##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_50331580-1.342000000357a5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3126411 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73027666548 33.0 33.0 33.0 27.0 33.0 2 31.596316671096666 33.0 33.0 33.0 27.0 33.0 3 31.537686823645387 33.0 33.0 33.0 27.0 33.0 4 35.171281383029935 37.0 37.0 37.0 33.0 37.0 5 35.15604666181126 37.0 37.0 37.0 33.0 37.0 6 35.30575122720589 37.0 37.0 37.0 33.0 37.0 7 35.16641030242025 37.0 37.0 37.0 33.0 37.0 8 35.21973246639677 37.0 37.0 37.0 33.0 37.0 9 35.002452332722726 37.0 37.0 37.0 33.0 37.0 10-11 34.94716865440916 37.0 37.0 37.0 33.0 37.0 12-13 34.940071219043176 37.0 37.0 37.0 33.0 37.0 14-15 36.62727869112538 40.0 37.0 40.0 33.0 40.0 16-17 36.57680228223353 40.0 37.0 40.0 33.0 40.0 18-19 36.585866189698024 40.0 37.0 40.0 33.0 40.0 20-21 36.565027278883036 40.0 37.0 40.0 33.0 40.0 22-23 36.77010987998699 40.0 37.0 40.0 33.0 40.0 24-25 36.46152409264169 40.0 37.0 40.0 33.0 40.0 26-27 36.35014910067806 40.0 37.0 40.0 33.0 40.0 28-29 36.184290868986835 40.0 37.0 40.0 33.0 40.0 30-31 36.19978451329656 40.0 37.0 40.0 33.0 40.0 32-33 36.043918569887325 40.0 37.0 40.0 30.0 40.0 34-35 36.076770776459014 40.0 37.0 40.0 27.0 40.0 36-37 35.89700154586201 40.0 37.0 40.0 27.0 40.0 38-39 35.835514908308596 40.0 37.0 40.0 27.0 40.0 40-41 35.95126840329054 38.5 37.0 40.0 27.0 40.0 42-43 35.7506340337211 37.0 37.0 40.0 27.0 40.0 44-45 35.545981478442854 37.0 37.0 40.0 27.0 40.0 46-47 35.44679970099901 37.0 37.0 40.0 27.0 40.0 48-49 35.33493900833896 37.0 37.0 40.0 27.0 40.0 50-51 34.26311943631212 37.0 35.0 38.5 24.5 38.5 52-53 34.69245838119173 37.0 35.0 38.5 27.0 40.0 54-55 35.437900199302014 37.0 37.0 40.0 27.0 40.0 56-57 35.55437224984175 37.0 37.0 40.0 27.0 40.0 58-59 35.31239158894976 37.0 37.0 40.0 27.0 40.0 60-61 35.09323326331695 37.0 37.0 40.0 27.0 40.0 62-63 34.86659767381832 37.0 35.0 40.0 27.0 40.0 64-65 34.65505510951695 37.0 33.0 40.0 27.0 40.0 66-67 34.463075871982284 37.0 33.0 40.0 27.0 40.0 68-69 34.30314168546618 37.0 33.0 38.5 27.0 40.0 70-71 33.9810448786164 37.0 33.0 37.0 24.5 40.0 72-73 33.84803485530213 37.0 33.0 37.0 27.0 40.0 74-75 33.68464638846268 37.0 33.0 37.0 27.0 40.0 76-77 33.41776752960503 37.0 33.0 37.0 24.5 38.5 78-79 33.08674787160102 37.0 33.0 37.0 22.0 37.0 80-81 32.75834143367587 37.0 33.0 37.0 22.0 37.0 82-83 32.45364796886909 37.0 33.0 37.0 22.0 37.0 84-85 32.26314630418074 37.0 33.0 37.0 22.0 37.0 86-87 32.07822803847607 37.0 33.0 37.0 22.0 37.0 88-89 31.83201344928738 33.0 33.0 37.0 15.0 37.0 90-91 31.73658357778296 33.0 33.0 37.0 15.0 37.0 92-93 31.567556536872473 33.0 33.0 37.0 15.0 37.0 94-95 31.41857132667458 33.0 33.0 37.0 15.0 37.0 96-97 31.27083243373952 33.0 33.0 37.0 15.0 37.0 98-99 31.11873087063729 33.0 33.0 37.0 15.0 37.0 100-101 29.85912552764176 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.343286336519693 2306 2 0.3752575463027483 2306 3 0.3131911645465131 2306 4 0.4041602381052769 2306 5 0.4909845640994561 2306 6 0.4169076097275237 2306 7 0.5527380817439465 2306 8 0.28787516663648205 2306 9 0.46395850363849434 2306 10-11 0.5017295789249232 2306 12-13 0.4634303613434909 2306 14-15 0.6899670408641398 2306 16-17 0.6661843067788453 2306 18-19 0.7476905772030236 2306 20-21 0.6129598861281949 2306 22-23 0.5391183279524157 