 72-73 0.373583579826537 1102 74-75 0.6414164590114737 1102 76-77 0.5156354218087031 1102 78-79 0.3765473750855719 1102 80-81 0.35523641064224165 1102 82-83 0.25633912688991245 1102 84-85 0.26710803578093234 1102 86-87 0.19213010322571122 1102 88-89 0.4190503298291084 1102 90-91 0.4156844373343631 1102 92-93 0.467895558749607 1102 94-95 0.4454212227299834 1102 96-97 0.3840796278609453 1102 98-99 0.3326441480667164 1102 100-101 0.2800821842781396 2305 1 0.34960778256524705 2305 2 0.23227586861358063 2305 3 0.29539620920031595 2305 4 0.29460450962943696 2305 5 0.33681626612382587 2305 6 0.4013682870998636 2305 7 0.34204093193984875 2305 8 0.4787611684484858 2305 9 0.3404358989217755 2305 10-11 0.44684813896639497 2305 12-13 0.35309495353053677 2305 14-15 0.36882705810881333 2305 16-17 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-1.0936770408735441 1213 98-99 -1.2084284498103166 1213 100-101 -0.990699668837383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2430.0 3 358.0 4 295.0 5 225.0 6 542.0 7 2766.0 8 11873.0 9 31810.0 10 43075.0 11 32969.0 12 20453.0 13 14813.0 14 11192.0 15 9723.0 16 9399.0 17 9661.0 18 10223.0 19 11060.0 20 12040.0 21 13678.0 22 15377.0 23 16689.0 24 18841.0 25 21315.0 26 25029.0 27 29587.0 28 35745.0 29 43712.0 30 53781.0 31 68397.0 32 90251.0 33 126457.0 34 187595.0 35 303024.0 36 537762.0 37 935764.0 38 458571.0 39 1482.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.22054015454529 25.687157013453703 20.924617266426434 16.167685565574573 2 30.780747383708434 26.50131865954659 29.19387005549398 13.524063901250999 3 36.06440420288993 22.064580459943716 25.8758434102439 15.995171926922454 4 29.709916779633726 21.460160386756865 30.805331360185704 18.0245914734237 5 32.92788938768226 19.863503722158562 30.04487894458327 17.16372794557591 6 29.628616761427523 20.266634439461548 33.356340899874105 16.748407899236824 7 33.50305781209567 21.13507280423432 26.95137737782687 18.41049200584315 8 28.768138077795808 29.506599443795196 24.196559327422527 17.52870315098647 9 30.866119326267576 26.765144767291083 24.263321146805886 18.105414759635458 10-11 26.938482447926525 29.916146388476594 27.73771753314672 15.407653630450158 12-13 27.746051536010253 27.09897113562439 27.53303594392904 17.621941384436315 14-15 25.65300581328368 27.292136965571785 28.594630107640718 18.46022711350382 16-17 26.064913380065153 27.680282726221844 28.95773507650033 17.297068817212672 18-19 25.541300085387526 27.82124818347187 29.342500492320962 17.294951238819646 20-21 24.42913311052867 29.180514119575985 28.749989881006865 17.640362888888475 22-23 26.04768762513971 28.197001533501258 28.04281104755846 17.71249979380057 24-25 23.28529362364249 30.520893105896324 28.279574189366976 17.91423908109421 26-27 22.727872863417897 30.94097762333928 28.213756336307565 18.117393176935256 28-29 23.334536665457104 30.364915010838555 28.102925873235062 18.197622450469275 30-31 24.60985624523709 28.47395398283257 28.59942758608762 18.31676218584272 32-33 24.46246753746762 28.532393480574232 28.694033939508433 18.311105042449714 34-35 22.385998535786033 28.487721348579402 28.38215267508961 20.74412744054495 36-37 