##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l04n02_50330665-2.34200000035755.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2522373 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.018138078706045 33.0 33.0 33.0 33.0 33.0 2 31.983019561341642 33.0 33.0 33.0 33.0 33.0 3 31.960763931424893 33.0 33.0 33.0 33.0 33.0 4 35.690973143147346 37.0 37.0 37.0 33.0 37.0 5 35.665098698725366 37.0 37.0 37.0 33.0 37.0 6 35.69317107342966 37.0 37.0 37.0 33.0 37.0 7 35.66373807521727 37.0 37.0 37.0 33.0 37.0 8 35.63245998906585 37.0 37.0 37.0 33.0 37.0 9 35.53516985790761 37.0 37.0 37.0 33.0 37.0 10-11 35.46893124054214 37.0 37.0 37.0 33.0 37.0 12-13 35.477910285275016 37.0 37.0 37.0 33.0 37.0 14-15 37.206087878358986 40.0 37.0 40.0 33.0 40.0 16-17 37.18080831026973 40.0 37.0 40.0 33.0 40.0 18-19 37.22090586919539 40.0 37.0 40.0 33.0 40.0 20-21 37.222279179169774 40.0 37.0 40.0 33.0 40.0 22-23 37.25546756962591 40.0 37.0 40.0 33.0 40.0 24-25 37.06193235496892 40.0 37.0 40.0 33.0 40.0 26-27 36.98333295670386 40.0 37.0 40.0 33.0 40.0 28-29 36.90999427919661 40.0 37.0 40.0 33.0 40.0 30-31 36.94547317149367 40.0 37.0 40.0 33.0 40.0 32-33 36.830131982859 40.0 37.0 40.0 33.0 40.0 34-35 36.7706845894719 40.0 37.0 40.0 33.0 40.0 36-37 36.674056929724514 40.0 37.0 40.0 33.0 40.0 38-39 36.63220566506223 40.0 37.0 40.0 33.0 40.0 40-41 36.59823923741651 40.0 37.0 40.0 33.0 40.0 42-43 36.45504828191548 40.0 37.0 40.0 33.0 40.0 44-45 36.35794725839517 40.0 37.0 40.0 33.0 40.0 46-47 36.29989081709961 38.5 37.0 40.0 33.0 40.0 48-49 36.19985446244469 37.0 37.0 40.0 33.0 40.0 50-51 35.084884749400665 37.0 35.0 38.5 30.0 38.5 52-53 35.50136161463828 37.0 37.0 38.5 30.0 40.0 54-55 36.28944807132014 37.0 37.0 40.0 33.0 40.0 56-57 36.34177241034534 37.0 37.0 40.0 33.0 40.0 58-59 36.16891633394427 37.0 37.0 40.0 33.0 40.0 60-61 35.92471176943299 37.0 37.0 40.0 30.0 40.0 62-63 35.74972377201944 37.0 37.0 40.0 27.0 40.0 64-65 35.52644573185647 37.0 37.0 40.0 27.0 40.0 66-67 35.29268589538502 37.0 37.0 40.0 27.0 40.0 68-69 35.06912141860066 37.0 33.0 38.5 27.0 40.0 70-71 34.785210395131884 37.0 33.0 37.0 27.0 40.0 72-73 34.55310812476981 37.0 33.0 37.0 27.0 40.0 74-75 34.32868850086803 37.0 33.0 37.0 27.0 40.0 76-77 34.09364594372046 37.0 33.0 37.0 27.0 37.0 78-79 33.834171036559624 37.0 33.0 37.0 27.0 37.0 80-81 33.597995419392774 37.0 33.0 37.0 27.0 37.0 82-83 33.37751216810519 37.0 33.0 37.0 27.0 37.0 84-85 33.21688108776934 37.0 33.0 37.0 27.0 37.0 86-87 33.049687932752214 37.0 33.0 37.0 27.0 37.0 88-89 32.83466243890178 37.0 33.0 37.0 27.0 37.0 90-91 32.76810388471491 37.0 33.0 37.0 27.0 37.0 92-93 32.60919261346359 35.0 33.0 37.0 27.0 37.0 94-95 32.47958113252877 33.0 33.0 37.0 24.5 37.0 96-97 32.33853795612306 33.0 33.0 37.0 22.0 37.0 98-99 32.20515007098474 33.0 33.0 37.0 22.0 37.0 100-101 31.016343340180065 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.28019913704711996 2306 2 0.2582613217678613 2306 3 0.1811976466476075 2306 4 0.23979614876 2306 5 0.36069572272815265 2306 6 0.3412477134019767 2306 7 0.3397983846083008 2306 8 0.2800771049199611 2306 9 0.4222618929294697 2306 10-11 0.36051195762868815 2306 12-13 0.36244797773766635 2306 14-15 0.4659384999644729 2306 16-17 0.4293355756703292 2306 18-19 0.44328689957480805 2306 20-21 0.4938256503445331 2306 22-23 0.4230989759615724 2306 24-25 0.5565285495064245 2306 26-27 