2306 24-25 0.72084126781251 2306 26-27 0.6471519920740505 2306 28-29 0.6282885046185811 2306 30-31 0.5935233135977853 2306 32-33 0.5133148818989852 2306 34-35 0.3740282142702682 2306 36-37 0.6292139740103622 2306 38-39 0.4321917530374435 2306 40-41 0.3489152691013402 2306 42-43 0.48616907323817316 2306 44-45 0.6483814710286637 2306 46-47 0.6043140124807493 2306 48-49 0.5873195011295849 2306 50-51 0.5813021464716499 2306 52-53 0.5747636889998304 2306 54-55 0.6041149540529673 2306 56-57 0.5440980452163942 2306 58-59 0.5873434694643365 2306 60-61 0.5919360977727948 2306 62-63 0.6464080422078737 2306 64-65 0.5654299090093886 2306 66-67 0.4906699341086238 2306 68-69 0.5087409153552187 2306 70-71 0.447607570029362 2306 72-73 0.38237536682957085 2306 74-75 0.5320894604515303 2306 76-77 0.3327948944734942 2306 78-79 0.36095522410828806 2306 80-81 0.4572850071842254 2306 82-83 0.444891616572491 2306 84-85 0.2750374733169636 2306 86-87 0.3187840334563887 2306 88-89 0.39252950916866425 2306 90-91 0.2583360621743189 2306 92-93 0.31282189515469483 2306 94-95 0.17055334156930613 2306 96-97 0.18664135224960177 2306 98-99 0.21218866549141424 2306 100-101 0.06853759236390644 2307 1 0.27514627718169393 2307 2 0.28624893700646226 2307 3 0.22612212900173745 2307 4 0.3996721733982369 2307 5 0.3411828731857014 2307 6 0.28503400146919944 2307 7 0.4222850653956982 2307 8 0.26720196015391906 2307 9 0.3387169030855972 2307 10-11 0.3343522939542538 2307 12-13 0.26109997170095056 2307 14-15 0.4673518752476795 2307 16-17 0.4616216505201862 2307 18-19 0.42206976532201423 2307 20-21 0.35249853383558616 2307 22-23 0.27913163451788847 2307 24-25 0.48212315940784123 2307 26-27 0.4813171469240487 2307 28-29 0.5418411190621413 2307 30-31 0.46560089783724834 2307 32-33 0.3965762024724597 2307 34-35 0.44241213060394813 2307 36-37 0.5046565591459498 2307 38-39 0.29418803441972585 2307 40-41 0.31281605266722323 2307 42-43 0.37666148707752 2307 44-45 0.38580647654082867 2307 46-47 0.321284202937413 2307 48-49 0.2673094954175639 2307 50-51 0.3326494039910841 2307 52-53 0.35688205683094765 2307 54-55 0.26979329877811153 2307 56-57 0.31413251721523494 2307 58-59 0.48495302620870717 2307 60-61 0.380361867896724 2307 62-63 0.40296703787163324 2307 64-65 0.314582453567688 2307 66-67 0.3577328965988116 2307 68-69 0.2174465617487158 2307 70-71 0.17273447881878923 2307 72-73 0.1721384016548555 2307 74-75 0.20405377126298418 2307 76-77 0.2645154192060488 2307 78-79 0.24154781756048038 2307 80-81 0.14400339151961816 2307 82-83 0.3359914970454909 2307 84-85 0.4312091022236757 2307 86-87 0.3522941429647801 2307 88-89 0.35914318166468817 2307 90-91 0.3568821716156627 2307 92-93 0.43487224090619847 2307 94-95 0.21402838595675888 2307 96-97 0.4173673014706125 2307 98-99 0.3787006046525576 2307 100-101 0.18650297079515 2201 1 0.3563162229133887 2201 2 0.31326622544164096 2201 3 0.26657867418758485 2201 4 0.3136347577283516 2201 5 0.4036985462319507 2201 6 0.35245183818710046 2201 7 0.5433193281964677 2201 8 0.30894704277369556 2201 9 0.46419013778322693 2201 10-11 0.3960733587718792 2201 12-13 0.40850512390296245 2201 14-15 0.6232475578864509 2201 16-17 0.5567415204126007 2201 18-19 0.5186534673016254 2201 20-21 0.47244816096925035 2201 22-23 0.5680503951846916 2201 24-25 0.6041203254776164 2201 26-27 0.5594635642178432 2201 