21.0521270979958 28.515022093353632 28.78303190541791 21.649818903232653 38-39 20.810395329509852 29.569762476785282 29.76073387893473 19.859108314770133 40-41 21.631364229357956 28.580755962314136 30.937837527719363 18.85004228060854 42-43 22.492139737991266 28.38145664379289 30.319931378665004 18.806472239550843 44-45 21.415104414737254 29.44293691150024 30.271893808703815 18.870064865058694 46-47 22.586376420373693 30.017879184122847 28.34626513746231 19.04947925804115 48-49 20.71371419377209 29.55395162557718 29.85587081195291 19.876463368697816 50-51 20.462417379520492 28.502597618284614 30.52762488879324 20.507360113401653 52-53 23.405558794485895 28.47267170068687 28.361044249159207 19.760725255668028 54-55 23.742469752740384 28.404987469593735 28.73751558116579 19.115027196500094 56-57 24.003659731090547 28.33330188430615 28.506183164644387 19.156855219958917 58-59 22.898958074431984 28.67559350713624 28.360951382731997 20.064497035699773 60-61 21.06438771449543 28.46639439741138 28.228391310182914 22.240826577910276 62-63 22.493144225058607 28.580380445056168 28.419772121942017 20.506703207943207 64-65 20.86721948438555 29.537980516050826 29.806664452569727 19.788135546993896 66-67 20.33523152705674 29.397542738972593 28.874780935684402 21.392444798286263 68-69 20.166640791735485 28.74555216167904 31.458382823036047 19.629424223549428 70-71 20.060957479368646 30.617076821135086 29.784654099498013 19.537311599998255 72-73 19.949767014763967 32.33195163532274 28.128882080747747 19.589399269165547 74-75 19.83841910045819 32.45452420284886 28.20990867437584 19.497148022317116 76-77 19.951854892653802 32.34130286657578 28.073840368703124 19.633001872067297 78-79 19.881006651003492 32.30174593712133 28.19762091228234 19.619626499592833 80-81 19.822627343771675 31.98487839962439 28.293189574625487 19.899304681978442 82-83 19.967284739384848 31.841518072905266 28.099437305000897 20.091759882708992 84-85 19.960358587351415 31.5990449144626 28.33240107107268 20.108195427113305 86-87 20.15658582474074 31.17024198947156 28.366872739212063 20.306299446575636 88-89 20.23265232820693 31.24139857663007 28.27688118234612 20.249067912816876 90-91 20.291080918568973 30.920673038708234 28.322091396185318 20.466154646537476 92-93 20.375707365389033 30.945305387680023 28.163887175590553 20.51510007134039 94-95 20.214964043307617 30.860931507737423 28.37859192894439 20.545512520010572 96-97 20.370305730205203 30.70804632234282 28.256778716006693 20.664869231445287 98-99 20.399716465393254 30.748121770258418 28.219003052922098 20.633158711426233 100-101 20.562560953404784 30.69038226982373 28.088529286318252 20.65852749045323 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1706.0 1 1095.0 2 386.5 3 259.0 4 227.0 5 266.5 6 351.5 7 474.0 8 650.5 9 818.5 10 1013.0 11 1169.5 12 1235.0 13 1246.5 14 1181.0 15 1147.0 16 1151.5 17 1231.0 18 1478.5 19 1957.0 20 2664.5 21 3774.5 22 5472.5 23 7846.0 24 11055.0 25 15103.0 26 20078.0 27 26123.5 28 32940.0 29 40741.0 30 49355.0 31 58149.0 32 67984.5 33 79262.5 34 91329.5 35 103923.5 36 117144.0 37 129953.5 38 140474.5 39 148351.5 40 152098.5 41 151691.0 42 149586.5 43 147115.5 44 143356.5 