FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_52150223-1.3420000003573b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_52150223-1.3420000003573b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217964
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT51690.16062951605425044Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA25900.081.919792
GAGCGGC16000.080.9482049
GATCGGG28450.076.084731
AGAGCGG18700.071.0430458
AAGCGGC7050.067.293859
TCGGGAG25800.067.143953
GAGAGCG22700.064.797967
CGGGAGA26650.063.932874
GTTCGGA4600.061.9119572
CGGAAAG11900.058.6233025
AAGCGTC4550.058.390369
GATCCGG5550.058.160871
GGTTCGG5450.056.615041
TTCGGAG5200.055.6753
TCGGGAA9650.055.5756873
ATCCGGA5300.055.526072
ATTCGGA6700.053.1333922
AAAGCGG9650.052.6091888
TCGGAGA7400.052.5907174
TTCGGAA6600.052.4943583
Produced by FastQC (version 0.11.2)