28-29 0.6236848536173341 2201 30-31 0.507524014089249 2201 32-33 0.6346217766476556 2201 34-35 0.4935525197093824 2201 36-37 0.5701525456287868 2201 38-39 0.5105551988095911 2201 40-41 0.4957028512755741 2201 42-43 0.5773553008805123 2201 44-45 0.6470932001620255 2201 46-47 0.6141452673168075 2201 48-49 0.5300974838383752 2201 50-51 0.3654973699644444 2201 52-53 0.6154316274807812 2201 54-55 0.7170216955380795 2201 56-57 0.5608773928899282 2201 58-59 0.6688925057296657 2201 60-61 0.7014243726138503 2201 62-63 0.7248104559226007 2201 64-65 0.7437354151952036 2201 66-67 0.7305584516376769 2201 68-69 0.5918963313092789 2201 70-71 0.6406184288890344 2201 72-73 0.5753952302337311 2201 74-75 0.5085341930413989 2201 76-77 0.5727680236707045 2201 78-79 0.6123745266224816 2201 80-81 0.7300444591614408 2201 82-83 0.6383683829846731 2201 84-85 0.6721083881414245 2201 86-87 0.8403190226323076 2201 88-89 0.765615267208485 2201 90-91 0.6128501024327058 2201 92-93 0.6948853393122327 2201 94-95 0.5571226287447253 2201 96-97 0.449121806178006 2201 98-99 0.5881061975258568 2201 100-101 0.5290424181787579 1101 1 0.26705716698381465 1101 2 0.1839876857441034 1101 3 0.2054843991520201 1101 4 0.28407738882453515 1101 5 0.28355171261335954 1101 6 0.332133820489112 1101 7 0.39285580261459785 1101 8 0.2663045588680575 1101 9 0.336592136809891 1101 10-11 0.24554581266718856 1101 12-13 0.2778181327032385 1101 14-15 0.43856754152438526 1101 16-17 0.42531064473082836 1101 18-19 0.3513525442163399 1101 20-21 0.41853501982213004 1101 22-23 0.34787907229973314 1101 24-25 0.5038882652462036 1101 26-27 0.41255346092950873 1101 28-29 0.49170038454293774 1101 30-31 0.5596268049874595 1101 32-33 0.5664200196525613 1101 34-35 0.3913739947988901 1101 36-37 0.5044778288062872 1101 38-39 0.3172304997485398 1101 40-41 0.3978891946216052 1101 42-43 0.41566594658697653 1101 44-45 0.5014050107020651 1101 46-47 0.3220616174218165 1101 48-49 0.3284152737819426 1101 50-51 0.37553343674748163 1101 52-53 0.4770050932969454 1101 54-55 0.46295455104036165 1101 56-57 0.49952087449593563 1101 58-59 0.5112938787149375 1101 60-61 0.5922948052736103 1101 62-63 0.5727571109635932 1101 64-65 0.5613653074537623 1101 66-67 0.40655752588958904 1101 68-69 0.3080528450575528 1101 70-71 0.3977472274164384 1101 72-73 0.3061961553019543 1101 74-75 0.5178530033474544 1101 76-77 0.49923584995866577 1101 78-79 0.32112406417335393 1101 80-81 0.5142526640996365 1101 82-83 0.4458219156994403 1101 84-85 0.577230539865532 1101 86-87 0.41551218897971864 1101 88-89 0.4235136009185787 1101 90-91 0.4495107324739642 1101 92-93 0.4829894480134165 1101 94-95 0.5789019998592302 1101 96-97 0.37949771712489166 1101 98-99 0.3145951562518796 1101 100-101 0.3411222126785418 2304 1 0.19458550250327278 2304 2 0.14857249050648846 2304 3 0.13030866848239597 2304 4 0.24466663309857495 2304 5 0.2539616947880461 2304 6 0.3227032214913663 2304 7 0.3441355254814482 2304 8 0.2834639440629587 2304 9 0.23171203725720346 2304 10-11 0.24909069545735463 2304 12-13 0.23234340765026928 2304 14-15 0.3404139747803683 2304 16-17 0.31483743314772994 2304 18-19 0.34088269589965137 2304 20-21 0.3383984712061121 2304 22-23 0.3530585025623978 2304 24-25 0.3288134325687011 2304 26-27 0.40252083724538323 2304 28-29 0.35290846983536994 2304 30-31 0.3703354452188492 2304 32-33 