0.5575842824237185 2306 28-29 0.4284251813042772 2306 30-31 0.3659804869094714 2306 32-33 0.42906023491003964 2306 34-35 0.37002046657190846 2306 36-37 0.35337787755439365 2306 38-39 0.39515770834191954 2306 40-41 0.3508497250295619 2306 42-43 0.40246889809315434 2306 44-45 0.4351592673705582 2306 46-47 0.5392404677957927 2306 48-49 0.4347156927789797 2306 50-51 0.2850941908539255 2306 52-53 0.42510699534276597 2306 54-55 0.5286348106897663 2306 56-57 0.49618164094384554 2306 58-59 0.5551518103414068 2306 60-61 0.5875587099025879 2306 62-63 0.49694468830989535 2306 64-65 0.5360166662768293 2306 66-67 0.5357719097919684 2306 68-69 0.5193994587712254 2306 70-71 0.6039115840345843 2306 72-73 0.49539766311929156 2306 74-75 0.5540817609305222 2306 76-77 0.5208883016809622 2306 78-79 0.39844889723108423 2306 80-81 0.4182469609501638 2306 82-83 0.5404231329463371 2306 84-85 0.5062502456554085 2306 86-87 0.41548853674206754 2306 88-89 0.5627451081459682 2306 90-91 0.4809204836147032 2306 92-93 0.48654080295995783 2306 94-95 0.2876363651787486 2306 96-97 0.3262082502621624 2306 98-99 0.35909760049161576 2306 100-101 0.29439518451466284 2307 1 0.29954066998676865 2307 2 0.3256978693294883 2307 3 0.24735748682698144 2307 4 0.34252859043503037 2307 5 0.34850114394110676 2307 6 0.36823183779961255 2307 7 0.38709250366120074 2307 8 0.38480051486116906 2307 9 0.35959622785753 2307 10-11 0.4396644205522975 2307 12-13 0.2375619064223855 2307 14-15 0.516604717977529 2307 16-17 0.5204277375940904 2307 18-19 0.601420592917357 2307 20-21 0.38053178875145477 2307 22-23 0.38002836570576193 2307 24-25 0.48622735411071716 2307 26-27 0.48548711469896944 2307 28-29 0.4945096574550263 2307 30-31 0.4448940933891947 2307 32-33 0.35247435848206266 2307 34-35 0.3083726911550855 2307 36-37 0.4561845969297167 2307 38-39 0.3492968637433549 2307 40-41 0.3876929580730675 2307 42-43 0.26293952812013544 2307 44-45 0.3364173170635638 2307 46-47 0.16896355183850176 2307 48-49 0.12195185078326176 2307 50-51 0.20423130395808897 2307 52-53 0.22754392855096484 2307 54-55 0.11929366339668235 2307 56-57 0.18630811128925728 2307 58-59 0.19821514219733416 2307 60-61 0.1976654199354826 2307 62-63 0.08450533258950088 2307 64-65 0.14895231109960605 2307 66-67 0.2611906078926296 2307 68-69 0.2630752625455699 2307 70-71 0.25264650036557157 2307 72-73 0.023441636604189853 2307 74-75 0.12835484432143573 2307 76-77 0.22045482951486406 2307 78-79 0.28444172529138 2307 80-81 0.20198926974551057 2307 82-83 0.27386355109583604 2307 84-85 0.27825246828319905 2307 86-87 0.3831397156255818 2307 88-89 0.29088940405312513 2307 90-91 0.11658225311320791 2307 92-93 0.41708436364008605 2307 94-95 0.18359829262939797 2307 96-97 0.31344837336489206 2307 98-99 0.2857640553242575 2307 100-101 0.1569053960010649 2201 1 0.36634804959902567 2201 2 0.2929242608241722 2201 3 0.2931564236624986 2201 4 0.33557737092283446 2201 5 0.3649520507578643 2201 6 0.3896499115957326 2201 7 0.4213310902778318 2201 8 0.3299928225534785 2201 9 0.5081844079700986 2201 10-11 0.46861923606199696 2201 12-13 0.45328970504652943 2201 14-15 0.6455376448068009 2201 16-17 0.6326994699267985 2201 18-19 0.5744889168698251 2201 20-21 0.5121945467251905 2201 22-23 0.4642474088577373 2201 24-25 0.6747608125222229 2201 26-27 0.5143191972505647 2201 28-29 0.5627671621454482 2201 30-31 0.49916238607212193 2201 32-33 