FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_50331580-1.342000000357a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_50331580-1.342000000357a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126411
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA15150.083.020592
GAGCGGC10300.078.769539
AGAGCGG12700.067.248438
GATCGGG20550.063.0587271
AAGCGGC4900.062.9385459
TCGGGAG17950.059.7532843
CGGGAGA18500.059.5150834
TTGTGTT299550.057.854471
GATCCGG3000.056.9607431
GAGAGCG17950.056.040077
GTTCGGA3100.055.1179922
TCGGAAA5950.049.452194
GGTTCGG4600.047.467281
GAGCGTC35800.046.9158559
AGAGCGT36850.046.7393268
AAAGCGG6950.046.4234548
TTCGGAG3400.046.063063
GGATCGG7400.045.5429341
TTCGGAA3800.044.961083
TCGGAGA5500.044.8695264