0.30988014812934495 2304 34-35 0.4157307700990529 2304 36-37 0.405281649476926 2304 38-39 0.38483215447561037 2304 40-41 0.2874294361041265 2304 42-43 0.46772594725963046 2304 44-45 0.40022450419414923 2304 46-47 0.475123871482964 2304 48-49 0.4568046055009063 2304 50-51 0.4108073747961143 2304 52-53 0.3937407069248948 2304 54-55 0.512475285577743 2304 56-57 0.40168009152750983 2304 58-59 0.5797800725063453 2304 60-61 0.5512427755423346 2304 62-63 0.44526240810817797 2304 64-65 0.43412284957867797 2304 66-67 0.5014386344222004 2304 68-69 0.4406664393803581 2304 70-71 0.4667244581028882 2304 72-73 0.3822530617695179 2304 74-75 0.5461178942094591 2304 76-77 0.5845652819029681 2304 78-79 0.5269021558559928 2304 80-81 0.5282303992760333 2304 82-83 0.5695295805835769 2304 84-85 0.5114013265335444 2304 86-87 0.4858683588919419 2304 88-89 0.4298735376891578 2304 90-91 0.5759998186744859 2304 92-93 0.6048944021048293 2304 94-95 0.5581640992101207 2304 96-97 0.63058573380669 2304 98-99 0.5517289806221086 2304 100-101 0.4966766471452999 2202 1 0.24220215638398201 2202 2 0.2655130392459917 2202 3 0.308769614418118 2202 4 0.3574228914961992 2202 5 0.3990736141133482 2202 6 0.4035741170898035 2202 7 0.4091770939088235 2202 8 0.26602102288759966 2202 9 0.3388688593623428 2202 10-11 0.4091713126345553 2202 12-13 0.40115849020253336 2202 14-15 0.5014128049747342 2202 16-17 0.4636121383360816 2202 18-19 0.39156391542359614 2202 20-21 0.4526582830993675 2202 22-23 0.2926875284975736 2202 24-25 0.3015905986629761 2202 26-27 0.45958565673205953 2202 28-29 0.4515215842881304 2202 30-31 0.5065480259751354 2202 32-33 0.5312553293679372 2202 34-35 0.47840046538063064 2202 36-37 0.4415164259039983 2202 38-39 0.5247123978902124 2202 40-41 0.3999824946988255 2202 42-43 0.44004056807941794 2202 44-45 0.5178420521539877 2202 46-47 0.5392515409813043 2202 48-49 0.5282988618240196 2202 50-51 0.522480922120721 2202 52-53 0.47140892353713326 2202 54-55 0.6059282504504395 2202 56-57 0.5621730479971134 2202 58-59 0.6627624328877673 2202 60-61 0.5421220429726574 2202 62-63 0.58241952407149 2202 64-65 0.5668967894853054 2202 66-67 0.6229863791857397 2202 68-69 0.6694025178492424 2202 70-71 0.7206446199624779 2202 72-73 0.6237774104203666 2202 74-75 0.5512052044755578 2202 76-77 0.5050530739714247 2202 78-79 0.5761591635919672 2202 80-81 0.5977208554502127 2202 82-83 0.7817755886452957 2202 84-85 0.6676345273806064 2202 86-87 0.7790779841852498 2202 88-89 0.8038764049946927 2202 90-91 0.8322236410691026 2202 92-93 0.7058614455977157 2202 94-95 0.6592242394742591 2202 96-97 0.6450243615265236 2202 98-99 0.7023391739995439 2202 100-101 0.632257995529173 1102 1 0.004992601351283099 1102 2 -0.05225060151928318 1102 3 -0.1187190029014431 1102 4 0.002913008475943002 1102 5 0.035718789176677035 1102 6 0.021205846418212104 1102 7 0.09780083234193171 1102 8 0.03490579066264843 1102 9 0.002705893083536637 1102 10-11 -0.011819458373011571 1102 12-13 0.017257683660382384 1102 14-15 0.07870528607091387 1102 16-17 0.054908099086240725 1102 18-19 0.0619030025829872 1102 20-21 0.031616605864797975 1102 22-23 0.07531992255191966 1102 24-25 0.057709863718891086 1102 26-27 0.1590595135296624 1102 28-29 0.04561041571434998 1102 30-31 0.11360923052585292 1102 32-33 0.07339739127808542 1102 34-35 0.08769084579859765 1102 36-37 