0.5286797993826227 2201 34-35 0.6243529913426968 2201 36-37 0.46800682957511697 2201 38-39 0.5618054628678095 2201 40-41 0.3711568417503628 2201 42-43 0.5488479083577786 2201 44-45 0.6584021513349754 2201 46-47 0.5370639270730209 2201 48-49 0.5891274292698085 2201 50-51 0.4402202032701581 2201 52-53 0.42854064524889424 2201 54-55 0.5415130192440856 2201 56-57 0.6938096747664275 2201 58-59 0.5334301203086582 2201 60-61 0.6037364017991464 2201 62-63 0.66839448205792 2201 64-65 0.6144455881477597 2201 66-67 0.5343777561102669 2201 68-69 0.5830619729895972 2201 70-71 0.5527543802905228 2201 72-73 0.49011377544096035 2201 74-75 0.5715302664602717 2201 76-77 0.5920487944929747 2201 78-79 0.44809244449087515 2201 80-81 0.6094337663058695 2201 82-83 0.6768146142603158 2201 84-85 0.6940732937991498 2201 86-87 0.7364753941746756 2201 88-89 0.6570409049912058 2201 90-91 0.6312038916372842 2201 92-93 0.5145554393123817 2201 94-95 0.6567322826231461 2201 96-97 0.6426142582929657 2201 98-99 0.584673529995932 2201 100-101 0.5373885104475953 1101 1 0.34937770577040084 1101 2 0.284607318433892 1101 3 0.3509419945501513 1101 4 0.42577228337741246 1101 5 0.3851721214041035 1101 6 0.4014808688776341 1101 7 0.4515605634805482 1101 8 0.44915879038148887 1101 9 0.506514310640938 1101 10-11 0.45645512333296523 1101 12-13 0.4870812224158456 1101 14-15 0.580502956997087 1101 16-17 0.6833642560821716 1101 18-19 0.620053959689578 1101 20-21 0.6270597343951323 1101 22-23 0.6008642673873794 1101 24-25 0.4983952817860313 1101 26-27 0.5580802198421182 1101 28-29 0.429398251571385 1101 30-31 0.4314550851140737 1101 32-33 0.41328730618042187 1101 34-35 0.38721645097523805 1101 36-37 0.39430628829281034 1101 38-39 0.5083765792307986 1101 40-41 0.47188719167793636 1101 42-43 0.48248949306973543 1101 44-45 0.4994356701782081 1101 46-47 0.34353720602400273 1101 48-49 0.33011673068527614 1101 50-51 0.3904740722950848 1101 52-53 0.4372901052372029 1101 54-55 0.41011000534355446 1101 56-57 0.5240076366987765 1101 58-59 0.48300039806525774 1101 60-61 0.6469848580010193 1101 62-63 0.605973450826184 1101 64-65 0.5992993402760476 1101 66-67 0.5398012523365381 1101 68-69 0.4886092271889808 1101 70-71 0.39251220093040473 1101 72-73 0.4991730115467803 1101 74-75 0.42417568843175957 1101 76-77 0.46532291713280216 1101 78-79 0.3725667907590804 1101 80-81 0.4241884104063942 1101 82-83 0.567753853415617 1101 84-85 0.6432857661632525 1101 86-87 0.6452741540537161 1101 88-89 0.6856760095628829 1101 90-91 0.4809251185438441 1101 92-93 0.563587230118209 1101 94-95 0.5912195891718994 1101 96-97 0.4116844216941544 1101 98-99 0.4099333709148354 1101 100-101 0.48398656386427774 2304 1 0.16353693036999317 2304 2 0.17045826910445072 2304 3 0.08717792454967466 2304 4 0.17043037861166255 2304 5 0.18180276223544212 2304 6 0.16297141596201214 2304 7 0.09182755898325468 2304 8 0.1332457884930065 2304 9 0.18271525026213453 2304 10-11 0.24745025578854296 2304 12-13 0.29679560213451595 2304 14-15 0.2772744154987947 2304 16-17 0.2639585590963378 2304 18-19 0.3025632306157817 2304 20-21 0.2714082683269723 2304 22-23 0.31204269814556795 2304 24-25 0.30151758808497675 2304 26-27 0.4349423054856132 2304 28-29 0.39110449645293244 2304 30-31 0.27489734762797724 2304 32-33 0.333881761599514 2304 34-35 0.3907194109927019 2304 36-37 0.37614376726429555 2304 38-39 0.24593238072607448 2304 40-41 