FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l04n02_50330665-2.34200000035755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l04n02_50330665-2.34200000035755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522373
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG78220.31010481003404333No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC77450.30705212908638013No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA41140.16310038206086094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC4050.058.5624289
CGGGAGA5550.053.8629044
ATCCGGA1600.050.4243352
TTGTGTT340200.046.762551
AGAGCGG5350.043.4473888
TCGGGAG7200.042.83913
ATTCGGA1350.042.1850622
GATCCGG2300.041.269641
CGACGGG704.3372274E-640.6800731
GGCGCCG2800.039.86704356-57
GCACGCC608.334746E-539.550071
CGGAAAG2700.038.6634945
GTAGCGC500.001622869437.9680671
CGGCGTG1800.036.89946412-13
GTTGCGC651.3352213E-436.5077551
TGTGTTT568250.035.5794031
CGTCGGC550.002592885434.510943
CGGTGCG702.0641921E-433.900061
ACGACGC704.620233E-1033.88894716-17
AGCGGCG4000.033.80086510-11
Produced by FastQC (version 0.11.2)