0.04125182170682962 1102 38-39 0.04455470758807678 1102 40-41 0.11583655694361994 1102 42-43 -7.434094530864854E-4 1102 44-45 0.14057690535774015 1102 46-47 0.19044453772691128 1102 48-49 0.22241464289863444 1102 50-51 0.1204326767207391 1102 52-53 0.15654597608222076 1102 54-55 0.3074851756325856 1102 56-57 0.31049861177005056 1102 58-59 0.22975136141445773 1102 60-61 0.2018196852270151 1102 62-63 0.2961277115477685 1102 64-65 0.34053098202598875 1102 66-67 0.36961953629256783 1102 68-69 0.3398700425613015 1102 70-71 0.279454895159013 1102 72-73 0.3372677288613559 1102 74-75 0.5336200780850717 1102 76-77 0.4185686832510527 1102 78-79 0.3551778165470907 1102 80-81 0.2862783015947912 1102 82-83 0.2454899666072592 1102 84-85 0.26549925457010914 1102 86-87 0.2531210372702901 1102 88-89 0.4050380766806576 1102 90-91 0.3552845826143276 1102 92-93 0.3122568423765948 1102 94-95 0.18191874032757838 1102 96-97 0.22297399573493237 1102 98-99 0.17559309041332583 1102 100-101 0.12430869067864592 2305 1 0.18544502005962116 2305 2 0.03605504314741026 2305 3 0.01696605332982415 2305 4 -0.0682143521805969 2305 5 0.03814427942312193 2305 6 0.18338135683013235 2305 7 0.10228049765357383 2305 8 0.14136667481636778 2305 9 0.04426007242967245 2305 10-11 0.1167470712585299 2305 12-13 0.18354582514228213 2305 14-15 0.13045711414477523 2305 16-17 0.17305166738006506 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-0.9556303665804577 1213 54-55 -1.1079037657857285 1213 56-57 -1.0665193686725516 1213 58-59 -0.9851329317231503 1213 60-61 -1.0043763218278556 1213 62-63 -1.0685538124674494 1213 64-65 -0.9111846026847772 1213 66-67 -0.8766815970777415 1213 68-69 -0.942486120545226 1213 70-71 -0.9614917734703639 1213 72-73 -0.8134161664265491 1213 74-75 -0.821934472799505 1213 76-77 -0.8970082028309605 1213 78-79 -1.0420423932186011 1213 80-81 -1.1397848507594759 1213 82-83 -1.163595256170474 1213 84-85 -1.2155648420325065 1213 86-87 -1.085559819764427 1213 88-89 -1.0006114116072204 1213 90-91 -1.0819590227260605 1213 92-93 -1.0770442953185189 1213 94-95 -1.141097685428953 1213 96-97 -1.0744067282400565 1213 98-99 -1.2378228204675743 1213 100-101 -1.1223854390119037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2361.0 3 314.0 4 294.0 5 237.0 6 553.0 7 2987.0 8 9786.0 9 21610.0 10 30495.0 11 24552.0 12 16245.0 13 11851.0 14 9345.0 15 8420.0 16 7956.0 17 8209.0 18 9085.0 19 9978.0 20 11217.0 21 12639.0 22 14266.0 23 15560.0 24 17212.0 25 19892.0 26 23362.0 27 27388.0 28 33055.0 29 40218.0 30 49741.0 31 63927.0 32 85215.0 33 119032.0 34 178570.0 35 291233.0 36 538444.0 37 960709.0 38 447503.0 39 2950.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.54182191569525 24.238955071821014 24.3600757701843 15.85914724229944 2 29.986391975241343 23.797850932489574 33.021071276037695 13.194685816231386 3 37.95630755679834 20.58885466719105 25.802049006796153 15.652788769214446 4 29.000214759421368 20.248928606916536 34.439910634437794 16.3109459992243 5 32.25379145498838 18.628020172364657 33.05566571143619 16.06252266121077 6 26.33610827297917 19.373934631881554 38.56513168166344 15.724825413475838 7 33.34572659900371 19.53487432908095 29.94342260544479 17.175976466470555 8 28.642392596115755 29.229618870212803 25.89490950907858 16.233079024592865 9 32.27788434239307 26.7467428719381 23.63323670952679 