0.26345653210974973 2304 42-43 0.09772554813094558 2304 44-45 0.19700041873921492 2304 46-47 0.05985347583272471 2304 48-49 0.12629508748224794 2304 50-51 0.1624367429493887 2304 52-53 0.3149529891239311 2304 54-55 0.2755919254125416 2304 56-57 0.31996769584981877 2304 58-59 0.29498841276247134 2304 60-61 0.3477926937658893 2304 62-63 0.27666640913582086 2304 64-65 0.2966861336718907 2304 66-67 0.3158483750435721 2304 68-69 0.27318374176807936 2304 70-71 0.32519740989773993 2304 72-73 0.25098842318128334 2304 74-75 0.23505181953406407 2304 76-77 0.3264199457298034 2304 78-79 0.2746700459938509 2304 80-81 0.15292713860520735 2304 82-83 0.2244801328213626 2304 84-85 0.3775363978592665 2304 86-87 0.2908458495899069 2304 88-89 0.31579294896713606 2304 90-91 0.4407954443631681 2304 92-93 0.22659315284263215 2304 94-95 0.37536568901774814 2304 96-97 0.3587708877331792 2304 98-99 0.2772647696020911 2304 100-101 0.28208948111871734 2202 1 0.24727638385469675 2202 2 0.1806285589241554 2202 3 0.14471886601630857 2202 4 0.22312533255352918 2202 5 0.22199503175767887 2202 6 0.3371591349862513 2202 7 0.282278793653596 2202 8 0.217084687523041 2202 9 0.21023522885185741 2202 10-11 0.2558803502475726 2202 12-13 0.27784675171688633 2202 14-15 0.3430106663895316 2202 16-17 0.2782452640607218 2202 18-19 0.27349025426145346 2202 20-21 0.31280974687276597 2202 22-23 0.2651416165684921 2202 24-25 0.2884130445827324 2202 26-27 0.2780786512258473 2202 28-29 0.39655935915707374 2202 30-31 0.28802629494502696 2202 32-33 0.40691190600502125 2202 34-35 0.37048885188503533 2202 36-37 0.4282904488335504 2202 38-39 0.24467324556473358 2202 40-41 0.32756179471106606 2202 42-43 0.26117935089605737 2202 44-45 0.41835603438239133 2202 46-47 0.39274184627612385 2202 48-49 0.40839647557123726 2202 50-51 0.43540490166528656 2202 52-53 0.368142425363267 2202 54-55 0.42417719559531974 2202 56-57 0.3748627553788637 2202 58-59 0.3481372776596956 2202 60-61 0.28153247263478676 2202 62-63 0.4079100234709472 2202 64-65 0.3967479770059015 2202 66-67 0.502247152900523 2202 68-69 0.5442411834673635 2202 70-71 0.40492833343570567 2202 72-73 0.4487081307259615 2202 74-75 0.43513015577958214 2202 76-77 0.3928991910987705 2202 78-79 0.46750929562535504 2202 80-81 0.6016372343006964 2202 82-83 0.5466160807794083 2202 84-85 0.4852679534560309 2202 86-87 0.42919860022522016 2202 88-89 0.6910235650170264 2202 90-91 0.42415814362031057 2202 92-93 0.4673051626106641 2202 94-95 0.4430535333149024 2202 96-97 0.40693334148794946 2202 98-99 0.5279300786031982 2202 100-101 0.7596189568596969 1102 1 0.21510597318990676 1102 2 0.17971085210965043 1102 3 0.33987677087282364 1102 4 0.3268320108634484 1102 5 0.3825284313186543 1102 6 0.35924959179674687 1102 7 0.3625200572129046 1102 8 0.2550399357965816 1102 9 0.20159312249240458 1102 10-11 0.2369808373411857 1102 12-13 0.24564479613213308 1102 14-15 0.3429318541609234 1102 16-17 0.2501090981604861 1102 18-19 0.2847327635947394 1102 20-21 0.302181412124078 1102 22-23 0.3428985424309161 1102 24-25 0.4305765854649479 1102 26-27 0.40547846856573955 1102 28-29 0.22730373915418767 1102 30-31 0.2773732249733811 1102 32-33 0.37572679841180445 1102 34-35 0.3344489310377483 1102 36-37 0.39841971290559286 1102 38-39 0.26189226544904187 1102 40-41 0.07338915286909042 1102 42-43 0.14544604667084116 1102 44-45 0.2255262447619728 1102 46-47 