45 138695.0 46 134347.0 47 128257.5 48 120306.5 49 111940.0 50 102966.0 51 93029.0 52 82995.0 53 72644.5 54 61907.0 55 51936.0 56 43033.0 57 35167.0 58 28420.0 59 22772.5 60 18006.0 61 14090.0 62 10840.0 63 8125.0 64 6035.5 65 4484.0 66 3351.0 67 2514.5 68 1871.0 69 1381.0 70 1038.5 71 791.0 72 605.5 73 464.5 74 377.5 75 301.5 76 251.5 77 209.0 78 156.5 79 135.0 80 118.5 81 99.0 82 74.5 83 68.5 84 69.0 85 56.5 86 42.5 87 38.0 88 36.5 89 33.5 90 29.5 91 22.5 92 17.0 93 12.5 94 10.5 95 10.5 96 6.5 97 2.5 98 2.5 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25761630645961237 2 0.2450617844077808 3 0.2096045822762467 4 0.2128985905373708 5 0.2276905521627961 6 0.2307670315764875 7 0.20761574709971894 8 0.2037623789451964 9 0.18480629366891613 10-11 0.17340156695351472 12-13 0.20081020173003802 14-15 0.19745404236964742 16-17 0.19815324223639544 18-19 0.1906951103244163 20-21 0.19210904783272903 22-23 0.15708690339606035 24-25 0.4614719120537085 26-27 0.3218960808759825 28-29 0.27407080999041633 30-31 0.2772715916026407 32-33 0.31782518387402714 34-35 0.3138475135209717 36-37 0.3067933637542247 38-39 0.33180918120898806 40-41 0.33623744703172564 42-43 0.3717878758121595 44-45 0.3393916153195002 46-47 0.36425205502609725 48-49 0.34120953497304507 50-51 0.3623875220481025 52-53 0.3516353818749992 54-55 0.32834425742488105 56-57 0.36412775282756427 58-59 0.35130908860385013 60-61 0.3179805616221934 62-63 0.2963830546270872 64-65 0.30247386235520346 66-67 0.3108176474317301 68-69 0.3177785705495773 70-71 0.2951555704165739 72-73 0.3018523513625386 74-75 0.3003141116556929 76-77 0.30286230672561903 78-79 0.30458699973026426 80-81 0.32216022304786507 82-83 0.33866133990311886 84-85 0.332881287671335 86-87 0.3368900335740238 88-89 0.33083030139554076 90-91 0.3072439592239068 92-93 0.2922033932014156 94-95 0.30018980945715984 96-97 0.2895619714825896 98-99 0.3073371858728065 100-101 0.32927652391387846 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3217964.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.88674323935093 #Duplication Level Percentage of deduplicated Percentage of total 1 97.77883951523133 91.80136799808224 2 1.6334561184730403 3.0671975037565025 3 0.22161221391847657 0.6241934708060437 4 0.0865367648747135 0.32498620098225234 5 0.06931588490274529 0.3253921344136224 6 0.03336655276490036 0.18796061833322728 7 0.04008983448698419 0.26347327978912927 8 0.021742417181746073 0.16330597914763564 9 0.01041960400724819 0.08804364174758006 >10 0.08975552138064284 1.7856268249039249 >50 0.011797468447117045 0.7307376647560513 >100 0.0029960427756559127 0.4490463116910139 >500 3.8922855970827575E-5 0.02784607220722598 >1k 0.0 0.0 >5k 3.3138699478583386E-5 0.16082229938359024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 5169 0.16062951605425044 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001957759626894521 0.0 0.0 2 0.0022063640239604917 0.0 0.0 3 0.002454968421026463 0.0 0.0 4 0.0026724972684591874 0.0 0.0 5 0.003356159360390607 0.0 0.0 6 0.003946594803422288 0.0 3.107554963324636E-5 7 0.004630256895353708 0.0 3.107554963324636E-5 8 0.005376070086551621 0.0 3.107554963324636E-5 9 