17.342136076142037 10-11 27.693974787599085 28.948715389580443 27.34968288650895 16.007626936311524 12-13 28.72932877955009 26.20975424729812 26.872262085363722 18.188654887788065 14-15 26.80299474371905 26.64789583711907 28.090128184543182 18.458981234618705 16-17 26.027661992450575 27.53794742580534 28.50819392623255 17.926196655511532 18-19 25.08295621350812 28.143230918789037 28.898918000052237 17.87489486765061 20-21 24.279404542442894 28.764211658066163 28.76373095932247 18.19265284016847 22-23 25.10816602858681 28.396339614499492 28.13779193134527 18.35770242556843 24-25 23.72328542801799 29.33013214771855 28.47331209920912 18.47327032505434 26-27 23.193509994509622 29.76384956685057 28.249727807529357 18.792912631110454 28-29 23.228226874970133 29.75946678178973 28.10650251794281 18.905803825297333 30-31 23.746449780531886 28.644670140308843 28.52334925820259 19.085530820956677 32-33 22.60307742167865 29.542943276833984 28.837002221307706 19.01697708017966 34-35 23.713819995604094 28.859101534235936 28.714582059883814 18.712496410276156 36-37 22.77880973652139 29.22417860353015 29.026282088199668 18.970729571748794 38-39 22.533549015797654 30.20360555141118 28.29346465124123 18.969380781549937 40-41 23.916544882656485 28.46899085171674 28.190397391642453 19.42406687398432 42-43 23.72823809867803 28.63273054170981 28.25644730188543 19.382584057726728 44-45 22.438506717454178 29.06241931055245 29.202065714047755 19.29700825794562 46-47 22.73411890780225 29.107904954788722 28.535233591198107 19.622742546210915 48-49 21.654318986594216 29.52772831840726 28.863079968513617 19.95487272648491 50-51 21.24992034383853 28.629668432933364 29.583760640819172 20.53665058240893 52-53 22.896611717349398 28.67417050391696 28.274529378437673 20.15468840029596 54-55 23.09658166451546 28.614832491356125 28.617849103905613 19.670736740222804 56-57 23.413264811465343 28.405258180151133 28.211063662028984 19.970413346354544 58-59 22.80990565628921 28.73093569149276 28.470448054784896 19.98871059743313 60-61 21.58973894923159 28.555879330001886 28.503831544872604 21.35055017589392 62-63 22.426233400008662 28.833577417435148 28.15757941288283 20.582609769673365 64-65 21.469013377548183 29.177469997046746 29.173395437744826 20.180121187660248 66-67 21.286864638519628 28.88043992509196 28.54796451863019 21.28473091775822 68-69 21.121354378916767 28.745431441947627 29.976912537182205 20.1563016419534 70-71 20.865740217943653 29.739911893481707 29.334379379019715 20.059968509554924 72-73 20.75963658255937 31.10128407559855 28.006104761075186 20.13297458076689 74-75 20.747417057749338 30.995563665949305 28.297638617793847 19.95938065850751 76-77 21.111536392170024 30.590366753045423 28.099761525339495 20.19833532944506 78-79 20.99849380992616 30.67648291703891 28.412353100747893 19.912670172287033 80-81 20.594507354808734 30.56700834917881 28.565416394374793 20.273067901637667 82-83 20.575598886115714 30.624259511352513 28.255097025175367 20.5450445773564 84-85 20.49938701892218 30.325505789622326 28.727277978895476 20.447829212560016 86-87 20.748786793561298 29.909813279902664 28.731565608788845 20.609834317747193 88-89 20.903884180462327 30.442936913538272 28.44050461723907 20.21267428876033 90-91 21.038629192732714 29.804922544615213 28.466065102179762 20.69038316047231 92-93 