0.1995891969034176 1102 48-49 0.22814119204750227 1102 50-51 0.301746672346745 1102 52-53 0.27338959733615553 1102 54-55 0.40407997715625754 1102 56-57 0.3386226494680429 1102 58-59 0.25410797993974654 1102 60-61 0.2856945995034508 1102 62-63 0.3637870924171267 1102 64-65 0.5334439320458841 1102 66-67 0.5485442800095299 1102 68-69 0.5638220666450096 1102 70-71 0.636913480284818 1102 72-73 0.5106859871935754 1102 74-75 0.49273979190142114 1102 76-77 0.45511258296878765 1102 78-79 0.5187417506413041 1102 80-81 0.4688998162173448 1102 82-83 0.45436301241004884 1102 84-85 0.4655175288514002 1102 86-87 0.41607050581878724 1102 88-89 0.6059912790418096 1102 90-91 0.5692331304014928 1102 92-93 0.6125681170577977 1102 94-95 0.4992918166303397 1102 96-97 0.56083880286495 1102 98-99 0.6552777216468897 1102 100-101 0.6819993265060731 2305 1 0.2124041121805078 2305 2 0.2334696758611159 2305 3 0.20802821853429876 2305 4 0.2457186138606886 2305 5 0.3067621333306576 2305 6 0.2345695117695712 2305 7 0.31020983864810603 2305 8 0.24518187079337395 2305 9 0.19785411679234954 2305 10-11 0.2697948118985849 2305 12-13 0.24955980500306651 2305 14-15 0.19138510243391949 2305 16-17 0.165401555976338 2305 18-19 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-1.1312176352620256 1213 52-53 -1.1212723815054062 1213 54-55 -1.1763674035152718 1213 56-57 -1.1660371784453005 1213 58-59 -1.1659259638150345 1213 60-61 -1.1354502645820688 1213 62-63 -1.2014711398422122 1213 64-65 -1.0847632723858993 1213 66-67 -1.2601901001915152 1213 68-69 -1.116024355840679 1213 70-71 -1.1499162684780941 1213 72-73 -1.0332401820404655 1213 74-75 -1.161191478813663 1213 76-77 -1.2005238717312423 1213 78-79 -1.0753043312910862 1213 80-81 -1.1624191242862452 1213 82-83 -1.175469742073581 1213 84-85 -1.1582326857765253 1213 86-87 -1.0718875194679427 1213 88-89 -0.9878248953185107 1213 90-91 -1.201904574254371 1213 92-93 -1.3260577187513718 1213 94-95 -1.1557162818933264 1213 96-97 -1.2176367283120406 1213 98-99 -1.2698595007547482 1213 100-101 -1.2473199326076347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1876.0 3 247.0 4 181.0 5 172.0 6 284.0 7 1601.0 8 6170.0 9 9800.0 10 10690.0 11 9428.0 12 7309.0 13 5775.0 14 4836.0 15 4479.0 16 4367.0 17 4830.0 18 5409.0 19 5981.0 20 6675.0 21 8012.0 22 9156.0 23 9973.0 24 11194.0 25 13029.0 26 15385.0 27 18079.0 28 22072.0 29 27154.0 30 34677.0 31 45211.0 32 61911.0 33 88914.0 34 136352.0 35 232959.0 36 470579.0 37 888101.0 38 338789.0 39 716.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.84781232099606 24.830769854554894 26.229012937773312 13.092404886675727 2 31.595255763263523 22.216481139151004 35.15772587927006 11.030537218315416 3 39.908815676551924 18.454117796637107 26.80187374845046 14.83519277836051 4 30.659310750378953 17.870704606453845 36.93971896797316 14.530265675194046 5 30.496581289644205 16.72513536948538 38.158180227193434 14.620103113676988 6 23.446806024765266 17.44417602182847 44.57282719752085 14.536190755885418 7 33.9547558629096 17.16939475169917 32.65891050617668 16.216938879214545 8 30.038509228428893 28.096282208720574 26.577009120564917 15.288199442285618 9 30.75290215588723 27.706775503718927 24.858779952532746 16.681542387861093 10-11 24.71808367194801 28.810690996351262 30.59309960811913 15.8781257235816 12-13 25.838000506527752 26.34575981645818 29.92466641174747 17.891573265266597 