0.006090807728116287 0.0 3.107554963324636E-5 10-11 0.008778842771392098 0.0 6.215109926649273E-5 12-13 0.011793171085816994 0.0 1.0876442371636228E-4 14-15 0.014823037175058515 0.0 1.2430219853298545E-4 16-17 0.017495534443517702 0.0 1.2430219853298545E-4 18-19 0.0213799781476735 0.0 1.2430219853298545E-4 20-21 0.02529549740146254 0.0 1.2430219853298545E-4 22-23 0.029381932178234437 0.0 1.2430219853298545E-4 24-25 0.03446278454327022 0.0 1.2430219853298545E-4 26-27 0.04028945009950391 0.0 1.2430219853298545E-4 28-29 0.046085040106104355 0.0 1.2430219853298545E-4 30-31 0.07904066049216213 0.0 1.2430219853298545E-4 32-33 0.08750874776722176 0.0 1.3983997334960865E-4 34-35 0.09569715509558219 3.107554963324636E-5 1.5537774816623182E-4 36-37 0.10475567781367348 3.107554963324636E-5 1.5537774816623182E-4 38-39 0.11547674243714348 3.107554963324636E-5 1.70915522982855E-4 40-41 0.1278914245156254 3.107554963324636E-5 2.0199107261610136E-4 42-43 0.14184434630095302 3.107554963324636E-5 2.1752884743272453E-4 44-45 0.15604587248334661 3.107554963324636E-5 2.1752884743272453E-4 46-47 0.17296650925864926 3.107554963324636E-5 2.486043970659709E-4 48-49 0.19036881705326722 3.107554963324636E-5 2.486043970659709E-4 50-51 0.20848586248944986 3.107554963324636E-5 2.486043970659709E-4 52-53 0.22845190312881064 3.107554963324636E-5 2.486043970659709E-4 54-55 0.24953666355496829 3.107554963324636E-5 2.641421718825941E-4 56-57 0.27298316575325265 3.107554963324636E-5 2.952177215158404E-4 58-59 0.2970045656197521 3.107554963324636E-5 3.262932711490868E-4 60-61 0.3234653961324614 3.107554963324636E-5 3.8844437041557955E-4 62-63 0.352676412787713 3.107554963324636E-5 4.3505769486544907E-4 64-65 0.38497944663147254 4.661332444986954E-5 4.3505769486544907E-4 66-67 0.4211047730801215 6.215109926649273E-5 4.3505769486544907E-4 68-69 0.4582400548918508 6.215109926649273E-5 4.6613324449869546E-4 70-71 0.5001143580226504 6.215109926649273E-5 4.6613324449869546E-4 72-73 0.5459352559568722 6.215109926649273E-5 4.8167101931531864E-4 74-75 0.5967748551568632 6.215109926649273E-5 4.972087941319418E-4 76-77 0.6526797689470734 6.215109926649273E-5 4.972087941319418E-4 78-79 0.7132304774074539 6.215109926649273E-5 5.12746568948565E-4 80-81 0.7807731845353149 9.32266488997391E-5 5.282843437651881E-4 82-83 0.855649721376622 9.32266488997391E-5 5.593598933984345E-4 84-85 0.9371764258394438 9.32266488997391E-5 5.593598933984345E-4 86-87 1.0270624531536088 9.32266488997391E-5 5.593598933984345E-4 88-89 1.1287727271032244 9.32266488997391E-5 5.748976682150577E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 2590 0.0 81.91979 2 GAGCGGC 1600 0.0 80.948204 9 GATCGGG 2845 0.0 76.08473 1 AGAGCGG 1870 0.0 71.043045 8 AAGCGGC 705 0.0 67.29385 9 TCGGGAG 2580 0.0 67.14395 3 GAGAGCG 2270 0.0 64.79796 7 CGGGAGA 2665 0.0 63.93287 4 GTTCGGA 460 0.0 61.911957 2 CGGAAAG 1190 0.0 58.623302 5 AAGCGTC 455 0.0 58.39036 9 GATCCGG 555 0.0 58.16087 1 GGTTCGG 545 0.0 56.61504 1 TTCGGAG 520 0.0 55.675 3 TCGGGAA 965 0.0 55.575687 3 ATCCGGA 530 0.0 55.52607 2 ATTCGGA 670 0.0 53.133392 2 AAAGCGG 965 0.0 52.609188 8 TCGGAGA 740 0.0 52.590717 4 TTCGGAA 660 0.0 52.494358 3 >>END_MODULE