20.840041325024913 30.127922477563885 28.213600527004743 20.81843567040646 94-95 20.52855211939624 30.209733941748805 28.62084044365179 20.640873495203163 96-97 20.91180081784273 30.141731071046777 28.222583462382683 20.723884648727804 98-99 21.016523512303962 30.064581892525222 28.064623923615073 20.85427067155575 100-101 21.099826014066174 29.73023756148196 28.062071403390792 21.107865021061077 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1677.0 1 1081.5 2 383.5 3 267.5 4 274.5 5 295.0 6 345.0 7 468.0 8 606.5 9 790.0 10 957.5 11 1108.5 12 1231.5 13 1207.0 14 1136.5 15 1075.5 16 1042.0 17 1036.0 18 1155.0 19 1429.0 20 1851.0 21 2440.5 22 3320.0 23 4649.0 24 6436.5 25 8797.5 26 11816.5 27 15484.5 28 19931.0 29 24992.5 30 30747.5 31 37852.0 32 46599.0 33 57060.5 34 69844.0 35 86660.5 36 107381.0 37 130667.0 38 154245.0 39 169973.0 40 175630.5 41 170790.5 42 161304.0 43 157514.0 44 153400.5 45 149871.5 46 149866.5 47 142425.5 48 126161.5 49 110882.5 50 99897.5 51 89936.0 52 79071.5 53 68097.5 54 57185.0 55 46914.5 56 37973.0 57 30383.5 58 24368.5 59 19647.0 60 15744.0 61 12177.5 62 9250.5 63 7118.0 64 5432.5 65 4120.0 66 3164.0 67 2374.0 68 1771.5 69 1345.5 70 1030.5 71 823.5 72 634.0 73 482.5 74 377.5 75 312.0 76 268.0 77 206.0 78 152.0 79 127.0 80 110.0 81 94.5 82 75.0 83 67.0 84 69.5 85 58.5 86 44.0 87 36.5 88 25.5 89 19.0 90 19.5 91 15.0 92 13.0 93 13.5 94 13.5 95 9.5 96 5.5 97 5.5 98 4.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2569719720151957 2 0.24510533004137972 3 0.20976128858297902 4 0.21241608988709415 5 0.22690554760714443 6 0.2297842478164259 7 0.20589103607938944 8 0.20211674024944257 9 0.18286143440513739 10-11 0.17168248192576088 12-13 0.19899814835605428 14-15 0.19651926762028407 16-17 0.19744684879883034 18-19 0.1899782210336389 20-21 0.19042601884397156 22-23 0.15789670647909057 24-25 0.46183947024239613 26-27 0.3214548567031014 28-29 0.2746919710812174 30-31 0.2765471334383099 32-33 0.31996752826163927 34-35 0.31588936963182385 36-37 0.3080369151720615 38-39 0.3329856503191679 40-41 0.33842319515892183 42-43 0.3753825072903083 44-45 0.34174969317853604 46-47 0.3689694029351867 48-49 0.34475633561934116 50-51 0.3665704860941188 52-53 0.3555514614041468 54-55 0.3305067695833977 56-57 0.36761001672524823 58-59 0.3550396924780523 60-61 0.32126294335581596 62-63 0.2979774572185167 64-65 0.3041666626684719 66-67 0.3131226188751255 68-69 0.31988756436693705 70-71 0.2966660493453996 72-73 0.3038468070896629 74-75 0.30139991191177357 76-77 0.3044385399104596 78-79 0.30726926178291974 80-81 0.32358189630218165 82-83 0.3405662275369425 84-85 0.3355604877285808 86-87 0.3389669496428972 88-89 0.33346543368738146 90-91 0.3086286479928583 92-93 0.2933555441047258 94-95 0.3026473486691289 96-97 0.289901103853588 98-99 0.3093163374872977 100-101 0.3311944590778372 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3126411.