14-15 23.79009191106365 27.25584534660514 31.198554275834887 17.75550846649632 16-17 21.98407748322534 28.842439386776526 32.51097453906936 16.662508590928773 18-19 21.077234113168334 29.385139339669653 32.322247056129335 17.21537949103268 20-21 21.081887374074427 29.522416340928594 31.816744526011377 17.578951758985603 22-23 21.220488575056926 29.03764580492419 32.01366656845078 17.728199051568104 24-25 20.809321319043608 29.59779324169314 31.583959373593913 18.008926065669346 26-27 20.479716722861873 29.9723409405194 31.170111270683883 18.377831065934846 28-29 20.244990254217242 29.808976297254524 30.911523876273282 19.03450957225495 30-31 20.119212417237893 30.081893436359746 30.65730226744514 19.141591878957215 32-33 19.563808906380196 30.165058178036126 31.90178839541576 18.36934452016792 34-35 19.891788979760744 30.144870645314796 31.67244450993529 18.290895864989174 36-37 20.045192922956883 30.627352058198813 31.550021017652043 17.777434001192262 38-39 20.749247727070667 30.09425861958887 30.773089344107905 18.383404309232557 40-41 20.223808382471262 29.69616202040173 30.639789239813307 19.440240357313705 42-43 20.32335159909846 30.200594434947536 31.20962557204464 18.266428393909358 44-45 19.906296259607974 30.450513134794704 31.435647601691073 18.207543003906245 46-47 20.27844481093962 30.071406869934204 31.180582042890283 18.46956627623589 48-49 20.105171258361214 30.470583297478594 30.557569587540172 18.86667585662002 50-51 19.938051100679296 30.12585558759733 30.401597428452163 19.534495883271212 52-53 20.383844038516898 29.941728899628924 30.292696347905384 19.381730713948798 54-55 20.837847325465876 29.96003289653602 30.0656836821049 19.136436095893202 56-57 20.921972269045334 30.27424548468685 29.850370313775866 18.95341193249195 58-59 20.708741587893726 30.187674419159826 30.450889691892307 18.652694301054133 60-61 20.16420182298865 30.66856042602426 30.033135485595352 19.134102265391732 62-63 20.43906089576121 30.553454677905634 29.96279657168579 19.044687854647364 64-65 20.138853678715805 30.367645903832386 30.259321184978578 19.234179232473238 66-67 20.3911406050432 29.564206870344222 30.274252604631176 19.770399919981404 68-69 20.339359213535232 29.717327930504606 30.891182359986928 19.052130495973234 70-71 19.680596514618713 30.774068139754828 30.561353832426295 18.983981513200156 72-73 19.498780763259695 31.315733800773693 30.081584673724933 19.103900762241675 74-75 20.052196261282727 30.62207835046135 29.90357450543088 19.42215088282504 76-77 20.084397481397502 30.313481188325124 30.1694451530701 19.432676177207274 78-79 19.90201195621159 30.502218550321768 30.449605651810387 19.146163841656254 80-81 19.20509281540732 30.828576883454712 30.312036370434303 19.654293930703663 82-83 18.668239640165403 30.674634171277877 30.310869131508976 20.34625705704774 84-85 18.716900671682907 30.377689864291014 30.777636559280943 20.12777290474514 86-87 18.84918373537412 30.260737248817748 31.472717417789248 19.417361598018886 88-89 19.07406943315707 30.735745788069472 30.846173093209632 19.344011685563817 90-91 19.28910926093507 30.492530330154416 30.75979639181869 19.458564017091824 92-93 18.726976499602877 31.58584190786524 30.553299608442686 19.133881984089193 94-95 18.46308871485178 31.79205841750919 30.572024660345633 19.1728282072934 96-97 19.012358392794624 31.76102773287518 30.340239000895224 18.88637487343497 98-99 19.33319215316656 30.88765538517827 29.970938758628645 19.808213703026524 100-101 19.163666045592468 30.886474690563436 29.63474631691332 20.315112946930775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1355.0 1 880.5 2 324.5 3 259.0 4 294.0 5 322.0 6 405.5 7 525.0 8 652.0 9 837.0 10 1001.0 11 1108.5 12 1116.0 13 1022.0 14 926.5 15 885.5 16 904.5 17 975.0 18 1102.5 19 1262.5 20 1664.0 21 2351.0 22 3246.0 23 4658.5 24 6551.5 25 9161.5 26 13212.5 27 18450.0 28 24159.0 29 30284.5 30 36847.5 31 43698.5 32 52409.0 33 64531.0 34 80556.0 35 99969.0 36 126607.5 37 156144.5 38 181818.5 39 192967.5 40 184878.0 41 165944.0 42 147314.5 43 137815.0 44 123887.5 45 107696.0 46 100993.0 47 90260.0 48 70244.5 49 52589.5 50 40036.5 51 31912.5 52 26545.0 53 20501.5 54 15096.5 55 11147.0 56 8065.5 57 5790.5 58 4206.0 59 3124.0 60 2277.5 61 1675.0 62 1307.0 63 1009.0 64 741.5 65 541.0 66 421.0 67 325.5 68 248.0 69 194.5 70 150.5 71 129.0 72 106.0 73 81.5 74 67.5 75 57.5 76 46.5 77 36.5 78 27.0 79 23.0 80 19.0 81 17.0 82 11.5 83 5.5 84 5.5 85 5.5 86 5.5 87 3.5 88 4.0 89 6.0 90 3.0 91 0.5 92 0.5 93 1.0 94 1.0 95 0.5 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2662572109676087 2 0.25349145427738085 3 0.21777112266901047 4 0.22114096527357374 5 0.2349771425558393 6 0.23890201805997763 7 0.21432198965022223 8 0.2106746305958714 9 0.19219996408144235 10-11 0.17903775532008945 12-13 0.2075624818375395 14-15 0.20445033307920757 16-17 0.20548110846413278 18-19 0.19749259923096227 20-21 0.1987414232550063 22-23 0.16298144643952342 24-25 0.4790925053511118 26-27 0.33177091572102935 28-29 0.2745827044612355 30-31 0.2787454512080489 32-33 0.32144333926822083 34-35 0.31821225488855137 36-37 0.31002551961981833 38-39 0.33664727619586793 40-41 0.34156328187781904 42-43 0.3792658738418148 44-45 0.34515117312150106 46-47 0.3718522201117757 48-49 0.34723254649490776 50-51 0.37004836318815654 52-53 0.35817462365796016 54-55 0.3342289185620049 56-57 0.37143594543709435 58-59 0.35803586543306637 60-61 0.3231084379669462 62-63 0.29936095890655345 64-65 0.3057240146481111 66-67 0.31547673559778827 68-69 0.32249393725670233 70-71 0.29723994032603424 72-73 0.30467341665962966 74-75 0.30263168849333544 76-77 0.30623940234057373 78-79 0.3084595339388742 80-81 0.3267954422284095 82-83 0.34342660661210694 84-85 0.3384907783265996 86-87 0.3423561860200692 88-89 0.3370635508705493 90-91 0.3107193107442872 92-93 0.2939890333428085 94-95 0.30318672139291053 96-97 0.2918481921587331 98-99 0.3114329244723124 100-101 0.33496235489358633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2522373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.44468026250581 #Duplication Level Percentage of deduplicated Percentage of total 1 92.46689438743634 56.815987605029186 2 3.5386942937084602 4.348678788473403 3 1.1753234555497882 2.1665212179384064 4 0.6280296116226184 1.5435631472614997 5 0.39355260526041025 1.2090856998351034 6 0.28746180545687033 1.0597799234388026 7 0.19257523255778608 0.8282906513693606 8 0.13788842444771823 0.6778008121672588 9 0.12066224305592951 0.6672647649895515 >10 0.8540630502455624 10.266727876629778 >50 0.09795744893576679 4.213071789795479 >100 0.10021471821515608 12.196092136999305 >500 0.00536895813653123 2.2484725759398505 >1k 0.0011836683596480766 1.136467978793632 >5k 1.3009701146683613E-4 0.6221950313396152 