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.41155895648947 #Duplication Level Percentage of deduplicated Percentage of total 1 94.82731883427982 76.25212539128516 2 3.204556855471473 5.153668250263337 3 0.6944733935699068 1.6753106469227965 4 0.331196763133368 1.065281921795892 5 0.2439591309012235 0.9808567018718835 6 0.1272289288505799 0.6138405907943645 7 0.06551792026099607 0.3687878675441563 8 0.0650571957800552 0.418508042720943 9 0.057904469579312445 0.419056980247902 >10 0.3115879292094159 4.6195028852381395 >50 0.03931531232497233 2.2524416280900117 >100 0.029933810825520272 4.833489200515499 >500 0.0015373647136987904 0.8627479459737211 >1k 4.120910996819624E-4 0.4843819467362286 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012154511994744133 0.0 0.0 2 0.001407364546759847 0.0 0.0 3 0.0017272201255689032 0.0 0.0 4 0.0017911912413307144 0.0 0.0 5 0.002015090146497054 0.0 0.0 6 0.0023349457253061096 0.0 0.0 7 0.003070613556566939 0.0 3.1985557880905615E-5 8 0.0037742958299468625 0.0 3.1985557880905615E-5 9 0.004030180292994108 0.0 3.1985557880905615E-5 10-11 0.005597472629158483 0.0 4.797833682135842E-5 12-13 0.006573032144526104 0.0 6.397111576181123E-5 14-15 0.007772490565060064 0.0 7.996389470226404E-5 16-17 0.008971948985594023 0.0 1.1194945258316965E-4 18-19 0.010491262984937041 0.0 1.5992778940452808E-4 20-21 0.012058555321101415 0.0 1.5992778940452808E-4 22-23 0.013561876541503981 0.0 1.5992778940452808E-4 24-25 0.015161154435549261 0.0 1.5992778940452808E-4 26-27 0.016904367340058617 0.0 1.5992778940452808E-4 28-29 0.01914335639172201 0.0 1.5992778940452808E-4 30-31 0.033248987417201385 0.0 1.7592056834498087E-4 32-33 0.03612768762648289 0.0 2.079061262258865E-4 34-35 0.03854259724649127 0.0 2.558844630472449E-4 36-37 0.04121339132954688 0.0 2.558844630472449E-4 38-39 0.043852199854721594 0.0 2.558844630472449E-4 40-41 0.04745057511632347 0.0 2.558844630472449E-4 42-43 0.051592704861900755 0.0 2.558844630472449E-4 44-45 0.05616663963887025 0.0 2.8787002092815056E-4 46-47 0.0612363505629938 0.0 3.1985557880905615E-4 48-49 0.06656194595016458 0.0 3.1985557880905615E-4 50-51 0.07331089866303567 0.0 3.3584835774950897E-4 52-53 0.08010782971272812 0.0 3.5184113668996174E-4 54-55 0.0883121253091804 0.0 3.838266945708674E-4 56-57 0.09667634869503722 0.0 4.1581225245177297E-4 58-59 0.10671981386964158 0.0 4.1581225245177297E-4 60-61 0.11810667247524398 0.0 4.1581225245177297E-4 62-63 0.1307409678382017 0.0 4.1581225245177297E-4 64-65 0.14583815115798915 0.0 4.477978103326786E-4 66-67 0.16315833075049951 0.0 4.477978103326786E-4 68-69 0.18196583878447203 0.0 4.797833682135842E-4 70-71 0.20227666803884709 0.0 4.957761471540371E-4 72-73 0.22580204586025318 0.0 5.117689260944898E-4 74-75 0.2524300227961071 0.0 5.117689260944898E-4 76-77 0.2816808154781953 0.0 5.117689260944898E-4 78-79 0.3159373479686452 0.0 5.117689260944898E-4 80-81 0.35462388022560054 0.0 5.437544839753954E-4 82-83 0.3957253221025643 0.0 5.75740041856301E-4 84-85 0.4439595433869699 0.0 5.75740041856301E-4 86-87 0.49812708565828356 0.0 5.917328207967539E-4 88-89 0.5584518478216716 0.0 6.077255997372067E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1515 0.0 83.02059 2 GAGCGGC 1030 0.0 78.76953 9 AGAGCGG 1270 0.0 67.24843 8 GATCGGG 2055 0.0 63.058727 1 AAGCGGC 490 0.0 62.938545 9 TCGGGAG 1795 0.0 59.753284 3 CGGGAGA 1850 0.0 59.515083 4 TTGTGTT 29955 0.0 57.85447 1 GATCCGG 300 0.0 56.960743 1 GAGAGCG 1795 0.0 56.04007 7 GTTCGGA 310 0.0 55.117992 2 TCGGAAA 595 0.0 49.45219 4 GGTTCGG 460 0.0 47.46728 1 GAGCGTC 3580 0.0 46.915855 9 AGAGCGT 3685 0.0 46.739326 8 AAAGCGG 695 0.0 46.423454 8 TTCGGAG 340 0.0 46.06306 3 GGATCGG 740 0.0 45.542934 1 TTCGGAA 380 0.0 44.96108 3 TCGGAGA 550 0.0 44.869526 4 >>END_MODULE