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7822 0.31010481003404333 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7745 0.30705212908638013 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 4114 0.16310038206086094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.5680686401257864E-4 0.0 0.0 2 3.5680686401257864E-4 0.0 0.0 3 3.5680686401257864E-4 0.0 0.0 4 4.360972782375961E-4 0.0 0.0 5 5.94678106687631E-4 0.0 0.0 6 6.343233138001397E-4 0.0 0.0 7 6.343233138001397E-4 0.0 3.9645207112508734E-5 8 8.721945564751922E-4 0.0 3.9645207112508734E-5 9 0.0011100657991502446 0.0 7.929041422501747E-5 10-11 0.0016849213022816214 0.0 7.929041422501747E-5 12-13 0.0022003089947442347 0.0 7.929041422501747E-5 14-15 0.0027751644978756115 0.0 7.929041422501747E-5 16-17 0.003667181657907058 0.0 7.929041422501747E-5 18-19 0.004539376214382251 0.0 9.911301778127184E-5 20-21 0.005689087220645004 0.0 1.1893562133752621E-4 22-23 0.007116314676695318 0.0 1.1893562133752621E-4 24-25 0.009217510653658282 0.0 1.9822603556254368E-4 26-27 0.011596223080408806 0.0 1.9822603556254368E-4 28-29 0.013538838228921733 0.0 1.9822603556254368E-4 30-31 0.021685928290542278 0.0 2.1804863911879807E-4 32-33 0.02412410852796157 0.0 2.3787124267505243E-4 34-35 0.027018208647174705 0.0 2.3787124267505243E-4 36-37 0.0301898252161754 0.0 2.7751644978756115E-4 38-39 0.03395611989186373 0.0 3.1716165690006987E-4 40-41 0.03851531870980224 0.0 3.369842604563243E-4 42-43 0.04315380794196576 0.0 3.5680686401257864E-4 44-45 0.04828786226303564 0.0 3.5680686401257864E-4 46-47 0.05385801386234312 0.0 3.5680686401257864E-4 48-49 0.059368697650981836 0.0 3.5680686401257864E-4 50-51 0.06577139859965199 0.0 3.5680686401257864E-4 52-53 0.07266966463722851 0.0 3.9645207112508737E-4 54-55 0.07958775327836129 0.0 3.9645207112508737E-4 56-57 0.08694193919773166 0.0 3.9645207112508737E-4 58-59 0.09538636831269602 0.0 3.9645207112508737E-4 60-61 0.10452458855212929 0.0 3.9645207112508737E-4 62-63 0.11419801908758141 0.0 3.9645207112508737E-4 64-65 0.12428772429771488 0.0 3.9645207112508737E-4 66-67 0.13526944666787982 0.0 4.162746746813417E-4 68-69 0.14720265400874494 0.0 4.360972782375961E-4 70-71 0.1614749285692481 0.0 4.360972782375961E-4 72-73 0.17774928608893292 0.0 4.360972782375961E-4 74-75 0.19493548337220545 0.0 4.5591988179385045E-4 76-77 0.2160663787631726 0.0 4.7574248535010486E-4 78-79 0.23981385782356535 0.0 4.7574248535010486E-4 80-81 0.26508767735778965 0.0 4.7574248535010486E-4 82-83 0.2928789675436583 0.0 5.153876924626136E-4 84-85 0.32917415465516003 0.0 5.153876924626136E-4 86-87 0.3701474762059378 0.0 5.35210296018868E-4 88-89 0.41679006237380434 0.0 6.343233138001397E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 405 0.0 58.562428 9 CGGGAGA 555 0.0 53.862904 4 ATCCGGA 160 0.0 50.424335 2 TTGTGTT 34020 0.0 46.76255 1 AGAGCGG 535 0.0 43.447388 8 TCGGGAG 720 0.0 42.8391 3 ATTCGGA 135 0.0 42.185062 2 GATCCGG 230 0.0 41.26964 1 CGACGGG 70 4.3372274E-6 40.680073 1 GGCGCCG 280 0.0 39.867043 56-57 GCACGCC 60 8.334746E-5 39.55007 1 CGGAAAG 270 0.0 38.663494 5 GTAGCGC 50 0.0016228694 37.968067 1 CGGCGTG 180 0.0 36.899464 12-13 GTTGCGC 65 1.3352213E-4 36.507755 1 TGTGTTT 56825 0.0 35.579403 1 CGTCGGC 55 0.0025928854 34.51094 3 CGGTGCG 70 2.0641921E-4 33.90006 1 ACGACGC 70 4.620233E-10 33.888947 16-17 AGCGGCG 400 0.0 33.800865 10-11 >>END_MODULE